Restriction map for
pFN21ASDA1674
(Linear) (Six-base) MAPSORT of: KIAA1674.seq Check: 7204 from: 1 to: 1590
KIAA1674.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 1479 1590
Size: 1479 111
AfeI AGC'GCT
Cuts at: 0 314 496 1590
Size: 314 182 1094
Fragments arranged by size:
1094 314 182
AflIII A'CryG_T
Cuts at: 0 623 915 1590
Size: 623 292 675
Fragments arranged by size:
675 623 292
AgeI A'CCGG_T
Cuts at: 0 561 1590
Size: 561 1029
AhdI GACnn_n'nnGTC
Cuts at: 0 624 966 1590
Size: 624 342 624
Fragments arranged by size:
624 624 342
AleI CACnn'nnGTG
Cuts at: 0 110 1590
Size: 110 1480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 245 1165 1590
Size: 245 920 425
Fragments arranged by size:
920 425 245
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 544 1590
Size: 512 32 1046
Fragments arranged by size:
1046 512 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1053 1590
Size: 1053 537
ApoI r'AATT_y
Cuts at: 0 448 1305 1590
Size: 448 857 285
Fragments arranged by size:
857 448 285
AvaI C'yCGr_G
Cuts at: 0 591 1590
Size: 591 999
BanII G_rGCy'C
Cuts at: 0 336 598 1037 1110 1139 1590
Size: 336 262 439 73 29 451
Fragments arranged by size:
451 439 336 262 73 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 937 1590
Size: 937 653
BbvCI CC'TCA_GC
Cuts at: 0 458 793 1590
Size: 458 335 797
Fragments arranged by size:
797 458 335
BglII A'GATC_T
Cuts at: 0 1197 1590
Size: 1197 393
BlpI GC'TnA_GC
Cuts at: 0 1138 1590
Size: 1138 452
Bme1580I G_kGCm'C
Cuts at: 0 1086 1293 1590
Size: 1086 207 297
Fragments arranged by size:
1086 297 207
BmgBI CAC'GTC
Cuts at: 0 1332 1590
Size: 1332 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 859 1590
Size: 859 731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 625 635 927 1021 1399 1590
Size: 70 555 10 292 94 378 191
Fragments arranged by size:
555 378 292 191 94 70 10
Bpu10I CC'TnA_GC
Cuts at: 0 458 650 793 1590
Size: 458 192 143 797
Fragments arranged by size:
797 458 192 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 233 533 856 1590
Size: 174 59 300 323 734
Fragments arranged by size:
734 323 300 174 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 611 1590
Size: 611 979
BsaAI yAC'GTr
Cuts at: 0 816 1590
Size: 816 774
BsaWI w'CCGG_w
Cuts at: 0 293 561 1590
Size: 293 268 1029
Fragments arranged by size:
1029 293 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 512 542 1590
Size: 512 30 1048
Fragments arranged by size:
1048 512 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 126 196 446 735 781 784 930
Size: 67 59 70 250 289 46 3 146
Cuts at: 930 1176 1396 1590
Size: 246 220 194
Fragments arranged by size:
289 250 246 220 194 146 70 67
59 46 3
BseYI C'CCAG_C
Cuts at: 0 199 396 760 1590
Size: 199 197 364 830
Fragments arranged by size:
830 364 199 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1590
Size: 490 1100
BsiHKAI G_wGCw'C
Cuts at: 0 336 815 1037 1139 1590
Size: 336 479 222 102 451
Fragments arranged by size:
479 451 336 222 102
BsmI GAATG_Cn'
Cuts at: 0 234 1590
Size: 234 1356
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1062 1590
Size: 1062 528
Bsp1286I G_dGCh'C
Cuts at: 0 336 598 815 1037 1086 1110 1139 1293
Size: 336 262 217 222 49 24 29 154
Cuts at: 1293 1590
Size: 297
Fragments arranged by size:
336 297 262 222 217 154 49 29
24
BsrBI CCG'CTC
Cuts at: 0 1018 1590
Size: 1018 572
BsrFI r'CCGG_y
Cuts at: 0 561 1518 1590
Size: 561 957 72
Fragments arranged by size:
957 561 72
BstYI r'GATC_y
Cuts at: 0 1197 1404 1590
Size: 1197 207 186
Fragments arranged by size:
1197 207 186
BtgI C'CryG_G
Cuts at: 0 1156 1362 1590
Size: 1156 206 228
Fragments arranged by size:
1156 228 206
BtsI GCAGTG_nn'
Cuts at: 0 435 1084 1590
Size: 435 649 506
Fragments arranged by size:
649 506 435
EaeI y'GGCC_r
Cuts at: 0 1365 1590
Size: 1365 225
EarI CTCTTCn'nnn_
Cuts at: 0 1001 1117 1127 1307 1590
Size: 1001 116 10 180 283
Fragments arranged by size:
1001 283 180 116 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1220 1590
Size: 1220 370
EcoICRI GAG'CTC
Cuts at: 0 334 1035 1137 1590
Size: 334 701 102 453
Fragments arranged by size:
701 453 334 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 625 635 927 1021 1399 1590
Size: 70 555 10 292 94 378 191
Fragments arranged by size:
555 378 292 191 94 70 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 942 1590
Size: 942 648
EcoO109I rG'GnC_Cy
Cuts at: 0 654 1371 1590
Size: 654 717 219
Fragments arranged by size:
717 654 219
EcoRI G'AATT_C
Cuts at: 0 448 1590
Size: 448 1142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 705 897 1590
Size: 705 192 693
Fragments arranged by size:
705 693 192
HaeII r_GCGC'y
Cuts at: 0 316 498 783 1027 1590
Size: 316 182 285 244 563
Fragments arranged by size:
563 316 285 244 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 318 350 610 642 952 984
Size: 110 32 176 32 260 32 310 32
Cuts at: 984 1590
Size: 606
Fragments arranged by size:
606 310 260 176 110 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1480 1590
Size: 1480 110
MfeI C'AATT_G
Cuts at: 0 211 1590
Size: 211 1379
MluI A'CGCG_T
Cuts at: 0 623 1590
Size: 623 967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 565 1590
Size: 96 469 1025
Fragments arranged by size:
1025 469 96
MscI TGG'CCA
Cuts at: 0 1367 1590
Size: 1367 223
MslI CAynn'nnrTG
Cuts at: 0 110 1590
Size: 110 1480
MspA1I CmG'CkG
Cuts at: 0 1158 1590
Size: 1158 432
NaeI GCC'GGC
Cuts at: 0 1520 1590
Size: 1520 70
NcoI C'CATG_G
Cuts at: 0 1362 1590
Size: 1362 228
NgoMIV G'CCGG_C
Cuts at: 0 1518 1590
Size: 1518 72
PfoI T'CCnGG_A
Cuts at: 0 943 1037 1590
Size: 943 94 553
Fragments arranged by size:
943 553 94
PmlI CAC'GTG
Cuts at: 0 816 1590
Size: 816 774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 512 544 1590
Size: 512 32 1046
Fragments arranged by size:
1046 512 32
PpuMI rG'GwC_Cy
Cuts at: 0 654 1590
Size: 654 936
PstI C_TGCA'G
Cuts at: 0 1091 1298 1590
Size: 1091 207 292
Fragments arranged by size:
1091 292 207
SacI G_AGCT'C
Cuts at: 0 336 1037 1139 1590
Size: 336 701 102 451
Fragments arranged by size:
701 451 336 102
SacII CC_GC'GG
Cuts at: 0 1159 1590
Size: 1159 431
SalI G'TCGA_C
Cuts at: 0 1478 1590
Size: 1478 112
SapI GCTCTTCn'nnn_
Cuts at: 0 1127 1590
Size: 1127 463
SbfI CC_TGCA'GG
Cuts at: 0 1298 1590
Size: 1298 292
SfcI C'TryA_G
Cuts at: 0 1087 1294 1426 1590
Size: 1087 207 132 164
Fragments arranged by size:
1087 207 164 132
SmlI C'TyrA_G
Cuts at: 0 153 248 548 871 1590
Size: 153 95 300 323 719
Fragments arranged by size:
719 323 300 153 95
StyI C'CwwG_G
Cuts at: 0 633 1362 1590
Size: 633 729 228
Fragments arranged by size:
729 633 228
XmnI GAAnn'nnTTC
Cuts at: 0 1503 1590
Size: 1503 87
Enzymes that do cut:
AccI AfeI AflIII AgeI AhdI AleI AlfI AloI
AlwNI ApoI AvaI BanII BbsI BbvCI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BsrBI BsrFI BstYI BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV PfoI PmlI PpiI PpuMI PstI
SacI SacII SalI SapI SbfI SfcI SmlI StyI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BcgI BciVI BclI BfrBI BglI BmrI BmtI BsaBI
BsaHI BsiEI BsiWI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI