(Circular) (Six-base) MAPSORT of: pFN21ASDA1735.seq Check: 8918 from: 1 to: 6145 pFN21ASDA1735 6145 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2157 2355 2531 2675 2883 2953 4208 5592 Size: 525 198 176 144 208 70 1255 1384 Cuts at: 5592 5860 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1255 525 268 208 198 176 144 70 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 3213 3309 4976 3213 Size: 96 1667 4382 Fragments arranged by size: 4382 1667 96 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 3186 3888 5460 3186 Size: 702 1572 3871 Fragments arranged by size: 3871 1572 702 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2181 2384 3470 1870 Size: 73 238 203 1086 4545 Fragments arranged by size: 4545 1086 238 203 73 BamHI G'GATC_C Cuts at: 2324 3331 3475 3842 2324 Size: 1007 144 367 4627 Fragments arranged by size: 4627 1007 367 144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3466 4921 618 Size: 325 217 627 1679 1455 1842 Fragments arranged by size: 1842 1679 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2204 3167 3464 1391 Size: 813 963 297 4072 Fragments arranged by size: 4072 963 813 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3429 928 Size: 971 1530 3644 Fragments arranged by size: 3644 1530 971 BbvCI CC'TCA_GC Cuts at: 2985 2985 Size: 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2663 2697 2918 2952 4336 4370 1903 Size: 34 726 34 221 34 1384 34 3678 Fragments arranged by size: 3678 1384 726 221 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 4206 5362 1790 Size: 2416 1156 2573 Fragments arranged by size: 2573 2416 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 2092 3876 Size: 416 53 83 399 24 635 40 1784 Cuts at: 3876 4834 442 Size: 958 1753 Fragments arranged by size: 1784 1753 958 635 416 399 83 53 40 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2541 2963 2541 Size: 422 5723 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 4805 1239 Size: 998 2568 2579 Fragments arranged by size: 2579 2568 998 Bpu10I CC'TnA_GC Cuts at: 2985 2985 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3087 4270 5135 5433 3087 Size: 1183 865 298 3799 Fragments arranged by size: 3799 1183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 4808 882 Size: 617 714 2595 2219 Fragments arranged by size: 2595 2219 714 617 BsaAI yAC'GTr Cuts at: 493 1204 3442 3936 493 Size: 711 2238 494 2702 Fragments arranged by size: 2702 2238 711 494 BsaBI GATnn'nnATC Cuts at: 2402 6139 2402 Size: 3737 2408 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3952 4334 4969 275 Size: 53 83 186 936 2419 382 635 1451 Fragments arranged by size: 2419 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2724 3213 3309 4578 4976 5250 5397 5808 2724 Size: 489 96 1269 398 274 147 411 3061 Fragments arranged by size: 3061 1269 489 411 398 274 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2155 2185 3251 3281 1178 Size: 30 165 30 752 30 1066 30 4042 Fragments arranged by size: 4042 1066 752 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 3272 3371 1186 Size: 1261 825 99 3960 Fragments arranged by size: 3960 1261 825 99 BseYI C'CCAG_C Cuts at: 2668 5348 5844 2668 Size: 2680 496 2969 Fragments arranged by size: 2969 2680 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 1163 Size: 573 843 4729 Fragments arranged by size: 4729 843 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3167 3201 4206 4291 5362 3167 Size: 34 1005 85 1071 3950 Fragments arranged by size: 3950 1071 1005 85 34 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 BsmBI CGTCTCn'nnnn_ Cuts at: 3047 3047 Size: 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 3167 3201 3464 4206 4291 5362 Size: 399 414 963 34 263 742 85 1071 Cuts at: 5362 1391 Size: 2174 Fragments arranged by size: 2174 1071 963 742 414 399 263 85 34 BspEI T'CCGG_A Cuts at: 2724 5808 2724 Size: 3084 3061 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2934 3500 844 Size: 2090 566 3489 Fragments arranged by size: 3489 2090 566 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2866 4640 4814 65 Size: 1183 1618 1774 174 1396 Fragments arranged by size: 1774 1618 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2640 3213 3309 4789 4976 1700 Size: 110 830 573 96 1480 187 2869 Fragments arranged by size: 2869 1480 830 573 187 110 96 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 2007 4949 2007 Size: 2942 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2324 2611 2758 3331 3475 3842 4227 4244 Size: 352 287 147 573 144 367 385 17 Cuts at: 4244 5696 5782 5794 6140 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 573 385 367 352 346 287 147 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3427 513 Size: 552 957 1405 3231 Fragments arranged by size: 3231 1405 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 3661 4455 4475 3661 Size: 794 20 5331 Fragments arranged by size: 5331 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3280 3449 3280 Size: 169 5976 DraIII CAC_nnn'GTG Cuts at: 2755 2755 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2192 2974 3592 Size: 54 1425 50 275 18 362 782 618 Cuts at: 3592 4481 6110 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 782 618 362 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 4075 1353 Size: 747 1975 3423 Fragments arranged by size: 3423 1975 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3165 3165 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2237 2355 2531 2675 2883 2953 Size: 393 525 80 118 176 144 208 70 Cuts at: 2953 4208 4805 5592 5860 1239 Size: 1255 597 787 268 1524 Fragments arranged by size: 1524 1255 787 597 525 393 268 208 176 144 118 80 70 EcoO109I rG'GnC_Cy Cuts at: 2352 2352 Size: 6145 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 EcoRV GAT'ATC Cuts at: 2295 2295 Size: 6145 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2524 2524 Size: 6145 FspI TGC'GCA Cuts at: 2960 4651 2960 Size: 1691 4454 HaeII r_GCGC'y Cuts at: 1678 3040 5292 1678 Size: 1362 2252 2531 Fragments arranged by size: 2531 2252 1362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 4786 4818 4860 Size: 32 135 32 583 32 2599 32 42 Cuts at: 4860 4892 1373 Size: 32 2626 Fragments arranged by size: 2626 2599 583 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 2900 3194 3234 5259 5443 1305 Size: 918 677 294 40 2025 184 2007 Fragments arranged by size: 2025 2007 918 677 294 184 40 MscI TGG'CCA Cuts at: 10 64 1489 1832 2976 10 Size: 54 1425 343 1144 3179 Fragments arranged by size: 3179 1425 1144 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2784 3372 4103 4462 4621 518 Size: 648 1618 588 731 359 159 2042 Fragments arranged by size: 2042 1618 731 648 588 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 2239 3561 4238 5386 5631 1606 Size: 529 104 1322 677 1148 245 2120 Fragments arranged by size: 2120 1322 1148 677 529 245 104 NcoI C'CATG_G Cuts at: 513 1065 2022 3427 513 Size: 552 957 1405 3231 Fragments arranged by size: 3231 1405 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3047 3503 5048 1132 Size: 97 1818 456 1545 2229 Fragments arranged by size: 2229 1818 1545 456 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2608 2755 1108 Size: 1500 147 4498 Fragments arranged by size: 4498 1500 147 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2036 1613 Size: 423 5722 PsiI TTA'TAA Cuts at: 2093 2402 3715 2093 Size: 309 1313 4523 Fragments arranged by size: 4523 1313 309 PstI C_TGCA'G Cuts at: 830 2619 2931 3497 830 Size: 1789 312 566 3478 Fragments arranged by size: 3478 1789 566 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2239 2239 Size: 6145 SacI G_AGCT'C Cuts at: 3167 3167 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2371 2751 2371 Size: 380 5765 SfcI C'TryA_G Cuts at: 826 1045 2303 2615 2927 3493 4628 5309 5500 Size: 219 1258 312 312 566 1135 681 191 Cuts at: 5500 826 Size: 1471 Fragments arranged by size: 1471 1258 1135 681 566 312 312 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2181 3102 4249 5150 5412 820 Size: 197 926 238 921 1147 901 262 1553 Fragments arranged by size: 1553 1147 926 921 901 262 238 197 SnaBI TAC'GTA Cuts at: 493 3442 3936 493 Size: 2949 494 2702 Fragments arranged by size: 2949 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 2258 4069 5 Size: 47 2206 1811 2081 Fragments arranged by size: 2206 2081 1811 47 StuI AGG'CCT Cuts at: 1517 2309 1517 Size: 792 5353 StyI C'CwwG_G Cuts at: 513 1065 2022 2355 3427 3584 513 Size: 552 957 333 1072 157 3074 Fragments arranged by size: 3074 1072 957 552 333 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2071 3109 3403 4172 4189 4342 4527 Size: 1255 491 1038 294 769 17 153 185 Cuts at: 4527 4972 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1038 769 491 445 294 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3188 4391 6103 Size: 274 80 33 51 1443 1211 1203 1712 Cuts at: 6103 96 Size: 138 Fragments arranged by size: 1712 1443 1211 1203 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6105 6137 6105 Size: 32 6113 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 2761 3481 2761 Size: 720 5425 XhoI C'TCGA_G Cuts at: 1943 2181 1943 Size: 238 5907 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI