Restriction map for
pFN21ASDA1663
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanII BbrPI BcgI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KspI MaeII MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 131 GGTACC G^GTACC
AcsI 553 AAATTT [GA]^AATT[CT]
AlwI 283 GATCC GGATC(4/5)
AocI 259 CCTGAGG CC^T[ACGT]AGG
ApoI 553 AAATTT [GA]^AATT[CT]
Asp718I 131 GGTACC G^GTACC
AvaII 192 GGACC G^G[AT]CC
BbeI 65 GGCGCC GGCGC^C
BbsI 945 GAAGAC GAAGAC(2/6)
BbvCI 966 CCTCAGC CCTCAGC(-5/-2)
BciVI 993 GTATCC GTATCC(6/5)
BglI 733 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 511 GCTCAGC GC^T[ACGT]AGC
BpmI 940 CTGGAG CTGGAG(16/14)
Bpu1102I 511 GCTCAGC GC^T[ACGT]AGC
BpuAI 945 GAAGAC GAAGAC(2/6)
BsiEI 821 CGGCCG CG[GA][CT]^CG
BsmFI 750 GTCCC GGGAC(10/14)
BsmI 711 GCATTC GAATGC(1/-1)
BspMI 877 ACCTGC ACCTGC(4/8)
BstXI 906 CCAGGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 259 CCTGAGG CC^T[ACGT]AGG
CelII 511 GCTCAGC GC^T[ACGT]AGC
CfoI 66 GCGC GCG^C
EagI 821 CGGCCG C^GGCCG
EclXI 821 CGGCCG C^GGCCG
Eco52I 821 CGGCCG C^GGCCG
Eco81I 259 CCTGAGG CC^T[ACGT]AGG
EcoT14I 770 CCAAGG C^C[AT][AT]GG
EcoT22I 709 ATGCAT ATGCA^T
HaeII 65 GGCGCC [GA]GCGC^[CT]
HgaI 406 GACGC GACGC(5/10)
HhaI 66 GCGC GCG^C
HinP1I 66 GCGC G^CGC
KasI 65 GGCGCC G^GCGCC
KpnI 131 GGTACC GGTAC^C
MaeIII 480 GTCAC ^GT[ACGT]AC
MslI 564 CACTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 65 GGCGCC GG^CGCC
NsiI 709 ATGCAT ATGCA^T
SfaNI 817 GATGC GCATC(5/9)
SfiI 732 GGCCAACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 65 GGCGCC GGC^GCC
StuI 963 AGGCCT AGG^CCT
StyI 770 CCAAGG C^C[AT][AT]GG
Tsp45I 480 GTCAC ^GT[GC]AC
Tsp509I 554 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 65 GGCGCC G[GA]^CG[CT]C
406 GACGCC
BfaI 7 CTAG C^TAG
94 CTAG
BmyI 362 GTGCCC G[AGT]GC[ACT]^C
421 GTGCCC
BsaHI 65 GGCGCC G[GA]^CG[CT]C
406 GACGCC
BseRI 88 GAGGAG GAGGAG(10/8)
262 GAGGAG
Bsp1286I 362 GTGCCC G[AGT]GC[ACT]^C
421 GTGCCC
Bsp1407I 47 TGTACA T^GTACA
782 TGTACA
BsrGI 47 TGTACA T^GTACA
782 TGTACA
BsrI 128 ACTGG ACTGG(1/-1)
477 CCAGT
BstF5I 467 CATCC GGATG(2/0)
342 GGATG
DsaI 197 CCGTGG C^C[GA][CT]GG
1016 CCGTGG
EarI 808 CTCTTC CTCTTC(1/4)
934 CTCTTC
FokI 467 CATCC GGATG(9/13)
342 GGATG
Hin1I 65 GGCGCC G[GA]^CG[CT]C
406 GACGCC
Ksp632I 808 CTCTTC CTCTTC(1/4)
934 CTCTTC
MaeI 7 CTAG C^TAG
94 CTAG
MspA1I 165 CCGCTG C[AC]G^C[GT]G
837 CAGCGG
PflMI 466 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1011 CCAGCCCGTGG
SspBI 47 TGTACA T^GTACA
782 TGTACA
Van91I 466 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1011 CCAGCCCGTGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI BalI BanI
BbvI BcnI BsaJI BsiYI BslI
BsrBI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI HaeIII HapII HpaII HphI
ItaI MboI MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI TspRI