(Circular) (Six-base) MAPSORT of: pFN21ASDA1701.seq Check: 1539 from: 1 to: 6370 pFN21ASDA1701 6370 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2914 4180 278 Size: 53 83 186 936 1378 1266 2468 Fragments arranged by size: 2468 1378 1266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 4433 5817 6085 1632 Size: 1167 1634 1384 268 1917 Fragments arranged by size: 1917 1634 1384 1167 268 AflII C'TTAA_G Cuts at: 820 1017 3017 820 Size: 197 2000 4173 Fragments arranged by size: 4173 2000 197 AflIII A'CryG_T Cuts at: 1203 2321 3232 5269 6246 1203 Size: 1118 911 2037 977 1327 Fragments arranged by size: 2037 1327 1118 977 911 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2830 1184 Size: 1646 4724 AlwNI CAG_nnn'CTG Cuts at: 2516 4113 5685 2516 Size: 1597 1572 3201 Fragments arranged by size: 3201 1597 1572 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 2993 3679 3905 4057 6166 1493 Size: 1500 686 226 152 2109 1697 Fragments arranged by size: 2109 1697 1500 686 226 152 AseI AT'TA_AT Cuts at: 160 1032 2637 4924 160 Size: 872 1605 2287 1606 Fragments arranged by size: 2287 1606 1605 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 3695 1870 Size: 73 1752 4545 Fragments arranged by size: 4545 1752 73 AvrII C'CTAG_G Cuts at: 2504 2504 Size: 6370 BamHI G'GATC_C Cuts at: 3700 4067 3700 Size: 367 6003 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 2731 3691 5146 618 Size: 325 217 627 663 281 960 1455 1842 Fragments arranged by size: 1842 1455 960 663 627 325 281 217 BanII G_rGCy'C Cuts at: 1391 2053 2245 3689 1391 Size: 662 192 1444 4072 Fragments arranged by size: 4072 1444 662 192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5399 BbvCI CC'TCA_GC Cuts at: 2466 2517 2619 2466 Size: 51 102 6217 Fragments arranged by size: 6217 102 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4262 5472 4262 Size: 1210 5160 BclI T'GATC_A Cuts at: 1548 3465 3598 1548 Size: 1917 133 4320 Fragments arranged by size: 4320 1917 133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4981 136 Size: 107 122 71 631 605 3309 1525 Fragments arranged by size: 3309 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6365 6365 Size: 6370 BlpI GC'TnA_GC Cuts at: 3787 3787 Size: 6370 Bme1580I G_kGCm'C Cuts at: 1790 2455 4431 5587 1790 Size: 665 1976 1156 2573 Fragments arranged by size: 2573 1976 1156 665 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4101 5059 442 Size: 416 53 83 399 24 2684 958 1753 Fragments arranged by size: 2684 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2688 5030 1239 Size: 876 573 2342 2579 Fragments arranged by size: 2579 2342 876 573 Bpu10I CC'TnA_GC Cuts at: 2466 2517 2619 2466 Size: 51 102 6217 Fragments arranged by size: 6217 102 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3625 4495 5360 5658 3625 Size: 870 865 298 4337 Fragments arranged by size: 4337 870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5033 882 Size: 617 3534 2219 Fragments arranged by size: 3534 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3667 4161 493 Size: 711 2463 494 2702 Fragments arranged by size: 2702 2463 711 494 BsaBI GATnn'nnATC Cuts at: 2722 6364 2722 Size: 3642 2728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2911 4177 4559 5194 Size: 53 83 186 936 1378 1266 382 635 Cuts at: 5194 275 Size: 1451 Fragments arranged by size: 1451 1378 1266 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4803 5201 5475 5622 6033 4803 Size: 398 274 147 411 5140 Fragments arranged by size: 5140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6145 Fragments arranged by size: 6145 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2534 2537 2540 1186 Size: 1297 51 3 3 5016 Fragments arranged by size: 5016 1297 51 3 3 BseYI C'CCAG_C Cuts at: 2027 2297 2360 2486 5573 6069 2027 Size: 270 63 126 3087 496 2328 Fragments arranged by size: 3087 2328 496 270 126 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5797 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3820 4581 5609 1540 Size: 275 186 1819 761 1028 2301 Fragments arranged by size: 2301 1819 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2245 4431 4516 5587 2245 Size: 2186 85 1071 3028 Fragments arranged by size: 3028 2186 1071 85 BsmI GAATG_Cn' Cuts at: 2978 3881 3974 2978 Size: 903 93 5374 Fragments arranged by size: 5374 903 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2222 2222 Size: 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2245 2455 3689 4431 4516 5587 Size: 399 263 192 210 1234 742 85 1071 Cuts at: 5587 1391 Size: 2174 Fragments arranged by size: 2174 1234 1071 742 399 263 210 192 85 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3725 844 Size: 1644 1237 3489 Fragments arranged by size: 3489 1644 1237 BsrBI CCG'CTC Cuts at: 1394 1823 4257 1394 Size: 429 2434 3507 Fragments arranged by size: 3507 2434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 4865 5039 65 Size: 1183 1821 1796 174 1396 Fragments arranged by size: 1821 1796 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5014 5201 1700 Size: 110 3204 187 2869 Fragments arranged by size: 3204 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 3059 794 Size: 2265 4105 BstBI TT'CG_AA Cuts at: 2007 5174 2007 Size: 3167 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstYI r'GATC_y Cuts at: 1972 2723 3700 4067 4452 4469 5921 6007 6019 Size: 751 977 367 385 17 1452 86 12 Cuts at: 6019 6365 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 977 751 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3385 3385 Size: 6370 BtgI C'CryG_G Cuts at: 513 1065 2022 2274 513 Size: 552 957 252 4609 Fragments arranged by size: 4609 957 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 2208 3886 4680 4700 2208 Size: 1678 794 20 3878 Fragments arranged by size: 3878 1678 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3429 3674 3429 Size: 245 6125 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5377 809 Size: 507 4061 1802 Fragments arranged by size: 4061 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3543 3624 3817 Size: 54 1425 50 275 18 1713 81 193 Cuts at: 3817 4706 6335 8 Size: 889 1629 43 Fragments arranged by size: 1713 1629 1425 889 275 193 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3817 1537 Size: 275 2005 4090 Fragments arranged by size: 4090 2005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2361 2397 2526 4300 1353 Size: 1008 36 129 1774 3423 Fragments arranged by size: 3423 1774 1008 129 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4957 5331 5477 387 Size: 1064 138 3368 374 146 1280 Fragments arranged by size: 3368 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2243 2243 Size: 6370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2688 2799 4433 5030 5817 6085 Size: 393 483 573 111 1634 597 787 268 Cuts at: 6085 1239 Size: 1524 Fragments arranged by size: 1634 1524 787 597 573 483 393 268 111 EcoNI CCTnn'n_nnAGG Cuts at: 2508 2508 Size: 6370 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2769 3545 2769 Size: 776 5594 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 5517 1678 Size: 3839 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2896 2901 2928 2933 3450 Size: 32 135 32 1324 5 27 5 517 Cuts at: 3450 3482 5011 5043 5085 5117 1373 Size: 32 1529 32 42 32 2626 Fragments arranged by size: 2626 1529 1324 517 135 42 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3714 3960 669 Size: 988 281 1086 690 246 3079 Fragments arranged by size: 3079 1086 988 690 281 246 HindIII A'AGCT_T Cuts at: 748 3136 748 Size: 2388 3982 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3585 3969 3585 Size: 384 5986 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2454 3470 5484 5668 1305 Size: 1149 1016 2014 184 2007 Fragments arranged by size: 2014 2007 1149 1016 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3545 3626 10 Size: 54 1425 343 1713 81 2754 Fragments arranged by size: 2754 1713 1425 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 4328 4687 4846 518 Size: 648 3162 359 159 2042 Fragments arranged by size: 3162 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2708 2777 3786 4463 5611 5856 1606 Size: 1102 69 1009 677 1148 245 2120 Fragments arranged by size: 2120 1148 1102 1009 677 245 69 NcoI C'CATG_G Cuts at: 513 1065 2022 2274 513 Size: 552 957 252 4609 Fragments arranged by size: 4609 957 552 252 NdeI CA'TA_TG Cuts at: 387 3243 387 Size: 2856 3514 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 2325 3236 3728 5273 1132 Size: 97 1096 911 492 1545 2229 Fragments arranged by size: 2229 1545 1096 911 492 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6370 PciI A'CATG_T Cuts at: 2321 3232 5269 2321 Size: 911 2037 3422 Fragments arranged by size: 3422 2037 911 PflMI CCAn_nnn'nTGG Cuts at: 1108 2661 1108 Size: 1553 4817 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2822 2854 4439 4471 5952 5984 2822 Size: 32 1585 32 1481 32 3208 Fragments arranged by size: 3208 1585 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3612 3940 3612 Size: 328 6042 PstI C_TGCA'G Cuts at: 830 3454 3722 830 Size: 2624 268 3478 Fragments arranged by size: 3478 2624 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2708 2708 Size: 6370 SacI G_AGCT'C Cuts at: 2245 2245 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 2130 3450 3718 4853 5534 5725 826 Size: 219 1085 1320 268 1135 681 191 1471 Fragments arranged by size: 1471 1320 1135 1085 681 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 3697 1872 Size: 1825 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3017 3604 4474 5375 5637 820 Size: 197 926 1074 587 870 901 262 1553 Fragments arranged by size: 1553 1074 926 901 870 587 262 197 SnaBI TAC'GTA Cuts at: 493 3667 4161 493 Size: 3174 494 2702 Fragments arranged by size: 3174 2702 494 SpeI A'CTAG_T Cuts at: 152 2573 152 Size: 2421 3949 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2128 1517 Size: 611 5759 StyI C'CwwG_G Cuts at: 513 1065 2022 2274 2292 2504 2655 3809 513 Size: 552 957 252 18 212 151 1154 3074 Fragments arranged by size: 3074 1154 957 552 252 212 151 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3056 3099 4397 4414 4567 4752 5197 Size: 1255 1476 43 1298 17 153 185 445 Cuts at: 5197 325 Size: 1498 Fragments arranged by size: 1498 1476 1298 1255 445 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4616 6328 96 Size: 274 80 33 51 1443 2639 1712 138 Fragments arranged by size: 2639 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2838 2870 6330 6362 2838 Size: 32 3460 32 2846 Fragments arranged by size: 3460 2846 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2167 1316 Size: 851 5519 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 3695 1870 Size: 1825 4545 XmnI GAAnn'nnTTC Cuts at: 4499 4499 Size: 6370 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2912 4178 276 Size: 53 83 186 936 1378 1266 2468 Fragments arranged by size: 2468 1378 1266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI