Restriction map for
pFN21ASDA1719
(Linear) (Six-base) MAPSORT of: KIAA1719.seq Check: 7625 from: 1 to: 3129
KIAA1719.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1140 1497 2160 3129
Size: 1140 357 663 969
Fragments arranged by size:
1140 969 663 357
AatII G_ACGT'C
Cuts at: 0 446 3129
Size: 446 2683
AclI AA'CG_TT
Cuts at: 0 2123 3129
Size: 2123 1006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1924 1996 2215 3129
Size: 598 1326 72 219 914
Fragments arranged by size:
1326 914 598 219 72
AfeI AGC'GCT
Cuts at: 0 1981 3129
Size: 1981 1148
AhdI GACnn_n'nnGTC
Cuts at: 0 2640 3129
Size: 2640 489
AleI CACnn'nnGTG
Cuts at: 0 142 3129
Size: 142 2987
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 3129
Size: 1609 32 1488
Fragments arranged by size:
1609 1488 32
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1581 2581 3129
Size: 769 812 1000 548
Fragments arranged by size:
1000 812 769 548
ApaI G_GGCC'C
Cuts at: 0 50 2395 3093 3129
Size: 50 2345 698 36
Fragments arranged by size:
2345 698 50 36
ApaLI G'TGCA_C
Cuts at: 0 1348 3129
Size: 1348 1781
AvaI C'yCGr_G
Cuts at: 0 2423 2591 2716 3129
Size: 2423 168 125 413
Fragments arranged by size:
2423 413 168 125
BamHI G'GATC_C
Cuts at: 0 302 1234 3129
Size: 302 932 1895
Fragments arranged by size:
1895 932 302
BanI G'GyrC_C
Cuts at: 0 196 493 904 1660 1792 1951 2083 2695
Size: 196 297 411 756 132 159 132 612
Cuts at: 2695 3012 3129
Size: 317 117
Fragments arranged by size:
756 612 411 317 297 196 159 132
132 117
BanII G_rGCy'C
Cuts at: 0 14 50 574 870 1303 1745 2395 2489
Size: 14 36 524 296 433 442 650 94
Cuts at: 2489 2575 3093 3129
Size: 86 518 36
Fragments arranged by size:
650 524 518 442 433 296 94 86
36 36 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 1154 2639 3129
Size: 1154 1485 490
Fragments arranged by size:
1485 1154 490
BbvCI CC'TCA_GC
Cuts at: 0 1390 2461 2665 3129
Size: 1390 1071 204 464
Fragments arranged by size:
1390 1071 464 204
BclI T'GATC_A
Cuts at: 0 152 1748 3050 3129
Size: 152 1596 1302 79
Fragments arranged by size:
1596 1302 152 79
BglI GCCn_nnn'nGGC
Cuts at: 0 1014 1338 1597 2135 2457 3129
Size: 1014 324 259 538 322 672
Fragments arranged by size:
1014 672 538 324 322 259
BlpI GC'TnA_GC
Cuts at: 0 13 598 2146 3129
Size: 13 585 1548 983
Fragments arranged by size:
1548 983 585 13
Bme1580I G_kGCm'C
Cuts at: 0 50 498 512 995 1079 1352 1795 2017
Size: 50 448 14 483 84 273 443 222
Cuts at: 2017 2333 2395 2431 2698 3093 3129
Size: 316 62 36 267 395 36
Fragments arranged by size:
483 448 443 395 316 273 267 222
84 62 50 36 36 14
BmgBI CAC'GTC
Cuts at: 0 2979 3129
Size: 2979 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 2327 3129
Size: 2327 802
BmtI G_CTAG'C
Cuts at: 0 1315 3129
Size: 1315 1814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1405 1944 2644 3129
Size: 1405 539 700 485
Fragments arranged by size:
1405 700 539 485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 394 899 997 1302 1390 1396 1636
Size: 50 344 505 98 305 88 6 240
Cuts at: 1636 1819 1960 2198 2659 2695 2929 3129
Size: 183 141 238 461 36 234 200
Fragments arranged by size:
505 461 344 305 240 238 234 200
183 141 98 88 50 36 6
Bpu10I CC'TnA_GC
Cuts at: 0 1390 2461 2665 3129
Size: 1390 1071 204 464
Fragments arranged by size:
1390 1071 464 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 952 2116 3129
Size: 331 621 1164 1013
Fragments arranged by size:
1164 1013 621 331
BsaI GGTCTCn'nnnn_
Cuts at: 0 16 228 875 1351 1376 1429 1936 2176
Size: 16 212 647 476 25 53 507 240
Cuts at: 2176 2248 3129
Size: 72 881
Fragments arranged by size:
881 647 507 476 240 212 72 53
25 16
BsaAI yAC'GTr
Cuts at: 0 540 1671 2094 3129
Size: 540 1131 423 1035
Fragments arranged by size:
1131 1035 540 423
BsaHI Gr'CG_yC
Cuts at: 0 443 760 1589 1862 3129
Size: 443 317 829 273 1267
Fragments arranged by size:
1267 829 443 317 273
BsaWI w'CCGG_w
Cuts at: 0 803 1910 3129
Size: 803 1107 1219
Fragments arranged by size:
1219 1107 803
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 1609 1639 2171 2201 2776 2806
Size: 367 30 1212 30 532 30 575 30
Cuts at: 2806 3129
Size: 323
Fragments arranged by size:
1212 575 532 367 323 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 503 1056 1287 2551 2554 2557 2611
Size: 136 367 553 231 1264 3 3 54
Cuts at: 2611 2800 3129
Size: 189 329
Fragments arranged by size:
1264 553 367 329 231 189 136 54
3 3
BseYI C'CCAG_C
Cuts at: 0 174 1061 1216 1238 1701 2277 2514 2574
Size: 174 887 155 22 463 576 237 60
Cuts at: 2574 3109 3129
Size: 535 20
Fragments arranged by size:
887 576 535 463 237 174 155 60
22 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 684 856 1039 1057 2722 2815 3129
Size: 604 80 172 183 18 1665 93 314
Fragments arranged by size:
1665 604 314 183 172 93 80 18
BsiEI CG_ry'CG
Cuts at: 0 831 2143 3100 3129
Size: 831 1312 957 29
Fragments arranged by size:
1312 957 831 29
BsiHKAI G_wGCw'C
Cuts at: 0 1303 1352 1376 3129
Size: 1303 49 24 1753
Fragments arranged by size:
1753 1303 49 24
BsmI GAATG_Cn'
Cuts at: 0 113 2105 3129
Size: 113 1992 1024
Fragments arranged by size:
1992 1024 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 450 2874 3129
Size: 450 2424 255
Fragments arranged by size:
2424 450 255
Bsp1286I G_dGCh'C
Cuts at: 0 14 50 498 512 574 870 995 1079
Size: 14 36 448 14 62 296 125 84
Cuts at: 1079 1303 1352 1376 1745 1795 2017 2333 2395
Size: 224 49 24 369 50 222 316 62
Cuts at: 2395 2431 2489 2575 2698 3093 3129
Size: 36 58 86 123 395 36
Fragments arranged by size:
448 395 369 316 296 224 222 125
123 86 84 62 62 58 50 49
36 36 36 24 14 14
BspEI T'CCGG_A
Cuts at: 0 803 1910 3129
Size: 803 1107 1219
Fragments arranged by size:
1219 1107 803
BspHI T'CATG_A
Cuts at: 0 2687 3129
Size: 2687 442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1140 1497 2160 2958 3129
Size: 1140 357 663 798 171
Fragments arranged by size:
1140 798 663 357 171
BsrBI CCG'CTC
Cuts at: 0 403 863 2445 3129
Size: 403 460 1582 684
Fragments arranged by size:
1582 684 460 403
BsrDI GCAATG_nn'
Cuts at: 0 1538 3129
Size: 1538 1591
BsrFI r'CCGG_y
Cuts at: 0 2138 2322 2735 3129
Size: 2138 184 413 394
Fragments arranged by size:
2138 413 394 184
BssSI C'ACGA_G
Cuts at: 0 670 1456 3129
Size: 670 786 1673
Fragments arranged by size:
1673 786 670
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1347 1875 3129
Size: 1347 528 1254
Fragments arranged by size:
1347 1254 528
BstEII G'GTnAC_C
Cuts at: 0 2823 3129
Size: 2823 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 505 645 852 948 1326 2094 3129
Size: 505 140 207 96 378 768 1035
Fragments arranged by size:
1035 768 505 378 207 140 96
BstYI r'GATC_y
Cuts at: 0 302 779 980 1234 1907 3129
Size: 302 477 201 254 673 1222
Fragments arranged by size:
1222 673 477 302 254 201
BtgI C'CryG_G
Cuts at: 0 1230 1361 1853 2934 3129
Size: 1230 131 492 1081 195
Fragments arranged by size:
1230 1081 492 195 131
BtsI GCAGTG_nn'
Cuts at: 0 667 918 1034 1104 1662 1888 3129
Size: 667 251 116 70 558 226 1241
Fragments arranged by size:
1241 667 558 251 226 116 70
ClaI AT'CG_AT
Cuts at: 0 899 3129
Size: 899 2230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 419 454 2722 2757 3129
Size: 419 35 2268 35 372
Fragments arranged by size:
2268 419 372 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2823 2910 3129
Size: 2823 87 219
Fragments arranged by size:
2823 219 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 765 2038 3129
Size: 765 1273 1091
Fragments arranged by size:
1273 1091 765
EaeI y'GGCC_r
Cuts at: 0 647 701 950 1006 1101 1137 1721 1820
Size: 647 54 249 56 95 36 584 99
Cuts at: 1820 2111 2140 2369 2730 3023 3129
Size: 291 29 229 361 293 106
Fragments arranged by size:
647 584 361 293 291 249 229 106
99 95 56 54 36 29
EagI C'GGCC_G
Cuts at: 0 2140 3129
Size: 2140 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 310 1284 1651 1825 3129
Size: 52 258 974 367 174 1304
Fragments arranged by size:
1304 974 367 258 174 52
EcoICRI GAG'CTC
Cuts at: 0 1301 3129
Size: 1301 1828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 394 598 899 997 1302 1390 1396
Size: 50 344 204 301 98 305 88 6
Cuts at: 1396 1636 1819 1924 1960 1996 2198 2215 2659
Size: 240 183 105 36 36 202 17 444
Cuts at: 2659 2695 2929 3129
Size: 36 234 200
Fragments arranged by size:
444 344 305 301 240 234 204 202
200 183 105 98 88 50 36 36
36 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 2167 2833 2955 3025 3129
Size: 454 1713 666 122 70 104
Fragments arranged by size:
1713 666 454 122 104 70
EcoO109I rG'GnC_Cy
Cuts at: 0 47 733 1016 1065 1890 1990 2274 2391
Size: 47 686 283 49 825 100 284 117
Cuts at: 2391 2399 2838 2961 3090 3106 3129
Size: 8 439 123 129 16 23
Fragments arranged by size:
825 686 439 284 283 129 123 117
100 49 47 23 16 8
FseI GG_CCGG'CC
Cuts at: 0 2142 3129
Size: 2142 987
HaeII r_GCGC'y
Cuts at: 0 1983 3129
Size: 1983 1146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 872 904 1426 1458 2171 2203 2626 2658
Size: 872 32 522 32 713 32 423 32
Cuts at: 2658 3129
Size: 471
Fragments arranged by size:
872 713 522 471 423 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2973 3129
Size: 2973 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1781 3129
Size: 251 1530 1348
Fragments arranged by size:
1530 1348 251
MscI TGG'CCA
Cuts at: 0 649 703 952 1139 1822 2113 2732 3129
Size: 649 54 249 187 683 291 619 397
Fragments arranged by size:
683 649 619 397 291 249 187 54
MslI CAynn'nnrTG
Cuts at: 0 142 503 910 2092 3129
Size: 142 361 407 1182 1037
Fragments arranged by size:
1182 1037 407 361 142
MspA1I CmG'CkG
Cuts at: 0 618 1005 1053 1061 1394 2145 2518 3129
Size: 618 387 48 8 333 751 373 611
Fragments arranged by size:
751 618 611 387 373 333 48 8
NaeI GCC'GGC
Cuts at: 0 2140 2324 3129
Size: 2140 184 805
Fragments arranged by size:
2140 805 184
NcoI C'CATG_G
Cuts at: 0 1853 3129
Size: 1853 1276
NgoMIV G'CCGG_C
Cuts at: 0 2138 2322 3129
Size: 2138 184 807
Fragments arranged by size:
2138 807 184
NheI G'CTAG_C
Cuts at: 0 1311 3129
Size: 1311 1818
NspI r_CATG'y
Cuts at: 0 2616 3129
Size: 2616 513
PasI CC'CwG_GG
Cuts at: 0 218 774 1995 2395 3129
Size: 218 556 1221 400 734
Fragments arranged by size:
1221 734 556 400 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 1657 2581 3129
Size: 1657 924 548
Fragments arranged by size:
1657 924 548
PfoI T'CCnGG_A
Cuts at: 0 2882 3129
Size: 2882 247
PmlI CAC'GTG
Cuts at: 0 1671 2094 3129
Size: 1671 423 1035
Fragments arranged by size:
1671 1035 423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 3129
Size: 1609 32 1488
Fragments arranged by size:
1609 1488 32
PpuMI rG'GwC_Cy
Cuts at: 0 733 1065 1890 1990 2274 2399 2838 2961
Size: 733 332 825 100 284 125 439 123
Cuts at: 2961 3129
Size: 168
Fragments arranged by size:
825 733 439 332 284 168 125 123
100
PspOMI G'GGCC_C
Cuts at: 0 46 2391 3089 3129
Size: 46 2345 698 40
Fragments arranged by size:
2345 698 46 40
PspXI vC'TCGA_Gb
Cuts at: 0 2716 3129
Size: 2716 413
PstI C_TGCA'G
Cuts at: 0 1111 1508 2171 2778 2969 3129
Size: 1111 397 663 607 191 160
Fragments arranged by size:
1111 663 607 397 191 160
PvuI CG_AT'CG
Cuts at: 0 3100 3129
Size: 3100 29
PvuII CAG'CTG
Cuts at: 0 1053 2518 3129
Size: 1053 1465 611
Fragments arranged by size:
1465 1053 611
SacI G_AGCT'C
Cuts at: 0 1303 3129
Size: 1303 1826
SanDI GG'GwC_CC
Cuts at: 0 733 1065 1990 3129
Size: 733 332 925 1139
Fragments arranged by size:
1139 925 733 332
SbfI CC_TGCA'GG
Cuts at: 0 2171 2969 3129
Size: 2171 798 160
Fragments arranged by size:
2171 798 160
SexAI A'CCwGG_T
Cuts at: 0 1892 3129
Size: 1892 1237
SfcI C'TryA_G
Cuts at: 0 1107 1504 2167 2774 2965 3129
Size: 1107 397 663 607 191 164
Fragments arranged by size:
1107 663 607 397 191 164
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1014 3129
Size: 1014 2115
SmaI CCC'GGG
Cuts at: 0 2425 3129
Size: 2425 704
SmlI C'TyrA_G
Cuts at: 0 346 931 2131 2716 3129
Size: 346 585 1200 585 413
Fragments arranged by size:
1200 585 585 413 346
StuI AGG'CCT
Cuts at: 0 2051 2300 2451 2727 2942 3129
Size: 2051 249 151 276 215 187
Fragments arranged by size:
2051 276 249 215 187 151
StyI C'CwwG_G
Cuts at: 0 1853 2740 2807 3129
Size: 1853 887 67 322
Fragments arranged by size:
1853 887 322 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 2493 3129
Size: 278 2215 636
Fragments arranged by size:
2215 636 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 877 909 1186 1218 3129
Size: 877 32 277 32 1911
Fragments arranged by size:
1911 877 277 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1440 1519 2959 3129
Size: 1440 79 1440 170
Fragments arranged by size:
1440 1440 170 79
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 945 2064 3129
Size: 945 1119 1065
Fragments arranged by size:
1119 1065 945
XhoI C'TCGA_G
Cuts at: 0 2716 3129
Size: 2716 413
XmaI C'CCGG_G
Cuts at: 0 2423 3129
Size: 2423 706
ZraI GAC'GTC
Cuts at: 0 444 3129
Size: 444 2685
Enzymes that do cut:
AarI AatII AclI AcuI AfeI AhdI AleI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI
BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII SacI SanDI SbfI SexAI SfcI SfiI SmaI
SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AflII AflIII AgeI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI
BglII BsaBI BsiWI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI DraI EarI EcoRI EcoRV FalI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NotI NruI NsiI PacI PciI PmeI PshAI PsiI
PsrI RsrII SacII SalI SapI ScaI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XmnI