Restriction map for
pFN21ASDA1709
(Linear) (Six-base) MAPSORT of: KIAA1709.seq Check: 3509 from: 1 to: 3075
KIAA1709.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1662 3075
Size: 1662 1413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1526 1586 1784 2266 2485 2884 3075
Size: 143 1383 60 198 482 219 399 191
Fragments arranged by size:
1383 482 399 219 198 191 143 60
AflIII A'CryG_T
Cuts at: 0 125 3075
Size: 125 2950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 3075
Size: 379 2696
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1862 1894 3075
Size: 1862 32 1181
Fragments arranged by size:
1862 1181 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1126 1941 3075
Size: 1126 815 1134
Fragments arranged by size:
1134 1126 815
ApoI r'AATT_y
Cuts at: 0 796 970 1048 2006 2827 3075
Size: 796 174 78 958 821 248
Fragments arranged by size:
958 821 796 248 174 78
AvaI C'yCGr_G
Cuts at: 0 854 1784 2661 3075
Size: 854 930 877 414
Fragments arranged by size:
930 877 854 414
BanII G_rGCy'C
Cuts at: 0 2783 3075
Size: 2783 292
BbsI GAAGACnn'nnnn_
Cuts at: 0 2254 2797 3075
Size: 2254 543 278
Fragments arranged by size:
2254 543 278
BbvCI CC'TCA_GC
Cuts at: 0 703 3075
Size: 703 2372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 333 367 1636 1670 3075
Size: 333 34 1269 34 1405
Fragments arranged by size:
1405 1269 333 34 34
BclI T'GATC_A
Cuts at: 0 1064 3075
Size: 1064 2011
BfrBI ATG'CAT
Cuts at: 0 3 3075
Size: 3 3072
BglI GCCn_nnn'nGGC
Cuts at: 0 1205 1360 3075
Size: 1205 155 1715
Fragments arranged by size:
1715 1205 155
BglII A'GATC_T
Cuts at: 0 67 3075
Size: 67 3008
BlpI GC'TnA_GC
Cuts at: 0 56 3075
Size: 56 3019
Bme1580I G_kGCm'C
Cuts at: 0 1255 1691 3075
Size: 1255 436 1384
Fragments arranged by size:
1384 1255 436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 828 1558 2676 3075
Size: 828 730 1118 399
Fragments arranged by size:
1118 828 730 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1378 1618 1996 2494 3075
Size: 718 660 240 378 498 581
Fragments arranged by size:
718 660 581 498 378 240
Bpu10I CC'TnA_GC
Cuts at: 0 703 2422 3075
Size: 703 1719 653
Fragments arranged by size:
1719 703 653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1258 2011 2143 3075
Size: 454 804 753 132 932
Fragments arranged by size:
932 804 753 454 132
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 3075
Size: 319 2756
BsaAI yAC'GTr
Cuts at: 0 1599 3075
Size: 1599 1476
BsaBI GATnn'nnATC
Cuts at: 0 1362 3075
Size: 1362 1713
BsaWI w'CCGG_w
Cuts at: 0 29 203 669 2221 2687 3075
Size: 29 174 466 1552 466 388
Fragments arranged by size:
1552 466 466 388 174 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2343 2373 2435 2465 3075
Size: 2343 30 62 30 610
Fragments arranged by size:
2343 610 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 843 2074 2146 2446 2758 3075
Size: 618 225 1231 72 300 312 317
Fragments arranged by size:
1231 618 317 312 300 225 72
BseYI C'CCAG_C
Cuts at: 0 186 734 895 1134 2411 2559 2935 3075
Size: 186 548 161 239 1277 148 376 140
Fragments arranged by size:
1277 548 376 239 186 161 148 140
BsiHKAI G_wGCw'C
Cuts at: 0 1932 2231 3075
Size: 1932 299 844
Fragments arranged by size:
1932 844 299
BsmI GAATG_Cn'
Cuts at: 0 221 501 1707 3075
Size: 221 280 1206 1368
Fragments arranged by size:
1368 1206 280 221
Bsp1286I G_dGCh'C
Cuts at: 0 1255 1691 1932 2231 2783 3075
Size: 1255 436 241 299 552 292
Fragments arranged by size:
1255 552 436 299 292 241
BspEI T'CCGG_A
Cuts at: 0 29 669 3075
Size: 29 640 2406
Fragments arranged by size:
2406 640 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1662 1952 2078 3075
Size: 1662 290 126 997
Fragments arranged by size:
1662 997 290 126
BsrBI CCG'CTC
Cuts at: 0 60 3075
Size: 60 3015
BsrDI GCAATG_nn'
Cuts at: 0 1883 3075
Size: 1883 1192
BsrFI r'CCGG_y
Cuts at: 0 1259 3075
Size: 1259 1816
BsrGI T'GTAC_A
Cuts at: 0 479 3075
Size: 479 2596
BssSI C'ACGA_G
Cuts at: 0 2232 3075
Size: 2232 843
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1941 3075
Size: 1941 1134
BstYI r'GATC_y
Cuts at: 0 67 335 1842 3075
Size: 67 268 1507 1233
Fragments arranged by size:
1507 1233 268 67
Bsu36I CC'TnA_GG
Cuts at: 0 826 3075
Size: 826 2249
BtgI C'CryG_G
Cuts at: 0 644 1816 2895 3075
Size: 644 1172 1079 180
Fragments arranged by size:
1172 1079 644 180
BtsI GCAGTG_nn'
Cuts at: 0 1067 1325 1375 1433 3075
Size: 1067 258 50 58 1642
Fragments arranged by size:
1642 1067 258 58 50
DraI TTT'AAA
Cuts at: 0 981 3075
Size: 981 2094
EaeI y'GGCC_r
Cuts at: 0 401 1257 2528 3075
Size: 401 856 1271 547
Fragments arranged by size:
1271 856 547 401
EarI CTCTTCn'nnn_
Cuts at: 0 289 1189 1672 2039 2452 2600 2860 2903
Size: 289 900 483 367 413 148 260 43
Cuts at: 2903 3075
Size: 172
Fragments arranged by size:
900 483 413 367 289 260 172 148
43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 718 1378 1526 1586 1618 1784 1996
Size: 143 575 660 148 60 32 166 212
Cuts at: 1996 2266 2485 2494 2884 3075
Size: 270 219 9 390 191
Fragments arranged by size:
660 575 390 270 219 212 191 166
148 143 60 32 9
EcoO109I rG'GnC_Cy
Cuts at: 0 651 1806 1986 2067 2565 3075
Size: 651 1155 180 81 498 510
Fragments arranged by size:
1155 651 510 498 180 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 738 3075
Size: 738 2337
HaeII r_GCGC'y
Cuts at: 0 1307 3075
Size: 1307 1768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 669 701 3075
Size: 669 32 2374
Fragments arranged by size:
2374 669 32
HincII GTy'rAC
Cuts at: 0 1297 3075
Size: 1297 1778
HindIII A'AGCT_T
Cuts at: 0 1508 3075
Size: 1508 1567
MfeI C'AATT_G
Cuts at: 0 475 3075
Size: 475 2600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1602 2021 2337 3075
Size: 1602 419 316 738
Fragments arranged by size:
1602 738 419 316
MscI TGG'CCA
Cuts at: 0 403 3075
Size: 403 2672
MslI CAynn'nnrTG
Cuts at: 0 361 530 2518 3075
Size: 361 169 1988 557
Fragments arranged by size:
1988 557 361 169
MspA1I CmG'CkG
Cuts at: 0 225 850 1938 2559 2637 3075
Size: 225 625 1088 621 78 438
Fragments arranged by size:
1088 625 621 438 225 78
NcoI C'CATG_G
Cuts at: 0 644 1816 3075
Size: 644 1172 1259
Fragments arranged by size:
1259 1172 644
NsiI A_TGCA'T
Cuts at: 0 5 3075
Size: 5 3070
NspI r_CATG'y
Cuts at: 0 129 360 3075
Size: 129 231 2715
Fragments arranged by size:
2715 231 129
PasI CC'CwG_GG
Cuts at: 0 879 1417 3075
Size: 879 538 1658
Fragments arranged by size:
1658 879 538
PciI A'CATG_T
Cuts at: 0 125 3075
Size: 125 2950
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1852 2673 3075
Size: 741 1111 821 402
Fragments arranged by size:
1111 821 741 402
PfoI T'CCnGG_A
Cuts at: 0 1394 3075
Size: 1394 1681
PpuMI rG'GwC_Cy
Cuts at: 0 1806 1986 2067 2565 3075
Size: 1806 180 81 498 510
Fragments arranged by size:
1806 510 498 180 81
PsiI TTA'TAA
Cuts at: 0 176 3075
Size: 176 2899
PspXI vC'TCGA_Gb
Cuts at: 0 854 3075
Size: 854 2221
PstI C_TGCA'G
Cuts at: 0 230 371 876 1122 1937 1946 2618 3075
Size: 230 141 505 246 815 9 672 457
Fragments arranged by size:
815 672 505 457 246 230 141 9
PvuII CAG'CTG
Cuts at: 0 225 850 1938 2559 2637 3075
Size: 225 625 1088 621 78 438
Fragments arranged by size:
1088 625 621 438 225 78
SapI GCTCTTCn'nnn_
Cuts at: 0 1189 2600 2860 3075
Size: 1189 1411 260 215
Fragments arranged by size:
1411 1189 260 215
ScaI AGT'ACT
Cuts at: 0 834 3075
Size: 834 2241
SfcI C'TryA_G
Cuts at: 0 226 367 410 872 1118 1933 1942 2614
Size: 226 141 43 462 246 815 9 672
Cuts at: 2614 3075
Size: 461
Fragments arranged by size:
815 672 462 461 246 226 141 43
9
SmlI C'TyrA_G
Cuts at: 0 469 854 1273 1784 1990 2158 3075
Size: 469 385 419 511 206 168 917
Fragments arranged by size:
917 511 469 419 385 206 168
SpeI A'CTAG_T
Cuts at: 0 1149 3075
Size: 1149 1926
StuI AGG'CCT
Cuts at: 0 1561 2103 3075
Size: 1561 542 972
Fragments arranged by size:
1561 972 542
StyI C'CwwG_G
Cuts at: 0 644 1187 1816 3075
Size: 644 543 629 1259
Fragments arranged by size:
1259 644 629 543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 733 748 1711 1910 2116 3075
Size: 733 15 963 199 206 959
Fragments arranged by size:
963 959 733 206 199 15
TatI w'GTAC_w
Cuts at: 0 479 832 1520 1949 3075
Size: 479 353 688 429 1126
Fragments arranged by size:
1126 688 479 429 353
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 2435 2467 3075
Size: 115 32 2288 32 608
Fragments arranged by size:
2288 608 115 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1824 3075
Size: 1824 1251
XbaI T'CTAG_A
Cuts at: 0 771 3075
Size: 771 2304
XhoI C'TCGA_G
Cuts at: 0 854 1784 3075
Size: 854 930 1291
Fragments arranged by size:
1291 930 854
XmnI GAAnn'nnTTC
Cuts at: 0 2308 3075
Size: 2308 767
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApoI AvaI
BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstYI Bsu36I BtgI BtsI DraI EaeI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NsiI NspI PasI PciI
PflMI PfoI PpuMI PsiI PspXI PstI PvuII SapI
ScaI SfcI SmlI SpeI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BmgBI BmrI BmtI BsaHI
BsgI BsiEI BsiWI BsmBI BspHI BssHII BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PmeI PmlI PpiI PshAI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI SwaI XcmI XmaI ZraI