(Circular) (Six-base) MAPSORT of: pFN21ASDA1696.seq Check: 4116 from: 1 to: 6631 pFN21ASDA1696 6631 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3088 3088 Size: 6631 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2570 4441 278 Size: 53 83 186 936 1034 1871 2468 Fragments arranged by size: 2468 1871 1034 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3222 3786 3974 1937 Size: 1285 564 188 4594 Fragments arranged by size: 4594 1285 564 188 Acc65I G'GTAC_C Cuts at: 3952 3952 Size: 6631 AclI AA'CG_TT Cuts at: 1563 2915 4758 5131 1563 Size: 1352 1843 373 3063 Fragments arranged by size: 3063 1843 1352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3515 4694 6078 6346 1632 Size: 1883 1179 1384 268 1917 Fragments arranged by size: 1917 1883 1384 1179 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6434 AflIII A'CryG_T Cuts at: 1203 3398 5530 6507 1203 Size: 2195 2132 977 1327 Fragments arranged by size: 2195 2132 1327 977 AgeI A'CCGG_T Cuts at: 5462 5462 Size: 6631 AhdI GACnn_n'nnGTC Cuts at: 5360 5360 Size: 6631 AleI CACnn'nnGTG Cuts at: 1166 2537 1166 Size: 1371 5260 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2367 2555 1184 Size: 1183 188 5260 Fragments arranged by size: 5260 1183 188 AlwNI CAG_nnn'CTG Cuts at: 2080 2447 2600 3385 3493 4374 5946 2080 Size: 367 153 785 108 881 1572 2765 Fragments arranged by size: 2765 1572 881 785 367 153 108 ApaLI G'TGCA_C Cuts at: 4688 5844 4688 Size: 1156 5475 ApoI r'AATT_y Cuts at: 1493 2061 2860 3940 4166 4318 6427 1493 Size: 568 799 1080 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 799 568 226 152 AseI AT'TA_AT Cuts at: 160 1032 5185 160 Size: 872 4153 1606 Fragments arranged by size: 4153 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 AvaI C'yCGr_G Cuts at: 1870 1943 3956 1870 Size: 73 2013 4545 Fragments arranged by size: 4545 2013 73 BamHI G'GATC_C Cuts at: 2101 3961 4328 2101 Size: 1860 367 4404 Fragments arranged by size: 4404 1860 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3952 5407 618 Size: 325 217 627 2165 1455 1842 Fragments arranged by size: 2165 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3950 1391 Size: 2559 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2390 928 Size: 971 491 5169 Fragments arranged by size: 5169 971 491 BbvCI CC'TCA_GC Cuts at: 2655 2796 2655 Size: 141 6490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4822 4856 1903 Size: 34 2885 34 3678 Fragments arranged by size: 3678 2885 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4523 5733 4523 Size: 1210 5421 BclI T'GATC_A Cuts at: 1548 1548 Size: 6631 BfrBI ATG'CAT Cuts at: 3675 3675 Size: 6631 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5242 136 Size: 107 122 71 631 605 3570 1525 Fragments arranged by size: 3570 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6626 6626 Size: 6631 BlpI GC'TnA_GC Cuts at: 2528 2993 3653 4048 2528 Size: 465 660 395 5111 Fragments arranged by size: 5111 660 465 395 Bme1580I G_kGCm'C Cuts at: 1790 4692 5848 1790 Size: 2902 1156 2573 Fragments arranged by size: 2902 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2705 1318 Size: 1387 5244 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2592 4362 5320 Size: 416 53 83 399 24 1175 1770 958 Cuts at: 5320 442 Size: 1753 Fragments arranged by size: 1770 1753 1175 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6631 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 2940 3749 5291 1239 Size: 1307 394 809 1542 2579 Fragments arranged by size: 2579 1542 1307 809 394 Bpu10I CC'TnA_GC Cuts at: 2655 2796 2655 Size: 141 6490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4756 5621 5919 4756 Size: 865 298 5468 Fragments arranged by size: 5468 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2473 5294 882 Size: 617 974 2821 2219 Fragments arranged by size: 2821 2219 974 617 BsaAI yAC'GTr Cuts at: 493 1204 3928 4422 493 Size: 711 2724 494 2702 Fragments arranged by size: 2724 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6625 6625 Size: 6631 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2567 4438 4820 5455 Size: 53 83 186 936 1034 1871 382 635 Cuts at: 5455 275 Size: 1451 Fragments arranged by size: 1871 1451 1034 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2227 3217 5064 5462 5736 5883 6294 2227 Size: 990 1847 398 274 147 411 2564 Fragments arranged by size: 2564 1847 990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6406 Fragments arranged by size: 6406 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2643 2784 3119 3868 1186 Size: 1457 141 335 749 3949 Fragments arranged by size: 3949 1457 749 335 141 BseYI C'CCAG_C Cuts at: 2340 2767 2928 5834 6330 2340 Size: 427 161 2906 496 2641 Fragments arranged by size: 2906 2641 496 427 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2561 3069 3282 3814 3874 1163 Size: 573 825 508 213 532 60 3920 Fragments arranged by size: 3920 825 573 532 508 213 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4081 4842 5870 1540 Size: 275 186 2080 761 1028 2301 Fragments arranged by size: 2301 2080 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 4692 4777 5848 2529 Size: 2163 85 1071 3312 Fragments arranged by size: 3312 2163 1071 85 BsmI GAATG_Cn' Cuts at: 2116 4142 4235 2116 Size: 2026 93 4512 Fragments arranged by size: 4512 2026 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 3950 4692 4777 5848 1391 Size: 399 739 1421 742 85 1071 2174 Fragments arranged by size: 2174 1421 1071 742 739 399 85 BspEI T'CCGG_A Cuts at: 6294 6294 Size: 6631 BspHI T'CATG_A Cuts at: 2134 3343 4520 6250 2134 Size: 1209 1177 1730 2515 Fragments arranged by size: 2515 1730 1209 1177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2626 3088 3986 844 Size: 1782 462 898 3489 Fragments arranged by size: 3489 1782 898 462 BsrBI CCG'CTC Cuts at: 1394 1823 4518 1394 Size: 429 2695 3507 Fragments arranged by size: 3507 2695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2249 2487 5126 5300 65 Size: 1183 1001 238 2639 174 1396 Fragments arranged by size: 2639 1396 1183 1001 238 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3862 5275 5462 1700 Size: 110 2052 1413 187 2869 Fragments arranged by size: 2869 2052 1413 187 110 BsrGI T'GTAC_A Cuts at: 96 2986 96 Size: 2890 3741 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6631 BssSI C'ACGA_G Cuts at: 4691 5703 4691 Size: 1012 5619 BstAPI GCAn_nnn'nTGC Cuts at: 794 2447 3363 794 Size: 1653 916 4062 Fragments arranged by size: 4062 1653 916 BstBI TT'CG_AA Cuts at: 2007 5435 2007 Size: 3428 3203 BstEII G'GTnAC_C Cuts at: 1184 2497 1184 Size: 1313 5318 BstXI CCAn_nnnn'nTGG Cuts at: 2494 2740 2494 Size: 246 6385 BstYI r'GATC_y Cuts at: 1972 2101 3465 3961 4328 4713 4730 6182 6268 Size: 129 1364 496 367 385 17 1452 86 Cuts at: 6268 6280 6626 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1364 496 385 367 346 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2922 3515 3760 513 Size: 552 957 900 593 245 3384 Fragments arranged by size: 3384 957 900 593 552 245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5892 Fragments arranged by size: 5892 683 56 BtsI GCAGTG_nn' Cuts at: 3270 4147 4941 4961 3270 Size: 877 794 20 4940 Fragments arranged by size: 4940 877 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6596 DraI TTT'AAA Cuts at: 3612 3935 3612 Size: 323 6308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5638 809 Size: 507 4322 1802 Fragments arranged by size: 4322 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3326 3376 4078 Size: 54 1425 50 275 18 1496 50 702 Cuts at: 4078 4967 6596 8 Size: 889 1629 43 Fragments arranged by size: 1629 1496 1425 889 702 275 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4078 1537 Size: 275 2266 4090 Fragments arranged by size: 4090 2266 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3251 3299 3570 3909 4561 1353 Size: 1898 48 271 339 652 3423 Fragments arranged by size: 3423 1898 652 339 271 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5218 5592 5738 387 Size: 1064 138 3629 374 146 1280 Fragments arranged by size: 3629 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2546 2940 3515 3749 4694 5291 6078 Size: 393 914 394 575 234 945 597 787 Cuts at: 6078 6346 1239 Size: 268 1524 Fragments arranged by size: 1524 945 914 787 597 575 394 393 268 234 EcoNI CCTnn'n_nnAGG Cuts at: 3731 3731 Size: 6631 EcoO109I rG'GnC_Cy Cuts at: 3297 3297 Size: 6631 EcoRI G'AATT_C Cuts at: 2860 3940 4318 2860 Size: 1080 378 5173 Fragments arranged by size: 5173 1080 378 FspI TGC'GCA Cuts at: 5137 5137 Size: 6631 HaeII r_GCGC'y Cuts at: 1678 5778 1678 Size: 4100 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 2938 2970 5272 Size: 32 135 32 838 32 496 32 2302 Cuts at: 5272 5304 5346 5378 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2302 838 496 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2557 3975 4221 669 Size: 988 281 619 1418 246 3079 Fragments arranged by size: 3079 1418 988 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6631 HpaI GTT'AAC Cuts at: 4221 4221 Size: 6631 KpnI G_GTAC'C Cuts at: 3956 3956 Size: 6631 MfeI C'AATT_G Cuts at: 3534 4230 3534 Size: 696 5935 MluI A'CGCG_T Cuts at: 6507 6507 Size: 6631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5745 5929 1305 Size: 4440 184 2007 Fragments arranged by size: 4440 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3328 3378 10 Size: 54 1425 343 1496 50 3263 Fragments arranged by size: 3263 1496 1425 343 54 50 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2537 3680 4589 4948 5107 518 Size: 648 1326 45 1143 909 359 159 2042 Fragments arranged by size: 2042 1326 1143 909 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2351 3745 4047 4724 5872 6117 1606 Size: 745 1394 302 677 1148 245 2120 Fragments arranged by size: 2120 1394 1148 745 677 302 245 NaeI GCC'GGC Cuts at: 3864 3864 Size: 6631 NcoI C'CATG_G Cuts at: 513 1065 2022 2922 3515 513 Size: 552 957 900 593 3629 Fragments arranged by size: 3629 957 900 593 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6631 NgoMIV G'CCGG_C Cuts at: 3862 3862 Size: 6631 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6631 NotI GC'GGCC_GC Cuts at: 4078 4078 Size: 6631 NsiI A_TGCA'T Cuts at: 3677 3677 Size: 6631 NspI r_CATG'y Cuts at: 1132 1229 3402 3463 3989 5534 1132 Size: 97 2173 61 526 1545 2229 Fragments arranged by size: 2229 2173 1545 526 97 61 PasI CC'CwG_GG Cuts at: 1348 3293 1348 Size: 1945 4686 PciI A'CATG_T Cuts at: 3398 5530 3398 Size: 2132 4499 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 2611 3521 1108 Size: 972 531 910 4218 Fragments arranged by size: 4218 972 910 531 PmeI GTTT'AAAC Cuts at: 3935 3935 Size: 6631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4700 4732 6213 6245 4700 Size: 32 1481 32 5086 Fragments arranged by size: 5086 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3297 3297 Size: 6631 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6631 PsiI TTA'TAA Cuts at: 4201 4201 Size: 6631 PstI C_TGCA'G Cuts at: 830 2356 2637 3983 830 Size: 1526 281 1346 3478 Fragments arranged by size: 3478 1526 1346 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6631 PvuII CAG'CTG Cuts at: 2351 3745 2351 Size: 1394 5237 SalI G'TCGA_C Cuts at: 1936 3973 1936 Size: 2037 4594 SanDI GG'GwC_CC Cuts at: 3297 3297 Size: 6631 SbfI CC_TGCA'GG Cuts at: 3983 3983 Size: 6631 ScaI AGT'ACT Cuts at: 4879 6591 4879 Size: 1712 4919 SfcI C'TryA_G Cuts at: 826 1045 2039 2297 2352 2394 2633 3223 3783 Size: 219 994 258 55 42 239 590 560 Cuts at: 3783 3979 5114 5795 5986 826 Size: 196 1135 681 191 1471 Fragments arranged by size: 1471 1135 994 681 590 560 258 239 219 196 191 55 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6631 SmaI CCC'GGG Cuts at: 1872 3958 1872 Size: 2086 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4735 5636 5898 820 Size: 197 926 2792 901 262 1553 Fragments arranged by size: 2792 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3928 4422 493 Size: 3435 494 2702 Fragments arranged by size: 3435 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6631 SphI G_CATG'C Cuts at: 1132 3989 1132 Size: 2857 3774 SspI AAT'ATT Cuts at: 5 52 4555 5 Size: 47 4503 2081 Fragments arranged by size: 4503 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6631 StyI C'CwwG_G Cuts at: 513 1065 2022 2922 3453 3515 4070 513 Size: 552 957 900 531 62 555 3074 Fragments arranged by size: 3074 957 900 555 552 531 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3103 4658 4675 4828 5013 5458 325 Size: 1255 1523 1555 17 153 185 445 1498 Fragments arranged by size: 1555 1523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2986 4877 6589 Size: 274 80 33 51 1443 1009 1891 1712 Cuts at: 6589 96 Size: 138 Fragments arranged by size: 1891 1712 1443 1009 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2664 2689 2696 2721 2830 2862 6591 6623 2664 Size: 25 7 25 109 32 3729 32 2672 Fragments arranged by size: 3729 2672 109 32 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6631 XbaI T'CTAG_A Cuts at: 3163 3967 3163 Size: 804 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6631 XmaI C'CCGG_G Cuts at: 1870 3956 1870 Size: 2086 4545 XmnI GAAnn'nnTTC Cuts at: 3510 4760 3510 Size: 1250 5381 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2568 4439 276 Size: 53 83 186 936 1034 1871 2468 Fragments arranged by size: 2468 1871 1034 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI