(Circular) (Six-base) MAPSORT of: pFN21ASDA1721.seq Check: 7811 from: 1 to: 8182 pFN21ASDA1721 8182 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5992 278 Size: 53 83 186 936 4456 2468 Fragments arranged by size: 4456 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3216 3499 5525 1937 Size: 1279 283 2026 4594 Fragments arranged by size: 4594 2026 1279 283 Acc65I G'GTAC_C Cuts at: 5503 5503 Size: 8182 AclI AA'CG_TT Cuts at: 1563 6309 6682 1563 Size: 4746 373 3063 Fragments arranged by size: 4746 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2667 3060 3765 4334 4343 6245 7629 7897 Size: 1035 393 705 569 9 1902 1384 268 Cuts at: 7897 1632 Size: 1917 Fragments arranged by size: 1917 1902 1384 1035 705 569 393 268 9 AflII C'TTAA_G Cuts at: 820 1017 4372 5183 820 Size: 197 3355 811 3819 Fragments arranged by size: 3819 3355 811 197 AflIII A'CryG_T Cuts at: 1203 5259 7081 8058 1203 Size: 4056 1822 977 1327 Fragments arranged by size: 4056 1822 1327 977 AgeI A'CCGG_T Cuts at: 7013 7013 Size: 8182 AhdI GACnn_n'nnGTC Cuts at: 6911 6911 Size: 8182 AleI CACnn'nnGTG Cuts at: 1166 4151 5264 1166 Size: 2985 1113 4084 Fragments arranged by size: 4084 2985 1113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2934 4134 1184 Size: 1750 1200 5232 Fragments arranged by size: 5232 1750 1200 AlwNI CAG_nnn'CTG Cuts at: 3830 4574 5925 7497 3830 Size: 744 1351 1572 4515 Fragments arranged by size: 4515 1572 1351 744 ApaI G_GGCC'C Cuts at: 2046 2046 Size: 8182 ApaLI G'TGCA_C Cuts at: 6239 7395 6239 Size: 1156 7026 ApoI r'AATT_y Cuts at: 1493 2519 2564 2746 3367 3771 4204 4796 5438 Size: 1026 45 182 621 404 433 592 642 Cuts at: 5438 5491 5717 5869 7978 1493 Size: 53 226 152 2109 1697 Fragments arranged by size: 2109 1697 1026 642 621 592 433 404 226 182 152 53 45 AseI AT'TA_AT Cuts at: 160 1032 3756 4970 6736 160 Size: 872 2724 1214 1766 1606 Fragments arranged by size: 2724 1766 1606 1214 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8182 AvaI C'yCGr_G Cuts at: 1870 1943 3879 5507 1870 Size: 73 1936 1628 4545 Fragments arranged by size: 4545 1936 1628 73 BamHI G'GATC_C Cuts at: 5512 5879 5512 Size: 367 7815 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 5503 6958 618 Size: 325 217 627 305 3411 1455 1842 Fragments arranged by size: 3411 1842 1455 627 325 305 217 BanII G_rGCy'C Cuts at: 1391 2046 5501 1391 Size: 655 3455 4072 Fragments arranged by size: 4072 3455 655 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2607 2673 928 Size: 971 708 66 6437 Fragments arranged by size: 6437 971 708 66 BbvCI CC'TCA_GC Cuts at: 4470 4470 Size: 8182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6373 6407 1903 Size: 34 4436 34 3678 Fragments arranged by size: 4436 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6074 7284 6074 Size: 1210 6972 BclI T'GATC_A Cuts at: 1548 3448 3994 1548 Size: 1900 546 5736 Fragments arranged by size: 5736 1900 546 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6793 136 Size: 107 122 71 631 605 5121 1525 Fragments arranged by size: 5121 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2613 3895 4862 8177 2613 Size: 1282 967 3315 2618 Fragments arranged by size: 3315 2618 1282 967 BlpI GC'TnA_GC Cuts at: 2061 4212 5599 2061 Size: 2151 1387 4644 Fragments arranged by size: 4644 2151 1387 Bme1580I G_kGCm'C Cuts at: 1790 2046 6243 7399 1790 Size: 256 4197 1156 2573 Fragments arranged by size: 4197 2573 1156 256 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8182 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2917 5324 5913 Size: 416 53 83 399 24 1500 2407 589 Cuts at: 5913 6871 442 Size: 958 1753 Fragments arranged by size: 2407 1753 1500 958 589 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8182 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3914 Size: 8182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 3040 3336 3693 3768 4120 5120 6842 Size: 848 953 296 357 75 352 1000 1722 Cuts at: 6842 1239 Size: 2579 Fragments arranged by size: 2579 1722 1000 953 848 357 352 296 75 Bpu10I CC'TnA_GC Cuts at: 4470 4470 Size: 8182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2743 6307 7172 7470 2743 Size: 3564 865 298 3455 Fragments arranged by size: 3564 3455 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3429 5465 6845 882 Size: 617 1930 2036 1380 2219 Fragments arranged by size: 2219 2036 1930 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 5479 5973 493 Size: 711 4275 494 2702 Fragments arranged by size: 4275 2702 711 494 BsaBI GATnn'nnATC Cuts at: 8176 8176 Size: 8182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5989 6371 7006 275 Size: 53 83 186 936 4456 382 635 1451 Fragments arranged by size: 4456 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6615 7013 7287 7434 7845 6615 Size: 398 274 147 411 6952 Fragments arranged by size: 6952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3236 3266 1178 Size: 30 165 30 1833 30 6094 Fragments arranged by size: 6094 1833 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3118 3413 5376 1186 Size: 1932 295 1963 3992 Fragments arranged by size: 3992 1963 1932 295 BseYI C'CCAG_C Cuts at: 2038 3954 7385 7881 2038 Size: 1916 3431 496 2339 Fragments arranged by size: 3431 2339 1916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4154 4283 1163 Size: 573 2418 129 5062 Fragments arranged by size: 5062 2418 573 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5632 6393 7421 1540 Size: 275 186 3631 761 1028 2301 Fragments arranged by size: 3631 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 6243 6328 7399 2139 Size: 4104 85 1071 2922 Fragments arranged by size: 4104 2922 1071 85 BsmI GAATG_Cn' Cuts at: 3474 3736 5693 5786 3474 Size: 262 1957 93 5870 Fragments arranged by size: 5870 1957 262 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2139 5501 6243 6328 7399 1391 Size: 399 256 93 3362 742 85 1071 2174 Fragments arranged by size: 3362 2174 1071 742 399 256 93 85 BspEI T'CCGG_A Cuts at: 7845 7845 Size: 8182 BspHI T'CATG_A Cuts at: 2437 6071 7801 2437 Size: 3634 1730 2818 Fragments arranged by size: 3634 2818 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4761 4791 5537 844 Size: 3917 30 746 3489 Fragments arranged by size: 3917 3489 746 30 BsrBI CCG'CTC Cuts at: 1394 1823 6069 1394 Size: 429 4246 3507 Fragments arranged by size: 4246 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4464 6677 6851 65 Size: 1183 3216 2213 174 1396 Fragments arranged by size: 3216 2213 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6826 7013 1700 Size: 110 5016 187 2869 Fragments arranged by size: 5016 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8182 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8182 BssSI C'ACGA_G Cuts at: 3393 6242 7254 3393 Size: 2849 1012 4321 Fragments arranged by size: 4321 2849 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2791 794 Size: 1997 6185 BstBI TT'CG_AA Cuts at: 2007 6986 2007 Size: 4979 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8182 BstXI CCAn_nnnn'nTGG Cuts at: 3385 3385 Size: 8182 BstYI r'GATC_y Cuts at: 1972 2613 3051 3779 3895 4862 4927 5023 5512 Size: 641 438 728 116 967 65 96 489 Cuts at: 5512 5879 6264 6281 7733 7819 7831 8177 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 967 728 641 489 438 385 367 346 116 96 86 65 17 12 BstZ17I GTA'TAC Cuts at: 3217 3500 3217 Size: 283 7899 Bsu36I CC'TnA_GG Cuts at: 5019 5019 Size: 8182 BtgI C'CryG_G Cuts at: 513 1065 2103 2569 3544 513 Size: 552 1038 466 975 5151 Fragments arranged by size: 5151 1038 975 552 466 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7443 Fragments arranged by size: 7443 683 56 BtsI GCAGTG_nn' Cuts at: 2918 4437 5235 5370 5698 6492 6512 2918 Size: 1519 798 135 328 794 20 4588 Fragments arranged by size: 4588 1519 798 794 328 135 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8147 DraI TTT'AAA Cuts at: 3948 5032 5486 3948 Size: 1084 454 6644 Fragments arranged by size: 6644 1084 454 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3546 7189 809 Size: 507 2230 3643 1802 Fragments arranged by size: 3643 2230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3456 5629 6518 Size: 54 1425 50 275 18 1626 2173 889 Cuts at: 6518 8147 8 Size: 1629 43 Fragments arranged by size: 2173 1629 1626 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5629 1537 Size: 275 3817 4090 Fragments arranged by size: 4090 3817 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2832 2940 3510 4156 4731 6112 1353 Size: 1479 108 570 646 575 1381 3423 Fragments arranged by size: 3423 1479 1381 646 575 570 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6769 7143 7289 387 Size: 1064 138 5180 374 146 1280 Fragments arranged by size: 5180 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2667 3040 3060 3336 3693 3765 Size: 393 455 580 373 20 276 357 72 Cuts at: 3765 3768 4120 4334 4343 5120 6245 6842 7629 Size: 3 352 214 9 777 1125 597 787 Cuts at: 7629 7897 1239 Size: 268 1524 Fragments arranged by size: 1524 1125 787 777 597 580 455 393 373 357 352 276 268 214 72 20 9 3 EcoO109I rG'GnC_Cy Cuts at: 2042 2043 4114 4453 2042 Size: 1 2071 339 5771 Fragments arranged by size: 5771 2071 339 1 EcoRI G'AATT_C Cuts at: 2564 4796 5491 5869 2564 Size: 2232 695 378 4877 Fragments arranged by size: 4877 2232 695 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2621 3212 4685 4772 5434 2621 Size: 591 1473 87 662 5369 Fragments arranged by size: 5369 1473 662 591 87 FspI TGC'GCA Cuts at: 6688 6688 Size: 8182 HaeII r_GCGC'y Cuts at: 1678 2039 7329 1678 Size: 361 5290 2531 Fragments arranged by size: 5290 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2948 2980 4267 4299 4564 Size: 32 135 32 1376 32 1287 32 265 Cuts at: 4564 4596 4612 4644 6823 6855 6897 6929 1373 Size: 32 16 32 2179 32 42 32 2626 Fragments arranged by size: 2626 2179 1376 1287 265 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2625 3139 3255 3604 3751 5526 Size: 988 281 687 514 116 349 147 1775 Cuts at: 5526 5772 669 Size: 246 3079 Fragments arranged by size: 3079 1775 988 687 514 349 281 246 147 116 HindIII A'AGCT_T Cuts at: 748 5250 748 Size: 4502 3680 HpaI GTT'AAC Cuts at: 2625 3139 3255 5772 2625 Size: 514 116 2517 5035 Fragments arranged by size: 5035 2517 514 116 KpnI G_GTAC'C Cuts at: 5507 5507 Size: 8182 MfeI C'AATT_G Cuts at: 2415 4531 5781 2415 Size: 2116 1250 4816 Fragments arranged by size: 4816 2116 1250 MluI A'CGCG_T Cuts at: 8058 8058 Size: 8182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3222 4967 7296 7480 1305 Size: 1917 1745 2329 184 2007 Fragments arranged by size: 2329 2007 1917 1745 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3458 10 Size: 54 1425 343 1626 4734 Fragments arranged by size: 4734 1626 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4151 5228 5264 6140 6499 6658 518 Size: 648 2985 1077 36 876 359 159 2042 Fragments arranged by size: 2985 2042 1077 876 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2065 4877 5598 6275 7423 7668 1606 Size: 459 2812 721 677 1148 245 2120 Fragments arranged by size: 2812 2120 1148 721 677 459 245 NcoI C'CATG_G Cuts at: 513 1065 2103 2569 3544 513 Size: 552 1038 466 975 5151 Fragments arranged by size: 5151 1038 975 552 466 NdeI CA'TA_TG Cuts at: 387 3229 4308 387 Size: 2842 1079 4261 Fragments arranged by size: 4261 2842 1079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8182 NotI GC'GGCC_GC Cuts at: 5629 5629 Size: 8182 NspI r_CATG'y Cuts at: 1132 1229 2132 3308 5540 7085 1132 Size: 97 903 1176 2232 1545 2229 Fragments arranged by size: 2232 2229 1545 1176 903 97 PasI CC'CwG_GG Cuts at: 1348 3809 1348 Size: 2461 5721 PciI A'CATG_T Cuts at: 7081 7081 Size: 8182 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8182 PfoI T'CCnGG_A Cuts at: 3784 3784 Size: 8182 PmeI GTTT'AAAC Cuts at: 5486 5486 Size: 8182 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6251 6283 7764 7796 6251 Size: 32 1481 32 6637 Fragments arranged by size: 6637 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4114 4114 Size: 8182 PshAI GACnn'nnGTC Cuts at: 1613 2607 1613 Size: 994 7188 PsiI TTA'TAA Cuts at: 5752 5752 Size: 8182 PspOMI G'GGCC_C Cuts at: 2042 2042 Size: 8182 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2352 2384 3483 3515 2352 Size: 32 1099 32 7019 Fragments arranged by size: 7019 1099 32 32 PstI C_TGCA'G Cuts at: 830 2643 2913 3490 5534 830 Size: 1813 270 577 2044 3478 Fragments arranged by size: 3478 2044 1813 577 270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8182 PvuII CAG'CTG Cuts at: 2065 2065 Size: 8182 SalI G'TCGA_C Cuts at: 1936 5524 1936 Size: 3588 4594 SapI GCTCTTCn'nnn_ Cuts at: 4731 4731 Size: 8182 SbfI CC_TGCA'GG Cuts at: 5534 5534 Size: 8182 ScaI AGT'ACT Cuts at: 4961 6430 8142 4961 Size: 1469 1712 5001 Fragments arranged by size: 5001 1712 1469 SfcI C'TryA_G Cuts at: 826 1045 2639 2909 3486 5530 6665 7346 7537 Size: 219 1594 270 577 2044 1135 681 191 Cuts at: 7537 826 Size: 1471 Fragments arranged by size: 2044 1594 1471 1135 681 577 270 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8182 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8182 SmaI CCC'GGG Cuts at: 1872 5509 1872 Size: 3637 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2722 3879 4372 5183 6286 7187 Size: 197 926 779 1157 493 811 1103 901 Cuts at: 7187 7449 820 Size: 262 1553 Fragments arranged by size: 1553 1157 1103 926 901 811 779 493 262 197 SnaBI TAC'GTA Cuts at: 493 5479 5973 493 Size: 4986 494 2702 Fragments arranged by size: 4986 2702 494 SpeI A'CTAG_T Cuts at: 152 2201 152 Size: 2049 6133 SphI G_CATG'C Cuts at: 1132 2132 5540 1132 Size: 1000 3408 3774 Fragments arranged by size: 3774 3408 1000 SspI AAT'ATT Cuts at: 5 52 3725 5288 6106 5 Size: 47 3673 1563 818 2081 Fragments arranged by size: 3673 2081 1563 818 47 StuI AGG'CCT Cuts at: 1517 5105 1517 Size: 3588 4594 StyI C'CwwG_G Cuts at: 513 1065 2103 2569 3544 5621 513 Size: 552 1038 466 975 2077 3074 Fragments arranged by size: 3074 2077 1038 975 552 466 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6209 6226 6379 6564 7009 325 Size: 1255 4629 17 153 185 445 1498 Fragments arranged by size: 4629 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2863 4016 4959 Size: 274 80 33 51 1443 886 1153 943 Cuts at: 4959 5043 6428 8140 96 Size: 84 1385 1712 138 Fragments arranged by size: 1712 1443 1385 1153 943 886 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8142 8174 8142 Size: 32 8150 Tth111I GACn'n_nGTC Cuts at: 1316 2216 1316 Size: 900 7282 XbaI T'CTAG_A Cuts at: 5518 5518 Size: 8182 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2811 2242 Size: 569 7613 XhoI C'TCGA_G Cuts at: 1943 3879 1943 Size: 1936 6246 XmaI C'CCGG_G Cuts at: 1870 5507 1870 Size: 3637 4545 XmnI GAAnn'nnTTC Cuts at: 2364 2604 3636 3826 6311 2364 Size: 240 1032 190 2485 4235 Fragments arranged by size: 4235 2485 1032 240 190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5990 276 Size: 53 83 186 936 4456 2468 Fragments arranged by size: 4456 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI