Restriction map for
pFN21ASDA1706
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbsI BcgI BciVI BclI BfrI
BlnI BpuAI BsaBI BsaI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
KpnI Ksp632I KspI MamI MboII
MfeI MunI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PleI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 1554 TCCGGA T^CCGGA
AfaI 198 GTAC GT^AC
AgeI 1271 ACCGGT A^CCGGT
AocI 1380 CCTGAGG CC^T[ACGT]AGG
Asp700I 1497 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 569 GGATCC G^GATCC
BbeI 259 GGCGCC GGCGC^C
BbrPI 404 CACGTG CAC^GTG
BglII 290 AGATCT A^GATCT
BlpI 314 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 314 GCTCAGC GC^T[ACGT]AGC
BsaAI 404 CACGTG [CT]AC^GT[GA]
BseAI 1554 TCCGGA T^CCGGA
BsgI 933 CTGCAC GTGCAG(16/14)
BsiEI 733 CGGCCG CG[GA][CT]^CG
BsmBI 1094 GAGACG CGTCTC(1/5)
BspEI 1554 TCCGGA T^CCGGA
BspHI 440 TCATGA T^CATGA
BsrDI 1364 GCAATG GCAATG(2/0)
BssSI 1699 CACGAG CACGAG(-5/-1)
BstAPI 897 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 263 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1380 CCTGAGG CC^T[ACGT]AGG
CelII 314 GCTCAGC GC^T[ACGT]AGC
DraIII 577 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 733 CGGCCG C^GGCCG
EclXI 733 CGGCCG C^GGCCG
Eco52I 733 CGGCCG C^GGCCG
Eco81I 1380 CCTGAGG CC^T[ACGT]AGG
EcoRV 1375 GATATC GAT^ATC
HaeII 259 GGCGCC [GA]GCGC^[CT]
KasI 259 GGCGCC G^GCGCC
MaeII 405 ACGT A^CGT
MluI 169 ACGCGT A^CGCGT
MroI 1554 TCCGGA T^CCGGA
NaeI 1078 GCCGGC GCC^GGC
NarI 259 GGCGCC GG^CGCC
NgoMIV 1078 GCCGGC G^CCGGC
PflMI 1269 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1271 ACCGGT A^CCGGT
PmaCI 404 CACGTG CAC^GTG
PmlI 404 CACGTG CAC^GTG
PstI 676 CTGCAG CTGCA^G
PvuII 386 CAGCTG CAG^CTG
RcaI 440 TCATGA T^CATGA
RsaI 198 GTAC GT^AC
SacI 1113 GAGCTC GAGCT^C
SexAI 578 ACCAGGT A^CC[AT]GGT
SfoI 259 GGCGCC GGC^GCC
SmlI 1448 CTCAAG C^T[CT][GA]AG
Van91I 1269 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1035 CCAGAAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1497 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 259 GGCGCC G[GA]^CG[CT]C
1642 GACGCC
AflIII 169 ACGCGT A^C[GA][CT]GT
405 ACGTGT
AspHI 6 GAGCAC G[AT]GC[AT]^C
1113 GAGCTC
BanI 259 GGCGCC G^G[CT][GA]CC
575 GGCACC
BbvCI 474 CCTCAGC CCTCAGC(-5/-2)
780 GCTGAGG
BglI 424 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
702 GCCAGGGGGGC
BsaHI 259 GGCGCC G[GA]^CG[CT]C
1642 GACGCC
BsaWI 1271 ACCGGT [AT]^CCGG[AT]
1554 TCCGGA
BseRI 139 GAGGAG GAGGAG(10/8)
142 GAGGAG
BsiHKAI 6 GAGCAC G[AT]GC[AT]^C
1113 GAGCTC
BsmAI 1094 GAGAC GTCTC(1/5)
1461 GTCTC
BsrBI 24 CCGCTC CCGCTC(-3/-3)
112 GAGCGG
BsrFI 1078 GCCGGC [GA]^CCGG[CT]
1271 ACCGGT
BstYI 290 AGATCT [GA]^GATC[CT]
569 GGATCC
Cfr10I 1078 GCCGGC [GA]^CCGG[CT]
1271 ACCGGT
Hin1I 259 GGCGCC G[GA]^CG[CT]C
1642 GACGCC
HincII 409 GTTAAC GT[CT]^[GA]AC
1201 GTTAAC
HindII 409 GTTAAC GT[CT]^[GA]AC
1201 GTTAAC
HindIII 883 AAGCTT A^AGCTT
1488 AAGCTT
HpaI 409 GTTAAC GTT^AAC
1201 GTTAAC
MflI 290 AGATCT [GA]^GATC[CT]
569 GGATCC
MspA1I 386 CAGCTG C[AC]G^C[GT]G
1659 CAGCGG
NheI 354 GCTAGC G^CTAGC
1602 GCTAGC
SfcI 676 CTGCAG C^T[GA][CT]AG
1297 CTACAG
SmaI 835 CCCGGG CCC^GGG
986 CCCGGG
TaqI 1024 TCGA T^CGA
1456 TCGA
TthHB8I 1024 TCGA T^CGA
1456 TCGA
XhoII 290 AGATCT [GA]^GATC[CT]
569 GGATCC
XmaCI 835 CCCGGG C^CCGGG
986 CCCGGG
XmaI 835 CCCGGG C^CCGGG
986 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI Sau3AI Sau96I ScrFI SfaNI
StuI StyI TfiI Tru9I TseI
Tsp45I TspRI