(Circular) (Six-base) MAPSORT of: pFN21ASDA1627.seq Check: 2511 from: 1 to: 6097 pFN21ASDA1627 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2394 3440 1937 Size: 457 1046 4594 Fragments arranged by size: 4594 1046 457 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2342 820 Size: 197 1325 4575 Fragments arranged by size: 4575 1325 197 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 3372 1166 Size: 2206 3891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2296 2416 2576 3009 3406 3632 3784 5893 Size: 803 120 160 433 397 226 152 2109 Cuts at: 5893 1493 Size: 1697 Fragments arranged by size: 2109 1697 803 433 397 226 160 152 120 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 3422 1870 Size: 73 1479 4545 Fragments arranged by size: 4545 1479 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2201 2234 2201 Size: 33 6064 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3418 4873 618 Size: 325 217 627 330 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 330 325 217 BanII G_rGCy'C Cuts at: 1391 2478 3259 3416 1391 Size: 1087 781 157 4072 Fragments arranged by size: 4072 1087 781 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 2840 3052 928 Size: 971 351 590 212 3973 Fragments arranged by size: 3973 971 590 351 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BfrBI ATG'CAT Cuts at: 3146 3146 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3314 6092 3314 Size: 2778 3319 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 4158 5314 1790 Size: 2368 1156 2573 Fragments arranged by size: 2573 2368 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4757 1239 Size: 3518 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3394 3888 493 Size: 711 2190 494 2702 Fragments arranged by size: 2702 2190 711 494 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3049 3904 4286 4921 Size: 53 83 186 936 1516 855 382 635 Cuts at: 4921 275 Size: 1451 Fragments arranged by size: 1516 1451 936 855 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2182 2212 1178 Size: 30 165 30 779 30 5063 Fragments arranged by size: 5063 779 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2508 3213 3261 3342 3375 1186 Size: 880 442 705 48 81 33 3908 Fragments arranged by size: 3908 880 705 442 81 48 33 BseYI C'CCAG_C Cuts at: 5300 5796 5300 Size: 496 5601 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 3163 3259 3367 4158 4243 5314 2478 Size: 685 96 108 791 85 1071 3261 Fragments arranged by size: 3261 1071 791 685 108 96 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2478 3163 3259 3367 3416 4158 4243 Size: 399 688 685 96 108 49 742 85 Cuts at: 4243 5314 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 688 685 399 108 96 85 49 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2053 3984 1394 Size: 429 230 1931 3507 Fragments arranged by size: 3507 1931 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3185 96 Size: 3089 3008 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 2007 4901 2007 Size: 2894 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2043 2848 3314 3427 3794 4179 4196 5648 Size: 71 805 466 113 367 385 17 1452 Cuts at: 5648 5734 5746 6092 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 805 466 385 367 346 113 86 71 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3295 3355 513 Size: 552 957 1273 60 3255 Fragments arranged by size: 3255 1273 957 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2634 2881 3613 4407 4427 2634 Size: 247 732 794 20 4304 Fragments arranged by size: 4304 794 732 247 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3292 3544 4433 Size: 54 1425 50 275 18 1462 252 889 Cuts at: 4433 6062 8 Size: 1629 43 Fragments arranged by size: 1629 1462 1425 889 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2307 2749 3061 3268 4027 1353 Size: 954 442 312 207 759 3423 Fragments arranged by size: 3423 954 759 442 312 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 3257 2476 Size: 781 5316 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4160 4757 5544 5812 1239 Size: 393 2528 597 787 268 1524 Fragments arranged by size: 2528 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2187 2878 2187 Size: 691 5406 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 2347 2379 2833 Size: 32 135 32 489 32 254 32 454 Cuts at: 2833 2865 4738 4770 4812 4844 1373 Size: 32 1873 32 42 32 2626 Fragments arranged by size: 2626 1873 489 454 254 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2950 3441 3687 669 Size: 988 281 1012 491 246 3079 Fragments arranged by size: 3079 1012 988 491 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2724 2783 3082 5211 5395 1305 Size: 1419 59 299 2129 184 2007 Fragments arranged by size: 2129 2007 1419 299 184 59 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2943 3372 4055 4414 4573 518 Size: 648 1777 429 683 359 159 2042 Fragments arranged by size: 2042 1777 683 648 429 359 159 MspA1I CmG'CkG Cuts at: 1606 3513 4190 5338 5583 1606 Size: 1907 677 1148 245 2120 Fragments arranged by size: 2120 1907 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4588 Fragments arranged by size: 4588 957 552 NdeI CA'TA_TG Cuts at: 387 2305 3142 387 Size: 1918 837 3342 Fragments arranged by size: 3342 1918 837 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NsiI A_TGCA'T Cuts at: 3148 3148 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 3455 5000 1132 Size: 97 2226 1545 2229 Fragments arranged by size: 2229 2226 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2871 2903 4166 4198 5679 5711 2871 Size: 32 1263 32 1481 32 3257 Fragments arranged by size: 3257 1481 1263 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 SacI G_AGCT'C Cuts at: 2478 3259 2478 Size: 781 5316 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 2335 4345 6057 2335 Size: 2010 1712 2375 Fragments arranged by size: 2375 2010 1712 SfcI C'TryA_G Cuts at: 826 1045 3003 3445 4580 5261 5452 826 Size: 219 1958 442 1135 681 191 1471 Fragments arranged by size: 1958 1471 1135 681 442 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 4201 5102 5364 820 Size: 197 926 399 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 399 262 197 SnaBI TAC'GTA Cuts at: 493 3394 3888 493 Size: 2901 494 2702 Fragments arranged by size: 2901 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 2615 4021 5 Size: 47 2563 1406 2081 Fragments arranged by size: 2563 2081 1406 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2022 2749 3536 513 Size: 552 957 727 787 3074 Fragments arranged by size: 3074 957 787 727 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2587 3258 4124 4141 4294 4479 4924 Size: 1255 1007 671 866 17 153 185 445 Cuts at: 4924 325 Size: 1498 Fragments arranged by size: 1498 1255 1007 866 671 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2333 2526 3074 Size: 274 80 33 51 1443 356 193 548 Cuts at: 3074 3185 4343 6055 96 Size: 111 1158 1712 138 Fragments arranged by size: 1712 1443 1158 548 356 274 193 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 3012 4226 3012 Size: 1214 4883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI