(Circular) (Six-base) MAPSORT of: pFN21ASDA1688.seq Check: 3968 from: 1 to: 7978 pFN21ASDA1688 7978 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4849 4849 Size: 7978 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5788 278 Size: 53 83 186 936 4252 2468 Fragments arranged by size: 4252 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2064 3219 5321 1937 Size: 127 1155 2102 4594 Fragments arranged by size: 4594 2102 1155 127 Acc65I G'GTAC_C Cuts at: 4929 5299 4929 Size: 370 7608 AclI AA'CG_TT Cuts at: 1563 6105 6478 1563 Size: 4542 373 3063 Fragments arranged by size: 4542 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 2870 3814 4643 4798 4871 4940 6041 Size: 737 501 944 829 155 73 69 1101 Cuts at: 6041 7425 7693 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1101 944 829 737 501 268 155 73 69 AfeI AGC'GCT Cuts at: 3164 4727 3164 Size: 1563 6415 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7781 AflIII A'CryG_T Cuts at: 1203 2250 6877 7854 1203 Size: 1047 4627 977 1327 Fragments arranged by size: 4627 1327 1047 977 AgeI A'CCGG_T Cuts at: 2165 6809 2165 Size: 4644 3334 AhdI GACnn_n'nnGTC Cuts at: 3466 6707 3466 Size: 3241 4737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4712 1184 Size: 3528 4450 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 4100 4132 2348 Size: 32 1720 32 6194 Fragments arranged by size: 6194 1720 32 32 AlwNI CAG_nnn'CTG Cuts at: 2347 3133 3321 3343 3586 3870 3913 4360 5242 Size: 786 188 22 243 284 43 447 882 Cuts at: 5242 5721 7293 2347 Size: 479 1572 3032 Fragments arranged by size: 3032 1572 882 786 479 447 284 243 188 43 22 ApaI G_GGCC'C Cuts at: 3113 3560 3113 Size: 447 7531 ApaLI G'TGCA_C Cuts at: 5021 6035 7191 5021 Size: 1014 1156 5808 Fragments arranged by size: 5808 1156 1014 ApoI r'AATT_y Cuts at: 1493 5287 5513 5665 7774 1493 Size: 3794 226 152 2109 1697 Fragments arranged by size: 3794 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6532 160 Size: 872 5500 1606 Fragments arranged by size: 5500 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7978 AvaI C'yCGr_G Cuts at: 1870 1943 2393 2447 2641 3158 3487 3560 3809 Size: 73 450 54 194 517 329 73 249 Cuts at: 3809 4733 5000 5303 1870 Size: 924 267 303 4545 Fragments arranged by size: 4545 924 517 450 329 303 267 249 194 73 73 54 BamHI G'GATC_C Cuts at: 5308 5675 5308 Size: 367 7611 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2298 2645 2810 3788 3881 Size: 325 217 627 511 347 165 978 93 Cuts at: 3881 4876 4929 5146 5299 6754 618 Size: 995 53 217 153 1455 1842 Fragments arranged by size: 1842 1455 995 978 627 511 347 325 217 217 165 153 93 53 BanII G_rGCy'C Cuts at: 1391 2448 2454 2688 2865 2970 3006 3113 3239 Size: 1057 6 234 177 105 36 107 126 Cuts at: 3239 3560 3678 3963 4260 4374 4443 4950 5297 Size: 321 118 285 297 114 69 507 347 Cuts at: 5297 1391 Size: 4072 Fragments arranged by size: 4072 1057 507 347 321 297 285 234 177 126 118 114 107 105 69 36 6 BbeI G_GCGC'C Cuts at: 2649 3792 5150 2649 Size: 1143 1358 5477 Fragments arranged by size: 5477 1358 1143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4051 4899 5074 928 Size: 971 2152 848 175 3832 Fragments arranged by size: 3832 2152 971 848 175 BbvCI CC'TCA_GC Cuts at: 3329 3329 Size: 7978 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4826 4860 6169 6203 1903 Size: 34 2889 34 1309 34 3678 Fragments arranged by size: 3678 2889 1309 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5870 7080 5870 Size: 1210 6768 BclI T'GATC_A Cuts at: 1548 1548 Size: 7978 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2421 2801 3656 Size: 107 122 71 631 605 749 380 855 Cuts at: 3656 3707 4035 6589 136 Size: 51 328 2554 1525 Fragments arranged by size: 2554 1525 855 749 631 605 380 328 122 107 71 51 BglII A'GATC_T Cuts at: 7973 7973 Size: 7978 BlpI GC'TnA_GC Cuts at: 5395 5395 Size: 7978 Bme1580I G_kGCm'C Cuts at: 1790 3113 3494 3560 3884 4006 4881 5025 6039 Size: 1323 381 66 324 122 875 144 1014 Cuts at: 6039 7195 1790 Size: 1156 2573 Fragments arranged by size: 2573 1323 1156 1014 875 381 324 144 122 66 BmgBI CAC'GTC Cuts at: 1318 2253 3016 4903 1318 Size: 935 763 1887 4393 Fragments arranged by size: 4393 1887 935 763 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2966 4000 4120 Size: 416 53 83 399 24 1549 1034 120 Cuts at: 4120 5709 6667 442 Size: 1589 958 1753 Fragments arranged by size: 1753 1589 1549 1034 958 416 399 120 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7978 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 3077 3197 3223 3892 3938 4033 4207 Size: 1223 615 120 26 669 46 95 174 Cuts at: 4207 4346 4366 4436 4961 6638 1239 Size: 139 20 70 525 1677 2579 Fragments arranged by size: 2579 1677 1223 669 615 525 174 139 120 95 70 46 26 20 Bpu10I CC'TnA_GC Cuts at: 3170 3329 3384 3170 Size: 159 55 7764 Fragments arranged by size: 7764 159 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 6103 6968 7266 2579 Size: 3524 865 298 3291 Fragments arranged by size: 3524 3291 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6641 882 Size: 617 5142 2219 Fragments arranged by size: 5142 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3205 3223 4237 4318 4560 5275 5769 Size: 711 2001 18 1014 81 242 715 494 Cuts at: 5769 493 Size: 2702 Fragments arranged by size: 2702 2001 1014 715 711 494 242 81 18 BsaBI GATnn'nnATC Cuts at: 7972 7972 Size: 7978 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2196 2646 3348 3789 Size: 53 83 186 936 663 450 702 441 Cuts at: 3789 5147 5785 6167 6802 275 Size: 1358 638 382 635 1451 Fragments arranged by size: 1451 1358 936 702 663 638 635 450 441 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2165 3356 6411 6809 7083 7230 7641 2165 Size: 1191 3055 398 274 147 411 2502 Fragments arranged by size: 3055 2502 1191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 4100 4130 1178 Size: 30 165 30 1203 30 1464 30 5026 Fragments arranged by size: 5026 1464 1203 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2425 2614 2845 2917 2978 3754 3889 4078 Size: 1239 189 231 72 61 776 135 189 Cuts at: 4078 4655 4787 4958 1186 Size: 577 132 171 4206 Fragments arranged by size: 4206 1239 776 577 231 189 189 171 135 132 72 61 BseYI C'CCAG_C Cuts at: 2687 2777 2892 3057 3314 3747 3756 3852 4043 Size: 90 115 165 257 433 9 96 191 Cuts at: 4043 4434 7181 7677 2687 Size: 391 2747 496 2988 Fragments arranged by size: 2988 2747 496 433 391 257 191 165 115 96 90 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2068 3956 4298 4373 4775 5108 1163 Size: 573 332 1888 342 75 402 333 4033 Fragments arranged by size: 4033 1888 573 402 342 333 332 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2193 2306 3065 3086 3672 4231 Size: 275 186 192 113 759 21 586 559 Cuts at: 4231 5428 6189 7217 1540 Size: 1197 761 1028 2301 Fragments arranged by size: 2301 1197 1028 761 759 586 559 275 192 186 113 21 BsiHKAI G_wGCw'C Cuts at: 2865 3141 4260 4374 4443 5025 5232 6039 6124 Size: 276 1119 114 69 582 207 807 85 Cuts at: 6124 7195 2865 Size: 1071 3648 Fragments arranged by size: 3648 1119 1071 807 582 276 207 114 85 69 BsmI GAATG_Cn' Cuts at: 4849 5489 5582 4849 Size: 640 93 7245 Fragments arranged by size: 7245 640 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3934 4088 3934 Size: 154 7824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2448 2454 2688 2865 2970 3006 3113 Size: 399 658 6 234 177 105 36 107 Cuts at: 3113 3141 3239 3494 3560 3678 3884 3963 4006 Size: 28 98 255 66 118 206 79 43 Cuts at: 4006 4260 4374 4443 4881 4950 5025 5232 5297 Size: 254 114 69 438 69 75 207 65 Cuts at: 5297 6039 6124 7195 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 658 438 399 255 254 234 207 206 177 118 114 107 105 98 85 79 75 69 69 66 65 43 36 28 6 BspEI T'CCGG_A Cuts at: 3356 7641 3356 Size: 4285 3693 BspHI T'CATG_A Cuts at: 5867 7597 5867 Size: 1730 6248 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 3576 4849 5068 5333 844 Size: 1232 1500 1273 219 265 3489 Fragments arranged by size: 3489 1500 1273 1232 265 219 BsrBI CCG'CTC Cuts at: 1394 1823 2745 2956 3638 5865 1394 Size: 429 922 211 682 2227 3507 Fragments arranged by size: 3507 2227 922 682 429 211 BsrDI GCAATG_nn' Cuts at: 65 1248 2716 6473 6647 65 Size: 1183 1468 3757 174 1396 Fragments arranged by size: 3757 1468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 2188 2192 2301 3047 3191 3257 Size: 110 355 23 4 109 746 144 66 Cuts at: 3257 3814 4020 4230 4290 4389 4512 5092 6622 Size: 557 206 210 60 99 123 580 1530 Cuts at: 6622 6809 1700 Size: 187 2869 Fragments arranged by size: 2869 1530 746 580 557 355 210 206 187 144 123 110 109 99 66 60 23 4 BsrGI T'GTAC_A Cuts at: 96 4278 96 Size: 4182 3796 BssHII G'CGCG_C Cuts at: 1925 2371 3253 1925 Size: 446 882 6650 Fragments arranged by size: 6650 882 446 BssSI C'ACGA_G Cuts at: 4961 6038 7050 4961 Size: 1077 1012 5889 Fragments arranged by size: 5889 1077 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2476 3136 4360 5029 794 Size: 1682 660 1224 669 3743 Fragments arranged by size: 3743 1682 1224 669 660 BstBI TT'CG_AA Cuts at: 2007 6782 2007 Size: 4775 3203 BstEII G'GTnAC_C Cuts at: 1184 2161 4286 4793 5107 1184 Size: 977 2125 507 314 4055 Fragments arranged by size: 4055 2125 977 507 314 BstXI CCAn_nnnn'nTGG Cuts at: 2711 4180 4456 4888 5143 5257 2711 Size: 1469 276 432 255 114 5432 Fragments arranged by size: 5432 1469 432 276 255 114 BstYI r'GATC_y Cuts at: 1972 3909 4644 5308 5675 6060 6077 7529 7615 Size: 1937 735 664 367 385 17 1452 86 Cuts at: 7615 7627 7973 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1937 1452 735 664 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2499 3009 3263 3372 4429 5016 5136 Size: 552 1434 510 254 109 1057 587 120 Cuts at: 5136 513 Size: 3355 Fragments arranged by size: 3355 1434 1057 587 552 510 254 120 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4153 4966 487 Size: 683 56 2927 813 3499 Fragments arranged by size: 3499 2927 813 683 56 BtsI GCAGTG_nn' Cuts at: 3797 4310 4694 4982 5494 6288 6308 3797 Size: 513 384 288 512 794 20 5467 Fragments arranged by size: 5467 794 513 512 384 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7978 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7943 DraI TTT'AAA Cuts at: 5282 5282 Size: 7978 DraIII CAC_nnn'GTG Cuts at: 2295 2295 Size: 7978 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4901 5183 6985 809 Size: 507 3585 282 1802 1802 Fragments arranged by size: 3585 1802 1802 507 282 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2303 2331 Size: 54 1425 50 275 18 360 113 28 Cuts at: 2331 2599 3083 3588 3669 4332 4446 5013 5094 Size: 268 484 505 81 663 114 567 81 Cuts at: 5094 5133 5425 6314 7943 8 Size: 39 292 889 1629 43 Fragments arranged by size: 1629 1425 889 663 567 505 484 360 292 275 268 114 113 81 81 54 50 43 39 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2190 2303 3083 3669 5425 1537 Size: 275 378 113 780 586 1756 4090 Fragments arranged by size: 4090 1756 780 586 378 275 113 EarI CTCTTCn'nnn_ Cuts at: 1353 2542 2846 3500 3917 4154 4265 5908 1353 Size: 1189 304 654 417 237 111 1643 3423 Fragments arranged by size: 3423 1643 1189 654 417 304 237 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2396 4171 4400 6565 6939 7085 Size: 1064 138 807 1775 229 2165 374 146 Cuts at: 7085 387 Size: 1280 Fragments arranged by size: 2165 1775 1280 1064 807 374 229 146 138 EcoICRI GAG'CTC Cuts at: 2863 4258 4372 4441 2863 Size: 1395 114 69 6400 Fragments arranged by size: 6400 1395 114 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2462 2870 3077 3197 3223 3814 Size: 393 737 93 408 207 120 26 591 Cuts at: 3814 3892 3938 4033 4207 4346 4366 4436 4643 Size: 78 46 95 174 139 20 70 207 Cuts at: 4643 4798 4871 4940 4961 6041 6638 7425 7693 Size: 155 73 69 21 1080 597 787 268 Cuts at: 7693 1239 Size: 1524 Fragments arranged by size: 1524 1080 787 737 597 591 408 393 268 207 207 174 155 139 120 95 93 78 73 70 69 46 26 21 20 EcoNI CCTnn'n_nnAGG Cuts at: 2561 3590 3686 2561 Size: 1029 96 6853 Fragments arranged by size: 6853 1029 96 EcoO109I rG'GnC_Cy Cuts at: 2182 2619 2986 3109 3110 3380 3556 3557 3565 Size: 437 367 123 1 270 176 1 8 Cuts at: 3565 3988 4144 4357 4819 2182 Size: 423 156 213 462 5341 Fragments arranged by size: 5341 462 437 423 367 270 213 176 156 123 8 1 1 EcoRI G'AATT_C Cuts at: 5287 5665 5287 Size: 378 7600 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3916 3916 Size: 7978 FspI TGC'GCA Cuts at: 4079 6484 4079 Size: 2405 5573 HaeII r_GCGC'y Cuts at: 1678 2649 3166 3343 3543 3792 3848 4729 4741 Size: 971 517 177 200 249 56 881 12 Cuts at: 4741 4787 5150 7125 1678 Size: 46 363 1975 2531 Fragments arranged by size: 2531 1975 971 881 517 363 249 200 177 56 46 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3425 3452 3457 3484 6619 Size: 32 135 32 1853 27 5 27 3135 Cuts at: 6619 6651 6693 6725 1373 Size: 32 42 32 2626 Fragments arranged by size: 3135 2626 1853 135 42 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2065 5322 5568 669 Size: 988 281 127 3257 246 3079 Fragments arranged by size: 3257 3079 988 281 246 127 HindIII A'AGCT_T Cuts at: 748 748 Size: 7978 HpaI GTT'AAC Cuts at: 5568 5568 Size: 7978 KasI G'GCGC_C Cuts at: 2645 3788 5146 2645 Size: 1143 1358 5477 Fragments arranged by size: 5477 1358 1143 KpnI G_GTAC'C Cuts at: 4933 5303 4933 Size: 370 7608 MfeI C'AATT_G Cuts at: 5577 5577 Size: 7978 MluI A'CGCG_T Cuts at: 7854 7854 Size: 7978 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 5196 7092 7276 1305 Size: 780 3111 1896 184 2007 Fragments arranged by size: 3111 2007 1896 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 4334 4448 5135 10 Size: 54 1425 343 501 2001 114 687 2853 Fragments arranged by size: 2853 2001 1425 687 501 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 3014 4217 4712 5114 5141 5936 6295 Size: 648 1848 1203 495 402 27 795 359 Cuts at: 6295 6454 518 Size: 159 2042 Fragments arranged by size: 2042 1848 1203 795 648 495 402 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2329 2501 2529 2884 3061 3133 3214 3265 Size: 723 172 28 355 177 72 81 51 Cuts at: 3265 3415 3747 4035 4053 4384 4431 4744 5394 Size: 150 332 288 18 331 47 313 650 Cuts at: 5394 6071 7219 7464 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 723 677 650 355 332 331 313 288 245 177 172 150 81 72 51 47 28 18 NaeI GCC'GGC Cuts at: 2190 2194 3049 3193 3259 4022 4391 5094 2190 Size: 4 855 144 66 763 369 703 5074 Fragments arranged by size: 5074 855 763 703 369 144 66 4 NarI GG'CG_CC Cuts at: 2646 3789 5147 2646 Size: 1143 1358 5477 Fragments arranged by size: 5477 1358 1143 NcoI C'CATG_G Cuts at: 513 1065 3009 3372 5136 513 Size: 552 1944 363 1764 3355 Fragments arranged by size: 3355 1944 1764 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 7978 NgoMIV G'CCGG_C Cuts at: 2188 2192 3047 3191 3257 4020 4389 5092 2188 Size: 4 855 144 66 763 369 703 5074 Fragments arranged by size: 5074 855 763 703 369 144 66 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7978 NotI GC'GGCC_GC Cuts at: 5425 5425 Size: 7978 NspI r_CATG'y Cuts at: 1132 1229 2149 2662 4180 4655 4717 5336 6881 Size: 97 920 513 1518 475 62 619 1545 Cuts at: 6881 1132 Size: 2229 Fragments arranged by size: 2229 1545 1518 920 619 513 475 97 62 PasI CC'CwG_GG Cuts at: 1348 2704 2867 4749 4823 1348 Size: 1356 163 1882 74 4503 Fragments arranged by size: 4503 1882 1356 163 74 PciI A'CATG_T Cuts at: 6877 6877 Size: 7978 PflMI CCAn_nnn'nTGG Cuts at: 1108 3133 3343 3444 4344 5242 1108 Size: 2025 210 101 900 898 3844 Fragments arranged by size: 3844 2025 900 898 210 101 PmeI GTTT'AAAC Cuts at: 5282 5282 Size: 7978 PmlI CAC'GTG Cuts at: 4237 4318 4237 Size: 81 7897 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2940 2972 4100 4132 6047 6079 7560 7592 2940 Size: 32 1128 32 1915 32 1481 32 3326 Fragments arranged by size: 3326 1915 1481 1128 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 2619 3380 3565 3988 4819 2182 Size: 437 761 185 423 831 5341 Fragments arranged by size: 5341 831 761 437 423 185 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7978 PsiI TTA'TAA Cuts at: 5548 5548 Size: 7978 PspOMI G'GGCC_C Cuts at: 3109 3556 3109 Size: 447 7531 PstI C_TGCA'G Cuts at: 830 2343 2787 4701 4860 5079 5330 830 Size: 1513 444 1914 159 219 251 3478 Fragments arranged by size: 3478 1914 1513 444 251 219 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7978 PvuII CAG'CTG Cuts at: 3133 3214 3747 4053 4384 4744 3133 Size: 81 533 306 331 360 6367 Fragments arranged by size: 6367 533 360 331 306 81 SacI G_AGCT'C Cuts at: 2865 4260 4374 4443 2865 Size: 1395 114 69 6400 Fragments arranged by size: 6400 1395 114 69 SacII CC_GC'GG Cuts at: 2502 3266 4432 2502 Size: 764 1166 6048 Fragments arranged by size: 6048 1166 764 SalI G'TCGA_C Cuts at: 1936 2063 5320 1936 Size: 127 3257 4594 Fragments arranged by size: 4594 3257 127 SanDI GG'GwC_CC Cuts at: 2182 4819 2182 Size: 2637 5341 SapI GCTCTTCn'nnn_ Cuts at: 2542 3500 3917 4265 2542 Size: 958 417 348 6255 Fragments arranged by size: 6255 958 417 348 SbfI CC_TGCA'GG Cuts at: 5079 5330 5079 Size: 251 7727 ScaI AGT'ACT Cuts at: 4571 6226 7938 4571 Size: 1655 1712 4611 Fragments arranged by size: 4611 1712 1655 SexAI A'CCwGG_T Cuts at: 2157 2157 Size: 7978 SfcI C'TryA_G Cuts at: 826 1045 2339 2614 2783 3364 4586 4697 4856 Size: 219 1294 275 169 581 1222 111 159 Cuts at: 4856 5075 5326 6461 7142 7333 826 Size: 219 251 1135 681 191 1471 Fragments arranged by size: 1471 1294 1222 1135 681 581 275 251 219 219 191 169 159 111 SfoI GGC'GCC Cuts at: 2647 3790 5148 2647 Size: 1143 1358 5477 Fragments arranged by size: 5477 1358 1143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7978 SgrAI Cr'CCGG_yG Cuts at: 1700 2165 1700 Size: 465 7513 SmaI CCC'GGG Cuts at: 1872 2395 3489 3562 3811 5305 1872 Size: 523 1094 73 249 1494 4545 Fragments arranged by size: 4545 1494 1094 523 249 73 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 6082 6983 7245 820 Size: 197 926 615 3524 901 262 1553 Fragments arranged by size: 3524 1553 926 901 615 262 197 SnaBI TAC'GTA Cuts at: 493 5275 5769 493 Size: 4782 494 2702 Fragments arranged by size: 4782 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7978 SphI G_CATG'C Cuts at: 1132 2662 4655 4717 5336 1132 Size: 1530 1993 62 619 3774 Fragments arranged by size: 3774 1993 1530 619 62 SrfI GCCC'GGGC Cuts at: 3811 3811 Size: 7978 SspI AAT'ATT Cuts at: 5 52 5902 5 Size: 47 5850 2081 Fragments arranged by size: 5850 2081 47 StuI AGG'CCT Cuts at: 1517 4250 4887 1517 Size: 2733 637 4608 Fragments arranged by size: 4608 2733 637 StyI C'CwwG_G Cuts at: 513 1065 3009 3372 4299 4338 5136 5417 513 Size: 552 1944 363 927 39 798 281 3074 Fragments arranged by size: 3074 1944 927 798 552 363 281 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2101 2791 4274 4741 6005 6022 6175 Size: 1255 521 690 1483 467 1264 17 153 Cuts at: 6175 6360 6805 325 Size: 185 445 1498 Fragments arranged by size: 1498 1483 1264 1255 690 521 467 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3180 4278 4569 Size: 274 80 33 51 1443 1203 1098 291 Cuts at: 4569 6224 7936 96 Size: 1655 1712 138 Fragments arranged by size: 1712 1655 1443 1203 1098 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2030 2062 2724 2756 7938 7970 2030 Size: 32 662 32 5182 32 2038 Fragments arranged by size: 5182 2038 662 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4526 1316 Size: 3210 4768 XbaI T'CTAG_A Cuts at: 5314 5314 Size: 7978 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2437 2437 Size: 7978 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7978 XmaI C'CCGG_G Cuts at: 1870 2393 3487 3560 3809 5303 1870 Size: 523 1094 73 249 1494 4545 Fragments arranged by size: 4545 1494 1094 523 249 73 XmnI GAAnn'nnTTC Cuts at: 6107 6107 Size: 7978 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5786 276 Size: 53 83 186 936 4252 2468 Fragments arranged by size: 4252 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SfiI SwaI