(Circular) (Six-base) MAPSORT of: pFN21ASDA1709.seq Check: 7070 from: 1 to: 7804 pFN21ASDA1709 7804 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3685 3685 Size: 7804 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5614 278 Size: 53 83 186 936 4078 2468 Fragments arranged by size: 4078 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5147 1937 Size: 3210 4594 Acc65I G'GTAC_C Cuts at: 5125 5125 Size: 7804 AclI AA'CG_TT Cuts at: 1563 5931 6304 1563 Size: 4368 373 3063 Fragments arranged by size: 4368 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 3549 3609 3807 4289 4508 4907 5105 Size: 534 1383 60 198 482 219 399 198 Cuts at: 5105 5867 7251 7519 1632 Size: 762 1384 268 1917 Fragments arranged by size: 1917 1384 1383 762 534 482 399 268 219 198 198 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7607 AflIII A'CryG_T Cuts at: 1203 2148 6703 7680 1203 Size: 945 4555 977 1327 Fragments arranged by size: 4555 1327 977 945 AgeI A'CCGG_T Cuts at: 6635 6635 Size: 7804 AhdI GACnn_n'nnGTC Cuts at: 6533 6533 Size: 7804 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7804 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 6586 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3885 3917 3885 Size: 32 7772 AlwNI CAG_nnn'CTG Cuts at: 3149 3964 5547 7119 3149 Size: 815 1583 1572 3834 Fragments arranged by size: 3834 1583 1572 815 ApaLI G'TGCA_C Cuts at: 5861 7017 5861 Size: 1156 6648 ApoI r'AATT_y Cuts at: 1493 2819 2993 3071 4029 4850 5113 5339 5491 Size: 1326 174 78 958 821 263 226 152 Cuts at: 5491 7600 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1326 958 821 263 226 174 152 78 AseI AT'TA_AT Cuts at: 160 1032 6358 160 Size: 872 5326 1606 Fragments arranged by size: 5326 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7804 AvaI C'yCGr_G Cuts at: 1870 1943 2877 3807 4684 5129 1870 Size: 73 934 930 877 445 4545 Fragments arranged by size: 4545 934 930 877 445 73 BamHI G'GATC_C Cuts at: 5134 5501 5134 Size: 367 7437 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5125 6580 618 Size: 325 217 627 3338 1455 1842 Fragments arranged by size: 3338 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4806 5123 1391 Size: 3415 317 4072 Fragments arranged by size: 4072 3415 317 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4277 4820 928 Size: 971 2378 543 3912 Fragments arranged by size: 3912 2378 971 543 BbvCI CC'TCA_GC Cuts at: 2726 2726 Size: 7804 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2356 2390 3659 3693 5995 6029 1903 Size: 34 419 34 1269 34 2302 34 3678 Fragments arranged by size: 3678 2302 1269 419 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5696 6906 5696 Size: 1210 6594 BclI T'GATC_A Cuts at: 1548 3087 1548 Size: 1539 6265 BfrBI ATG'CAT Cuts at: 2026 2026 Size: 7804 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3228 3383 6415 Size: 107 122 71 631 605 1556 155 3032 Cuts at: 6415 136 Size: 1525 Fragments arranged by size: 3032 1556 1525 631 605 155 122 107 71 BglII A'GATC_T Cuts at: 2090 7799 2090 Size: 5709 2095 BlpI GC'TnA_GC Cuts at: 2079 5221 2079 Size: 3142 4662 Bme1580I G_kGCm'C Cuts at: 1790 3278 3714 5865 7021 1790 Size: 1488 436 2151 1156 2573 Fragments arranged by size: 2573 2151 1488 1156 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7804 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5535 6493 442 Size: 416 53 83 399 24 4118 958 1753 Fragments arranged by size: 4118 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7804 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2851 3581 4699 2851 Size: 730 1118 5956 Fragments arranged by size: 5956 1118 730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 3401 3641 4019 4517 6464 1239 Size: 1502 660 240 378 498 1947 2579 Fragments arranged by size: 2579 1947 1502 660 498 378 240 Bpu10I CC'TnA_GC Cuts at: 2726 4445 2726 Size: 1719 6085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 3281 4034 4166 5929 6794 7092 2477 Size: 804 753 132 1763 865 298 3189 Fragments arranged by size: 3189 1763 865 804 753 298 132 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2342 6467 882 Size: 617 843 4125 2219 Fragments arranged by size: 4125 2219 843 617 BsaAI yAC'GTr Cuts at: 493 1204 3622 5101 5595 493 Size: 711 2418 1479 494 2702 Fragments arranged by size: 2702 2418 1479 711 494 BsaBI GATnn'nnATC Cuts at: 3385 7798 3385 Size: 4413 3391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5611 5993 6628 275 Size: 53 83 186 936 4078 382 635 1451 Fragments arranged by size: 4078 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2052 2226 2692 4244 4710 6237 6635 6909 7056 Size: 174 466 1552 466 1527 398 274 147 Cuts at: 7056 7467 2052 Size: 411 2389 Fragments arranged by size: 2389 1552 1527 466 466 411 398 274 174 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4366 4396 4458 4488 1178 Size: 30 165 30 2963 30 62 30 4494 Fragments arranged by size: 4494 2963 165 62 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2641 2866 4097 4169 4469 4781 1186 Size: 1455 225 1231 72 300 312 4209 Fragments arranged by size: 4209 1455 1231 312 300 225 72 BseYI C'CCAG_C Cuts at: 2209 2757 2918 3157 4434 4582 4958 7007 7503 Size: 548 161 239 1277 148 376 2049 496 Cuts at: 7503 2209 Size: 2510 Fragments arranged by size: 2510 2049 1277 548 496 376 239 161 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5254 6015 7043 1540 Size: 275 186 3253 761 1028 2301 Fragments arranged by size: 3253 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3955 4254 5865 5950 7021 3955 Size: 299 1611 85 1071 4738 Fragments arranged by size: 4738 1611 1071 299 85 BsmI GAATG_Cn' Cuts at: 2244 2524 3730 5315 5408 2244 Size: 280 1206 1585 93 4640 Fragments arranged by size: 4640 1585 1206 280 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3278 3714 3955 4254 4806 5123 5865 Size: 399 1488 436 241 299 552 317 742 Cuts at: 5865 5950 7021 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1488 1071 742 552 436 399 317 299 241 85 BspEI T'CCGG_A Cuts at: 2052 2692 7467 2052 Size: 640 4775 2389 Fragments arranged by size: 4775 2389 640 BspHI T'CATG_A Cuts at: 5693 7423 5693 Size: 1730 6074 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3685 3975 4101 5159 844 Size: 2841 290 126 1058 3489 Fragments arranged by size: 3489 2841 1058 290 126 BsrBI CCG'CTC Cuts at: 1394 1823 2083 5691 1394 Size: 429 260 3608 3507 Fragments arranged by size: 3608 3507 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 3906 6299 6473 65 Size: 1183 2658 2393 174 1396 Fragments arranged by size: 2658 2393 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3282 6448 6635 1700 Size: 110 1472 3166 187 2869 Fragments arranged by size: 3166 2869 1472 187 110 BsrGI T'GTAC_A Cuts at: 96 2502 96 Size: 2406 5398 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7804 BssSI C'ACGA_G Cuts at: 4255 5864 6876 4255 Size: 1609 1012 5183 Fragments arranged by size: 5183 1609 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3964 794 Size: 3170 4634 BstBI TT'CG_AA Cuts at: 2007 6608 2007 Size: 4601 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7804 BstYI r'GATC_y Cuts at: 1972 2090 2358 3865 5134 5501 5886 5903 7355 Size: 118 268 1507 1269 367 385 17 1452 Cuts at: 7355 7441 7453 7799 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1507 1452 1269 385 367 346 268 118 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2849 2849 Size: 7804 BtgI C'CryG_G Cuts at: 513 1065 2667 3839 4918 513 Size: 552 1602 1172 1079 3399 Fragments arranged by size: 3399 1602 1172 1079 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7065 Fragments arranged by size: 7065 683 56 BtsI GCAGTG_nn' Cuts at: 3090 3348 3398 3456 5320 6114 6134 3090 Size: 258 50 58 1864 794 20 4760 Fragments arranged by size: 4760 1864 794 258 58 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7804 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7769 DraI TTT'AAA Cuts at: 3004 5108 3004 Size: 2104 5700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6811 809 Size: 507 5495 1802 Fragments arranged by size: 5495 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 3280 4551 Size: 54 1425 50 275 18 594 856 1271 Cuts at: 4551 5251 6140 7769 8 Size: 700 889 1629 43 Fragments arranged by size: 1629 1425 1271 889 856 700 594 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5251 1537 Size: 275 3439 4090 Fragments arranged by size: 4090 3439 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 3212 3695 4062 4475 4623 4883 4926 Size: 959 900 483 367 413 148 260 43 Cuts at: 4926 5734 1353 Size: 808 3423 Fragments arranged by size: 3423 959 900 808 483 413 367 260 148 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6391 6765 6911 387 Size: 1064 138 4802 374 146 1280 Fragments arranged by size: 4802 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2741 3401 3549 3609 3641 3807 Size: 393 534 575 660 148 60 32 166 Cuts at: 3807 4019 4289 4508 4517 4907 5105 5867 6464 Size: 212 270 219 9 390 198 762 597 Cuts at: 6464 7251 7519 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 762 660 597 575 534 393 390 270 268 219 212 198 166 148 60 32 9 EcoO109I rG'GnC_Cy Cuts at: 2674 3829 4009 4090 4588 2674 Size: 1155 180 81 498 5890 Fragments arranged by size: 5890 1155 498 180 81 EcoRI G'AATT_C Cuts at: 5113 5491 5113 Size: 378 7426 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 7804 FspI TGC'GCA Cuts at: 6310 6310 Size: 7804 HaeII r_GCGC'y Cuts at: 1678 3330 6951 1678 Size: 1652 3621 2531 Fragments arranged by size: 3621 2531 1652 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2692 2724 6445 6477 6519 Size: 32 135 32 1120 32 3721 32 42 Cuts at: 6519 6551 1373 Size: 32 2626 Fragments arranged by size: 3721 2626 1120 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3320 5148 5394 669 Size: 988 281 1382 1828 246 3079 Fragments arranged by size: 3079 1828 1382 988 281 246 HindIII A'AGCT_T Cuts at: 748 3531 748 Size: 2783 5021 HpaI GTT'AAC Cuts at: 5394 5394 Size: 7804 KpnI G_GTAC'C Cuts at: 5129 5129 Size: 7804 MfeI C'AATT_G Cuts at: 2498 5403 2498 Size: 2905 4899 MluI A'CGCG_T Cuts at: 7680 7680 Size: 7804 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3625 4044 4360 6918 7102 1305 Size: 2320 419 316 2558 184 2007 Fragments arranged by size: 2558 2320 2007 419 316 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 10 Size: 54 1425 343 594 5388 Fragments arranged by size: 5388 1425 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2553 4541 5762 6121 6280 518 Size: 648 1218 169 1988 1221 359 159 2042 Fragments arranged by size: 2042 1988 1221 1218 648 359 169 159 MspA1I CmG'CkG Cuts at: 1606 2248 2873 3961 4582 4660 5220 5897 7045 Size: 642 625 1088 621 78 560 677 1148 Cuts at: 7045 7290 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1088 677 642 625 621 560 245 78 NcoI C'CATG_G Cuts at: 513 1065 2667 3839 513 Size: 552 1602 1172 4478 Fragments arranged by size: 4478 1602 1172 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7804 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7804 NotI GC'GGCC_GC Cuts at: 5251 5251 Size: 7804 NsiI A_TGCA'T Cuts at: 2028 2028 Size: 7804 NspI r_CATG'y Cuts at: 1132 1229 2152 2383 5162 6707 1132 Size: 97 923 231 2779 1545 2229 Fragments arranged by size: 2779 2229 1545 923 231 97 PasI CC'CwG_GG Cuts at: 1348 2902 3440 1348 Size: 1554 538 5712 Fragments arranged by size: 5712 1554 538 PciI A'CATG_T Cuts at: 2148 6703 2148 Size: 4555 3249 PflMI CCAn_nnn'nTGG Cuts at: 1108 2764 3875 4696 1108 Size: 1656 1111 821 4216 Fragments arranged by size: 4216 1656 1111 821 PfoI T'CCnGG_A Cuts at: 3417 3417 Size: 7804 PmeI GTTT'AAAC Cuts at: 5108 5108 Size: 7804 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5873 5905 7386 7418 5873 Size: 32 1481 32 6259 Fragments arranged by size: 6259 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3829 4009 4090 4588 3829 Size: 180 81 498 7045 Fragments arranged by size: 7045 498 180 81 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7804 PsiI TTA'TAA Cuts at: 2199 5374 2199 Size: 3175 4629 PspXI vC'TCGA_Gb Cuts at: 2877 2877 Size: 7804 PstI C_TGCA'G Cuts at: 830 2253 2394 2899 3145 3960 3969 4641 5156 Size: 1423 141 505 246 815 9 672 515 Cuts at: 5156 830 Size: 3478 Fragments arranged by size: 3478 1423 815 672 515 505 246 141 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7804 PvuII CAG'CTG Cuts at: 2248 2873 3961 4582 4660 2248 Size: 625 1088 621 78 5392 Fragments arranged by size: 5392 1088 625 621 78 SalI G'TCGA_C Cuts at: 1936 5146 1936 Size: 3210 4594 SapI GCTCTTCn'nnn_ Cuts at: 3212 4623 4883 3212 Size: 1411 260 6133 Fragments arranged by size: 6133 1411 260 SbfI CC_TGCA'GG Cuts at: 5156 5156 Size: 7804 ScaI AGT'ACT Cuts at: 2857 6052 7764 2857 Size: 3195 1712 2897 Fragments arranged by size: 3195 2897 1712 SfcI C'TryA_G Cuts at: 826 1045 2249 2390 2433 2895 3141 3956 3965 Size: 219 1204 141 43 462 246 815 9 Cuts at: 3965 4637 5152 6287 6968 7159 826 Size: 672 515 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 815 681 672 515 462 246 219 191 141 43 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7804 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7804 SmaI CCC'GGG Cuts at: 1872 5131 1872 Size: 3259 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 2877 3296 3807 4013 4181 Size: 197 926 549 385 419 511 206 168 Cuts at: 4181 5908 6809 7071 820 Size: 1727 901 262 1553 Fragments arranged by size: 1727 1553 926 901 549 511 419 385 262 206 197 168 SnaBI TAC'GTA Cuts at: 493 5101 5595 493 Size: 4608 494 2702 Fragments arranged by size: 4608 2702 494 SpeI A'CTAG_T Cuts at: 152 3172 152 Size: 3020 4784 SphI G_CATG'C Cuts at: 1132 5162 1132 Size: 4030 3774 SspI AAT'ATT Cuts at: 5 52 5728 5 Size: 47 5676 2081 Fragments arranged by size: 5676 2081 47 StuI AGG'CCT Cuts at: 1517 3584 4126 1517 Size: 2067 542 5195 Fragments arranged by size: 5195 2067 542 StyI C'CwwG_G Cuts at: 513 1065 2667 3210 3839 5243 513 Size: 552 1602 543 629 1404 3074 Fragments arranged by size: 3074 1602 1404 629 552 543 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2756 2771 3734 3933 4139 5831 5848 Size: 1255 1176 15 963 199 206 1692 17 Cuts at: 5848 6001 6186 6631 325 Size: 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 1176 963 445 206 199 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 2855 3543 Size: 274 80 33 51 1443 525 353 688 Cuts at: 3543 3972 6050 7762 96 Size: 429 2078 1712 138 Fragments arranged by size: 2078 1712 1443 688 525 429 353 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 4458 4490 7764 7796 2138 Size: 32 2288 32 3274 32 2146 Fragments arranged by size: 3274 2288 2146 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3847 1316 Size: 2531 5273 XbaI T'CTAG_A Cuts at: 2794 5140 2794 Size: 2346 5458 XhoI C'TCGA_G Cuts at: 1943 2877 3807 1943 Size: 934 930 5940 Fragments arranged by size: 5940 934 930 XmaI C'CCGG_G Cuts at: 1870 5129 1870 Size: 3259 4545 XmnI GAAnn'nnTTC Cuts at: 4331 5933 4331 Size: 1602 6202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5612 276 Size: 53 83 186 936 4078 2468 Fragments arranged by size: 4078 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI