(Circular) (Six-base) MAPSORT of: pFN21AE3526.seq Check: 8639 from: 1 to: 7813 pFN21AE3526 7813 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3808 3808 Size: 7813 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2159 3579 3939 5623 Size: 53 83 186 936 623 1420 360 1684 Cuts at: 5623 278 Size: 2468 Fragments arranged by size: 2468 1684 1420 936 623 360 186 83 53 AccI GT'mk_AC Cuts at: 1937 5156 1937 Size: 3219 4594 Acc65I G'GTAC_C Cuts at: 2562 5134 2562 Size: 2572 5241 AclI AA'CG_TT Cuts at: 1563 5940 6313 1563 Size: 4377 373 3063 Fragments arranged by size: 4377 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2297 3827 4595 4991 5876 7260 7528 Size: 404 261 1530 768 396 885 1384 268 Cuts at: 7528 1632 Size: 1917 Fragments arranged by size: 1917 1530 1384 885 768 404 396 268 261 AfeI AGC'GCT Cuts at: 2964 3401 2964 Size: 437 7376 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7616 AflIII A'CryG_T Cuts at: 1203 3351 6712 7689 1203 Size: 2148 3361 977 1327 Fragments arranged by size: 3361 2148 1327 977 AgeI A'CCGG_T Cuts at: 6644 6644 Size: 7813 AhdI GACnn_n'nnGTC Cuts at: 6542 6542 Size: 7813 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3978 4377 1184 Size: 2794 399 4620 Fragments arranged by size: 4620 2794 399 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3689 3721 3689 Size: 32 7781 AlwNI CAG_nnn'CTG Cuts at: 3313 4184 4849 5556 7128 3313 Size: 871 665 707 1572 3998 Fragments arranged by size: 3998 1572 871 707 665 ApaI G_GGCC'C Cuts at: 2603 3932 4520 4604 4694 2603 Size: 1329 588 84 90 5722 Fragments arranged by size: 5722 1329 588 90 84 ApaLI G'TGCA_C Cuts at: 2111 2771 4589 5870 7026 2111 Size: 660 1818 1281 1156 2898 Fragments arranged by size: 2898 1818 1281 1156 660 ApoI r'AATT_y Cuts at: 1493 3329 5122 5348 5500 7609 1493 Size: 1836 1793 226 152 2109 1697 Fragments arranged by size: 2109 1836 1793 1697 226 152 AscI GG'CGCG_CC Cuts at: 4077 4077 Size: 7813 AseI AT'TA_AT Cuts at: 160 1032 6367 160 Size: 872 5335 1606 Fragments arranged by size: 5335 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 AvaI C'yCGr_G Cuts at: 1870 1943 2720 3425 3811 3993 5138 1870 Size: 73 777 705 386 182 1145 4545 Fragments arranged by size: 4545 1145 777 705 386 182 73 AvrII C'CTAG_G Cuts at: 2944 2944 Size: 7813 BamHI G'GATC_C Cuts at: 2995 3564 5143 5510 2995 Size: 569 1579 367 5298 Fragments arranged by size: 5298 1579 569 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2562 4064 4102 4345 5134 Size: 325 217 627 775 1502 38 243 789 Cuts at: 5134 6589 618 Size: 1455 1842 Fragments arranged by size: 1842 1502 1455 789 775 627 325 243 217 38 BanII G_rGCy'C Cuts at: 1391 2603 2727 3483 3650 3932 4208 4520 4604 Size: 1212 124 756 167 282 276 312 84 Cuts at: 4604 4619 4694 4737 5132 1391 Size: 15 75 43 395 4072 Fragments arranged by size: 4072 1212 756 395 312 282 276 167 124 84 75 43 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3088 4683 928 Size: 971 1189 1595 4058 Fragments arranged by size: 4058 1595 1189 971 BbvCI CC'TCA_GC Cuts at: 2078 2078 Size: 7813 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6004 6038 1903 Size: 34 50 34 3983 34 3678 Fragments arranged by size: 3983 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3550 5705 6915 3550 Size: 2155 1210 4448 Fragments arranged by size: 4448 2155 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7813 BfrBI ATG'CAT Cuts at: 5110 5110 Size: 7813 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2534 2891 4087 Size: 107 122 71 631 605 862 357 1196 Cuts at: 4087 6424 136 Size: 2337 1525 Fragments arranged by size: 2337 1525 1196 862 631 605 357 122 107 71 BglII A'GATC_T Cuts at: 3414 7808 3414 Size: 4394 3419 BlpI GC'TnA_GC Cuts at: 2045 2196 2679 3969 4451 4736 5230 2045 Size: 151 483 1290 482 285 494 4628 Fragments arranged by size: 4628 1290 494 483 482 285 151 Bme1580I G_kGCm'C Cuts at: 1790 2115 2603 2775 3932 4520 4593 4604 4694 Size: 325 488 172 1157 588 73 11 90 Cuts at: 4694 5086 5874 7030 1790 Size: 392 788 1156 2573 Fragments arranged by size: 2573 1157 1156 788 588 488 392 325 172 90 73 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3557 3998 4515 Size: 416 53 83 399 24 2140 441 517 Cuts at: 4515 5544 6502 442 Size: 1029 958 1753 Fragments arranged by size: 2140 1753 1029 958 517 441 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7813 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2044 3451 3934 2044 Size: 1407 483 5923 Fragments arranged by size: 5923 1407 483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3431 3469 3545 3869 4820 4877 4895 6473 Size: 2192 38 76 324 951 57 18 1578 Cuts at: 6473 1239 Size: 2579 Fragments arranged by size: 2579 2192 1578 951 324 76 57 38 18 Bpu10I CC'TnA_GC Cuts at: 2078 4202 4718 2078 Size: 2124 516 5173 Fragments arranged by size: 5173 2124 516 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 2894 3029 4462 5938 6803 7101 2363 Size: 531 135 1433 1476 865 298 3075 Fragments arranged by size: 3075 1476 1433 865 531 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2459 3422 4805 6476 882 Size: 617 960 963 1383 1671 2219 Fragments arranged by size: 2219 1671 1383 963 960 617 BsaAI yAC'GTr Cuts at: 493 1204 5604 493 Size: 711 4400 2702 Fragments arranged by size: 4400 2702 711 BsaBI GATnn'nnATC Cuts at: 2618 7807 2618 Size: 5189 2624 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2156 2576 2940 3576 Size: 53 83 186 936 623 420 364 636 Cuts at: 3576 3891 3936 4436 4493 5620 6002 6637 275 Size: 315 45 500 57 1127 382 635 1451 Fragments arranged by size: 1451 1127 936 636 635 623 500 420 382 364 315 186 83 57 53 45 BsaWI w'CCGG_w Cuts at: 3707 6246 6644 6918 7065 7476 3707 Size: 2539 398 274 147 411 4044 Fragments arranged by size: 4044 2539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 3620 3650 1178 Size: 30 165 30 1143 30 1044 30 5341 Fragments arranged by size: 5341 1143 1044 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2765 2915 4238 4286 4390 4817 4847 4892 Size: 1579 150 1323 48 104 427 30 45 Cuts at: 4892 4901 1186 Size: 9 4098 Fragments arranged by size: 4098 1579 1323 427 150 104 48 45 30 9 BseYI C'CCAG_C Cuts at: 2051 2999 3214 3671 4092 4246 5091 7016 7512 Size: 948 215 457 421 154 845 1925 496 Cuts at: 7512 2051 Size: 2352 Fragments arranged by size: 2352 1925 948 845 496 457 421 215 154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2733 3163 3476 1163 Size: 573 997 430 313 5500 Fragments arranged by size: 5500 997 573 430 313 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3015 3157 4432 5263 6024 7052 Size: 275 186 1014 142 1275 831 761 1028 Cuts at: 7052 1540 Size: 2301 Fragments arranged by size: 2301 1275 1028 1014 831 761 275 186 142 BsiHKAI G_wGCw'C Cuts at: 2115 2271 2355 2409 2685 2775 2789 2859 3011 Size: 156 84 54 276 90 14 70 152 Cuts at: 3011 3033 3483 3596 4208 4457 4593 4737 5874 Size: 22 450 113 612 249 136 144 1137 Cuts at: 5874 5959 7030 2115 Size: 85 1071 2898 Fragments arranged by size: 2898 1137 1071 612 450 276 249 156 152 144 136 113 90 85 84 70 54 22 14 BsmI GAATG_Cn' Cuts at: 5324 5417 5324 Size: 93 7720 BsmBI CGTCTCn'nnnn_ Cuts at: 3741 4829 3741 Size: 1088 6725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2271 2355 2409 2603 2685 2727 Size: 399 325 156 84 54 194 82 42 Cuts at: 2727 2775 2789 2859 3011 3033 3483 3596 3650 Size: 48 14 70 152 22 450 113 54 Cuts at: 3650 3932 4208 4457 4520 4593 4604 4619 4694 Size: 282 276 249 63 73 11 15 75 Cuts at: 4694 4737 5086 5132 5874 5959 7030 1391 Size: 43 349 46 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 349 325 282 276 249 194 156 152 113 85 84 82 75 73 70 63 54 54 48 46 43 42 22 15 14 11 BspEI T'CCGG_A Cuts at: 7476 7476 Size: 7813 BspHI T'CATG_A Cuts at: 3360 5076 5702 7432 3360 Size: 1716 626 1730 3741 Fragments arranged by size: 3741 1730 1716 626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2313 2530 2940 3252 3808 4455 4935 5168 Size: 1469 217 410 312 556 647 480 233 Cuts at: 5168 844 Size: 3489 Fragments arranged by size: 3489 1469 647 556 480 410 312 233 217 BsrBI CCG'CTC Cuts at: 1394 1823 2200 2231 3175 4108 4891 5700 1394 Size: 429 377 31 944 933 783 809 3507 Fragments arranged by size: 3507 944 933 809 783 429 377 31 BsrDI GCAATG_nn' Cuts at: 65 1248 5043 6308 6482 65 Size: 1183 3795 1265 174 1396 Fragments arranged by size: 3795 1396 1265 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2184 2256 3446 3988 4086 6457 6644 Size: 110 374 72 1190 542 98 2371 187 Cuts at: 6644 1700 Size: 2869 Fragments arranged by size: 2869 2371 1190 542 374 187 110 98 72 BsrGI T'GTAC_A Cuts at: 96 2664 96 Size: 2568 5245 BssHII G'CGCG_C Cuts at: 1925 2627 4077 1925 Size: 702 1450 5661 Fragments arranged by size: 5661 1450 702 BssSI C'ACGA_G Cuts at: 2033 3127 5873 6885 2033 Size: 1094 2746 1012 2961 Fragments arranged by size: 2961 2746 1094 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 3499 794 Size: 1985 720 5108 Fragments arranged by size: 5108 1985 720 BstBI TT'CG_AA Cuts at: 6617 6617 Size: 7813 BstEII G'GTnAC_C Cuts at: 1184 3301 3729 1184 Size: 2117 428 5268 Fragments arranged by size: 5268 2117 428 BstXI CCAn_nnnn'nTGG Cuts at: 3631 4627 4798 3631 Size: 996 171 6646 Fragments arranged by size: 6646 996 171 BstYI r'GATC_y Cuts at: 1972 2995 3414 3564 5143 5510 5895 5912 7364 Size: 1023 419 150 1579 367 385 17 1452 Cuts at: 7364 7450 7462 7808 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1579 1452 1023 419 385 367 346 150 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3569 4532 3569 Size: 963 6850 BtgI C'CryG_G Cuts at: 513 1065 2367 2655 3101 3873 4026 4162 4266 Size: 552 1302 288 446 772 153 136 104 Cuts at: 4266 4341 4349 4426 4580 4956 513 Size: 75 8 77 154 376 3370 Fragments arranged by size: 3370 1302 772 552 446 376 288 154 153 136 104 77 75 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3277 487 Size: 683 56 2051 5023 Fragments arranged by size: 5023 2051 683 56 BtsI GCAGTG_nn' Cuts at: 2062 3270 3497 4260 4593 5329 6123 6143 2062 Size: 1208 227 763 333 736 794 20 3732 Fragments arranged by size: 3732 1208 794 763 736 333 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7813 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3090 3125 529 Size: 35 2526 35 5217 Fragments arranged by size: 5217 2526 35 35 DraI TTT'AAA Cuts at: 5117 5117 Size: 7813 DraIII CAC_nnn'GTG Cuts at: 4163 4163 Size: 7813 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6820 809 Size: 507 5504 1802 Fragments arranged by size: 5504 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 2535 2883 Size: 54 1425 50 275 18 298 407 348 Cuts at: 2883 3012 3154 3315 3849 3978 3986 4023 4429 Size: 129 142 161 534 129 8 37 406 Cuts at: 4429 5260 6149 7778 8 Size: 831 889 1629 43 Fragments arranged by size: 1629 1425 889 831 534 407 406 348 298 275 161 142 129 129 54 50 43 37 18 8 EagI C'GGCC_G Cuts at: 1537 1812 3012 3154 4429 5260 1537 Size: 275 1200 142 1275 831 4090 Fragments arranged by size: 4090 1275 1200 831 275 142 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 3200 3242 4552 5743 1353 Size: 732 1115 42 1310 1191 3423 Fragments arranged by size: 3423 1310 1191 1115 732 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4383 6400 6774 6920 387 Size: 1064 138 2794 2017 374 146 1280 Fragments arranged by size: 2794 2017 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3481 4206 4735 3481 Size: 725 529 6559 Fragments arranged by size: 6559 725 529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2297 3431 3469 3545 3827 3869 Size: 393 404 261 1134 38 76 282 42 Cuts at: 3869 4595 4820 4877 4895 4991 5876 6473 7260 Size: 726 225 57 18 96 885 597 787 Cuts at: 7260 7528 1239 Size: 268 1524 Fragments arranged by size: 1524 1134 885 787 726 597 404 393 282 268 261 225 96 76 57 42 38 18 EcoNI CCTnn'n_nnAGG Cuts at: 2129 3130 3248 2129 Size: 1001 118 6694 Fragments arranged by size: 6694 1001 118 EcoO109I rG'GnC_Cy Cuts at: 2302 2599 2600 3551 3777 3929 4517 4600 4601 Size: 297 1 951 226 152 588 83 1 Cuts at: 4601 4684 4753 2302 Size: 83 69 5362 Fragments arranged by size: 5362 951 588 297 226 152 83 83 69 1 1 EcoRI G'AATT_C Cuts at: 5122 5500 5122 Size: 378 7435 FseI GG_CCGG'CC Cuts at: 3992 4090 3992 Size: 98 7715 FspI TGC'GCA Cuts at: 2438 6319 2438 Size: 3881 3932 HaeII r_GCGC'y Cuts at: 1678 2072 2966 3189 3403 6960 1678 Size: 394 894 223 214 3557 2531 Fragments arranged by size: 3557 2531 894 394 223 214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2598 2630 2744 2776 3620 Size: 32 135 32 1026 32 114 32 844 Cuts at: 3620 3652 4166 4198 6454 6486 6528 6560 1373 Size: 32 514 32 2256 32 42 32 2626 Fragments arranged by size: 2626 2256 1026 844 514 135 114 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5157 5403 669 Size: 988 281 3219 246 3079 Fragments arranged by size: 3219 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7813 HpaI GTT'AAC Cuts at: 5403 5403 Size: 7813 KpnI G_GTAC'C Cuts at: 2566 5138 2566 Size: 2572 5241 MfeI C'AATT_G Cuts at: 5412 5412 Size: 7813 MluI A'CGCG_T Cuts at: 7689 7689 Size: 7813 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3444 3764 5034 6927 7111 1305 Size: 2139 320 1270 1893 184 2007 Fragments arranged by size: 2139 2007 1893 1270 320 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2130 2885 3317 3851 4025 Size: 54 1425 343 298 755 432 534 174 Cuts at: 4025 10 Size: 3798 Fragments arranged by size: 3798 1425 755 534 432 343 298 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 4925 5771 6130 6289 518 Size: 648 1494 2265 846 359 159 2042 Fragments arranged by size: 2265 2042 1494 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2195 2841 3017 3523 3671 3962 3973 4096 Size: 589 646 176 506 148 291 11 123 Cuts at: 4096 4153 4268 4428 5095 5229 5906 7054 7299 Size: 57 115 160 667 134 677 1148 245 Cuts at: 7299 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 667 646 589 506 291 245 176 160 148 134 123 115 57 11 NaeI GCC'GGC Cuts at: 2258 3448 3990 4088 2258 Size: 1190 542 98 5983 Fragments arranged by size: 5983 1190 542 98 NcoI C'CATG_G Cuts at: 513 1065 2655 3873 513 Size: 552 1590 1218 4453 Fragments arranged by size: 4453 1590 1218 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7813 NgoMIV G'CCGG_C Cuts at: 2256 3446 3988 4086 2256 Size: 1190 542 98 5983 Fragments arranged by size: 5983 1190 542 98 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7813 NotI GC'GGCC_GC Cuts at: 3154 5260 3154 Size: 2106 5707 NsiI A_TGCA'T Cuts at: 5112 5112 Size: 7813 NspI r_CATG'y Cuts at: 1132 1229 3355 3397 5114 5171 6716 1132 Size: 97 2126 42 1717 57 1545 2229 Fragments arranged by size: 2229 2126 1717 1545 97 57 42 PasI CC'CwG_GG Cuts at: 1348 3617 1348 Size: 2269 5544 PciI A'CATG_T Cuts at: 3351 6712 3351 Size: 3361 4452 PflMI CCAn_nnn'nTGG Cuts at: 1108 2701 3313 4099 1108 Size: 1593 612 786 4822 Fragments arranged by size: 4822 1593 786 612 PfoI T'CCnGG_A Cuts at: 2868 3885 2868 Size: 1017 6796 PmeI GTTT'AAAC Cuts at: 5117 5117 Size: 7813 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4802 4834 5882 5914 7395 7427 4802 Size: 32 1048 32 1481 32 5188 Fragments arranged by size: 5188 1481 1048 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2302 3551 3777 4753 2302 Size: 1249 226 976 5362 Fragments arranged by size: 5362 1249 976 226 PshAI GACnn'nnGTC Cuts at: 1613 4312 1613 Size: 2699 5114 PsiI TTA'TAA Cuts at: 5383 5383 Size: 7813 PspOMI G'GGCC_C Cuts at: 2599 3928 4516 4600 4690 2599 Size: 1329 588 84 90 5722 Fragments arranged by size: 5722 1329 588 90 84 PspXI vC'TCGA_Gb Cuts at: 2720 3811 2720 Size: 1091 6722 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2657 2689 4112 4144 2657 Size: 32 1423 32 6326 Fragments arranged by size: 6326 1423 32 32 PstI C_TGCA'G Cuts at: 830 2194 2784 2937 3252 3681 3967 4464 4770 Size: 1364 590 153 315 429 286 497 306 Cuts at: 4770 5165 830 Size: 395 3478 Fragments arranged by size: 3478 1364 590 497 429 395 315 306 286 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7813 PvuII CAG'CTG Cuts at: 2195 3523 3671 3973 4096 5095 2195 Size: 1328 148 302 123 999 4913 Fragments arranged by size: 4913 1328 999 302 148 123 SacI G_AGCT'C Cuts at: 3483 4208 4737 3483 Size: 725 529 6559 Fragments arranged by size: 6559 725 529 SacII CC_GC'GG Cuts at: 4269 4429 4269 Size: 160 7653 SalI G'TCGA_C Cuts at: 1936 5155 1936 Size: 3219 4594 SanDI GG'GwC_CC Cuts at: 3551 3551 Size: 7813 SbfI CC_TGCA'GG Cuts at: 2937 5165 2937 Size: 2228 5585 ScaI AGT'ACT Cuts at: 6061 7773 6061 Size: 1712 6101 SfcI C'TryA_G Cuts at: 826 1045 2190 2780 2933 3248 3677 3963 4460 Size: 219 1145 590 153 315 429 286 497 Cuts at: 4460 4766 5161 6296 6977 7168 826 Size: 306 395 1135 681 191 1471 Fragments arranged by size: 1471 1145 1135 681 590 497 429 395 315 306 286 219 191 153 SfiI GGCCn_nnn'nGGCC Cuts at: 2891 2891 Size: 7813 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7813 SmaI CCC'GGG Cuts at: 1872 3995 5140 1872 Size: 2123 1145 4545 Fragments arranged by size: 4545 2123 1145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2378 2720 2909 3044 3811 4477 Size: 197 926 435 342 189 135 767 666 Cuts at: 4477 5917 6818 7080 820 Size: 1440 901 262 1553 Fragments arranged by size: 1553 1440 926 901 767 666 435 342 262 197 189 135 SnaBI TAC'GTA Cuts at: 493 5604 493 Size: 5111 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7813 SphI G_CATG'C Cuts at: 1132 5171 1132 Size: 4039 3774 SrfI GCCC'GGGC Cuts at: 3995 3995 Size: 7813 SspI AAT'ATT Cuts at: 5 52 5737 5 Size: 47 5685 2081 Fragments arranged by size: 5685 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7813 StyI C'CwwG_G Cuts at: 513 1065 2125 2298 2586 2655 2745 2889 2944 Size: 552 1060 173 288 69 90 144 55 Cuts at: 2944 3297 3873 4168 4554 5252 513 Size: 353 576 295 386 698 3074 Fragments arranged by size: 3074 1060 698 576 552 386 353 295 288 173 144 90 69 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 2483 3320 4501 5840 5857 6010 Size: 1255 695 208 837 1181 1339 17 153 Cuts at: 6010 6195 6640 325 Size: 185 445 1498 Fragments arranged by size: 1498 1339 1255 1181 837 695 445 208 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2664 6059 7771 Size: 274 80 33 51 1443 687 3395 1712 Cuts at: 7771 96 Size: 138 Fragments arranged by size: 3395 1712 1443 687 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7773 7805 7773 Size: 32 7781 Tth111I GACn'n_nGTC Cuts at: 1316 2155 1316 Size: 839 6974 XbaI T'CTAG_A Cuts at: 5149 5149 Size: 7813 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2653 2698 2752 4139 4795 2059 Size: 594 45 54 1387 656 5077 Fragments arranged by size: 5077 1387 656 594 54 45 XhoI C'TCGA_G Cuts at: 1943 2720 3811 1943 Size: 777 1091 5945 Fragments arranged by size: 5945 1091 777 XmaI C'CCGG_G Cuts at: 1870 3993 5138 1870 Size: 2123 1145 4545 Fragments arranged by size: 4545 2123 1145 XmnI GAAnn'nnTTC Cuts at: 2804 3368 5942 2804 Size: 564 2574 4675 Fragments arranged by size: 4675 2574 564 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2157 3577 3937 5621 Size: 53 83 186 936 623 1420 360 1684 Cuts at: 5621 276 Size: 2468 Fragments arranged by size: 2468 1684 1420 936 623 360 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: BaeI BbeI BsiWI BstZ17I EcoRV FalI FspAI KasI NarI NruI PacI PmlI RsrII SapI SexAI SfoI SwaI