(Circular) (Six-base) MAPSORT of: pFN21ASDA1720.seq Check: 5529 from: 1 to: 5908 pFN21ASDA1720 5908 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2103 3033 3971 5355 5623 1632 Size: 471 930 938 1384 268 1917 Fragments arranged by size: 1917 1384 938 930 471 268 AfeI AGC'GCT Cuts at: 2424 2424 Size: 5908 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2475 1184 Size: 1291 4617 AlwNI CAG_nnn'CTG Cuts at: 2728 3651 5223 2728 Size: 923 1572 3413 Fragments arranged by size: 3413 1572 923 ApaI G_GGCC'C Cuts at: 2879 2994 2879 Size: 115 5793 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 3217 3443 3595 5704 1493 Size: 1724 226 152 2109 1697 Fragments arranged by size: 2109 1724 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2463 2640 2915 3233 1870 Size: 73 520 177 275 318 4545 Fragments arranged by size: 4545 520 318 275 177 73 AvrII C'CTAG_G Cuts at: 2105 2105 Size: 5908 BamHI G'GATC_C Cuts at: 2191 2292 3238 3605 2191 Size: 101 946 367 4494 Fragments arranged by size: 4494 946 367 101 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2844 3144 3180 3229 4684 Size: 325 217 627 1057 300 36 49 1455 Cuts at: 4684 618 Size: 1842 Fragments arranged by size: 1842 1455 1057 627 325 300 217 49 36 BanII G_rGCy'C Cuts at: 1391 2034 2114 2706 2879 2994 3169 3227 1391 Size: 643 80 592 173 115 175 58 4072 Fragments arranged by size: 4072 643 592 175 173 115 80 58 BbeI G_GCGC'C Cuts at: 2848 3148 2848 Size: 300 5608 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2666 928 Size: 971 767 4170 Fragments arranged by size: 4170 971 767 BbvCI CC'TCA_GC Cuts at: 2634 2634 Size: 5908 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 2028 2400 2484 3029 3325 2028 Size: 372 84 545 296 4611 Fragments arranged by size: 4611 545 372 296 84 Bme1580I G_kGCm'C Cuts at: 1790 2879 2994 3969 5125 1790 Size: 1089 115 975 1156 2573 Fragments arranged by size: 2573 1156 1089 975 115 BmgBI CAC'GTC Cuts at: 1318 3103 1318 Size: 1785 4123 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 2707 3639 Size: 416 53 83 399 24 691 599 932 Cuts at: 3639 4597 442 Size: 958 1753 Fragments arranged by size: 1753 958 932 691 599 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 2750 2780 2834 2990 4568 1239 Size: 912 599 30 54 156 1578 2579 Fragments arranged by size: 2579 1578 912 599 156 54 30 Bpu10I CC'TnA_GC Cuts at: 2391 2634 2775 2391 Size: 243 141 5524 Fragments arranged by size: 5524 243 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 4033 4898 5196 2690 Size: 1343 865 298 3402 Fragments arranged by size: 3402 1343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 4571 882 Size: 617 552 2520 2219 Fragments arranged by size: 2520 2219 617 552 BsaAI yAC'GTr Cuts at: 493 1204 2526 3205 3699 493 Size: 711 1322 679 494 2702 Fragments arranged by size: 2702 1322 711 679 494 BsaBI GATnn'nnATC Cuts at: 5902 5902 Size: 5908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 2845 3145 3715 Size: 53 83 186 936 960 352 300 570 Cuts at: 3715 4097 4732 275 Size: 382 635 1451 Fragments arranged by size: 1451 960 936 635 570 382 352 300 186 83 53 BsaWI w'CCGG_w Cuts at: 2667 4341 4739 5013 5160 5571 2667 Size: 1674 398 274 147 411 3004 Fragments arranged by size: 3004 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5683 Fragments arranged by size: 5683 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 2192 2222 2252 2747 2878 2987 1186 Size: 962 44 30 30 495 131 109 4107 Fragments arranged by size: 4107 962 495 131 109 44 30 30 BseYI C'CCAG_C Cuts at: 3124 5111 5607 3124 Size: 1987 496 3425 Fragments arranged by size: 3425 1987 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3358 4119 5147 1540 Size: 275 186 1357 761 1028 2301 Fragments arranged by size: 2301 1357 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2220 2604 2706 2739 3081 3969 4054 5125 Size: 186 384 102 33 342 888 85 1071 Cuts at: 5125 2034 Size: 2817 Fragments arranged by size: 2817 1071 888 384 342 186 102 85 33 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2114 2220 2604 2706 2739 2879 Size: 399 244 80 106 384 102 33 140 Cuts at: 2879 2994 3081 3169 3227 3969 4054 5125 1391 Size: 115 87 88 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 384 244 140 115 106 102 88 87 85 80 58 33 BspEI T'CCGG_A Cuts at: 2667 5571 2667 Size: 2904 3004 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 2758 3063 3263 844 Size: 1717 197 305 200 3489 Fragments arranged by size: 3489 1717 305 200 197 BsrBI CCG'CTC Cuts at: 1394 1823 2437 2581 2596 3795 1394 Size: 429 614 144 15 1199 3507 Fragments arranged by size: 3507 1199 614 429 144 15 BsrDI GCAATG_nn' Cuts at: 65 1248 4403 4577 65 Size: 1183 3155 174 1396 Fragments arranged by size: 3155 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4552 4739 1700 Size: 110 2742 187 2869 Fragments arranged by size: 2869 2742 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5908 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 2536 794 Size: 1742 4166 BstBI TT'CG_AA Cuts at: 2007 4712 2007 Size: 2705 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 2191 2292 2724 3238 3605 3990 4007 5459 Size: 219 101 432 514 367 385 17 1452 Cuts at: 5459 5545 5557 5903 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 514 432 385 367 346 219 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2796 2848 2895 3072 3169 513 Size: 552 957 774 52 47 177 97 3252 Fragments arranged by size: 3252 957 774 552 177 97 52 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 2158 3424 4218 4238 2158 Size: 1266 794 20 3828 Fragments arranged by size: 3828 1266 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3115 3355 4244 Size: 54 1425 50 275 18 1285 240 889 Cuts at: 4244 5873 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1285 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2571 3838 1353 Size: 765 453 1267 3423 Fragments arranged by size: 3423 1267 765 453 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2585 3177 4495 4869 5015 387 Size: 1064 138 996 592 1318 374 146 1280 Fragments arranged by size: 1318 1280 1064 996 592 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2704 2032 Size: 672 5236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2151 2750 2780 2834 2990 3033 Size: 393 471 48 599 30 54 156 43 Cuts at: 3033 3971 4568 5355 5623 1239 Size: 938 597 787 268 1524 Fragments arranged by size: 1524 938 787 599 597 471 393 268 156 54 48 43 30 EcoNI CCTnn'n_nnAGG Cuts at: 2133 2225 2287 2133 Size: 92 62 5754 Fragments arranged by size: 5754 92 62 EcoO109I rG'GnC_Cy Cuts at: 2876 2912 2990 2876 Size: 36 78 5794 Fragments arranged by size: 5794 78 36 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2600 2600 Size: 5908 FspI TGC'GCA Cuts at: 3038 4414 3038 Size: 1376 4532 HaeII r_GCGC'y Cuts at: 1678 2426 2848 2887 3148 5055 1678 Size: 748 422 39 261 1907 2531 Fragments arranged by size: 2531 1907 748 422 261 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 4549 4581 4623 Size: 32 135 32 697 32 2248 32 42 Cuts at: 4623 4655 1373 Size: 32 2626 Fragments arranged by size: 2626 2248 697 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KasI G'GCGC_C Cuts at: 2844 3144 2844 Size: 300 5608 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 5022 5206 1305 Size: 888 2829 184 2007 Fragments arranged by size: 2829 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3866 4225 4384 518 Size: 648 2700 359 159 2042 Fragments arranged by size: 2700 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 2850 2940 3324 4001 5149 5394 1606 Size: 657 587 90 384 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 587 384 245 90 NarI GG'CG_CC Cuts at: 2845 3145 2845 Size: 300 5608 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4399 Fragments arranged by size: 4399 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 2452 3266 4811 1132 Size: 97 1223 814 1545 2229 Fragments arranged by size: 2229 1545 1223 814 97 PasI CC'CwG_GG Cuts at: 1348 2872 1348 Size: 1524 4384 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 2728 2872 3124 1108 Size: 1620 144 252 3892 Fragments arranged by size: 3892 1620 252 144 PfoI T'CCnGG_A Cuts at: 2044 2727 2044 Size: 683 5225 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PmlI CAC'GTG Cuts at: 2526 2526 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2912 2912 Size: 5908 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PspOMI G'GGCC_C Cuts at: 2875 2990 2875 Size: 115 5793 PstI C_TGCA'G Cuts at: 830 2658 3060 3260 830 Size: 1828 402 200 3478 Fragments arranged by size: 3478 1828 402 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2263 2263 Size: 5908 SacI G_AGCT'C Cuts at: 2034 2706 2034 Size: 672 5236 SacII CC_GC'GG Cuts at: 2851 2851 Size: 5908 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SapI GCTCTTCn'nnn_ Cuts at: 2118 2571 2118 Size: 453 5455 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SexAI A'CCwGG_T Cuts at: 2247 2247 Size: 5908 SfcI C'TryA_G Cuts at: 826 1045 2258 2297 2654 3056 3256 4391 5072 Size: 219 1213 39 357 402 200 1135 681 Cuts at: 5072 5263 826 Size: 191 1471 Fragments arranged by size: 1471 1213 1135 681 402 357 219 200 191 39 SfoI GGC'GCC Cuts at: 2846 3146 2846 Size: 300 5608 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 3235 1872 Size: 1363 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2463 2705 4012 4913 5175 820 Size: 197 926 520 242 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 520 262 242 197 SnaBI TAC'GTA Cuts at: 493 3205 3699 493 Size: 2712 494 2702 Fragments arranged by size: 2712 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 2452 3266 1132 Size: 1320 814 3774 Fragments arranged by size: 3774 1320 814 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 3090 1517 Size: 1573 4335 StyI C'CwwG_G Cuts at: 513 1065 2022 2105 2745 3347 513 Size: 552 957 83 640 602 3074 Fragments arranged by size: 3074 957 640 602 552 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3935 3952 4105 4290 4735 325 Size: 1255 2355 17 153 185 445 1498 Fragments arranged by size: 2355 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4154 5866 96 Size: 274 80 33 51 1443 2177 1712 138 Fragments arranged by size: 2177 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2510 2542 5868 5900 2510 Size: 32 3326 32 2518 Fragments arranged by size: 3326 2518 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3101 1316 Size: 1785 4123 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 2463 1943 Size: 520 5388 XmaI C'CCGG_G Cuts at: 1870 3233 1870 Size: 1363 4545 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI