(Circular) (Six-base) MAPSORT of: pFN21ASDA1719.seq Check: 7508 from: 1 to: 7858 pFN21ASDA1719 7858 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3163 3520 4183 3163 Size: 357 663 6838 Fragments arranged by size: 6838 663 357 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2469 5668 278 Size: 53 83 186 936 933 3199 2468 Fragments arranged by size: 3199 2468 936 933 186 83 53 AccI GT'mk_AC Cuts at: 1937 5201 1937 Size: 3264 4594 Acc65I G'GTAC_C Cuts at: 5179 5179 Size: 7858 AclI AA'CG_TT Cuts at: 1563 4146 5985 6358 1563 Size: 2583 1839 373 3063 Fragments arranged by size: 3063 2583 1839 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 3947 4019 4238 5921 7305 7573 1632 Size: 989 1326 72 219 1683 1384 268 1917 Fragments arranged by size: 1917 1683 1384 1326 989 268 219 72 AfeI AGC'GCT Cuts at: 4004 4004 Size: 7858 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7661 AflIII A'CryG_T Cuts at: 1203 6757 7734 1203 Size: 5554 977 1327 Fragments arranged by size: 5554 1327 977 AgeI A'CCGG_T Cuts at: 6689 6689 Size: 7858 AhdI GACnn_n'nnGTC Cuts at: 4663 6587 4663 Size: 1924 5934 AleI CACnn'nnGTG Cuts at: 1166 2165 1166 Size: 999 6859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7858 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3632 3664 3632 Size: 32 7826 AlwNI CAG_nnn'CTG Cuts at: 2792 3604 4604 5601 7173 2792 Size: 812 1000 997 1572 3477 Fragments arranged by size: 3477 1572 1000 997 812 ApaI G_GGCC'C Cuts at: 2073 4418 5116 2073 Size: 2345 698 4815 Fragments arranged by size: 4815 2345 698 ApaLI G'TGCA_C Cuts at: 3371 5915 7071 3371 Size: 2544 1156 4158 Fragments arranged by size: 4158 2544 1156 ApoI r'AATT_y Cuts at: 1493 5167 5393 5545 7654 1493 Size: 3674 226 152 2109 1697 Fragments arranged by size: 3674 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6412 160 Size: 872 5380 1606 Fragments arranged by size: 5380 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7858 AvaI C'yCGr_G Cuts at: 1870 1943 4446 4614 4739 5183 1870 Size: 73 2503 168 125 444 4545 Fragments arranged by size: 4545 2503 444 168 125 73 BamHI G'GATC_C Cuts at: 2325 3257 5188 5555 2325 Size: 932 1931 367 4628 Fragments arranged by size: 4628 1931 932 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2219 2516 2927 3683 3815 Size: 325 217 627 432 297 411 756 132 Cuts at: 3815 3974 4106 4718 5035 5179 6634 618 Size: 159 132 612 317 144 1455 1842 Fragments arranged by size: 1842 1455 756 627 612 432 411 325 317 297 217 159 144 132 132 BanII G_rGCy'C Cuts at: 1391 2037 2073 2597 2893 3326 3768 4418 4512 Size: 646 36 524 296 433 442 650 94 Cuts at: 4512 4598 5116 5177 1391 Size: 86 518 61 4072 Fragments arranged by size: 4072 650 646 524 518 442 433 296 94 86 61 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3177 4662 928 Size: 971 1278 1485 4124 Fragments arranged by size: 4124 1485 1278 971 BbvCI CC'TCA_GC Cuts at: 3413 4484 4688 3413 Size: 1071 204 6583 Fragments arranged by size: 6583 1071 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6049 6083 1903 Size: 34 4112 34 3678 Fragments arranged by size: 4112 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5750 6960 5750 Size: 1210 6648 BclI T'GATC_A Cuts at: 1548 2175 3771 5073 1548 Size: 627 1596 1302 4333 Fragments arranged by size: 4333 1596 1302 627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3037 3361 3620 Size: 107 122 71 631 605 1365 324 259 Cuts at: 3620 4158 4480 6469 136 Size: 538 322 1989 1525 Fragments arranged by size: 1989 1525 1365 631 605 538 324 322 259 122 107 71 BglII A'GATC_T Cuts at: 7853 7853 Size: 7858 BlpI GC'TnA_GC Cuts at: 2036 2621 4169 5275 2036 Size: 585 1548 1106 4619 Fragments arranged by size: 4619 1548 1106 585 Bme1580I G_kGCm'C Cuts at: 1790 2073 2521 2535 3018 3102 3375 3818 4040 Size: 283 448 14 483 84 273 443 222 Cuts at: 4040 4356 4418 4454 4721 5116 5919 7075 1790 Size: 316 62 36 267 395 803 1156 2573 Fragments arranged by size: 2573 1156 803 483 448 443 395 316 283 273 267 222 84 62 36 14 BmgBI CAC'GTC Cuts at: 1318 5002 1318 Size: 3684 4174 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4350 5589 6547 Size: 416 53 83 399 24 2933 1239 958 Cuts at: 6547 442 Size: 1753 Fragments arranged by size: 2933 1753 1239 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3338 1056 Size: 2282 5576 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3967 4667 3428 Size: 539 700 6619 Fragments arranged by size: 6619 700 539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 2417 2922 3020 3325 3413 3419 3659 Size: 834 344 505 98 305 88 6 240 Cuts at: 3659 3842 3983 4221 4682 4718 4952 6518 1239 Size: 183 141 238 461 36 234 1566 2579 Fragments arranged by size: 2579 1566 834 505 461 344 305 240 238 234 183 141 98 88 36 6 Bpu10I CC'TnA_GC Cuts at: 3413 4484 4688 3413 Size: 1071 204 6583 Fragments arranged by size: 6583 1071 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2354 2975 4139 5983 6848 7146 2354 Size: 621 1164 1844 865 298 3066 Fragments arranged by size: 3066 1844 1164 865 621 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2039 2251 2898 3374 3399 3452 3959 Size: 617 540 212 647 476 25 53 507 Cuts at: 3959 4199 4271 6521 882 Size: 240 72 2250 2219 Fragments arranged by size: 2250 2219 647 617 540 507 476 240 212 72 53 25 BsaAI yAC'GTr Cuts at: 493 1204 2563 3694 4117 5155 5649 493 Size: 711 1359 1131 423 1038 494 2702 Fragments arranged by size: 2702 1359 1131 1038 711 494 423 BsaBI GATnn'nnATC Cuts at: 7852 7852 Size: 7858 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2466 2783 3612 3885 Size: 53 83 186 936 933 317 829 273 Cuts at: 3885 5665 6047 6682 275 Size: 1780 382 635 1451 Fragments arranged by size: 1780 1451 936 933 829 635 382 317 273 186 83 53 BsaWI w'CCGG_w Cuts at: 2826 3933 6291 6689 6963 7110 7521 2826 Size: 1107 2358 398 274 147 411 3163 Fragments arranged by size: 3163 2358 1107 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 3632 3662 4194 Size: 30 165 30 987 30 1212 30 532 Cuts at: 4194 4224 4799 4829 1178 Size: 30 575 30 4207 Fragments arranged by size: 4207 1212 987 575 532 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2526 3079 3310 4574 4577 4580 4634 Size: 973 367 553 231 1264 3 3 54 Cuts at: 4634 4823 1186 Size: 189 4221 Fragments arranged by size: 4221 1264 973 553 367 231 189 54 3 3 BseYI C'CCAG_C Cuts at: 2197 3084 3239 3261 3724 4300 4537 4597 5132 Size: 887 155 22 463 576 237 60 535 Cuts at: 5132 7061 7557 2197 Size: 1929 496 2498 Fragments arranged by size: 2498 1929 887 576 535 496 463 237 155 60 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 2707 2879 3062 3080 4745 4838 Size: 573 891 80 172 183 18 1665 93 Cuts at: 4838 1163 Size: 4183 Fragments arranged by size: 4183 1665 891 573 183 172 93 80 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2854 4166 5123 5308 6069 7097 Size: 275 186 853 1312 957 185 761 1028 Cuts at: 7097 1540 Size: 2301 Fragments arranged by size: 2301 1312 1028 957 853 761 275 186 185 BsiHKAI G_wGCw'C Cuts at: 3326 3375 3399 5919 6004 7075 3326 Size: 49 24 2520 85 1071 4109 Fragments arranged by size: 4109 2520 1071 85 49 24 BsmI GAATG_Cn' Cuts at: 2136 4128 5369 5462 2136 Size: 1992 1241 93 4532 Fragments arranged by size: 4532 1992 1241 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2473 4897 2473 Size: 2424 5434 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2073 2521 2535 2597 2893 3018 Size: 399 247 36 448 14 62 296 125 Cuts at: 3018 3102 3326 3375 3399 3768 3818 4040 4356 Size: 84 224 49 24 369 50 222 316 Cuts at: 4356 4418 4454 4512 4598 4721 5116 5177 5919 Size: 62 36 58 86 123 395 61 742 Cuts at: 5919 6004 7075 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 395 369 316 296 247 224 222 125 123 86 85 84 62 62 61 58 50 49 36 36 24 14 BspEI T'CCGG_A Cuts at: 2826 3933 7521 2826 Size: 1107 3588 3163 Fragments arranged by size: 3588 3163 1107 BspHI T'CATG_A Cuts at: 4710 5747 7477 4710 Size: 1037 1730 5091 Fragments arranged by size: 5091 1730 1037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3163 3520 4183 4981 5213 844 Size: 2319 357 663 798 232 3489 Fragments arranged by size: 3489 2319 798 663 357 232 BsrBI CCG'CTC Cuts at: 1394 1823 2426 2886 4468 5745 1394 Size: 429 603 460 1582 1277 3507 Fragments arranged by size: 3507 1582 1277 603 460 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3561 6353 6527 65 Size: 1183 2313 2792 174 1396 Fragments arranged by size: 2792 2313 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4161 4345 4758 6502 6689 1700 Size: 110 2351 184 413 1744 187 2869 Fragments arranged by size: 2869 2351 1744 413 187 184 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7858 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7858 BssSI C'ACGA_G Cuts at: 2693 3479 5918 6930 2693 Size: 786 2439 1012 3621 Fragments arranged by size: 3621 2439 1012 786 BstAPI GCAn_nnn'nTGC Cuts at: 794 3370 3898 794 Size: 2576 528 4754 Fragments arranged by size: 4754 2576 528 BstBI TT'CG_AA Cuts at: 2007 6662 2007 Size: 4655 3203 BstEII G'GTnAC_C Cuts at: 1184 4846 1184 Size: 3662 4196 BstXI CCAn_nnnn'nTGG Cuts at: 2528 2668 2875 2971 3349 4117 2528 Size: 140 207 96 378 768 6269 Fragments arranged by size: 6269 768 378 207 140 96 BstYI r'GATC_y Cuts at: 1972 2325 2802 3003 3257 3930 5188 5555 5940 Size: 353 477 201 254 673 1258 367 385 Cuts at: 5940 5957 7409 7495 7507 7853 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1258 673 477 385 367 353 346 254 201 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3253 3384 3876 4957 513 Size: 552 2188 131 492 1081 3414 Fragments arranged by size: 3414 2188 1081 552 492 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7119 Fragments arranged by size: 7119 683 56 BtsI GCAGTG_nn' Cuts at: 2690 2941 3057 3127 3685 3911 5374 6168 6188 Size: 251 116 70 558 226 1463 794 20 Cuts at: 6188 2690 Size: 4360 Fragments arranged by size: 4360 1463 794 558 251 226 116 70 20 ClaI AT'CG_AT Cuts at: 1554 2922 1554 Size: 1368 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2442 2477 4745 4780 529 Size: 35 1878 35 2268 35 3607 Fragments arranged by size: 3607 2268 1878 35 35 35 DraI TTT'AAA Cuts at: 5162 5162 Size: 7858 DraIII CAC_nnn'GTG Cuts at: 4846 4933 4846 Size: 87 7771 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2788 4061 6865 809 Size: 507 1472 1273 2804 1802 Fragments arranged by size: 2804 1802 1472 1273 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2670 2724 2973 Size: 54 1425 50 275 18 840 54 249 Cuts at: 2973 3029 3124 3160 3744 3843 4134 4163 4392 Size: 56 95 36 584 99 291 29 229 Cuts at: 4392 4753 5046 5305 6194 7823 8 Size: 361 293 259 889 1629 43 Fragments arranged by size: 1629 1425 889 840 584 361 293 291 275 259 249 229 99 95 56 54 54 50 43 36 29 18 EagI C'GGCC_G Cuts at: 1537 1812 4163 5305 1537 Size: 275 2351 1142 4090 Fragments arranged by size: 4090 2351 1142 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5788 1353 Size: 4435 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 2333 3307 3674 3848 6445 Size: 1064 138 486 258 974 367 174 2597 Cuts at: 6445 6819 6965 387 Size: 374 146 1280 Fragments arranged by size: 2597 1280 1064 974 486 374 367 258 174 146 138 EcoICRI GAG'CTC Cuts at: 3324 3324 Size: 7858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2417 2621 2922 3020 3325 3413 Size: 393 441 344 204 301 98 305 88 Cuts at: 3413 3419 3659 3842 3947 3983 4019 4221 4238 Size: 6 240 183 105 36 36 202 17 Cuts at: 4238 4682 4718 4952 5921 6518 7305 7573 1239 Size: 444 36 234 969 597 787 268 1524 Fragments arranged by size: 1524 969 787 597 444 441 393 344 305 301 268 240 234 204 202 183 105 98 88 36 36 36 17 6 EcoNI CCTnn'n_nnAGG Cuts at: 2477 4190 4856 4978 5048 2477 Size: 1713 666 122 70 5287 Fragments arranged by size: 5287 1713 666 122 70 EcoO109I rG'GnC_Cy Cuts at: 2070 2756 3039 3088 3913 4013 4297 4414 4422 Size: 686 283 49 825 100 284 117 8 Cuts at: 4422 4861 4984 5113 5129 2070 Size: 439 123 129 16 4799 Fragments arranged by size: 4799 825 686 439 284 283 129 123 117 100 49 16 8 EcoRI G'AATT_C Cuts at: 5167 5545 5167 Size: 378 7480 FseI GG_CCGG'CC Cuts at: 4165 4165 Size: 7858 FspI TGC'GCA Cuts at: 6364 6364 Size: 7858 HaeII r_GCGC'y Cuts at: 1678 4006 7005 1678 Size: 2328 2999 2531 Fragments arranged by size: 2999 2531 2328 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2895 2927 3449 3481 4194 Size: 32 135 32 1323 32 522 32 713 Cuts at: 4194 4226 4649 4681 6499 6531 6573 6605 1373 Size: 32 423 32 1818 32 42 32 2626 Fragments arranged by size: 2626 1818 1323 713 522 423 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4996 5202 5448 669 Size: 988 281 3058 206 246 3079 Fragments arranged by size: 3079 3058 988 281 246 206 HindIII A'AGCT_T Cuts at: 748 748 Size: 7858 HpaI GTT'AAC Cuts at: 5448 5448 Size: 7858 KpnI G_GTAC'C Cuts at: 5183 5183 Size: 7858 MfeI C'AATT_G Cuts at: 5457 5457 Size: 7858 MluI A'CGCG_T Cuts at: 7734 7734 Size: 7858 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 3804 6972 7156 1305 Size: 969 1530 3168 184 2007 Fragments arranged by size: 3168 2007 1530 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2672 2726 2975 3162 3845 Size: 54 1425 343 840 54 249 187 683 Cuts at: 3845 4136 4755 10 Size: 291 619 3113 Fragments arranged by size: 3113 1425 840 683 619 343 291 249 187 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2526 2933 4115 5816 6175 6334 Size: 648 999 361 407 1182 1701 359 159 Cuts at: 6334 518 Size: 2042 Fragments arranged by size: 2042 1701 1182 999 648 407 361 359 159 MspA1I CmG'CkG Cuts at: 1606 2641 3028 3076 3084 3417 4168 4541 5274 Size: 1035 387 48 8 333 751 373 733 Cuts at: 5274 5951 7099 7344 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1035 751 733 677 387 373 333 245 48 8 NaeI GCC'GGC Cuts at: 4163 4347 4163 Size: 184 7674 NcoI C'CATG_G Cuts at: 513 1065 3876 513 Size: 552 2811 4495 Fragments arranged by size: 4495 2811 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7858 NgoMIV G'CCGG_C Cuts at: 4161 4345 4161 Size: 184 7674 NheI G'CTAG_C Cuts at: 1052 3334 1052 Size: 2282 5576 NotI GC'GGCC_GC Cuts at: 5305 5305 Size: 7858 NspI r_CATG'y Cuts at: 1132 1229 4639 5216 6761 1132 Size: 97 3410 577 1545 2229 Fragments arranged by size: 3410 2229 1545 577 97 PasI CC'CwG_GG Cuts at: 1348 2241 2797 4018 4418 1348 Size: 893 556 1221 400 4788 Fragments arranged by size: 4788 1221 893 556 400 PciI A'CATG_T Cuts at: 6757 6757 Size: 7858 PflMI CCAn_nnn'nTGG Cuts at: 1108 3680 4604 1108 Size: 2572 924 4362 Fragments arranged by size: 4362 2572 924 PfoI T'CCnGG_A Cuts at: 4905 4905 Size: 7858 PmeI GTTT'AAAC Cuts at: 5162 5162 Size: 7858 PmlI CAC'GTG Cuts at: 3694 4117 3694 Size: 423 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3632 3664 5927 5959 7440 7472 3632 Size: 32 2263 32 1481 32 4018 Fragments arranged by size: 4018 2263 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2756 3088 3913 4013 4297 4422 4861 4984 2756 Size: 332 825 100 284 125 439 123 5630 Fragments arranged by size: 5630 825 439 332 284 125 123 100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7858 PsiI TTA'TAA Cuts at: 5428 5428 Size: 7858 PspOMI G'GGCC_C Cuts at: 2069 4414 5112 2069 Size: 2345 698 4815 Fragments arranged by size: 4815 2345 698 PspXI vC'TCGA_Gb Cuts at: 4739 4739 Size: 7858 PstI C_TGCA'G Cuts at: 830 3134 3531 4194 4801 4992 5210 830 Size: 2304 397 663 607 191 218 3478 Fragments arranged by size: 3478 2304 663 607 397 218 191 PvuI CG_AT'CG Cuts at: 2001 5123 2001 Size: 3122 4736 PvuII CAG'CTG Cuts at: 3076 4541 3076 Size: 1465 6393 SacI G_AGCT'C Cuts at: 3326 3326 Size: 7858 SalI G'TCGA_C Cuts at: 1936 5200 1936 Size: 3264 4594 SanDI GG'GwC_CC Cuts at: 2756 3088 4013 2756 Size: 332 925 6601 Fragments arranged by size: 6601 925 332 SbfI CC_TGCA'GG Cuts at: 4194 4992 5210 4194 Size: 798 218 6842 Fragments arranged by size: 6842 798 218 ScaI AGT'ACT Cuts at: 6106 7818 6106 Size: 1712 6146 SexAI A'CCwGG_T Cuts at: 3915 3915 Size: 7858 SfcI C'TryA_G Cuts at: 826 1045 3130 3527 4190 4797 4988 5206 6341 Size: 219 2085 397 663 607 191 218 1135 Cuts at: 6341 7022 7213 826 Size: 681 191 1471 Fragments arranged by size: 2085 1471 1135 681 663 607 397 219 218 191 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3037 3037 Size: 7858 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7858 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7858 SmaI CCC'GGG Cuts at: 1872 4448 5185 1872 Size: 2576 737 4545 Fragments arranged by size: 4545 2576 737 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 2954 4154 4739 5962 6863 Size: 197 926 426 585 1200 585 1223 901 Cuts at: 6863 7125 820 Size: 262 1553 Fragments arranged by size: 1553 1223 1200 926 901 585 585 426 262 197 SnaBI TAC'GTA Cuts at: 493 5155 5649 493 Size: 4662 494 2702 Fragments arranged by size: 4662 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7858 SphI G_CATG'C Cuts at: 1132 5216 1132 Size: 4084 3774 SspI AAT'ATT Cuts at: 5 52 5782 5 Size: 47 5730 2081 Fragments arranged by size: 5730 2081 47 StuI AGG'CCT Cuts at: 1517 4074 4323 4474 4750 4965 1517 Size: 2557 249 151 276 215 4410 Fragments arranged by size: 4410 2557 276 249 215 151 StyI C'CwwG_G Cuts at: 513 1065 3876 4763 4830 5297 513 Size: 552 2811 887 67 467 3074 Fragments arranged by size: 3074 2811 887 552 467 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2301 4516 5885 5902 6055 6240 6685 Size: 1255 721 2215 1369 17 153 185 445 Cuts at: 6685 325 Size: 1498 Fragments arranged by size: 2215 1498 1369 1255 721 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6104 7816 96 Size: 274 80 33 51 1443 4127 1712 138 Fragments arranged by size: 4127 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2900 2932 3209 3241 7818 7850 2900 Size: 32 277 32 4577 32 2908 Fragments arranged by size: 4577 2908 277 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3463 3542 4982 1316 Size: 2147 79 1440 4192 Fragments arranged by size: 4192 2147 1440 79 XbaI T'CTAG_A Cuts at: 5194 5194 Size: 7858 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2968 4087 2968 Size: 1119 6739 XhoI C'TCGA_G Cuts at: 1943 4739 1943 Size: 2796 5062 XmaI C'CCGG_G Cuts at: 1870 4446 5183 1870 Size: 2576 737 4545 Fragments arranged by size: 4545 2576 737 XmnI GAAnn'nnTTC Cuts at: 5987 5987 Size: 7858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2467 5666 276 Size: 53 83 186 936 933 3199 2468 Fragments arranged by size: 3199 2468 936 933 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SapI SfoI SrfI SwaI