Restriction map for
pFN21ASDA1674
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI MamI MroI NarI
NciI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1478 GTCGAC GT^[AC][GT]AC
AgeI 561 ACCGGT A^CCGGT
AlwNI 1048 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1499 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 591 CCCGAG C^[CT]CG[GA]G
BalI 1365 TGGCCA TGG^CCA
BbrPI 814 CACGTG CAC^GTG
BbsI 930 GAAGAC GAAGAC(2/6)
BfaI 71 CTAG C^TAG
BglII 1197 AGATCT A^GATCT
BlpI 1137 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1137 GCTCAGC GC^T[ACGT]AGC
BpuAI 930 GAAGAC GAAGAC(2/6)
BsaAI 814 CACGTG [CT]AC^GT[GA]
BsaI 617 GAGACC GGTCTC(1/5)
BsgI 469 GTGCAG GTGCAG(16/14)
BsmBI 1068 GAGACG CGTCTC(1/5)
BsmI 228 GAATGC GAATGC(1/-1)
BsoBI 591 CCCGAG C^[CT]CG[GA]G
BsrBI 1016 GAGCGG CCGCTC(-3/-3)
CelII 1137 GCTCAGC GC^T[ACGT]AGC
EaeI 1365 TGGCCA [CT]^GGCC[GA]
EcoNI 938 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 448 GAATTC G^AATTC
HgaI 625 GCGTC GACGC(5/10)
HincII 1478 GTCGAC GT[CT]^[GA]AC
HindII 1478 GTCGAC GT[CT]^[GA]AC
KspI 1156 CCGCGG CCGC^GG
MaeI 71 CTAG C^TAG
MfeI 211 CAATTG C^AATTG
MluI 623 ACGCGT A^CGCGT
MluNI 1365 TGGCCA TGG^CCA
MscI 1365 TGGCCA TGG^CCA
MslI 106 CACAAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1156 CCGCGG C[AC]G^C[GT]G
MunI 211 CAATTG C^AATTG
NaeI 1518 GCCGGC GCC^GGC
NcoI 1362 CCATGG C^CATGG
NgoMIV 1518 GCCGGC G^CCGGC
PinAI 561 ACCGGT A^CCGGT
PleI 742 GACTC GAGTC(4/5)
PmaCI 814 CACGTG CAC^GTG
PmlI 814 CACGTG CAC^GTG
PpuMI 653 AGGACCC [GA]G^G[AT]CC[CT]
SacII 1156 CCGCGG CCGC^GG
SalI 1478 GTCGAC G^TCGAC
SapI 1132 GAAGAGC GCTCTTC(1/4)
SbfI 1293 CCTGCAGG CCTGCA^GG
Sse8387I 1293 CCTGCAGG CCTGCA^GG
TaqI 1479 TCGA T^CGA
TthHB8I 1479 TCGA T^CGA
XmnI 1499 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 624 CGCG CG^CG
1157 CGCG
AcsI 448 GAATTC [GA]^AATT[CT]
1305 AAATTT
AflIII 623 ACGCGT A^C[GA][CT]GT
915 ACACGT
AhdI 619 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
961 GACCTCTTGTC
AlwI 987 GATCC GGATC(4/5)
1404 GGATC
Aor51HI 312 AGCGCT AGC^GCT
494 AGCGCT
ApoI 448 GAATTC [GA]^AATT[CT]
1305 AAATTT
AspEI 619 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
961 GACCTCTTGTC
AvaII 288 GGACC G^G[AT]CC
654 GGACC
BbvCI 457 CCTCAGC CCTCAGC(-5/-2)
792 GCTGAGG
BsaWI 293 TCCGGT [AT]^CCGG[AT]
561 ACCGGT
BsmAI 617 GAGAC GTCTC(1/5)
1068 GAGAC
BsrFI 561 ACCGGT [GA]^CCGG[CT]
1518 GCCGGC
BstUI 624 CGCG CG^CG
1157 CGCG
BstYI 1197 AGATCT [GA]^GATC[CT]
1404 GGATCT
Cfr10I 561 ACCGGT [GA]^CCGG[CT]
1518 GCCGGC
DraII 653 AGGACCC [GA]G^G[ACGT]CC[CT]
1370 AGGCCCC
DsaI 1156 CCGCGG C^C[GA][CT]GG
1362 CCATGG
Eam1105I 619 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
961 GACCTCTTGTC
Eco47III 312 AGCGCT AGC^GCT
494 AGCGCT
EcoO109I 653 AGGACCC [GA]G^G[ACGT]CC[CT]
1370 AGGCCCC
EcoT14I 633 CCAAGG C^C[AT][AT]GG
1362 CCATGG
MflI 1197 AGATCT [GA]^GATC[CT]
1404 GGATCT
MvnI 624 CGCG CG^CG
1157 CGCG
NlaIII 1167 CATG CATG^
1363 CATG
PstI 1087 CTGCAG CTGCA^G
1294 CTGCAG
StyI 633 CCAAGG C^C[AT][AT]GG
1362 CCATGG
Tsp45I 584 GTGAC ^GT[GC]AC
969 GTCAC
XhoII 1197 AGATCT [GA]^GATC[CT]
1404 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIV RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp509I
TspRI