(Circular) (Six-base) MAPSORT of: pFN21ASDA1674.seq Check: 4132 from: 1 to: 6319 pFN21ASDA1674 6319 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3502 3662 1937 Size: 1565 160 4594 Fragments arranged by size: 4594 1565 160 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4382 5766 6034 1632 Size: 2750 1384 268 1917 Fragments arranged by size: 2750 1917 1384 268 AfeI AGC'GCT Cuts at: 2337 2519 2337 Size: 182 6137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 2646 2938 5218 6195 1203 Size: 1443 292 2280 977 1327 Fragments arranged by size: 2280 1443 1327 977 292 AgeI A'CCGG_T Cuts at: 2584 5150 2584 Size: 2566 3753 AhdI GACnn_n'nnGTC Cuts at: 2647 2989 5048 2647 Size: 342 2059 3918 Fragments arranged by size: 3918 2059 342 AleI CACnn'nnGTG Cuts at: 1166 2133 1166 Size: 967 5352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2268 3188 1184 Size: 1084 920 4315 Fragments arranged by size: 4315 1084 920 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2535 2567 2535 Size: 32 6287 AlwNI CAG_nnn'CTG Cuts at: 3076 4062 5634 3076 Size: 986 1572 3761 Fragments arranged by size: 3761 1572 986 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2471 3328 3628 3854 4006 6115 1493 Size: 978 857 300 226 152 2109 1697 Fragments arranged by size: 2109 1697 978 857 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2614 3644 1870 Size: 73 671 1030 4545 Fragments arranged by size: 4545 1030 671 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3640 5095 618 Size: 325 217 627 1853 1455 1842 Fragments arranged by size: 1853 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2359 2621 3060 3133 3162 3638 1391 Size: 968 262 439 73 29 476 4072 Fragments arranged by size: 4072 968 476 439 262 73 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2960 928 Size: 971 1061 4287 Fragments arranged by size: 4287 1061 971 BbvCI CC'TCA_GC Cuts at: 2481 2816 2481 Size: 335 5984 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3220 6314 3220 Size: 3094 3225 BlpI GC'TnA_GC Cuts at: 3161 3736 3161 Size: 575 5744 Bme1580I G_kGCm'C Cuts at: 1790 3109 3316 4380 5536 1790 Size: 1319 207 1064 1156 2573 Fragments arranged by size: 2573 1319 1156 1064 207 BmgBI CAC'GTC Cuts at: 1318 3355 1318 Size: 2037 4282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4050 5008 442 Size: 416 53 83 399 24 2633 958 1753 Fragments arranged by size: 2633 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2882 2882 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2648 2658 2950 3044 3422 4979 1239 Size: 854 555 10 292 94 378 1557 2579 Fragments arranged by size: 2579 1557 854 555 378 292 94 10 Bpu10I CC'TnA_GC Cuts at: 2481 2673 2816 2481 Size: 192 143 5984 Fragments arranged by size: 5984 192 143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2197 2256 2556 2879 4444 5309 5607 2197 Size: 59 300 323 1565 865 298 2909 Fragments arranged by size: 2909 1565 865 323 300 298 59 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2634 4982 882 Size: 617 1135 2348 2219 Fragments arranged by size: 2348 2219 1135 617 BsaAI yAC'GTr Cuts at: 493 1204 2839 3616 4110 493 Size: 711 1635 777 494 2702 Fragments arranged by size: 2702 1635 777 711 494 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 2584 4752 5150 5424 5571 5982 2316 Size: 268 2168 398 274 147 411 2653 Fragments arranged by size: 2653 2168 411 398 274 268 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2535 2565 1178 Size: 30 165 30 1132 30 4932 Fragments arranged by size: 4932 1132 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2149 2219 2469 2758 2804 2807 2953 Size: 904 59 70 250 289 46 3 146 Cuts at: 2953 3199 3419 1186 Size: 246 220 4086 Fragments arranged by size: 4086 904 289 250 246 220 146 70 59 46 3 BseYI C'CCAG_C Cuts at: 2222 2419 2783 5522 6018 2222 Size: 197 364 2739 496 2523 Fragments arranged by size: 2739 2523 496 364 197 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2513 1163 Size: 573 777 4969 Fragments arranged by size: 4969 777 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2359 2838 3060 3162 4380 4465 5536 2359 Size: 479 222 102 1218 85 1071 3142 Fragments arranged by size: 3142 1218 1071 479 222 102 85 BsmI GAATG_Cn' Cuts at: 2257 3830 3923 2257 Size: 1573 93 4653 Fragments arranged by size: 4653 1573 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3085 3085 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2621 2838 3060 3109 3133 3162 Size: 399 569 262 217 222 49 24 29 Cuts at: 3162 3316 3638 4380 4465 5536 1391 Size: 154 322 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 399 322 262 222 217 154 85 49 29 24 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3674 844 Size: 2830 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3041 4206 1394 Size: 429 1218 1165 3507 Fragments arranged by size: 3507 1218 1165 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2584 3541 4963 5150 1700 Size: 110 774 957 1422 187 2869 Fragments arranged by size: 2869 1422 957 774 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 2007 5123 2007 Size: 3116 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstYI r'GATC_y Cuts at: 1972 3220 3427 3649 4016 4401 4418 5870 5956 Size: 1248 207 222 367 385 17 1452 86 Cuts at: 5956 5968 6314 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 222 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3179 3385 513 Size: 552 957 1157 206 3447 Fragments arranged by size: 3447 1157 957 552 206 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2458 3107 3835 4629 4649 2458 Size: 649 728 794 20 4128 Fragments arranged by size: 4128 794 728 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3388 3766 4655 Size: 54 1425 50 275 18 1558 378 889 Cuts at: 4655 6284 8 Size: 1629 43 Fragments arranged by size: 1629 1558 1425 889 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3024 3140 3150 3330 4249 1353 Size: 1671 116 10 180 919 3423 Fragments arranged by size: 3423 1671 919 180 116 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3243 4906 5280 5426 387 Size: 1064 138 1654 1663 374 146 1280 Fragments arranged by size: 1663 1654 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2357 3058 3160 2357 Size: 701 102 5516 Fragments arranged by size: 5516 701 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2648 2658 2950 3044 3422 4382 Size: 393 461 555 10 292 94 378 960 Cuts at: 4382 4979 5766 6034 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 960 787 597 555 461 393 378 292 268 94 10 EcoNI CCTnn'n_nnAGG Cuts at: 2965 2965 Size: 6319 EcoO109I rG'GnC_Cy Cuts at: 2677 3394 2677 Size: 717 5602 EcoRI G'AATT_C Cuts at: 2471 3628 4006 2471 Size: 1157 378 4784 Fragments arranged by size: 4784 1157 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2728 2920 2728 Size: 192 6127 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2339 2521 2806 3050 5466 1678 Size: 661 182 285 244 2416 2531 Fragments arranged by size: 2531 2416 661 285 244 182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2133 2165 2341 2373 2633 Size: 32 135 32 561 32 176 32 260 Cuts at: 2633 2665 2975 3007 4960 4992 5034 5066 1373 Size: 32 310 32 1953 32 42 32 2626 Fragments arranged by size: 2626 1953 561 310 260 176 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3503 3663 3909 669 Size: 988 281 1565 160 246 3079 Fragments arranged by size: 3079 1565 988 281 246 160 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 2234 3918 2234 Size: 1684 4635 MluI A'CGCG_T Cuts at: 2646 6195 2646 Size: 3549 2770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2119 2588 5433 5617 1305 Size: 814 469 2845 184 2007 Fragments arranged by size: 2845 2007 814 469 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3390 10 Size: 54 1425 343 1558 2939 Fragments arranged by size: 2939 1558 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2133 4277 4636 4795 518 Size: 648 967 2144 359 159 2042 Fragments arranged by size: 2144 2042 967 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3181 3735 4412 5560 5805 1606 Size: 1575 554 677 1148 245 2120 Fragments arranged by size: 2120 1575 1148 677 554 245 NaeI GCC'GGC Cuts at: 3543 3543 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2022 3385 513 Size: 552 957 1363 3447 Fragments arranged by size: 3447 1363 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 3541 3541 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3677 5222 1132 Size: 97 2448 1545 2229 Fragments arranged by size: 2448 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6319 PfoI T'CCnGG_A Cuts at: 2966 3060 2966 Size: 94 6225 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 2839 2839 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2535 2567 4388 4420 5901 5933 2535 Size: 32 1821 32 1481 32 2921 Fragments arranged by size: 2921 1821 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2677 2677 Size: 6319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PstI C_TGCA'G Cuts at: 830 3114 3321 3671 830 Size: 2284 207 350 3478 Fragments arranged by size: 3478 2284 350 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SacI G_AGCT'C Cuts at: 2359 3060 3162 2359 Size: 701 102 5516 Fragments arranged by size: 5516 701 102 SacII CC_GC'GG Cuts at: 3182 3182 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3501 3661 1936 Size: 1565 160 4594 Fragments arranged by size: 4594 1565 160 SapI GCTCTTCn'nnn_ Cuts at: 3150 3150 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3321 3671 3321 Size: 350 5969 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 3110 3317 3449 3667 4802 5483 5674 Size: 219 2065 207 132 218 1135 681 191 Cuts at: 5674 826 Size: 1471 Fragments arranged by size: 2065 1471 1135 681 219 218 207 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2176 2271 2571 2894 4423 5324 Size: 197 926 233 95 300 323 1529 901 Cuts at: 5324 5586 820 Size: 262 1553 Fragments arranged by size: 1553 1529 926 901 323 300 262 233 197 95 SnaBI TAC'GTA Cuts at: 493 3616 4110 493 Size: 3123 494 2702 Fragments arranged by size: 3123 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2022 2656 3385 3758 513 Size: 552 957 634 729 373 3074 Fragments arranged by size: 3074 957 729 634 552 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4346 4363 4516 4701 5146 325 Size: 1255 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4565 6277 96 Size: 274 80 33 51 1443 2588 1712 138 Fragments arranged by size: 2588 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 3526 4448 3526 Size: 922 5397 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI