(Circular) (Six-base) MAPSORT of: pFN21ASDA1686.seq Check: 5495 from: 1 to: 8251 pFN21ASDA1686 8251 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6061 278 Size: 53 83 186 936 4525 2468 Fragments arranged by size: 4525 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5594 1937 Size: 3657 4594 Acc65I G'GTAC_C Cuts at: 5572 5572 Size: 8251 AclI AA'CG_TT Cuts at: 1563 6378 6751 1563 Size: 4815 373 3063 Fragments arranged by size: 4815 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2376 2400 2721 3408 4107 4148 4689 4968 Size: 744 24 321 687 699 41 541 279 Cuts at: 4968 5155 5376 6314 7698 7966 1632 Size: 187 221 938 1384 268 1917 Fragments arranged by size: 1917 1384 938 744 699 687 541 321 279 268 221 187 41 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8054 AflIII A'CryG_T Cuts at: 1203 4307 7150 8127 1203 Size: 3104 2843 977 1327 Fragments arranged by size: 3104 2843 1327 977 AgeI A'CCGG_T Cuts at: 2527 7082 2527 Size: 4555 3696 AhdI GACnn_n'nnGTC Cuts at: 3805 6980 3805 Size: 3175 5076 AleI CACnn'nnGTG Cuts at: 1166 4425 1166 Size: 3259 4992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2210 1184 Size: 1026 7225 AlwNI CAG_nnn'CTG Cuts at: 2840 4685 5994 7566 2840 Size: 1845 1309 1572 3525 Fragments arranged by size: 3525 1845 1572 1309 ApaI G_GGCC'C Cuts at: 3210 3772 3210 Size: 562 7689 ApaLI G'TGCA_C Cuts at: 3113 4461 6308 7464 3113 Size: 1348 1847 1156 3900 Fragments arranged by size: 3900 1847 1348 1156 ApoI r'AATT_y Cuts at: 1493 2500 3061 3247 3993 4452 4488 5014 5242 Size: 1007 561 186 746 459 36 526 228 Cuts at: 5242 5560 5786 5938 8047 1493 Size: 318 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 746 561 526 459 318 228 226 186 152 36 AseI AT'TA_AT Cuts at: 160 1032 2712 3045 6805 160 Size: 872 1680 333 3760 1606 Fragments arranged by size: 3760 1680 1606 872 333 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8251 AvaI C'yCGr_G Cuts at: 1870 1943 4323 5576 1870 Size: 73 2380 1253 4545 Fragments arranged by size: 4545 2380 1253 73 BamHI G'GATC_C Cuts at: 5581 5948 5581 Size: 367 7884 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5572 7027 618 Size: 325 217 627 3785 1455 1842 Fragments arranged by size: 3785 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2444 3210 3367 3772 5570 1391 Size: 1053 766 157 405 1798 4072 Fragments arranged by size: 4072 1798 1053 766 405 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2092 3714 4933 928 Size: 971 193 1622 1219 4246 Fragments arranged by size: 4246 1622 1219 971 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6442 6476 1903 Size: 34 4505 34 3678 Fragments arranged by size: 4505 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2660 3680 3938 6143 7353 2660 Size: 1020 258 2205 1210 3558 Fragments arranged by size: 3558 2205 1210 1020 258 BclI T'GATC_A Cuts at: 1548 1548 Size: 8251 BfrBI ATG'CAT Cuts at: 4234 4234 Size: 8251 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 6862 136 Size: 107 122 71 631 605 776 4414 1525 Fragments arranged by size: 4414 1525 776 631 605 122 107 71 BglII A'GATC_T Cuts at: 3175 8246 3175 Size: 5071 3180 BlpI GC'TnA_GC Cuts at: 5668 5668 Size: 8251 Bme1580I G_kGCm'C Cuts at: 1790 3117 3210 3772 4465 6312 7468 1790 Size: 1327 93 562 693 1847 1156 2573 Fragments arranged by size: 2573 1847 1327 1156 693 562 93 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8251 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5982 6940 442 Size: 416 53 83 399 24 4565 958 1753 Fragments arranged by size: 4565 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8251 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 2068 Size: 8251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 6911 1239 Size: 824 4848 2579 Fragments arranged by size: 4848 2579 824 Bpu10I CC'TnA_GC Cuts at: 3780 5226 3780 Size: 1446 6805 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3154 3989 4214 4822 5295 6376 7241 7539 3154 Size: 835 225 608 473 1081 865 298 3866 Fragments arranged by size: 3866 1081 865 835 608 473 298 225 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6914 882 Size: 617 5415 2219 Fragments arranged by size: 5415 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4308 5548 6042 493 Size: 711 3104 1240 494 2702 Fragments arranged by size: 3104 2702 1240 711 494 BsaBI GATnn'nnATC Cuts at: 3278 8245 3278 Size: 4967 3284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6058 6440 7075 275 Size: 53 83 186 936 4525 382 635 1451 Fragments arranged by size: 4525 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2168 2527 6684 7082 7356 7503 7914 2168 Size: 359 4157 398 274 147 411 2505 Fragments arranged by size: 4157 2505 411 398 359 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3926 3956 3993 4023 1178 Size: 30 165 30 2523 30 37 30 5406 Fragments arranged by size: 5406 2523 165 37 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 3503 3753 3887 1186 Size: 943 1374 250 134 5550 Fragments arranged by size: 5550 1374 943 250 134 BseYI C'CCAG_C Cuts at: 2205 4246 7454 7950 2205 Size: 2041 3208 496 2506 Fragments arranged by size: 3208 2506 2041 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2752 4872 1163 Size: 573 387 629 2120 4542 Fragments arranged by size: 4542 2120 629 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2095 3229 5701 6462 7490 1540 Size: 275 186 94 1134 2472 761 1028 2301 Fragments arranged by size: 2472 2301 1134 1028 761 275 186 94 BsiHKAI G_wGCw'C Cuts at: 2129 2189 3117 3572 4465 6312 6397 7468 2129 Size: 60 928 455 893 1847 85 1071 2912 Fragments arranged by size: 2912 1847 1071 928 893 455 85 60 BsmI GAATG_Cn' Cuts at: 5762 5855 5762 Size: 93 8158 BsmBI CGTCTCn'nnnn_ Cuts at: 3895 3895 Size: 8251 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2189 2444 3117 3210 3367 3572 Size: 399 339 60 255 673 93 157 205 Cuts at: 3572 3772 4465 5570 6312 6397 7468 1391 Size: 200 693 1105 742 85 1071 2174 Fragments arranged by size: 2174 1105 1071 742 693 673 399 339 255 205 200 157 93 85 60 BspEI T'CCGG_A Cuts at: 7914 7914 Size: 8251 BspHI T'CATG_A Cuts at: 3298 3460 6140 7870 3298 Size: 162 2680 1730 3679 Fragments arranged by size: 3679 2680 1730 162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2279 5606 844 Size: 1435 3327 3489 Fragments arranged by size: 3489 3327 1435 BsrBI CCG'CTC Cuts at: 1394 1823 3485 6138 1394 Size: 429 1662 2653 3507 Fragments arranged by size: 3507 2653 1662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6746 6920 65 Size: 1183 5498 174 1396 Fragments arranged by size: 5498 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 2175 2527 6895 7082 1700 Size: 110 340 25 352 4368 187 2869 Fragments arranged by size: 4368 2869 352 340 187 110 25 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8251 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8251 BssSI C'ACGA_G Cuts at: 2460 6311 7323 2460 Size: 3851 1012 3388 Fragments arranged by size: 3851 3388 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3101 4204 794 Size: 2307 1103 4841 Fragments arranged by size: 4841 2307 1103 BstBI TT'CG_AA Cuts at: 2007 7055 2007 Size: 5048 3203 BstEII G'GTnAC_C Cuts at: 1184 2164 1184 Size: 980 7271 BstXI CCAn_nnnn'nTGG Cuts at: 3610 4030 3610 Size: 420 7831 BstYI r'GATC_y Cuts at: 1972 2032 2049 2931 3175 4825 5581 5948 6333 Size: 60 17 882 244 1650 756 367 385 Cuts at: 6333 6350 7802 7888 7900 8246 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1650 1452 882 756 385 367 346 244 86 60 17 17 12 Bsu36I CC'TnA_GG Cuts at: 4946 5289 5421 4946 Size: 343 132 7776 Fragments arranged by size: 7776 343 132 BtgI C'CryG_G Cuts at: 513 1065 2022 3609 3661 513 Size: 552 957 1587 52 5103 Fragments arranged by size: 5103 1587 957 552 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5472 487 Size: 683 56 4246 3266 Fragments arranged by size: 4246 3266 683 56 BtsI GCAGTG_nn' Cuts at: 2433 3448 4329 4860 5076 5457 5767 6561 6581 Size: 1015 881 531 216 381 310 794 20 Cuts at: 6581 2433 Size: 4103 Fragments arranged by size: 4103 1015 881 794 531 381 310 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8251 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8216 DraI TTT'AAA Cuts at: 5555 5555 Size: 8251 DraIII CAC_nnn'GTG Cuts at: 3522 3522 Size: 8251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3832 7258 809 Size: 507 2516 3426 1802 Fragments arranged by size: 3426 2516 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2372 5698 Size: 54 1425 50 275 18 262 280 3326 Cuts at: 5698 6587 8216 8 Size: 889 1629 43 Fragments arranged by size: 3326 1629 1425 889 280 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2092 5698 1537 Size: 275 280 3606 4090 Fragments arranged by size: 4090 3606 280 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2637 4149 4899 5340 6181 1353 Size: 1284 1512 750 441 841 3423 Fragments arranged by size: 3423 1512 1284 841 750 441 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 6838 7212 7358 387 Size: 1064 138 456 4793 374 146 1280 Fragments arranged by size: 4793 1280 1064 456 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2376 2400 2721 3408 4107 4148 Size: 393 431 313 24 321 687 699 41 Cuts at: 4148 4689 4968 5155 5376 6314 6911 7698 7966 Size: 541 279 187 221 938 597 787 268 Cuts at: 7966 1239 Size: 1524 Fragments arranged by size: 1524 938 787 699 687 597 541 431 393 321 313 279 268 221 187 41 24 EcoO109I rG'GnC_Cy Cuts at: 3206 3207 3476 3768 3206 Size: 1 269 292 7689 Fragments arranged by size: 7689 292 269 1 EcoRI G'AATT_C Cuts at: 3061 4452 4488 5560 5938 3061 Size: 1391 36 1072 378 5374 Fragments arranged by size: 5374 1391 1072 378 36 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4178 4178 Size: 8251 FspI TGC'GCA Cuts at: 6757 6757 Size: 8251 HaeII r_GCGC'y Cuts at: 1678 7398 1678 Size: 5720 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3074 3106 3791 3823 4810 Size: 32 135 32 1502 32 685 32 987 Cuts at: 4810 4842 5317 5349 6892 6924 6966 6998 1373 Size: 32 475 32 1543 32 42 32 2626 Fragments arranged by size: 2626 1543 1502 987 685 475 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4635 5380 5595 5841 669 Size: 988 281 2697 745 215 246 3079 Fragments arranged by size: 3079 2697 988 745 281 246 215 HindIII A'AGCT_T Cuts at: 748 2394 3965 4200 748 Size: 1646 1571 235 4799 Fragments arranged by size: 4799 1646 1571 235 HpaI GTT'AAC Cuts at: 4635 5841 4635 Size: 1206 7045 KpnI G_GTAC'C Cuts at: 5576 5576 Size: 8251 MfeI C'AATT_G Cuts at: 2308 5850 2308 Size: 3542 4709 MluI A'CGCG_T Cuts at: 8127 8127 Size: 8251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3243 4714 7365 7549 1305 Size: 1938 1471 2651 184 2007 Fragments arranged by size: 2651 2007 1938 1471 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6429 Fragments arranged by size: 6429 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3789 4425 5540 6209 6568 6727 518 Size: 648 2623 636 1115 669 359 159 2042 Fragments arranged by size: 2623 2042 1115 669 648 636 359 159 MspA1I CmG'CkG Cuts at: 1606 3818 4042 4414 5667 6344 7492 7737 1606 Size: 2212 224 372 1253 677 1148 245 2120 Fragments arranged by size: 2212 2120 1253 1148 677 372 245 224 NcoI C'CATG_G Cuts at: 513 1065 2022 3609 513 Size: 552 957 1587 5155 Fragments arranged by size: 5155 1587 957 552 NdeI CA'TA_TG Cuts at: 387 5206 387 Size: 4819 3432 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8251 NotI GC'GGCC_GC Cuts at: 5698 5698 Size: 8251 NsiI A_TGCA'T Cuts at: 4236 4236 Size: 8251 NspI r_CATG'y Cuts at: 1132 1229 2752 3099 3788 4148 4238 5609 7154 Size: 97 1523 347 689 360 90 1371 1545 Cuts at: 7154 1132 Size: 2229 Fragments arranged by size: 2229 1545 1523 1371 689 360 347 97 90 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8251 PciI A'CATG_T Cuts at: 7150 7150 Size: 8251 PflMI CCAn_nnn'nTGG Cuts at: 1108 3373 5164 1108 Size: 2265 1791 4195 Fragments arranged by size: 4195 2265 1791 PfoI T'CCnGG_A Cuts at: 2066 3930 2066 Size: 1864 6387 PmeI GTTT'AAAC Cuts at: 5555 5555 Size: 8251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6320 6352 7833 7865 6320 Size: 32 1481 32 6706 Fragments arranged by size: 6706 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3476 3476 Size: 8251 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8251 PsiI TTA'TAA Cuts at: 2553 3558 5821 2553 Size: 1005 2263 4983 Fragments arranged by size: 4983 2263 1005 PspOMI G'GGCC_C Cuts at: 3206 3768 3206 Size: 562 7689 PstI C_TGCA'G Cuts at: 830 3443 5603 830 Size: 2613 2160 3478 Fragments arranged by size: 3478 2613 2160 PvuI CG_AT'CG Cuts at: 2001 3229 2001 Size: 1228 7023 PvuII CAG'CTG Cuts at: 3818 4042 4414 3818 Size: 224 372 7655 Fragments arranged by size: 7655 372 224 SalI G'TCGA_C Cuts at: 1936 5593 1936 Size: 3657 4594 SapI GCTCTTCn'nnn_ Cuts at: 4899 5340 4899 Size: 441 7810 SbfI CC_TGCA'GG Cuts at: 5603 5603 Size: 8251 ScaI AGT'ACT Cuts at: 2566 3589 4882 6499 8211 2566 Size: 1023 1293 1617 1712 2606 Fragments arranged by size: 2606 1712 1617 1293 1023 SfcI C'TryA_G Cuts at: 826 1045 2445 3118 3439 4539 5599 6734 7415 Size: 219 1400 673 321 1100 1060 1135 681 Cuts at: 7415 7606 826 Size: 191 1471 Fragments arranged by size: 1471 1400 1135 1100 1060 681 673 321 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8251 SgrAI Cr'CCGG_yG Cuts at: 1700 2150 2175 1700 Size: 450 25 7776 Fragments arranged by size: 7776 450 25 SmaI CCC'GGG Cuts at: 1872 5578 1872 Size: 3706 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3133 3968 4193 4323 4801 5310 Size: 197 926 1190 835 225 130 478 509 Cuts at: 5310 6355 7256 7518 820 Size: 1045 901 262 1553 Fragments arranged by size: 1553 1190 1045 926 901 835 509 478 262 225 197 130 SnaBI TAC'GTA Cuts at: 493 5548 6042 493 Size: 5055 494 2702 Fragments arranged by size: 5055 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8251 SphI G_CATG'C Cuts at: 1132 3099 4148 4238 5609 1132 Size: 1967 1049 90 1371 3774 Fragments arranged by size: 3774 1967 1371 1049 90 SspI AAT'ATT Cuts at: 5 52 3619 6175 5 Size: 47 3567 2556 2081 Fragments arranged by size: 3567 2556 2081 47 StuI AGG'CCT Cuts at: 1517 3427 1517 Size: 1910 6341 StyI C'CwwG_G Cuts at: 513 1065 2022 3609 3856 4423 4889 5690 513 Size: 552 957 1587 247 567 466 801 3074 Fragments arranged by size: 3074 1587 957 801 567 552 466 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6278 6295 6448 6633 7078 325 Size: 1255 4698 17 153 185 445 1498 Fragments arranged by size: 4698 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2564 2827 3587 Size: 274 80 33 51 1443 587 263 760 Cuts at: 3587 4584 4880 6497 8209 96 Size: 997 296 1617 1712 138 Fragments arranged by size: 1712 1617 1443 997 760 587 296 274 263 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3993 4025 8211 8243 3993 Size: 32 4186 32 4001 Fragments arranged by size: 4186 4001 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8251 XbaI T'CTAG_A Cuts at: 5429 5587 5429 Size: 158 8093 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4261 4336 4730 4261 Size: 75 394 7782 Fragments arranged by size: 7782 394 75 XhoI C'TCGA_G Cuts at: 1943 4323 1943 Size: 2380 5871 XmaI C'CCGG_G Cuts at: 1870 5576 1870 Size: 3706 4545 XmnI GAAnn'nnTTC Cuts at: 3247 5133 6380 3247 Size: 1886 1247 5118 Fragments arranged by size: 5118 1886 1247 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6059 276 Size: 53 83 186 936 4525 2468 Fragments arranged by size: 4525 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI