(Circular) (Six-base) MAPSORT of: pFN21ASDA1737.seq Check: 3012 from: 1 to: 5926 pFN21ASDA1737 5926 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2720 2720 Size: 5926 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2735 3989 5373 5641 1632 Size: 1103 1254 1384 268 1917 Fragments arranged by size: 1917 1384 1254 1103 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 2451 1166 Size: 1285 4641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaI G_GGCC'C Cuts at: 3127 3127 Size: 5926 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2494 3235 3461 3613 5722 1493 Size: 1001 741 226 152 2109 1697 Fragments arranged by size: 2109 1697 1001 741 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 3251 1870 Size: 73 1308 4545 Fragments arranged by size: 4545 1308 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3247 4702 618 Size: 325 217 627 1460 1455 1842 Fragments arranged by size: 1842 1460 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2168 2183 3127 3245 1391 Size: 663 114 15 944 118 4072 Fragments arranged by size: 4072 944 663 118 114 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2065 2244 2607 928 Size: 971 166 179 363 4247 Fragments arranged by size: 4247 971 363 179 166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2770 3818 5028 2770 Size: 1048 1210 3668 Fragments arranged by size: 3668 1210 1048 BclI T'GATC_A Cuts at: 1548 3205 1548 Size: 1657 4269 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3131 4537 136 Size: 107 122 71 631 605 1459 1406 1525 Fragments arranged by size: 1525 1459 1406 631 605 122 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 2213 3343 2213 Size: 1130 4796 Bme1580I G_kGCm'C Cuts at: 1790 2718 3127 3987 5143 1790 Size: 928 409 860 1156 2573 Fragments arranged by size: 2573 1156 928 860 409 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2781 3657 4615 Size: 416 53 83 399 24 1364 876 958 Cuts at: 4615 442 Size: 1753 Fragments arranged by size: 1753 1364 958 876 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2875 2877 2875 Size: 2 5924 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2248 2333 2455 3032 4586 1239 Size: 1009 85 122 577 1554 2579 Fragments arranged by size: 2579 1554 1009 577 122 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2773 3223 3717 493 Size: 711 1569 450 494 2702 Fragments arranged by size: 2702 1569 711 494 450 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2663 3733 4115 4750 Size: 53 83 186 936 1130 1070 382 635 Cuts at: 4750 275 Size: 1451 Fragments arranged by size: 1451 1130 1070 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2275 2305 1178 Size: 30 165 30 872 30 4799 Fragments arranged by size: 4799 872 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5926 BseYI C'CCAG_C Cuts at: 2343 2675 3093 3211 5129 5625 2343 Size: 332 418 118 1918 496 2644 Fragments arranged by size: 2644 1918 496 418 332 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 1163 Size: 573 858 4495 Fragments arranged by size: 4495 858 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2168 2183 2916 3987 4072 5143 2168 Size: 15 733 1071 85 1071 2951 Fragments arranged by size: 2951 1071 1071 733 85 15 BsmI GAATG_Cn' Cuts at: 2278 3197 3437 3530 2278 Size: 919 240 93 4674 Fragments arranged by size: 4674 919 240 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2168 2183 2718 2916 3127 3245 Size: 399 264 114 15 535 198 211 118 Cuts at: 3245 3987 4072 5143 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 535 399 264 211 198 118 114 85 15 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 2304 3815 5545 2304 Size: 1511 1730 2685 Fragments arranged by size: 2685 1730 1511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2720 3281 844 Size: 1876 561 3489 Fragments arranged by size: 3489 1876 561 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2755 4570 4757 1700 Size: 110 945 1815 187 2869 Fragments arranged by size: 2869 1815 945 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2273 2713 794 Size: 1479 440 4007 Fragments arranged by size: 4007 1479 440 BstBI TT'CG_AA Cuts at: 2007 4730 2007 Size: 2723 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921 Size: 1284 367 385 17 1452 86 12 346 Cuts at: 5921 1972 Size: 1977 Fragments arranged by size: 1977 1452 1284 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2221 3129 2221 Size: 908 5018 BtgI C'CryG_G Cuts at: 513 1065 2022 2295 2842 513 Size: 552 957 273 547 3597 Fragments arranged by size: 3597 957 552 547 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3442 4236 4256 3190 Size: 252 794 20 4860 Fragments arranged by size: 4860 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3373 4262 5891 Size: 54 1425 50 275 18 1543 889 1629 Cuts at: 5891 8 Size: 43 Fragments arranged by size: 1629 1543 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2754 3856 1353 Size: 1401 1102 3423 Fragments arranged by size: 3423 1401 1102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2166 2181 2166 Size: 15 5911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2248 2333 2455 2735 3032 3989 4586 Size: 393 616 85 122 280 297 957 597 Cuts at: 4586 5373 5641 1239 Size: 787 268 1524 Fragments arranged by size: 1524 957 787 616 597 393 297 280 268 122 85 EcoO109I rG'GnC_Cy Cuts at: 2533 3123 3124 2533 Size: 590 1 5335 Fragments arranged by size: 5335 590 1 EcoRI G'AATT_C Cuts at: 2494 3235 3613 2494 Size: 741 378 4807 Fragments arranged by size: 4807 741 378 EcoRV GAT'ATC Cuts at: 2935 2935 Size: 5926 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2293 Size: 5926 FspI TGC'GCA Cuts at: 2824 4432 2824 Size: 1608 4318 HaeII r_GCGC'y Cuts at: 1678 2220 2682 2971 5073 1678 Size: 542 462 289 2102 2531 Fragments arranged by size: 2531 2102 542 462 289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2150 2161 2182 2447 Size: 32 135 32 557 21 11 21 265 Cuts at: 2447 2479 4567 4599 4641 4673 1373 Size: 32 2088 32 42 32 2626 Fragments arranged by size: 2626 2088 557 265 135 42 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 5040 5224 1305 Size: 1185 2550 184 2007 Fragments arranged by size: 2550 2007 1185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2451 2899 3884 4243 4402 518 Size: 648 924 361 448 985 359 159 2042 Fragments arranged by size: 2042 985 924 648 448 361 359 159 MspA1I CmG'CkG Cuts at: 1606 2950 3097 3342 4019 5167 5412 1606 Size: 1344 147 245 677 1148 245 2120 Fragments arranged by size: 2120 1344 1148 677 245 245 147 NcoI C'CATG_G Cuts at: 513 1065 2022 2295 2842 513 Size: 552 957 273 547 3597 Fragments arranged by size: 3597 957 552 547 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2212 3284 4829 1132 Size: 97 983 1072 1545 2229 Fragments arranged by size: 2229 1545 1072 983 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PfoI T'CCnGG_A Cuts at: 2471 2471 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PmlI CAC'GTG Cuts at: 2773 2773 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 3123 3123 Size: 5926 PstI C_TGCA'G Cuts at: 830 2188 2709 2911 3150 3278 830 Size: 1358 521 202 239 128 3478 Fragments arranged by size: 3478 1358 521 239 202 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 3097 3097 Size: 5926 SacI G_AGCT'C Cuts at: 2168 2183 2168 Size: 15 5911 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2184 2705 2907 3146 3274 4409 5090 Size: 219 1139 521 202 239 128 1135 681 Cuts at: 5090 5281 826 Size: 191 1471 Fragments arranged by size: 1471 1139 1135 681 521 239 219 202 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3223 3717 493 Size: 2730 494 2702 Fragments arranged by size: 2730 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2022 2295 2542 2631 2842 3030 3365 Size: 552 957 273 247 89 211 188 335 Cuts at: 3365 513 Size: 3074 Fragments arranged by size: 3074 957 552 335 273 247 211 188 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2689 3225 3953 3970 4123 4308 4753 Size: 1255 1109 536 728 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1498 1255 1109 728 536 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2588 4172 5884 Size: 274 80 33 51 1443 611 1584 1712 Cuts at: 5884 96 Size: 138 Fragments arranged by size: 1712 1584 1443 611 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2770 2770 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 2276 4055 2276 Size: 1779 4147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI