Restriction map for
pFN21ASDA1720
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BalI
BcgI BciVI BclI BfrI BglI
BglII BsaBI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MluNI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 644 TCCGGA T^CCGGA
AfaI 408 GTAC GT^AC
AlwNI 700 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 399 AGCGCT AGC^GCT
AspI 1075 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1013 TGCGCA TGC^GCA
AvrII 82 CCTAGG C^CTAGG
BbrPI 501 CACGTG CAC^GTG
BbsI 636 GAAGAC GAAGAC(2/6)
BbvCI 610 GCTGAGG CCTCAGC(-5/-2)
BlnI 82 CCTAGG C^CTAGG
BpuAI 636 GAAGAC GAAGAC(2/6)
BsaAI 501 CACGTG [CT]AC^GT[GA]
BsaI 34 GAGACC GGTCTC(1/5)
BsaWI 644 TCCGGA [AT]^CCGG[AT]
BseAI 644 TCCGGA T^CCGGA
BspEI 644 TCCGGA T^CCGGA
BstAPI 507 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 1092 CGGCCA [CT]^GGCC[GA]
Eco47III 399 AGCGCT AGC^GCT
FspI 1013 TGCGCA TGC^GCA
KspI 825 CCGCGG CCGC^GG
MroI 644 TCCGGA T^CCGGA
NspI 425 GCATGC [GA]CATG^[CT]
PaeR7I 440 CTCGAG C^TCGAG
PflFI 1075 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 501 CACGTG CAC^GTG
PmlI 501 CACGTG CAC^GTG
PpuMI 888 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 238 CAGCTG CAG^CTG
RsaI 408 GTAC GT^AC
SacII 825 CCGCGG CCGC^GG
SexAI 224 ACCAGGT A^CC[AT]GGT
SphI 425 GCATGC GCATG^C
StuI 1065 AGGCCT AGG^CCT
TfiI 810 GATTC G^A[AT]TC
Tsp509I 140 AATT ^AATT
TspRI 129 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1075 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 440 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 852 GGGCCC GGGCC^C
967 GGGCCC
BamHI 168 GGATCC G^GATCC
269 GGATCC
BbeI 821 GGCGCC GGCGC^C
1121 GGCGCC
BfaI 83 CTAG C^TAG
173 CTAG
BsmFI 79 GTCCC GGGAC(10/14)
144 GTCCC
BstF5I 72 GGATG GGATG(2/0)
243 GGATG
EarI 100 GAAGAG CTCTTC(1/4)
553 GAAGAG
EcoT14I 82 CCTAGG C^C[AT][AT]GG
722 CCAAGG
FokI 72 GGATG GGATG(9/13)
243 GGATG
KasI 821 GGCGCC G^GCGCC
1121 GGCGCC
Ksp632I 100 GAAGAG CTCTTC(1/4)
553 GAAGAG
MaeI 83 CTAG C^TAG
173 CTAG
MaeII 502 ACGT A^CGT
1079 ACGT
NarI 821 GGCGCC GG^CGCC
1121 GGCGCC
PstI 631 CTGCAG CTGCA^G
1033 CTGCAG
SacI 7 GAGCTC GAGCT^C
679 GAGCTC
SapI 100 GAAGAGC GCTCTTC(1/4)
553 GAAGAGC
SfaNI 513 GATGC GCATC(5/9)
326 GCATC
SfoI 821 GGCGCC GGC^GCC
1121 GGCGCC
SmlI 440 CTCGAG C^T[CT][GA]AG
682 CTCAAG
StyI 82 CCTAGG C^C[AT][AT]GG
722 CCAAGG
Tsp45I 589 GTGAC ^GT[GC]AC
727 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoNI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
SfcI TaqI TseI TthHB8I Van91I
XhoII