Restriction map for
pFN21ASDA1737
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbvCI BcgI BcnI
BfrI BglII BlnI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT22I FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 471 GAATTC [GA]^AATT[CT]
AcyI 639 GACGCC G[GA]^CG[CT]C
ApaI 1100 GGGCCC GGGCC^C
ApoI 471 GAATTC [GA]^AATT[CT]
Asp700I 249 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1145 GGTCC G^G[AT]CC
AviII 799 TGCGCA TGC^GCA
BbrPI 748 CACGTG CAC^GTG
BciVI 736 GTATCC GTATCC(6/5)
BclI 1182 TGATCA T^GATCA
BglI 1102 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 189 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 189 GCTGAGC GC^T[ACGT]AGC
BsaAI 748 CACGTG [CT]AC^GT[GA]
BsaHI 639 GACGCC G[GA]^CG[CT]C
BsgI 550 GTGCAG GTGCAG(16/14)
BspHI 281 TCATGA T^CATGA
BspMI 706 GCAGGT ACCTGC(4/8)
BsrFI 732 GCCGGT [GA]^CCGG[CT]
CelII 189 GCTGAGC GC^T[ACGT]AGC
Cfr10I 732 GCCGGT [GA]^CCGG[CT]
EarI 725 CTCTTC CTCTTC(1/4)
EcoRI 471 GAATTC G^AATTC
EcoRV 910 GATATC GAT^ATC
FauI 784 CCCGC CCCGC(4/6)
FbaI 1182 TGATCA T^GATCA
FspI 799 TGCGCA TGC^GCA
HapII 733 CCGG C^CGG
HgaI 639 GACGC GACGC(5/10)
Hin1I 639 GACGCC G[GA]^CG[CT]C
HpaII 733 CCGG C^CGG
Ksp632I 725 CTCTTC CTCTTC(1/4)
MaeII 749 ACGT A^CGT
MseI 1135 TTAA T^TAA
MspI 733 CCGG C^CGG
NspI 185 ACATGC [GA]CATG^[CT]
PmaCI 748 CACGTG CAC^GTG
PmlI 748 CACGTG CAC^GTG
PvuII 1072 CAGCTG CAG^CTG
RcaI 281 TCATGA T^CATGA
TaqI 637 TCGA T^CGA
Tru9I 1135 TTAA T^TAA
TthHB8I 637 TCGA T^CGA
XcmI 740 CCAGCAGTCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 249 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 400 GTAC GT^AC
566 GTAC
AocI 197 CCTTAGG CC^T[ACGT]AGG
1105 CCTCAGG
BsmAI 573 GAGAC GTCTC(1/5)
267 GTCTC
BsmFI 622 GTCCC GGGAC(10/14)
632 GTCCC
BsmI 249 GAATGC GAATGC(1/-1)
1174 GCATTC
BstAPI 244 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
684 GCAGGGTGTGC
Bsu36I 197 CCTTAGG CC^T[ACGT]AGG
1105 CCTCAGG
Cac8I 642 GCCAGC GC[ACGT]^[ACGT]GC
1125 GCAGGC
DsaI 272 CCATGG C^C[GA][CT]GG
819 CCATGG
Eco81I 197 CCTTAGG CC^T[ACGT]AGG
1105 CCTCAGG
MspA1I 925 CAGCGG C[AC]G^C[GT]G
1072 CAGCTG
NcoI 272 CCATGG C^CATGG
819 CCATGG
RsaI 400 GTAC GT^AC
566 GTAC
SacI 141 GAGCTC GAGCT^C
156 GAGCTC
SfaNI 802 GCATC GCATC(5/9)
1129 GCATC
TfiI 1017 GATTC G^A[AT]TC
1026 GAATC
Tsp509I 472 AATT ^AATT
862 AATT
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AspHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfcI StyI TseI
Tsp45I TspRI