(Circular) (Six-base) MAPSORT of: pFN21ASDA1617.seq Check: 6870 from: 1 to: 7411 pFN21ASDA1617 7411 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5221 278 Size: 53 83 186 936 3685 2468 Fragments arranged by size: 3685 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4754 1937 Size: 2817 4594 Acc65I G'GTAC_C Cuts at: 4732 4732 Size: 7411 AclI AA'CG_TT Cuts at: 1563 5538 5911 1563 Size: 3975 373 3063 Fragments arranged by size: 3975 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2588 2775 3842 4649 5474 6858 7126 Size: 510 446 187 1067 807 825 1384 268 Cuts at: 7126 1632 Size: 1917 Fragments arranged by size: 1917 1384 1067 825 807 510 446 268 187 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7214 AflIII A'CryG_T Cuts at: 1203 2215 2289 6310 7287 1203 Size: 1012 74 4021 977 1327 Fragments arranged by size: 4021 1327 1012 977 74 AgeI A'CCGG_T Cuts at: 6242 6242 Size: 7411 AhdI GACnn_n'nnGTC Cuts at: 4264 6140 4264 Size: 1876 5535 AleI CACnn'nnGTG Cuts at: 1166 4505 1166 Size: 3339 4072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3719 3913 1184 Size: 2535 194 4682 Fragments arranged by size: 4682 2535 194 AlwNI CAG_nnn'CTG Cuts at: 2332 3527 5154 6726 2332 Size: 1195 1627 1572 3017 Fragments arranged by size: 3017 1627 1572 1195 ApaLI G'TGCA_C Cuts at: 2422 5468 6624 2422 Size: 3046 1156 3209 Fragments arranged by size: 3209 3046 1156 ApoI r'AATT_y Cuts at: 1493 2489 2864 3802 3892 3934 4720 4946 5098 Size: 996 375 938 90 42 786 226 152 Cuts at: 5098 7207 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 996 938 786 375 226 152 90 42 AseI AT'TA_AT Cuts at: 160 1032 5965 160 Size: 872 4933 1606 Fragments arranged by size: 4933 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 AvaI C'yCGr_G Cuts at: 1870 1943 4165 4424 4736 1870 Size: 73 2222 259 312 4545 Fragments arranged by size: 4545 2222 312 259 73 BamHI G'GATC_C Cuts at: 4741 5108 4741 Size: 367 7044 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3105 4276 4372 4732 6187 Size: 325 217 627 1318 1171 96 360 1455 Cuts at: 6187 618 Size: 1842 Fragments arranged by size: 1842 1455 1318 1171 627 360 325 217 96 BanII G_rGCy'C Cuts at: 1391 2964 4166 4242 4329 4730 1391 Size: 1573 1202 76 87 401 4072 Fragments arranged by size: 4072 1573 1202 401 87 76 BbeI G_GCGC'C Cuts at: 4376 4376 Size: 7411 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4131 928 Size: 971 2232 4208 Fragments arranged by size: 4208 2232 971 BbvCI CC'TCA_GC Cuts at: 3609 4387 3609 Size: 778 6633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5602 5636 1903 Size: 34 3665 34 3678 Fragments arranged by size: 3678 3665 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5303 6513 5303 Size: 1210 6201 BclI T'GATC_A Cuts at: 1548 1548 Size: 7411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4544 6022 136 Size: 107 122 71 631 605 2872 1478 1525 Fragments arranged by size: 2872 1525 1478 631 605 122 107 71 BglII A'GATC_T Cuts at: 4032 7406 4032 Size: 3374 4037 BlpI GC'TnA_GC Cuts at: 2092 4828 2092 Size: 2736 4675 Bme1580I G_kGCm'C Cuts at: 1790 2426 4281 4412 4540 5472 6628 1790 Size: 636 1855 131 128 932 1156 2573 Fragments arranged by size: 2573 1855 1156 932 636 131 128 BmgBI CAC'GTC Cuts at: 1318 4507 1318 Size: 3189 4222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2167 2305 3175 Size: 416 53 83 399 24 750 138 870 Cuts at: 3175 3404 4687 5142 6100 442 Size: 229 1283 455 958 1753 Fragments arranged by size: 1753 1283 958 870 750 455 416 399 229 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7411 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3143 3997 4249 4297 3143 Size: 854 252 48 6257 Fragments arranged by size: 6257 854 252 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4493 6071 1239 Size: 3254 1578 2579 Fragments arranged by size: 3254 2579 1578 Bpu10I CC'TnA_GC Cuts at: 3609 4387 3609 Size: 778 6633 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 2960 5536 6401 6699 2116 Size: 844 2576 865 298 2828 Fragments arranged by size: 2828 2576 865 844 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2103 4220 6074 882 Size: 617 604 2117 1854 2219 Fragments arranged by size: 2219 2117 1854 617 604 BsaAI yAC'GTr Cuts at: 493 1204 2292 2319 2501 4708 5202 493 Size: 711 1088 27 182 2207 494 2702 Fragments arranged by size: 2702 2207 1088 711 494 182 27 BsaBI GATnn'nnATC Cuts at: 7405 7405 Size: 7411 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4197 4373 5218 5600 Size: 53 83 186 936 2664 176 845 382 Cuts at: 5600 6235 275 Size: 635 1451 Fragments arranged by size: 2664 1451 936 845 635 382 186 176 83 53 BsaWI w'CCGG_w Cuts at: 5844 6242 6516 6663 7074 5844 Size: 398 274 147 411 6181 Fragments arranged by size: 6181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7186 Fragments arranged by size: 7186 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3123 4199 4238 4375 4466 4469 4472 1186 Size: 1937 1076 39 137 91 3 3 4125 Fragments arranged by size: 4125 1937 1076 137 91 39 3 3 BseYI C'CCAG_C Cuts at: 4633 6614 7110 4633 Size: 1981 496 4934 Fragments arranged by size: 4934 1981 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3432 4142 4631 1163 Size: 573 1696 710 489 3943 Fragments arranged by size: 3943 1696 710 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4861 5622 6650 1540 Size: 275 186 2860 761 1028 2301 Fragments arranged by size: 2860 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2033 2426 4242 5472 5557 6628 2033 Size: 393 1816 1230 85 1071 2816 Fragments arranged by size: 2816 1816 1230 1071 393 85 BsiWI C'GTAC_G Cuts at: 3868 3868 Size: 7411 BsmI GAATG_Cn' Cuts at: 2229 4922 5015 2229 Size: 2693 93 4625 Fragments arranged by size: 4625 2693 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3809 3809 Size: 7411 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2426 2964 4166 4242 4281 4329 Size: 399 243 393 538 1202 76 39 48 Cuts at: 4329 4412 4540 4730 5472 5557 6628 1391 Size: 83 128 190 742 85 1071 2174 Fragments arranged by size: 2174 1202 1071 742 538 399 393 243 190 128 85 83 76 48 39 BspEI T'CCGG_A Cuts at: 7074 7074 Size: 7411 BspHI T'CATG_A Cuts at: 3544 5300 7030 3544 Size: 1756 1730 3925 Fragments arranged by size: 3925 1756 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4766 844 Size: 3922 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4317 5298 1394 Size: 429 2494 981 3507 Fragments arranged by size: 3507 2494 981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5906 6080 65 Size: 1183 4658 174 1396 Fragments arranged by size: 4658 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4229 4335 4375 6055 6242 1700 Size: 110 2419 106 40 1680 187 2869 Fragments arranged by size: 2869 2419 1680 187 110 106 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7411 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7411 BssSI C'ACGA_G Cuts at: 5471 6483 5471 Size: 1012 6399 BstAPI GCAn_nnn'nTGC Cuts at: 794 2192 2332 794 Size: 1398 140 5873 Fragments arranged by size: 5873 1398 140 BstBI TT'CG_AA Cuts at: 2007 6215 2007 Size: 4208 3203 BstEII G'GTnAC_C Cuts at: 1184 4498 1184 Size: 3314 4097 BstXI CCAn_nnnn'nTGG Cuts at: 2539 2754 3102 3417 2539 Size: 215 348 315 6533 Fragments arranged by size: 6533 348 315 215 BstYI r'GATC_y Cuts at: 1972 3790 4032 4741 5108 5493 5510 6962 7048 Size: 1818 242 709 367 385 17 1452 86 Cuts at: 7048 7060 7406 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1818 1452 709 385 367 346 242 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2412 3396 4146 4158 4200 4366 Size: 552 957 390 984 750 12 42 166 Cuts at: 4366 513 Size: 3558 Fragments arranged by size: 3558 984 957 750 552 390 166 42 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2380 487 Size: 683 56 1154 5518 Fragments arranged by size: 5518 1154 683 56 BtsI GCAGTG_nn' Cuts at: 4927 5721 5741 4927 Size: 794 20 6597 Fragments arranged by size: 6597 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7411 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7376 DraI TTT'AAA Cuts at: 4715 4715 Size: 7411 DraIII CAC_nnn'GTG Cuts at: 2413 2413 Size: 7411 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6418 809 Size: 507 5102 1802 Fragments arranged by size: 5102 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 3151 4337 Size: 54 1425 50 275 18 471 850 1186 Cuts at: 4337 4545 4576 4858 5747 7376 8 Size: 208 31 282 889 1629 43 Fragments arranged by size: 1629 1425 1186 889 850 471 282 275 208 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 4858 1537 Size: 275 3046 4090 Fragments arranged by size: 4090 3046 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 2877 3470 3620 3807 4176 5341 1353 Size: 816 708 593 150 187 369 1165 3423 Fragments arranged by size: 3423 1165 816 708 593 369 187 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4108 4286 5998 6372 6518 387 Size: 1064 138 2519 178 1712 374 146 1280 Fragments arranged by size: 2519 1712 1280 1064 374 178 146 138 EcoICRI GAG'CTC Cuts at: 4240 4240 Size: 7411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2588 2775 3842 4493 4649 5474 Size: 393 510 446 187 1067 651 156 825 Cuts at: 5474 6071 6858 7126 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1067 825 787 651 597 510 446 393 268 187 156 EcoNI CCTnn'n_nnAGG Cuts at: 2553 3138 2553 Size: 585 6826 EcoO109I rG'GnC_Cy Cuts at: 2546 2564 3641 4637 2546 Size: 18 1077 996 5320 Fragments arranged by size: 5320 1077 996 18 EcoRI G'AATT_C Cuts at: 4720 5098 4720 Size: 378 7033 EcoRV GAT'ATC Cuts at: 3957 3957 Size: 7411 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3740 3740 Size: 7411 FspI TGC'GCA Cuts at: 5917 5917 Size: 7411 HaeII r_GCGC'y Cuts at: 1678 4376 6558 1678 Size: 2698 2182 2531 Fragments arranged by size: 2698 2531 2182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2687 2719 3377 Size: 32 135 32 476 32 607 32 658 Cuts at: 3377 3409 4250 4282 6052 6084 6126 6158 1373 Size: 32 841 32 1770 32 42 32 2626 Fragments arranged by size: 2626 1770 841 658 607 476 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3589 4755 5001 669 Size: 988 281 1651 1166 246 3079 Fragments arranged by size: 3079 1651 1166 988 281 246 HindIII A'AGCT_T Cuts at: 748 2134 2936 748 Size: 1386 802 5223 Fragments arranged by size: 5223 1386 802 HpaI GTT'AAC Cuts at: 5001 5001 Size: 7411 KasI G'GCGC_C Cuts at: 4372 4372 Size: 7411 KpnI G_GTAC'C Cuts at: 4736 4736 Size: 7411 MfeI C'AATT_G Cuts at: 3509 5010 3509 Size: 1501 5910 MluI A'CGCG_T Cuts at: 7287 7287 Size: 7411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2638 4467 4628 6525 6709 1305 Size: 1333 1829 161 1897 184 2007 Fragments arranged by size: 2007 1897 1829 1333 184 161 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 3153 4547 10 Size: 54 1425 343 471 850 1394 2874 Fragments arranged by size: 2874 1425 1394 850 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4118 4505 5369 5728 5887 518 Size: 648 2952 387 864 359 159 2042 Fragments arranged by size: 2952 2042 864 648 387 359 159 MspA1I CmG'CkG Cuts at: 1606 2329 3781 4071 4160 4368 4827 5504 6652 Size: 723 1452 290 89 208 459 677 1148 Cuts at: 6652 6897 1606 Size: 245 2120 Fragments arranged by size: 2120 1452 1148 723 677 459 290 245 208 89 NaeI GCC'GGC Cuts at: 4337 4377 4337 Size: 40 7371 NarI GG'CG_CC Cuts at: 4373 4373 Size: 7411 NcoI C'CATG_G Cuts at: 513 1065 2022 3396 4200 513 Size: 552 957 1374 804 3724 Fragments arranged by size: 3724 1374 957 804 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7411 NgoMIV G'CCGG_C Cuts at: 4335 4375 4335 Size: 40 7371 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7411 NotI GC'GGCC_GC Cuts at: 4858 4858 Size: 7411 NspI r_CATG'y Cuts at: 1132 1229 4769 6314 1132 Size: 97 3540 1545 2229 Fragments arranged by size: 3540 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7411 PciI A'CATG_T Cuts at: 6310 6310 Size: 7411 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7411 PfoI T'CCnGG_A Cuts at: 2538 3286 3807 4242 2538 Size: 748 521 435 5707 Fragments arranged by size: 5707 748 521 435 PmeI GTTT'AAAC Cuts at: 4715 4715 Size: 7411 PmlI CAC'GTG Cuts at: 2319 2319 Size: 7411 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5480 5512 6993 7025 5480 Size: 32 1481 32 5866 Fragments arranged by size: 5866 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2546 2546 Size: 7411 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7411 PsiI TTA'TAA Cuts at: 4981 4981 Size: 7411 PstI C_TGCA'G Cuts at: 830 3360 4763 830 Size: 2530 1403 3478 Fragments arranged by size: 3478 2530 1403 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7411 PvuII CAG'CTG Cuts at: 2329 3781 2329 Size: 1452 5959 SacI G_AGCT'C Cuts at: 4242 4242 Size: 7411 SacII CC_GC'GG Cuts at: 4161 4369 4161 Size: 208 7203 SalI G'TCGA_C Cuts at: 1936 4753 1936 Size: 2817 4594 SapI GCTCTTCn'nnn_ Cuts at: 3620 3620 Size: 7411 SbfI CC_TGCA'GG Cuts at: 4763 4763 Size: 7411 ScaI AGT'ACT Cuts at: 2630 5659 7371 2630 Size: 3029 1712 2670 Fragments arranged by size: 3029 2670 1712 SfcI C'TryA_G Cuts at: 826 1045 2568 3356 4759 5894 6575 6766 826 Size: 219 1523 788 1403 1135 681 191 1471 Fragments arranged by size: 1523 1471 1403 1135 788 681 219 191 SfoI GGC'GCC Cuts at: 4374 4374 Size: 7411 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7411 SmaI CCC'GGG Cuts at: 1872 4738 1872 Size: 2866 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2131 2939 5515 6416 6678 820 Size: 197 926 188 808 2576 901 262 1553 Fragments arranged by size: 2576 1553 926 901 808 262 197 188 SnaBI TAC'GTA Cuts at: 493 4708 5202 493 Size: 4215 494 2702 Fragments arranged by size: 4215 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7411 SphI G_CATG'C Cuts at: 1132 4769 1132 Size: 3637 3774 SspI AAT'ATT Cuts at: 5 52 2168 5335 5 Size: 47 2116 3167 2081 Fragments arranged by size: 3167 2116 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7411 StyI C'CwwG_G Cuts at: 513 1065 2022 2073 3396 4200 4542 4850 513 Size: 552 957 51 1323 804 342 308 3074 Fragments arranged by size: 3074 1323 957 804 552 342 308 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 5438 5455 5608 5793 6238 325 Size: 1255 707 3151 17 153 185 445 1498 Fragments arranged by size: 3151 1498 1255 707 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2628 2831 Size: 274 80 33 51 1443 495 156 203 Cuts at: 2831 3097 5657 7369 96 Size: 266 2560 1712 138 Fragments arranged by size: 2560 1712 1443 495 274 266 203 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2626 2658 7371 7403 2626 Size: 32 4713 32 2634 Fragments arranged by size: 4713 2634 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2390 3884 1316 Size: 1074 1494 4843 Fragments arranged by size: 4843 1494 1074 XbaI T'CTAG_A Cuts at: 4747 4747 Size: 7411 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7411 XmaI C'CCGG_G Cuts at: 1870 4736 1870 Size: 2866 4545 XmnI GAAnn'nnTTC Cuts at: 5540 5540 Size: 7411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5219 276 Size: 53 83 186 936 3685 2468 Fragments arranged by size: 3685 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI