Restriction map for
pFN21ASDA1709
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BfrI
BlnI BsaHI BsgI BsiEI BsiWI
BsmBI BspDI BspHI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FauI FseI
FspI Hin1I HpaI KasI KpnI
KspI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1370 CGCG CG^CG
AflIII 125 ACATGT A^C[GA][CT]GT
AocI 825 CCTGAGG CC^T[ACGT]AGG
Asp700I 2304 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1821 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 401 TGGCCA TGG^CCA
BanII 2779 GAGCCC G[GA]GC[CT]^C
BbvCI 702 GCTGAGG CCTCAGC(-5/-2)
BclI 1064 TGATCA T^GATCA
BglII 67 AGATCT A^GATCT
BlpI 55 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC
BsaAI 1597 TACGTG [CT]AC^GT[GA]
BsaBI 1358 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 325 GAGACC GGTCTC(1/5)
Bsp1407I 479 TGTACA T^GTACA
BspLU11I 125 ACATGT A^CATGT
BsrBI 58 GAGCGG CCGCTC(-3/-3)
BsrDI 1884 CATTGC GCAATG(2/0)
BsrFI 1259 GCCGGT [GA]^CCGG[CT]
BsrGI 479 TGTACA T^GTACA
BssSI 2232 CTCGTG CACGAG(-5/-1)
BstAPI 1935 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1370 CGCG CG^CG
Bsu36I 825 CCTGAGG CC^T[ACGT]AGG
CelII 55 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1259 GCCGGT [GA]^CCGG[CT]
DraI 979 TTTAAA TTT^AAA
Eco81I 825 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1 ATGCAT ATGCA^T
FbaI 1064 TGATCA T^GATCA
HaeII 1303 AGCGCC [GA]GCGC^[CT]
HgaI 2250 GACGC GACGC(5/10)
HincII 1295 GTCAAC GT[CT]^[GA]AC
HindII 1295 GTCAAC GT[CT]^[GA]AC
HindIII 1508 AAGCTT A^AGCTT
MamI 1358 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 475 CAATTG C^AATTG
MluNI 401 TGGCCA TGG^CCA
MscI 401 TGGCCA TGG^CCA
MunI 475 CAATTG C^AATTG
MvnI 1370 CGCG CG^CG
NsiI 1 ATGCAT ATGCA^T
PflFI 1821 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 832 AGTACT AGT^ACT
SpeI 1149 ACTAGT A^CTAGT
SspBI 479 TGTACA T^GTACA
Tth111I 1821 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 771 TCTAGA T^CTAGA
XmnI 2304 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 29 TCCGGA T^CCGGA
669 TCCGGA
AlwNI 1121 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1936 CAGCTGCTG
AspHI 1928 GTGCTC G[AT]GC[AT]^C
2227 GAGCAC
BbsI 2247 GAAGAC GAAGAC(2/6)
2790 GAAGAC
BcgI 1647 CGATGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
344 GCAATACCTTCG
BglI 1199 GCCTACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1354 GCCAGATTGGC
BpuAI 2247 GAAGAC GAAGAC(2/6)
2790 GAAGAC
BseAI 29 TCCGGA T^CCGGA
669 TCCGGA
BsiHKAI 1928 GTGCTC G[AT]GC[AT]^C
2227 GAGCAC
BspEI 29 TCCGGA T^CCGGA
669 TCCGGA
CfoI 1304 GCGC GCG^C
1369 GCGC
HhaI 1304 GCGC GCG^C
1369 GCGC
HinP1I 1304 GCGC G^CGC
1369 GCGC
MaeII 635 ACGT A^CGT
1598 ACGT
MroI 29 TCCGGA T^CCGGA
669 TCCGGA
NcoI 644 CCATGG C^CATGG
1816 CCATGG
NspI 125 ACATGT [GA]CATG^[CT]
356 GCATGT
PaeR7I 854 CTCGAG C^TCGAG
1784 CTCGAG
StuI 1559 AGGCCT AGG^CCT
2101 AGGCCT
XhoI 854 CTCGAG C^TCGAG
1784 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII