Restriction map for
pFN21ASDA1663
(Linear) (Six-base) MAPSORT of: KIAA1663.seq Check: 7882 from: 1 to: 1032
KIAA1663.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 1032
Size: 886 146
Acc65I G'GTAC_C
Cuts at: 0 131 1032
Size: 131 901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1032
Size: 703 329
ApoI r'AATT_y
Cuts at: 0 553 1032
Size: 553 479
BanI G'GyrC_C
Cuts at: 0 65 131 361 841 874 922 1032
Size: 65 66 230 480 33 48 110
Fragments arranged by size:
480 230 110 66 65 48 33
BbeI G_GCGC'C
Cuts at: 0 69 1032
Size: 69 963
BbsI GAAGACnn'nnnn_
Cuts at: 0 952 1032
Size: 952 80
BbvCI CC'TCA_GC
Cuts at: 0 967 1032
Size: 967 65
BciVI GTATCCnnnnn_n'
Cuts at: 0 1004 1032
Size: 1004 28
BfrBI ATG'CAT
Cuts at: 0 711 1032
Size: 711 321
BglI GCCn_nnn'nGGC
Cuts at: 0 739 1032
Size: 739 293
BlpI GC'TnA_GC
Cuts at: 0 512 1032
Size: 512 520
Bme1580I G_kGCm'C
Cuts at: 0 366 425 1032
Size: 366 59 607
Fragments arranged by size:
607 366 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 716 1032
Size: 716 316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1032
Size: 961 71
Bpu10I CC'TnA_GC
Cuts at: 0 967 1032
Size: 967 65
BsaHI Gr'CG_yC
Cuts at: 0 66 407 1032
Size: 66 341 625
Fragments arranged by size:
625 341 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1032
Size: 766 30 236
Fragments arranged by size:
766 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 277 1032
Size: 103 174 755
Fragments arranged by size:
755 174 103
BseYI C'CCAG_C
Cuts at: 0 484 752 802 997 1032
Size: 484 268 50 195 35
Fragments arranged by size:
484 268 195 50 35
BsiEI CG_ry'CG
Cuts at: 0 824 1032
Size: 824 208
BsmI GAATG_Cn'
Cuts at: 0 711 1032
Size: 711 321
Bsp1286I G_dGCh'C
Cuts at: 0 366 425 1032
Size: 366 59 607
Fragments arranged by size:
607 366 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 1032
Size: 886 146
BsrBI CCG'CTC
Cuts at: 0 99 506 511 662 1032
Size: 99 407 5 151 370
Fragments arranged by size:
407 370 151 99 5
BsrGI T'GTAC_A
Cuts at: 0 47 782 1032
Size: 47 735 250
Fragments arranged by size:
735 250 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 913 1032
Size: 913 119
Bsu36I CC'TnA_GG
Cuts at: 0 260 1032
Size: 260 772
BtgI C'CryG_G
Cuts at: 0 197 1016 1032
Size: 197 819 16
Fragments arranged by size:
819 197 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 446 1032
Size: 446 586
BtsI GCAGTG_nn'
Cuts at: 0 621 870 1032
Size: 621 249 162
Fragments arranged by size:
621 249 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 204 239 537 572 774 809 1032
Size: 204 35 298 35 202 35 223
Fragments arranged by size:
298 223 204 202 35 35 35
EaeI y'GGCC_r
Cuts at: 0 209 474 627 731 821 828 1025 1032
Size: 209 265 153 104 90 7 197 7
Fragments arranged by size:
265 209 197 153 104 90 7 7
EagI C'GGCC_G
Cuts at: 0 821 1032
Size: 821 211
EarI CTCTTCn'nnn_
Cuts at: 0 814 940 1032
Size: 814 126 92
Fragments arranged by size:
814 126 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 961 1032
Size: 703 258 71
Fragments arranged by size:
703 258 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 411 969 1032
Size: 411 558 63
Fragments arranged by size:
558 411 63
HaeII r_GCGC'y
Cuts at: 0 69 1032
Size: 69 963
KasI G'GCGC_C
Cuts at: 0 65 1032
Size: 65 967
KpnI G_GTAC'C
Cuts at: 0 135 1032
Size: 135 897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1032
Size: 476 556
MscI TGG'CCA
Cuts at: 0 476 629 830 1027 1032
Size: 476 153 201 197 5
Fragments arranged by size:
476 201 197 153 5
MslI CAynn'nnrTG
Cuts at: 0 568 1032
Size: 568 464
MspA1I CmG'CkG
Cuts at: 0 167 839 1032
Size: 167 672 193
Fragments arranged by size:
672 193 167
NarI GG'CG_CC
Cuts at: 0 66 1032
Size: 66 966
NsiI A_TGCA'T
Cuts at: 0 713 1032
Size: 713 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 472 1017 1032
Size: 472 545 15
Fragments arranged by size:
545 472 15
PfoI T'CCnGG_A
Cuts at: 0 938 1032
Size: 938 94
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 739 1032
Size: 739 293
SfoI GGC'GCC
Cuts at: 0 67 1032
Size: 67 965
StuI AGG'CCT
Cuts at: 0 965 1032
Size: 965 67
StyI C'CwwG_G
Cuts at: 0 770 1032
Size: 770 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 498 681 777 816 1032
Size: 498 183 96 39 216
Fragments arranged by size:
498 216 183 96 39
TatI w'GTAC_w
Cuts at: 0 47 782 1032
Size: 47 735 250
Fragments arranged by size:
735 250 47
Enzymes that do cut:
AarI Acc65I AcuI ApoI BanI BbeI BbsI BbvCI
BciVI BfrBI BglI BlpI Bme1580I BplI BpmI Bpu10I
BsaHI BsaXI BseRI BseYI BsiEI BsmI Bsp1286I BspMI
BsrBI BsrGI BstXI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EagI EarI Eco57MI FalI HaeII KasI KpnI
MmeI MscI MslI MspA1I NarI NsiI PflMI PfoI
SfiI SfoI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BcgI BclI
BglII BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI
BsaWI BsgI BsiHKAI BsiWI BsmBI BspEI BspHI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
ClaI DraI DraIII DrdI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI