Restriction map for
pFN21ASDA1663
(Circular) (Six-base) MAPSORT of: pFN21ASDA1663.seq Check: 7163 from: 1 to: 5761
pFN21ASDA1663 5761 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2909 2909
Size: 5761
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2154 3082 2154
Size: 928 4833
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2726 3824 5208 5476 1632
Size: 1094 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1098 1094 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 3504 5076 3504
Size: 1572 4189
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2576 3070 3296 3448 5557 1493
Size: 1083 494 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 494 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2154 2384 2864 2897
Size: 325 217 627 301 66 230 480 33
Cuts at: 2897 2945 3082 4537 618
Size: 48 137 1455 1842
Fragments arranged by size:
1842 1455 627 480 325 301 230 217
137 66 48 33
BanII G_rGCy'C
Cuts at: 1391 3080 1391
Size: 1689 4072
BbeI G_GCGC'C
Cuts at: 2092 2092
Size: 5761
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2975 928
Size: 971 1076 3714
Fragments arranged by size:
3714 1076 971
BbvCI CC'TCA_GC
Cuts at: 2990 2990
Size: 5761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3027 3653 4863 3027
Size: 626 1210 3925
Fragments arranged by size:
3925 1210 626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BfrBI ATG'CAT
Cuts at: 2734 2734
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2762 4372 136
Size: 107 122 71 631 605 1090 1610 1525
Fragments arranged by size:
1610 1525 1090 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2535 3178 2535
Size: 643 5118
Bme1580I G_kGCm'C
Cuts at: 1790 2389 2448 3822 4978 1790
Size: 599 59 1374 1156 2573
Fragments arranged by size:
2573 1374 1156 599 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2984 4421 1239
Size: 1745 1437 2579
Fragments arranged by size:
2579 1745 1437
Bpu10I CC'TnA_GC
Cuts at: 2990 2990
Size: 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3058 3552 493
Size: 711 1854 494 2702
Fragments arranged by size:
2702 1854 711 494
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 2430 3568 3950
Size: 53 83 186 936 556 341 1138 382
Cuts at: 3950 4585 275
Size: 635 1451
Fragments arranged by size:
1451 1138 936 635 556 382 341 186
83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2789 2819 1178
Size: 30 165 30 1386 30 4120
Fragments arranged by size:
4120 1386 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2300 1186
Size: 940 174 4647
Fragments arranged by size:
4647 940 174
BseYI C'CCAG_C
Cuts at: 2507 2775 2825 3020 4964 5460 2507
Size: 268 50 195 1944 496 2808
Fragments arranged by size:
2808 1944 496 268 195 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2847 3211 3972 5000 1540
Size: 275 186 846 364 761 1028 2301
Fragments arranged by size:
2301 1028 846 761 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsmI GAATG_Cn'
Cuts at: 2734 3272 3365 2734
Size: 538 93 5130
Fragments arranged by size:
5130 538 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2389 2448 3080 3822 3907 4978 1391
Size: 399 599 59 632 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 632 599 399 85 59
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 3116 844
Size: 2065 207 3489
Fragments arranged by size:
3489 2065 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 2529 2534 2685 3648 1394
Size: 429 299 407 5 151 963 3507
Fragments arranged by size:
3507 963 429 407 299 151 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 2070 2805 96
Size: 1974 735 3052
Fragments arranged by size:
3052 1974 735
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 2007 4565 2007
Size: 2558 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 2936 2936
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756
Size: 1119 367 385 17 1452 86 12 346
Cuts at: 5756 1972
Size: 1977
Fragments arranged by size:
1977 1452 1119 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2283 2283
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2220 3039 513
Size: 552 1155 819 3235
Fragments arranged by size:
3235 1155 819 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2469 487
Size: 683 56 1243 3779
Fragments arranged by size:
3779 1243 683 56
BtsI GCAGTG_nn'
Cuts at: 2644 2893 3277 4071 4091 2644
Size: 249 384 794 20 4314
Fragments arranged by size:
4314 794 384 249 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2227 2262 2560 2595 2797 2832 529
Size: 35 1663 35 298 35 202 35 3458
Fragments arranged by size:
3458 1663 298 202 35 35 35 35
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2497 2650
Size: 54 1425 50 275 18 402 265 153
Cuts at: 2650 2754 2844 2851 3048 3208 4097 5726 8
Size: 104 90 7 197 160 889 1629 43
Fragments arranged by size:
1629 1425 889 402 275 265 197 160
153 104 90 54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2844 3208 1537
Size: 275 1032 364 4090
Fragments arranged by size:
4090 1032 364 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 2963 3691 1353
Size: 1484 126 728 3423
Fragments arranged by size:
3423 1484 728 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2726 2984 3824 4421 5208 5476 1239
Size: 393 1094 258 840 597 787 268 1524
Fragments arranged by size:
1524 1094 840 787 597 393 268 258
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2992 2434
Size: 558 5203
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2092 4908 1678
Size: 414 2816 2531
Fragments arranged by size:
2816 2531 414
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373
Size: 32 135 32 2830 32 42 32 2626
Fragments arranged by size:
2830 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KasI G'GCGC_C
Cuts at: 2088 2088
Size: 5761
KpnI G_GTAC'C
Cuts at: 2158 3086 2158
Size: 928 4833
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2499 4875 5059 1305
Size: 1194 2376 184 2007
Fragments arranged by size:
2376 2007 1194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2499 2652 2853 3050 10
Size: 54 1425 343 667 153 201 197 2721
Fragments arranged by size:
2721 1425 667 343 201 197 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 3719 4078 4237 518
Size: 648 1425 1128 359 159 2042
Fragments arranged by size:
2042 1425 1128 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2190 2862 3177 3854 5002 5247 1606
Size: 584 672 315 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 584 315 245
NarI GG'CG_CC
Cuts at: 2089 2089
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5209
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NsiI A_TGCA'T
Cuts at: 2736 2736
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2495 3040 1108
Size: 1387 545 3829
Fragments arranged by size:
3829 1387 545
PfoI T'CCnGG_A
Cuts at: 2961 2961
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 3113 830
Size: 2283 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 3109 4244 4925 5116 826
Size: 219 2064 1135 681 191 1471
Fragments arranged by size:
2064 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2762 2762
Size: 5761
SfoI GGC'GCC
Cuts at: 2090 2090
Size: 5761
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3058 3552 493
Size: 2565 494 2702
Fragments arranged by size:
2702 2565 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 2988 1517
Size: 1471 4290
StyI C'CwwG_G
Cuts at: 513 1065 2793 3200 513
Size: 552 1728 407 3074
Fragments arranged by size:
3074 1728 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2521 2704 2800 2839 3788 3805 3958
Size: 1255 941 183 96 39 949 17 153
Cuts at: 3958 4143 4588 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 949 941 445 185 183 153
96 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2070 2805 4007
Size: 274 80 33 51 1443 93 735 1202
Cuts at: 4007 5719 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1202 735 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SrfI SwaI XcmI