Restriction map for
pFN21ASDA1706
(Linear) (Six-base) MAPSORT of: KIAA1706.seq Check: 674 from: 1 to: 1707
KIAA1706.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1707
Size: 72 1635
AflIII A'CryG_T
Cuts at: 0 169 405 1707
Size: 169 236 1302
Fragments arranged by size:
1302 236 169
AgeI A'CCGG_T
Cuts at: 0 1271 1707
Size: 1271 436
AleI CACnn'nnGTG
Cuts at: 0 388 1707
Size: 388 1319
ApaI G_GGCC'C
Cuts at: 0 509 713 986 1150 1707
Size: 509 204 273 164 557
Fragments arranged by size:
557 509 273 204 164
AvaI C'yCGr_G
Cuts at: 0 654 835 986 1162 1707
Size: 654 181 151 176 545
Fragments arranged by size:
654 545 181 176 151
BamHI G'GATC_C
Cuts at: 0 569 1707
Size: 569 1138
BanI G'GyrC_C
Cuts at: 0 259 575 1707
Size: 259 316 1132
Fragments arranged by size:
1132 316 259
BanII G_rGCy'C
Cuts at: 0 509 661 713 986 1117 1150 1707
Size: 509 152 52 273 131 33 557
Fragments arranged by size:
557 509 273 152 131 52 33
BbeI G_GCGC'C
Cuts at: 0 263 1707
Size: 263 1444
BbvCI CC'TCA_GC
Cuts at: 0 475 781 1707
Size: 475 306 926
Fragments arranged by size:
926 475 306
BglI GCCn_nnn'nGGC
Cuts at: 0 430 708 1707
Size: 430 278 999
Fragments arranged by size:
999 430 278
BglII A'GATC_T
Cuts at: 0 290 1707
Size: 290 1417
BlpI GC'TnA_GC
Cuts at: 0 315 1707
Size: 315 1392
Bme1580I G_kGCm'C
Cuts at: 0 395 509 713 986 1133 1150 1707
Size: 395 114 204 273 147 17 557
Fragments arranged by size:
557 395 273 204 147 114 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 326 1524 1591 1707
Size: 326 1198 67 116
Fragments arranged by size:
1198 326 116 67
BmtI G_CTAG'C
Cuts at: 0 358 1606 1707
Size: 358 1248 101
Fragments arranged by size:
1248 358 101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1038 1552 1707
Size: 1038 514 155
Fragments arranged by size:
1038 514 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1018 1650 1707
Size: 292 726 632 57
Fragments arranged by size:
726 632 292 57
Bpu10I CC'TnA_GC
Cuts at: 0 475 670 781 1707
Size: 475 195 111 926
Fragments arranged by size:
926 475 195 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1433 1707
Size: 1433 274
BsaAI yAC'GTr
Cuts at: 0 406 1707
Size: 406 1301
BsaHI Gr'CG_yC
Cuts at: 0 260 1643 1707
Size: 260 1383 64
Fragments arranged by size:
1383 260 64
BsaWI w'CCGG_w
Cuts at: 0 1271 1554 1707
Size: 1271 283 153
Fragments arranged by size:
1271 283 153
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 157 1707
Size: 154 3 1550
Fragments arranged by size:
1550 154 3
BseYI C'CCAG_C
Cuts at: 0 434 1045 1150 1246 1707
Size: 434 611 105 96 461
Fragments arranged by size:
611 461 434 105 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1707
Size: 918 789
BsiEI CG_ry'CG
Cuts at: 0 736 1707
Size: 736 971
BsiHKAI G_wGCw'C
Cuts at: 0 10 1117 1707
Size: 10 1107 590
Fragments arranged by size:
1107 590 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1088 1707
Size: 1088 619
Bsp1286I G_dGCh'C
Cuts at: 0 10 395 509 661 713 986 1117 1133
Size: 10 385 114 152 52 273 131 16
Cuts at: 1133 1150 1707
Size: 17 557
Fragments arranged by size:
557 385 273 152 131 114 52 17
16 10
BspEI T'CCGG_A
Cuts at: 0 1554 1707
Size: 1554 153
BspHI T'CATG_A
Cuts at: 0 440 1707
Size: 440 1267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 267 359 704 1707
Size: 267 92 345 1003
Fragments arranged by size:
1003 345 267 92
BsrBI CCG'CTC
Cuts at: 0 26 114 1707
Size: 26 88 1593
Fragments arranged by size:
1593 88 26
BsrDI GCAATG_nn'
Cuts at: 0 1371 1707
Size: 1371 336
BsrFI r'CCGG_y
Cuts at: 0 1078 1271 1707
Size: 1078 193 436
Fragments arranged by size:
1078 436 193
BssSI C'ACGA_G
Cuts at: 0 1699 1707
Size: 1699 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 903 1707
Size: 903 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 1707
Size: 270 1437
BstYI r'GATC_y
Cuts at: 0 290 569 1707
Size: 290 279 1138
Fragments arranged by size:
1138 290 279
Bsu36I CC'TnA_GG
Cuts at: 0 1381 1707
Size: 1381 326
BtgI C'CryG_G
Cuts at: 0 134 176 740 815 1707
Size: 134 42 564 75 892
Fragments arranged by size:
892 564 134 75 42
BtsI GCAGTG_nn'
Cuts at: 0 128 1590 1707
Size: 128 1462 117
Fragments arranged by size:
1462 128 117
DraIII CAC_nnn'GTG
Cuts at: 0 582 1707
Size: 582 1125
EaeI y'GGCC_r
Cuts at: 0 380 733 788 1266 1347 1707
Size: 380 353 55 478 81 360
Fragments arranged by size:
478 380 360 353 81 55
EagI C'GGCC_G
Cuts at: 0 733 1707
Size: 733 974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 481 1707
Size: 481 1226
EcoICRI GAG'CTC
Cuts at: 0 1115 1707
Size: 1115 592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 292 1018 1650 1707
Size: 72 220 726 632 57
Fragments arranged by size:
726 632 220 72 57
EcoO109I rG'GnC_Cy
Cuts at: 0 39 234 348 506 525 602 687 709
Size: 39 195 114 158 19 77 85 22
Cuts at: 709 982 983 1147 1707
Size: 273 1 164 560
Fragments arranged by size:
560 273 195 164 158 114 85 77
39 22 19 1
EcoRV GAT'ATC
Cuts at: 0 1377 1707
Size: 1377 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 922 1707
Size: 922 785
HaeII r_GCGC'y
Cuts at: 0 263 1707
Size: 263 1444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 1227 1259 1549 1581 1707
Size: 194 32 1001 32 290 32 126
Fragments arranged by size:
1001 290 194 126 32 32 32
HincII GTy'rAC
Cuts at: 0 411 1203 1707
Size: 411 792 504
Fragments arranged by size:
792 504 411
HindIII A'AGCT_T
Cuts at: 0 883 1488 1707
Size: 883 605 219
Fragments arranged by size:
883 605 219
HpaI GTT'AAC
Cuts at: 0 411 1203 1707
Size: 411 792 504
Fragments arranged by size:
792 504 411
KasI G'GCGC_C
Cuts at: 0 259 1707
Size: 259 1448
MluI A'CGCG_T
Cuts at: 0 169 1707
Size: 169 1538
MscI TGG'CCA
Cuts at: 0 382 790 1268 1349 1707
Size: 382 408 478 81 358
Fragments arranged by size:
478 408 382 358 81
MslI CAynn'nnrTG
Cuts at: 0 100 388 445 1262 1707
Size: 100 288 57 817 445
Fragments arranged by size:
817 445 288 100 57
MspA1I CmG'CkG
Cuts at: 0 388 1661 1707
Size: 388 1273 46
Fragments arranged by size:
1273 388 46
NaeI GCC'GGC
Cuts at: 0 1080 1707
Size: 1080 627
NarI GG'CG_CC
Cuts at: 0 260 1707
Size: 260 1447
NgoMIV G'CCGG_C
Cuts at: 0 1078 1707
Size: 1078 629
NheI G'CTAG_C
Cuts at: 0 354 1602 1707
Size: 354 1248 105
Fragments arranged by size:
1248 354 105
PasI CC'CwG_GG
Cuts at: 0 12 35 1707
Size: 12 23 1672
Fragments arranged by size:
1672 23 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 1275 1707
Size: 1275 432
PfoI T'CCnGG_A
Cuts at: 0 560 866 1707
Size: 560 306 841
Fragments arranged by size:
841 560 306
PmlI CAC'GTG
Cuts at: 0 406 1707
Size: 406 1301
PpuMI rG'GwC_Cy
Cuts at: 0 39 234 348 525 687 1707
Size: 39 195 114 177 162 1020
Fragments arranged by size:
1020 195 177 162 114 39
PspOMI G'GGCC_C
Cuts at: 0 505 709 982 1146 1707
Size: 505 204 273 164 561
Fragments arranged by size:
561 505 273 204 164
PstI C_TGCA'G
Cuts at: 0 680 1707
Size: 680 1027
PvuII CAG'CTG
Cuts at: 0 388 1707
Size: 388 1319
SacI G_AGCT'C
Cuts at: 0 1117 1707
Size: 1117 590
SanDI GG'GwC_CC
Cuts at: 0 39 1707
Size: 39 1668
SexAI A'CCwGG_T
Cuts at: 0 578 1707
Size: 578 1129
SfcI C'TryA_G
Cuts at: 0 676 1297 1707
Size: 676 621 410
Fragments arranged by size:
676 621 410
SfoI GGC'GCC
Cuts at: 0 261 1707
Size: 261 1446
SmaI CCC'GGG
Cuts at: 0 837 988 1707
Size: 837 151 719
Fragments arranged by size:
837 719 151
SmlI C'TyrA_G
Cuts at: 0 1448 1707
Size: 1448 259
StuI AGG'CCT
Cuts at: 0 455 748 774 919 1535 1707
Size: 455 293 26 145 616 172
Fragments arranged by size:
616 455 293 172 145 26
StyI C'CwwG_G
Cuts at: 0 920 1350 1546 1670 1707
Size: 920 430 196 124 37
Fragments arranged by size:
920 430 196 124 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 628 730 1707
Size: 628 102 977
Fragments arranged by size:
977 628 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1042 1707
Size: 1042 665
XmaI C'CCGG_G
Cuts at: 0 835 986 1707
Size: 835 151 721
Fragments arranged by size:
835 721 151
XmnI GAAnn'nnTTC
Cuts at: 0 1501 1707
Size: 1501 206
Enzymes that do cut:
AcuI AflIII AgeI AleI ApaI AvaI BamHI BanI
BanII BbeI BbvCI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EciI
EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII HpaI KasI MluI MscI MslI MspA1I NaeI
NarI NgoMIV NheI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SanDI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BciVI BclI BfrBI BmgBI
BsaI BsaBI BsaXI BsiWI BsmI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EarI
EcoNI EcoRI FseI FspI FspAI KpnI MfeI MmeI
NcoI NdeI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI ZraI