(Circular) (Six-base) MAPSORT of: pFN21ASDA1706.seq Check: 2955 from: 1 to: 6436 pFN21ASDA1706 6436 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3779 1937 Size: 1842 4594 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 4499 5883 6151 1632 Size: 463 2404 1384 268 1917 Fragments arranged by size: 2404 1917 1384 463 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 2192 2428 5335 6312 1203 Size: 989 236 2907 977 1327 Fragments arranged by size: 2907 1327 989 977 236 AgeI A'CCGG_T Cuts at: 3294 5267 3294 Size: 1973 4463 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 2411 1166 Size: 1245 5191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 4179 5751 4179 Size: 1572 4864 ApaI G_GGCC'C Cuts at: 2532 2736 3009 3173 2532 Size: 204 273 164 5795 Fragments arranged by size: 5795 273 204 164 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 3745 3971 4123 6232 1493 Size: 2252 226 152 2109 1697 Fragments arranged by size: 2252 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2677 2858 3009 3185 3761 1870 Size: 73 734 181 151 176 576 4545 Fragments arranged by size: 4545 734 576 181 176 151 73 BamHI G'GATC_C Cuts at: 2592 3766 4133 2592 Size: 1174 367 4895 Fragments arranged by size: 4895 1174 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2598 3757 5212 618 Size: 325 217 627 495 316 1159 1455 1842 Fragments arranged by size: 1842 1455 1159 627 495 325 316 217 BanII G_rGCy'C Cuts at: 1391 2532 2684 2736 3009 3140 3173 3755 1391 Size: 1141 152 52 273 131 33 582 4072 Fragments arranged by size: 4072 1141 582 273 152 131 52 33 BbeI G_GCGC'C Cuts at: 2286 2286 Size: 6436 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5465 BbvCI CC'TCA_GC Cuts at: 2498 2804 2498 Size: 306 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4627 4661 1903 Size: 34 2690 34 3678 Fragments arranged by size: 3678 2690 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 2731 5047 Size: 107 122 71 631 605 781 278 2316 Cuts at: 5047 136 Size: 1525 Fragments arranged by size: 2316 1525 781 631 605 278 122 107 71 BglII A'GATC_T Cuts at: 2313 6431 2313 Size: 4118 2318 BlpI GC'TnA_GC Cuts at: 2338 3853 2338 Size: 1515 4921 Bme1580I G_kGCm'C Cuts at: 1790 2418 2532 2736 3009 3156 3173 4497 5653 Size: 628 114 204 273 147 17 1324 1156 Cuts at: 5653 1790 Size: 2573 Fragments arranged by size: 2573 1324 1156 628 273 204 147 114 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 3547 3614 Size: 416 53 83 399 24 932 1198 67 Cuts at: 3614 4167 5125 442 Size: 553 958 1753 Fragments arranged by size: 1753 1198 958 932 553 416 399 83 67 53 24 BmtI G_CTAG'C Cuts at: 1056 2381 3629 1056 Size: 1325 1248 3863 Fragments arranged by size: 3863 1325 1248 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 3575 3061 Size: 514 5922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 3041 3673 5096 1239 Size: 1076 726 632 1423 2579 Fragments arranged by size: 2579 1423 1076 726 632 Bpu10I CC'TnA_GC Cuts at: 2498 2693 2804 2498 Size: 195 111 6130 Fragments arranged by size: 6130 195 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3456 4561 5426 5724 3456 Size: 1105 865 298 4168 Fragments arranged by size: 4168 1105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5099 882 Size: 617 3600 2219 Fragments arranged by size: 3600 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2429 3733 4227 493 Size: 711 1225 1304 494 2702 Fragments arranged by size: 2702 1304 1225 711 494 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 3666 4243 4625 Size: 53 83 186 936 750 1383 577 382 Cuts at: 4625 5260 275 Size: 635 1451 Fragments arranged by size: 1451 1383 936 750 635 577 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3294 3577 4869 5267 5541 5688 6099 3294 Size: 283 1292 398 274 147 411 3631 Fragments arranged by size: 3631 1292 411 398 283 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6211 Fragments arranged by size: 6211 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2180 1186 Size: 991 3 5442 Fragments arranged by size: 5442 991 3 BseYI C'CCAG_C Cuts at: 2457 3068 3173 3269 5639 6135 2457 Size: 611 105 96 2370 496 2758 Fragments arranged by size: 2758 2370 611 496 105 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2941 1163 Size: 573 1205 4658 Fragments arranged by size: 4658 1205 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2759 3886 4647 5675 1540 Size: 275 186 758 1127 761 1028 2301 Fragments arranged by size: 2301 1127 1028 761 758 275 186 BsiHKAI G_wGCw'C Cuts at: 2033 3140 4497 4582 5653 2033 Size: 1107 1357 85 1071 2816 Fragments arranged by size: 2816 1357 1107 1071 85 BsmI GAATG_Cn' Cuts at: 3947 4040 3947 Size: 93 6343 BsmBI CGTCTCn'nnnn_ Cuts at: 3111 3111 Size: 6436 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2418 2532 2684 2736 3009 3140 Size: 399 243 385 114 152 52 273 131 Cuts at: 3140 3156 3173 3755 4497 4582 5653 1391 Size: 16 17 582 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 399 385 273 243 152 131 114 85 52 17 16 BspEI T'CCGG_A Cuts at: 3577 6099 3577 Size: 2522 3914 BspHI T'CATG_A Cuts at: 2463 4325 6055 2463 Size: 1862 1730 2844 Fragments arranged by size: 2844 1862 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 2382 2727 3791 844 Size: 1446 92 345 1064 3489 Fragments arranged by size: 3489 1446 1064 345 92 BsrBI CCG'CTC Cuts at: 1394 1823 2049 2137 4323 1394 Size: 429 226 88 2186 3507 Fragments arranged by size: 3507 2186 429 226 88 BsrDI GCAATG_nn' Cuts at: 65 1248 3394 4931 5105 65 Size: 1183 2146 1537 174 1396 Fragments arranged by size: 2146 1537 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3101 3294 5080 5267 1700 Size: 110 1291 193 1786 187 2869 Fragments arranged by size: 2869 1786 1291 193 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 3722 4496 5508 3722 Size: 774 1012 4650 Fragments arranged by size: 4650 1012 774 BstAPI GCAn_nnn'nTGC Cuts at: 794 2926 794 Size: 2132 4304 BstBI TT'CG_AA Cuts at: 2007 5240 2007 Size: 3233 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2293 Size: 6436 BstYI r'GATC_y Cuts at: 1972 2313 2592 3766 4133 4518 4535 5987 6073 Size: 341 279 1174 367 385 17 1452 86 Cuts at: 6073 6085 6431 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1174 385 367 346 341 279 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3404 3404 Size: 6436 BtgI C'CryG_G Cuts at: 513 1065 2022 2157 2199 2763 2838 513 Size: 552 957 135 42 564 75 4111 Fragments arranged by size: 4111 957 564 552 135 75 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5697 Fragments arranged by size: 5697 683 56 BtsI GCAGTG_nn' Cuts at: 2151 3613 3952 4746 4766 2151 Size: 1462 339 794 20 3821 Fragments arranged by size: 3821 1462 794 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DraIII CAC_nnn'GTG Cuts at: 2605 2605 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 2756 2811 Size: 54 1425 50 275 18 573 353 55 Cuts at: 2811 3289 3370 3883 4772 6401 8 Size: 478 81 513 889 1629 43 Fragments arranged by size: 1629 1425 889 573 513 478 353 275 81 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2756 3883 1537 Size: 275 944 1127 4090 Fragments arranged by size: 4090 1127 944 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4366 1353 Size: 3013 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2504 5023 5397 5543 387 Size: 1064 138 915 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 915 374 146 138 EcoICRI GAG'CTC Cuts at: 3138 3138 Size: 6436 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2315 3041 3673 4499 5096 5883 Size: 393 463 220 726 632 826 597 787 Cuts at: 5883 6151 1239 Size: 268 1524 Fragments arranged by size: 1524 826 787 726 632 597 463 393 268 220 EcoO109I rG'GnC_Cy Cuts at: 2062 2257 2371 2529 2548 2625 2710 2732 3005 Size: 195 114 158 19 77 85 22 273 Cuts at: 3005 3006 3170 2062 Size: 1 164 5328 Fragments arranged by size: 5328 273 195 164 158 114 85 77 22 19 1 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 EcoRV GAT'ATC Cuts at: 3400 3400 Size: 6436 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2945 2945 Size: 6436 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 2286 5583 1678 Size: 608 3297 2531 Fragments arranged by size: 3297 2531 608 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 3250 3282 3572 Size: 32 135 32 645 32 1001 32 290 Cuts at: 3572 3604 5077 5109 5151 5183 1373 Size: 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1473 1001 645 290 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 3226 3780 4026 669 Size: 988 281 496 792 554 246 3079 Fragments arranged by size: 3079 988 792 554 496 281 246 HindIII A'AGCT_T Cuts at: 748 2906 3511 748 Size: 2158 605 3673 Fragments arranged by size: 3673 2158 605 HpaI GTT'AAC Cuts at: 2434 3226 4026 2434 Size: 792 800 4844 Fragments arranged by size: 4844 800 792 KasI G'GCGC_C Cuts at: 2282 2282 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 2192 6312 2192 Size: 4120 2316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5550 5734 1305 Size: 4245 184 2007 Fragments arranged by size: 4245 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 2813 3291 3372 10 Size: 54 1425 343 573 408 478 81 3074 Fragments arranged by size: 3074 1425 573 478 408 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2411 2468 3285 4394 4753 4912 Size: 648 957 288 57 817 1109 359 159 Cuts at: 4912 518 Size: 2042 Fragments arranged by size: 2042 1109 957 817 648 359 288 159 57 MspA1I CmG'CkG Cuts at: 1606 2411 3684 3852 4529 5677 5922 1606 Size: 805 1273 168 677 1148 245 2120 Fragments arranged by size: 2120 1273 1148 805 677 245 168 NaeI GCC'GGC Cuts at: 3103 3103 Size: 6436 NarI GG'CG_CC Cuts at: 2283 2283 Size: 6436 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4927 Fragments arranged by size: 4927 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NgoMIV G'CCGG_C Cuts at: 3101 3101 Size: 6436 NheI G'CTAG_C Cuts at: 1052 2377 3625 1052 Size: 1325 1248 3863 Fragments arranged by size: 3863 1325 1248 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 3794 5339 1132 Size: 97 2565 1545 2229 Fragments arranged by size: 2565 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2035 2058 1348 Size: 687 23 5726 Fragments arranged by size: 5726 687 23 PciI A'CATG_T Cuts at: 5335 5335 Size: 6436 PflMI CCAn_nnn'nTGG Cuts at: 1108 3298 1108 Size: 2190 4246 PfoI T'CCnGG_A Cuts at: 2583 2889 2583 Size: 306 6130 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PmlI CAC'GTG Cuts at: 2429 2429 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2257 2371 2548 2710 2062 Size: 195 114 177 162 5788 Fragments arranged by size: 5788 195 177 162 114 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PspOMI G'GGCC_C Cuts at: 2528 2732 3005 3169 2528 Size: 204 273 164 5795 Fragments arranged by size: 5795 273 204 164 PstI C_TGCA'G Cuts at: 830 2703 3788 830 Size: 1873 1085 3478 Fragments arranged by size: 3478 1873 1085 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 2411 2411 Size: 6436 SacI G_AGCT'C Cuts at: 3140 3140 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SanDI GG'GwC_CC Cuts at: 2062 2062 Size: 6436 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SexAI A'CCwGG_T Cuts at: 2601 2601 Size: 6436 SfcI C'TryA_G Cuts at: 826 1045 2699 3320 3784 4919 5600 5791 826 Size: 219 1654 621 464 1135 681 191 1471 Fragments arranged by size: 1654 1471 1135 681 621 464 219 191 SfoI GGC'GCC Cuts at: 2284 2284 Size: 6436 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 2860 3011 3763 1872 Size: 988 151 752 4545 Fragments arranged by size: 4545 988 752 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 3471 4540 5441 5703 820 Size: 197 926 1528 1069 901 262 1553 Fragments arranged by size: 1553 1528 1069 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3733 4227 493 Size: 3240 494 2702 Fragments arranged by size: 3240 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 2478 2771 2797 2942 3558 1517 Size: 961 293 26 145 616 4395 Fragments arranged by size: 4395 961 616 293 145 26 StyI C'CwwG_G Cuts at: 513 1065 2022 2943 3373 3569 3693 3875 513 Size: 552 957 921 430 196 124 182 3074 Fragments arranged by size: 3074 957 921 552 430 196 182 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 2753 4463 4480 4633 4818 5263 Size: 1255 1071 102 1710 17 153 185 445 Cuts at: 5263 325 Size: 1498 Fragments arranged by size: 1710 1498 1255 1071 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4682 6394 96 Size: 274 80 33 51 1443 2705 1712 138 Fragments arranged by size: 2705 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6396 6428 6396 Size: 32 6404 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3065 3065 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 2858 3009 3761 1870 Size: 988 151 752 4545 Fragments arranged by size: 4545 988 752 151 XmnI GAAnn'nnTTC Cuts at: 3524 4565 3524 Size: 1041 5395 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI