Restriction map for
pFN21ASDA1737
(Linear) (Six-base) MAPSORT of: KIAA1737.seq Check: 4671 from: 1 to: 1197
KIAA1737.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 1197
Size: 697 500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1197
Size: 712 485
AleI CACnn'nnGTG
Cuts at: 0 428 1197
Size: 428 769
ApaI G_GGCC'C
Cuts at: 0 1104 1197
Size: 1104 93
ApoI r'AATT_y
Cuts at: 0 471 1197
Size: 471 726
BanII G_rGCy'C
Cuts at: 0 31 145 160 1104 1197
Size: 31 114 15 944 93
Fragments arranged by size:
944 114 93 31 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 42 221 584 1197
Size: 42 179 363 613
Fragments arranged by size:
613 363 179 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 747 1197
Size: 747 450
BclI T'GATC_A
Cuts at: 0 1182 1197
Size: 1182 15
BglI GCCn_nnn'nGGC
Cuts at: 0 1108 1197
Size: 1108 89
BlpI GC'TnA_GC
Cuts at: 0 190 1197
Size: 190 1007
Bme1580I G_kGCm'C
Cuts at: 0 695 1104 1197
Size: 695 409 93
Fragments arranged by size:
695 409 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 758 1197
Size: 758 439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 854 1197
Size: 852 2 343
Fragments arranged by size:
852 343 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 310 432 1009 1197
Size: 225 85 122 577 188
Fragments arranged by size:
577 225 188 122 85
BsaAI yAC'GTr
Cuts at: 0 750 1197
Size: 750 447
BsaHI Gr'CG_yC
Cuts at: 0 640 1197
Size: 640 557
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 252 282 1197
Size: 252 30 915
Fragments arranged by size:
915 252 30
BseYI C'CCAG_C
Cuts at: 0 320 652 1070 1188 1197
Size: 320 332 418 118 9
Fragments arranged by size:
418 332 320 118 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1197
Size: 571 626
BsiHKAI G_wGCw'C
Cuts at: 0 145 160 893 1197
Size: 145 15 733 304
Fragments arranged by size:
733 304 145 15
BsmI GAATG_Cn'
Cuts at: 0 255 1174 1197
Size: 255 919 23
Fragments arranged by size:
919 255 23
Bsp1286I G_dGCh'C
Cuts at: 0 31 145 160 695 893 1104 1197
Size: 31 114 15 535 198 211 93
Fragments arranged by size:
535 211 198 114 93 31 15
BspHI T'CATG_A
Cuts at: 0 281 1197
Size: 281 916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 1197
Size: 697 500
BsrFI r'CCGG_y
Cuts at: 0 732 1197
Size: 732 465
BstAPI GCAn_nnn'nTGC
Cuts at: 0 250 690 1197
Size: 250 440 507
Fragments arranged by size:
507 440 250
Bsu36I CC'TnA_GG
Cuts at: 0 198 1106 1197
Size: 198 908 91
Fragments arranged by size:
908 198 91
BtgI C'CryG_G
Cuts at: 0 272 819 1197
Size: 272 547 378
Fragments arranged by size:
547 378 272
BtsI GCAGTG_nn'
Cuts at: 0 1167 1197
Size: 1167 30
EarI CTCTTCn'nnn_
Cuts at: 0 731 1197
Size: 731 466
EcoICRI GAG'CTC
Cuts at: 0 143 158 1197
Size: 143 15 1039
Fragments arranged by size:
1039 143 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 310 432 712 1009 1197
Size: 225 85 122 280 297 188
Fragments arranged by size:
297 280 225 188 122 85
EcoO109I rG'GnC_Cy
Cuts at: 0 510 1100 1101 1197
Size: 510 590 1 96
Fragments arranged by size:
590 510 96 1
EcoRI G'AATT_C
Cuts at: 0 471 1197
Size: 471 726
EcoRV GAT'ATC
Cuts at: 0 912 1197
Size: 912 285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 1197
Size: 270 927
FspI TGC'GCA
Cuts at: 0 801 1197
Size: 801 396
HaeII r_GCGC'y
Cuts at: 0 197 659 948 1197
Size: 197 462 289 249
Fragments arranged by size:
462 289 249 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 127 138 159 424 456 1197
Size: 106 21 11 21 265 32 741
Fragments arranged by size:
741 265 106 32 21 21 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1197
Size: 467 730
MslI CAynn'nnrTG
Cuts at: 0 67 428 876 1197
Size: 67 361 448 321
Fragments arranged by size:
448 361 321 67
MspA1I CmG'CkG
Cuts at: 0 927 1074 1197
Size: 927 147 123
Fragments arranged by size:
927 147 123
NcoI C'CATG_G
Cuts at: 0 272 819 1197
Size: 272 547 378
Fragments arranged by size:
547 378 272
NspI r_CATG'y
Cuts at: 0 189 1197
Size: 189 1008
PfoI T'CCnGG_A
Cuts at: 0 448 1197
Size: 448 749
PmlI CAC'GTG
Cuts at: 0 750 1197
Size: 750 447
PspOMI G'GGCC_C
Cuts at: 0 1100 1197
Size: 1100 97
PstI C_TGCA'G
Cuts at: 0 165 686 888 1127 1197
Size: 165 521 202 239 70
Fragments arranged by size:
521 239 202 165 70
PvuII CAG'CTG
Cuts at: 0 1074 1197
Size: 1074 123
SacI G_AGCT'C
Cuts at: 0 145 160 1197
Size: 145 15 1037
Fragments arranged by size:
1037 145 15
SfcI C'TryA_G
Cuts at: 0 161 682 884 1123 1197
Size: 161 521 202 239 74
Fragments arranged by size:
521 239 202 161 74
StyI C'CwwG_G
Cuts at: 0 272 519 608 819 1007 1197
Size: 272 247 89 211 188 190
Fragments arranged by size:
272 247 211 190 188 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 666 1197
Size: 666 531
TatI w'GTAC_w
Cuts at: 0 565 1197
Size: 565 632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 747 1197
Size: 747 450
XmnI GAAnn'nnTTC
Cuts at: 0 253 1197
Size: 253 944
Enzymes that do cut:
AarI AcuI AleI ApaI ApoI BanII BbsI BciVI
BclI BglI BlpI Bme1580I BmrI BplI BpmI BsaAI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrFI BstAPI Bsu36I BtgI BtsI EarI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
MmeI MslI MspA1I NcoI NspI PfoI PmlI PspOMI
PstI PvuII SacI SfcI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaBI
BsaWI BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI