(Circular) (Six-base) MAPSORT of: pFN21ASDA1691.seq Check: 6545 from: 1 to: 6298 pFN21ASDA1691 6298 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2614 2614 Size: 6298 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2685 4108 278 Size: 53 83 186 936 1149 1423 2468 Fragments arranged by size: 2468 1423 1149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3641 1937 Size: 1704 4594 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4361 5745 6013 1632 Size: 2729 1384 268 1917 Fragments arranged by size: 2729 1917 1384 268 AfeI AGC'GCT Cuts at: 3150 3150 Size: 6298 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 2306 2441 2597 3083 1166 Size: 1140 135 156 486 4381 Fragments arranged by size: 4381 1140 486 156 135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6298 AlwNI CAG_nnn'CTG Cuts at: 2149 2482 2533 2542 2953 4041 5613 2149 Size: 333 51 9 411 1088 1572 2834 Fragments arranged by size: 2834 1572 1088 411 333 51 9 ApaLI G'TGCA_C Cuts at: 4355 5511 4355 Size: 1156 5142 ApoI r'AATT_y Cuts at: 1493 3607 3833 3985 6094 1493 Size: 2114 226 152 2109 1697 Fragments arranged by size: 2114 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2507 3623 1870 Size: 73 180 384 1116 4545 Fragments arranged by size: 4545 1116 384 180 73 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 3619 5074 618 Size: 325 217 627 257 1575 1455 1842 Fragments arranged by size: 1842 1575 1455 627 325 257 217 BanII G_rGCy'C Cuts at: 1391 2469 2514 2822 3617 1391 Size: 1078 45 308 795 4072 Fragments arranged by size: 4072 1078 795 308 45 BbeI G_GCGC'C Cuts at: 2048 2048 Size: 6298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5327 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 1548 Size: 6298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 4909 136 Size: 107 122 71 631 605 502 2735 1525 Fragments arranged by size: 2735 1525 631 605 502 122 107 71 BglII A'GATC_T Cuts at: 6293 6293 Size: 6298 BlpI GC'TnA_GC Cuts at: 3000 3715 3000 Size: 715 5583 Bme1580I G_kGCm'C Cuts at: 1790 4359 5515 1790 Size: 2569 1156 2573 Fragments arranged by size: 2573 2569 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4029 4987 442 Size: 416 53 83 399 24 2612 958 1753 Fragments arranged by size: 2612 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2893 3582 2893 Size: 689 5609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2567 2627 2806 3291 3514 3562 4958 Size: 1265 63 60 179 485 223 48 1396 Cuts at: 4958 1239 Size: 2579 Fragments arranged by size: 2579 1396 1265 485 223 179 63 60 48 Bpu10I CC'TnA_GC Cuts at: 2090 2090 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4423 5288 5586 4423 Size: 865 298 5135 Fragments arranged by size: 5135 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2345 3383 4961 882 Size: 617 846 1038 1578 2219 Fragments arranged by size: 2219 1578 1038 846 617 BsaAI yAC'GTr Cuts at: 493 1204 2854 3142 3595 4089 493 Size: 711 1650 288 453 494 2702 Fragments arranged by size: 2702 1650 711 494 453 288 BsaBI GATnn'nnATC Cuts at: 6292 6292 Size: 6298 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 2262 2682 2847 Size: 53 83 186 936 512 217 420 165 Cuts at: 2847 4105 4487 5122 275 Size: 1258 382 635 1451 Fragments arranged by size: 1451 1258 936 635 512 420 382 217 186 165 83 53 BsaWI w'CCGG_w Cuts at: 4731 5129 5403 5550 5961 4731 Size: 398 274 147 411 5068 Fragments arranged by size: 5068 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6073 Fragments arranged by size: 6073 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2264 2885 3041 3308 3311 1186 Size: 1000 78 621 156 267 3 4173 Fragments arranged by size: 4173 1000 621 267 156 78 3 BseYI C'CCAG_C Cuts at: 2094 2468 2557 2653 2755 2781 2791 3353 5501 Size: 374 89 96 102 26 10 562 2148 Cuts at: 5501 5997 2094 Size: 496 2395 Fragments arranged by size: 2395 2148 562 496 374 102 96 89 26 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2260 2332 1163 Size: 573 315 209 72 5129 Fragments arranged by size: 5129 573 315 209 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2571 3405 3748 4509 5537 1540 Size: 275 186 570 834 343 761 1028 2301 Fragments arranged by size: 2301 1028 834 761 570 343 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 4359 4444 5515 2256 Size: 2103 85 1071 3039 Fragments arranged by size: 3039 2103 1071 85 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 BsmBI CGTCTCn'nnnn_ Cuts at: 2543 2543 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2469 2514 2822 3617 4359 4444 Size: 399 466 213 45 308 795 742 85 Cuts at: 4444 5515 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 795 742 466 399 308 213 85 45 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2614 3099 3653 844 Size: 1770 485 554 3489 Fragments arranged by size: 3489 1770 554 485 BsrBI CCG'CTC Cuts at: 1394 1823 2488 3305 4185 1394 Size: 429 665 817 880 3507 Fragments arranged by size: 3507 880 817 665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4793 4967 65 Size: 1183 3545 174 1396 Fragments arranged by size: 3545 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 2232 2318 3031 3155 4942 5129 Size: 110 260 162 86 713 124 1787 187 Cuts at: 5129 1700 Size: 2869 Fragments arranged by size: 2869 1787 713 260 187 162 124 110 86 BsrGI T'GTAC_A Cuts at: 96 3360 96 Size: 3264 3034 BssHII G'CGCG_C Cuts at: 1925 2787 1925 Size: 862 5436 BssSI C'ACGA_G Cuts at: 3556 4358 5370 3556 Size: 802 1012 4484 Fragments arranged by size: 4484 1012 802 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 2149 2542 794 Size: 1280 75 393 4550 Fragments arranged by size: 4550 1280 393 75 BstBI TT'CG_AA Cuts at: 2007 5102 2007 Size: 3095 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6298 BstXI CCAn_nnnn'nTGG Cuts at: 2866 2866 Size: 6298 BstYI r'GATC_y Cuts at: 1972 2629 3628 3995 4380 4397 5849 5935 5947 Size: 657 999 367 385 17 1452 86 12 Cuts at: 5947 6293 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 999 657 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2814 3314 3405 513 Size: 552 957 792 500 91 3406 Fragments arranged by size: 3406 957 792 552 500 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2281 2308 487 Size: 683 56 1055 27 4477 Fragments arranged by size: 4477 1055 683 56 27 BtsI GCAGTG_nn' Cuts at: 2334 3391 3814 4608 4628 2334 Size: 1057 423 794 20 4004 Fragments arranged by size: 4004 1057 794 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2409 2496 Size: 54 1425 50 275 18 195 384 87 Cuts at: 2496 2568 3033 3402 3745 4634 6263 8 Size: 72 465 369 343 889 1629 43 Fragments arranged by size: 1629 1425 889 465 384 369 343 275 195 87 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2568 3402 3745 1537 Size: 275 756 834 343 4090 Fragments arranged by size: 4090 834 756 343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 2213 3206 4228 1353 Size: 851 9 993 1022 3423 Fragments arranged by size: 3423 1022 993 851 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2378 2477 2633 4885 5259 5405 Size: 1064 138 789 99 156 2252 374 146 Cuts at: 5405 387 Size: 1280 Fragments arranged by size: 2252 1280 1064 789 374 156 146 138 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 2567 2627 2806 3291 3514 3562 Size: 393 872 63 60 179 485 223 48 Cuts at: 3562 4361 4958 5745 6013 1239 Size: 799 597 787 268 1524 Fragments arranged by size: 1524 872 799 787 597 485 393 268 223 179 63 60 48 EcoNI CCTnn'n_nnAGG Cuts at: 2584 2584 Size: 6298 EcoO109I rG'GnC_Cy Cuts at: 2146 2539 3043 3181 3275 2146 Size: 393 504 138 94 5169 Fragments arranged by size: 5169 504 393 138 94 EcoRI G'AATT_C Cuts at: 3607 3985 3607 Size: 378 5920 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 2048 2319 3152 5445 1678 Size: 370 271 833 2293 2531 Fragments arranged by size: 2531 2293 833 370 271 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4939 4971 5013 5045 1373 Size: 32 135 32 3367 32 42 32 2626 Fragments arranged by size: 3367 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3888 669 Size: 988 281 1704 246 3079 Fragments arranged by size: 3079 1704 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 2044 2044 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5412 5596 1305 Size: 4107 184 2007 Fragments arranged by size: 4107 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4476 Fragments arranged by size: 4476 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2327 2441 2597 2738 3083 4256 Size: 648 1140 21 114 156 141 345 1173 Cuts at: 4256 4615 4774 518 Size: 359 159 2042 Fragments arranged by size: 2042 1173 1140 648 359 345 159 156 141 114 21 MspA1I CmG'CkG Cuts at: 1606 2494 2781 3316 3366 3407 3477 3582 3714 Size: 888 287 535 50 41 70 105 132 Cuts at: 3714 4391 5539 5784 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 677 535 287 245 132 105 70 50 41 NaeI GCC'GGC Cuts at: 2234 2320 3033 2234 Size: 86 713 5499 Fragments arranged by size: 5499 713 86 NarI GG'CG_CC Cuts at: 2045 2045 Size: 6298 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4789 Fragments arranged by size: 4789 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NgoMIV G'CCGG_C Cuts at: 2232 2318 3031 2232 Size: 86 713 5499 Fragments arranged by size: 5499 713 86 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 3352 3656 5201 1132 Size: 97 2123 304 1545 2229 Fragments arranged by size: 2229 2123 1545 304 97 PasI CC'CwG_GG Cuts at: 1348 3021 1348 Size: 1673 4625 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 3497 1108 Size: 2389 3909 PfoI T'CCnGG_A Cuts at: 2418 3060 2418 Size: 642 5656 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3043 3043 Size: 6298 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PstI C_TGCA'G Cuts at: 830 2478 2538 2904 3096 3245 3650 830 Size: 1648 60 366 192 149 405 3478 Fragments arranged by size: 3478 1648 405 366 192 149 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 2494 2781 3366 2494 Size: 287 585 5426 Fragments arranged by size: 5426 585 287 SacII CC_GC'GG Cuts at: 3317 3408 3317 Size: 91 6207 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SapI GCTCTTCn'nnn_ Cuts at: 2213 2213 Size: 6298 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 2876 2906 4546 6258 2876 Size: 30 1640 1712 2916 Fragments arranged by size: 2916 1712 1640 30 SfcI C'TryA_G Cuts at: 826 1045 2474 2534 2900 3092 3241 3646 4781 Size: 219 1429 60 366 192 149 405 1135 Cuts at: 4781 5462 5653 826 Size: 681 191 1471 Fragments arranged by size: 1471 1429 1135 681 405 366 219 192 191 149 60 SfoI GGC'GCC Cuts at: 2046 2046 Size: 6298 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4402 5303 5565 820 Size: 197 926 2459 901 262 1553 Fragments arranged by size: 2459 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3595 4089 493 Size: 3102 494 2702 Fragments arranged by size: 3102 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6298 StyI C'CwwG_G Cuts at: 513 1065 2022 2727 3737 513 Size: 552 957 705 1010 3074 Fragments arranged by size: 3074 1010 957 705 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 4325 4342 4495 4680 5125 325 Size: 1255 464 2281 17 153 185 445 1498 Fragments arranged by size: 2281 1498 1255 464 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2874 2904 3360 Size: 274 80 33 51 1443 897 30 456 Cuts at: 3360 3441 4544 6256 96 Size: 81 1103 1712 138 Fragments arranged by size: 1712 1443 1103 897 456 274 138 81 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 6258 6290 2414 Size: 32 3812 32 2422 Fragments arranged by size: 3812 2422 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6298 XbaI T'CTAG_A Cuts at: 3634 3634 Size: 6298 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2404 2734 2404 Size: 330 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 4427 4427 Size: 6298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2683 4106 276 Size: 53 83 186 936 1149 1423 2468 Fragments arranged by size: 2468 1423 1149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI