(Circular) (Six-base) MAPSORT of: pFN21ASDA1614.seq Check: 622 from: 1 to: 8299 pFN21ASDA1614 8299 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4059 4059 Size: 8299 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5144 6109 278 Size: 53 83 186 936 3608 965 2468 Fragments arranged by size: 3608 2468 965 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5642 1937 Size: 3705 4594 Acc65I G'GTAC_C Cuts at: 5620 5620 Size: 8299 AclI AA'CG_TT Cuts at: 1563 2949 6426 6799 1563 Size: 1386 3477 373 3063 Fragments arranged by size: 3477 3063 1386 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 4134 4452 6362 7746 8014 1632 Size: 2387 115 318 1910 1384 268 1917 Fragments arranged by size: 2387 1917 1910 1384 318 268 115 AfeI AGC'GCT Cuts at: 2846 5435 2846 Size: 2589 5710 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8102 AflIII A'CryG_T Cuts at: 1203 4927 7198 8175 1203 Size: 3724 2271 977 1327 Fragments arranged by size: 3724 2271 1327 977 AgeI A'CCGG_T Cuts at: 5256 7130 5256 Size: 1874 6425 AhdI GACnn_n'nnGTC Cuts at: 3090 7028 3090 Size: 3938 4361 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3077 3476 1184 Size: 1893 399 6007 Fragments arranged by size: 6007 1893 399 AlwNI CAG_nnn'CTG Cuts at: 2435 3448 4538 4584 4919 4955 6042 7614 2435 Size: 1013 1090 46 335 36 1087 1572 3120 Fragments arranged by size: 3120 1572 1090 1087 1013 335 46 36 ApaI G_GGCC'C Cuts at: 2079 2261 2637 3356 3482 4739 5158 2079 Size: 182 376 719 126 1257 419 5220 Fragments arranged by size: 5220 1257 719 419 376 182 126 ApaLI G'TGCA_C Cuts at: 6356 7512 6356 Size: 1156 7143 ApoI r'AATT_y Cuts at: 1493 5608 5834 5986 8095 1493 Size: 4115 226 152 2109 1697 Fragments arranged by size: 4115 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6853 160 Size: 872 5821 1606 Fragments arranged by size: 5821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8299 AvaI C'yCGr_G Cuts at: 1870 1943 3049 3249 3984 4680 5624 1870 Size: 73 1106 200 735 696 944 4545 Fragments arranged by size: 4545 1106 944 735 696 200 73 AvrII C'CTAG_G Cuts at: 4055 4055 Size: 8299 BamHI G'GATC_C Cuts at: 3801 5629 5996 3801 Size: 1828 367 6104 Fragments arranged by size: 6104 1828 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3053 3598 3607 3627 3679 Size: 325 217 627 1266 545 9 20 52 Cuts at: 3679 4441 4632 5411 5481 5620 7075 618 Size: 762 191 779 70 139 1455 1842 Fragments arranged by size: 1842 1455 1266 779 762 627 545 325 217 191 139 70 52 20 9 BanII G_rGCy'C Cuts at: 1391 2079 2261 2576 2637 2657 2966 3119 3356 Size: 688 182 315 61 20 309 153 237 Cuts at: 3356 3482 3526 3711 3755 3783 3977 4023 4698 Size: 126 44 185 44 28 194 46 675 Cuts at: 4698 4739 5158 5618 1391 Size: 41 419 460 4072 Fragments arranged by size: 4072 688 675 460 419 315 309 237 194 185 182 153 126 61 46 44 44 41 28 20 BbeI G_GCGC'C Cuts at: 3683 4445 5485 3683 Size: 762 1040 6497 Fragments arranged by size: 6497 1040 762 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7328 BbvCI CC'TCA_GC Cuts at: 2119 4011 5514 2119 Size: 1892 1503 4904 Fragments arranged by size: 4904 1892 1503 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 6490 6524 1903 Size: 34 1694 34 2825 34 3678 Fragments arranged by size: 3678 2825 1694 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 6191 7401 4556 Size: 1635 1210 5454 Fragments arranged by size: 5454 1635 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8299 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2470 3231 6910 Size: 107 122 71 631 605 798 761 3679 Cuts at: 6910 136 Size: 1525 Fragments arranged by size: 3679 1525 798 761 631 605 122 107 71 BglII A'GATC_T Cuts at: 2779 8294 2779 Size: 5515 2784 BlpI GC'TnA_GC Cuts at: 3113 3449 4807 5240 5716 3113 Size: 336 1358 433 476 5696 Fragments arranged by size: 5696 1358 476 433 336 Bme1580I G_kGCm'C Cuts at: 1790 2079 2261 2637 3056 3157 3207 3300 3339 Size: 289 182 376 419 101 50 93 39 Cuts at: 3339 3356 3482 3836 4739 5158 5338 5450 6360 Size: 17 126 354 903 419 180 112 910 Cuts at: 6360 7516 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 910 903 419 419 376 354 289 182 180 126 112 101 93 50 39 17 BmgBI CAC'GTC Cuts at: 1318 2552 5353 1318 Size: 1234 2801 4264 Fragments arranged by size: 4264 2801 1234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3052 4180 6030 Size: 416 53 83 399 24 1635 1128 1850 Cuts at: 6030 6988 442 Size: 958 1753 Fragments arranged by size: 1850 1753 1635 1128 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8299 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 2267 3302 3865 2134 Size: 133 1035 563 6568 Fragments arranged by size: 6568 1035 563 133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2568 2869 2877 2885 3272 3298 3437 Size: 998 331 301 8 8 387 26 139 Cuts at: 3437 3686 3695 3961 4178 4787 4941 6959 1239 Size: 249 9 266 217 609 154 2018 2579 Fragments arranged by size: 2579 2018 998 609 387 331 301 266 249 217 154 139 26 9 8 8 Bpu10I CC'TnA_GC Cuts at: 2119 2142 3749 4011 4514 5514 5537 2119 Size: 23 1607 262 503 1000 23 4881 Fragments arranged by size: 4881 1607 1000 503 262 23 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6424 7289 7587 6424 Size: 865 298 7136 Fragments arranged by size: 7136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2853 6962 882 Size: 617 1354 4109 2219 Fragments arranged by size: 4109 2219 1354 617 BsaAI yAC'GTr Cuts at: 493 1204 3370 5596 6090 493 Size: 711 2166 2226 494 2702 Fragments arranged by size: 2702 2226 2166 711 494 BsaBI GATnn'nnATC Cuts at: 8293 8293 Size: 8299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3680 4283 4442 5141 Size: 53 83 186 936 2147 603 159 699 Cuts at: 5141 5482 6106 6488 7123 275 Size: 341 624 382 635 1451 Fragments arranged by size: 2147 1451 936 699 635 624 603 382 341 186 159 83 53 BsaWI w'CCGG_w Cuts at: 4753 5256 6732 7130 7404 7551 7962 4753 Size: 503 1476 398 274 147 411 5090 Fragments arranged by size: 5090 1476 503 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3750 3780 3813 3843 4856 Size: 30 165 30 2347 30 33 30 1013 Cuts at: 4856 4886 4914 4944 1178 Size: 30 28 30 4533 Fragments arranged by size: 4533 2347 1013 165 33 30 30 30 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2436 2510 2822 3637 3914 4877 5496 1186 Size: 1250 74 312 815 277 963 619 3989 Fragments arranged by size: 3989 1250 963 815 619 312 277 74 BseYI C'CCAG_C Cuts at: 2223 2629 2636 2723 2836 2879 3244 3739 3924 Size: 406 7 87 113 43 365 495 185 Cuts at: 3924 4041 4207 4437 4519 4608 4613 5083 5363 Size: 117 166 230 82 89 5 470 280 Cuts at: 5363 5526 7502 7998 2223 Size: 163 1976 496 2524 Fragments arranged by size: 2524 1976 496 495 470 406 365 280 230 185 166 163 117 113 89 87 82 43 7 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2997 5159 5319 1163 Size: 573 1261 2162 160 4143 Fragments arranged by size: 4143 2162 1261 573 160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5749 6510 7538 1540 Size: 275 186 3748 761 1028 2301 Fragments arranged by size: 3748 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3455 3711 3755 4023 5362 6360 6445 7516 3455 Size: 256 44 268 1339 998 85 1071 4238 Fragments arranged by size: 4238 1339 1071 998 268 256 85 44 BsmI GAATG_Cn' Cuts at: 5810 5903 5810 Size: 93 8206 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2261 2576 2637 2657 2966 3056 Size: 399 289 182 315 61 20 309 90 Cuts at: 3056 3119 3157 3207 3300 3339 3356 3455 3482 Size: 63 38 50 93 39 17 99 27 Cuts at: 3482 3526 3711 3755 3783 3836 3977 4023 4698 Size: 44 185 44 28 53 141 46 675 Cuts at: 4698 4739 5158 5338 5362 5450 5618 6360 6445 Size: 41 419 180 24 88 168 742 85 Cuts at: 6445 7516 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 675 419 399 315 309 289 185 182 180 168 141 99 93 90 88 85 63 61 53 50 46 44 44 41 39 38 28 27 24 20 17 BspEI T'CCGG_A Cuts at: 7962 7962 Size: 8299 BspHI T'CATG_A Cuts at: 6188 7918 6188 Size: 1730 6569 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2469 4059 5654 844 Size: 1625 1590 1595 3489 Fragments arranged by size: 3489 1625 1595 1590 BsrBI CCG'CTC Cuts at: 1394 1823 3421 6186 1394 Size: 429 1598 2765 3507 Fragments arranged by size: 3507 2765 1598 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6794 6968 65 Size: 1183 5546 174 1396 Fragments arranged by size: 5546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3604 5256 5311 6943 7130 1700 Size: 110 1794 1652 55 1632 187 2869 Fragments arranged by size: 2869 1794 1652 1632 187 110 55 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8299 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8299 BssSI C'ACGA_G Cuts at: 3301 4331 4715 6359 7371 3301 Size: 1030 384 1644 1012 4229 Fragments arranged by size: 4229 1644 1030 1012 384 BstAPI GCAn_nnn'nTGC Cuts at: 794 2941 3145 4051 4639 4817 794 Size: 2147 204 906 588 178 4276 Fragments arranged by size: 4276 2147 906 588 204 178 BstBI TT'CG_AA Cuts at: 2007 7103 2007 Size: 5096 3203 BstEII G'GTnAC_C Cuts at: 1184 4523 1184 Size: 3339 4960 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2178 2215 2107 Size: 71 37 8191 Fragments arranged by size: 8191 71 37 BstYI r'GATC_y Cuts at: 1972 2779 3801 4916 5629 5996 6381 6398 7850 Size: 807 1022 1115 713 367 385 17 1452 Cuts at: 7850 7936 7948 8294 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1115 1022 807 713 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2190 4623 2190 Size: 2433 5866 BtgI C'CryG_G Cuts at: 513 1065 2022 2055 2106 2576 2662 3468 4833 Size: 552 957 33 51 470 86 806 1365 Cuts at: 4833 5327 5379 5521 513 Size: 494 52 142 3291 Fragments arranged by size: 3291 1365 957 806 552 494 470 142 86 52 51 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7560 Fragments arranged by size: 7560 683 56 BtsI GCAGTG_nn' Cuts at: 2893 4637 5815 6609 6629 2893 Size: 1744 1178 794 20 4563 Fragments arranged by size: 4563 1744 1178 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8299 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8264 DraI TTT'AAA Cuts at: 5603 5603 Size: 8299 DraIII CAC_nnn'GTG Cuts at: 3831 5182 5333 3831 Size: 1351 151 6797 Fragments arranged by size: 6797 1351 151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2740 5146 7306 809 Size: 507 1424 2406 2160 1802 Fragments arranged by size: 2406 2160 1802 1424 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2310 3073 Size: 54 1425 50 275 18 216 264 763 Cuts at: 3073 3232 5313 5746 6635 8264 8 Size: 159 2081 433 889 1629 43 Fragments arranged by size: 2081 1629 1425 889 763 433 275 264 216 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5746 1537 Size: 275 3934 4090 Fragments arranged by size: 4090 3934 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5054 5514 6229 1353 Size: 3701 460 715 3423 Fragments arranged by size: 3701 3423 715 460 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3379 3952 4031 6886 7260 7406 Size: 1064 138 1790 573 79 2855 374 146 Cuts at: 7406 387 Size: 1280 Fragments arranged by size: 2855 1790 1280 1064 573 374 146 138 79 EcoICRI GAG'CTC Cuts at: 3709 3753 4021 3709 Size: 44 268 7987 Fragments arranged by size: 7987 268 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2568 2869 2877 2885 3272 3298 Size: 393 605 331 301 8 8 387 26 Cuts at: 3298 3437 3686 3695 3961 4019 4134 4178 4452 Size: 139 249 9 266 58 115 44 274 Cuts at: 4452 4787 4941 6362 6959 7746 8014 1239 Size: 335 154 1421 597 787 268 1524 Fragments arranged by size: 1524 1421 787 605 597 393 387 335 331 301 274 268 266 249 154 139 115 58 44 26 9 8 8 EcoNI CCTnn'n_nnAGG Cuts at: 4200 5393 4200 Size: 1193 7106 EcoO109I rG'GnC_Cy Cuts at: 2075 2115 2257 2258 2519 2633 2690 2742 3171 Size: 40 142 1 261 114 57 52 429 Cuts at: 3171 3253 3352 3353 3478 3479 3696 3909 4030 Size: 82 99 1 125 1 217 213 121 Cuts at: 4030 4181 4581 4735 4736 4787 4964 5154 5155 Size: 151 400 154 1 51 177 190 1 Cuts at: 5155 5553 2075 Size: 398 4821 Fragments arranged by size: 4821 429 400 398 261 217 213 190 177 154 151 142 125 121 114 99 82 57 52 51 40 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 5608 5986 5608 Size: 378 7921 FseI GG_CCGG'CC Cuts at: 5315 5315 Size: 8299 FspI TGC'GCA Cuts at: 6805 6805 Size: 8299 HaeII r_GCGC'y Cuts at: 1678 2848 2880 3436 3442 3683 4445 5437 5485 Size: 1170 32 556 6 241 762 992 48 Cuts at: 5485 7446 1678 Size: 1961 2531 Fragments arranged by size: 2531 1961 1170 992 762 556 241 48 32 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2777 2809 3032 Size: 32 135 32 767 32 406 32 223 Cuts at: 3032 3064 3076 3108 3371 3403 4856 4888 6940 Size: 32 12 32 263 32 1453 32 2052 Cuts at: 6940 6972 7014 7046 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2052 1453 767 406 263 223 135 42 32 32 32 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 5643 5889 669 Size: 988 281 3705 246 3079 Fragments arranged by size: 3705 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8299 HpaI GTT'AAC Cuts at: 5889 5889 Size: 8299 KasI G'GCGC_C Cuts at: 3679 4441 5481 3679 Size: 762 1040 6497 Fragments arranged by size: 6497 1040 762 KpnI G_GTAC'C Cuts at: 5624 5624 Size: 8299 MfeI C'AATT_G Cuts at: 5898 5898 Size: 8299 MluI A'CGCG_T Cuts at: 8175 8175 Size: 8299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5183 7413 7597 1305 Size: 3878 2230 184 2007 Fragments arranged by size: 3878 2230 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2312 3234 10 Size: 54 1425 343 480 922 5075 Fragments arranged by size: 5075 1425 922 480 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6257 6616 6775 518 Size: 648 5091 359 159 2042 Fragments arranged by size: 5091 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2123 2152 2476 2626 3011 3318 3330 Size: 451 66 29 324 150 385 307 12 Cuts at: 3330 3470 3645 5303 5422 5428 5715 6392 7540 Size: 140 175 1658 119 6 287 677 1148 Cuts at: 7540 7785 1606 Size: 245 2120 Fragments arranged by size: 2120 1658 1148 677 451 385 324 307 287 245 175 150 140 119 66 29 12 6 NaeI GCC'GGC Cuts at: 3606 5313 3606 Size: 1707 6592 NarI GG'CG_CC Cuts at: 3680 4442 5482 3680 Size: 762 1040 6497 Fragments arranged by size: 6497 1040 762 NcoI C'CATG_G Cuts at: 513 1065 2022 2662 5521 513 Size: 552 957 640 2859 3291 Fragments arranged by size: 3291 2859 957 640 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8299 NgoMIV G'CCGG_C Cuts at: 3604 5311 3604 Size: 1707 6592 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8299 NotI GC'GGCC_GC Cuts at: 5746 5746 Size: 8299 NspI r_CATG'y Cuts at: 1132 1229 4931 5657 7202 1132 Size: 97 3702 726 1545 2229 Fragments arranged by size: 3702 2229 1545 726 97 PasI CC'CwG_GG Cuts at: 1348 2232 3217 3670 4177 4792 5032 5059 5441 Size: 884 985 453 507 615 240 27 382 Cuts at: 5441 1348 Size: 4206 Fragments arranged by size: 4206 985 884 615 507 453 382 240 27 PciI A'CATG_T Cuts at: 4927 7198 4927 Size: 2271 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 2238 1108 Size: 1130 7169 PfoI T'CCnGG_A Cuts at: 2674 3534 3702 3804 4238 2674 Size: 860 168 102 434 6735 Fragments arranged by size: 6735 860 434 168 102 PmeI GTTT'AAAC Cuts at: 5603 5603 Size: 8299 PmlI CAC'GTG Cuts at: 3370 3370 Size: 8299 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6368 6400 7881 7913 6368 Size: 32 1481 32 6754 Fragments arranged by size: 6754 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2115 2690 2742 3171 3253 3696 3909 4181 4581 Size: 575 52 429 82 443 213 272 400 Cuts at: 4581 4787 4964 2115 Size: 206 177 5450 Fragments arranged by size: 5450 575 443 429 400 272 213 206 177 82 52 PshAI GACnn'nnGTC Cuts at: 1613 2619 1613 Size: 1006 7293 PsiI TTA'TAA Cuts at: 5869 5869 Size: 8299 PspOMI G'GGCC_C Cuts at: 2075 2257 2633 3352 3478 4735 5154 2075 Size: 182 376 719 126 1257 419 5220 Fragments arranged by size: 5220 1257 719 419 376 182 126 PspXI vC'TCGA_Gb Cuts at: 3984 3984 Size: 8299 PstI C_TGCA'G Cuts at: 830 2937 3150 3278 3429 3778 3887 4692 4745 Size: 2107 213 128 151 349 109 805 53 Cuts at: 4745 4902 5427 5433 5651 830 Size: 157 525 6 218 3478 Fragments arranged by size: 3478 2107 805 525 349 218 213 157 151 128 109 53 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8299 PvuII CAG'CTG Cuts at: 2123 2152 2476 3011 3645 5303 5422 5428 2123 Size: 29 324 535 634 1658 119 6 4994 Fragments arranged by size: 4994 1658 634 535 324 119 29 6 SacI G_AGCT'C Cuts at: 3711 3755 4023 3711 Size: 44 268 7987 Fragments arranged by size: 7987 268 44 SacII CC_GC'GG Cuts at: 2058 3471 2058 Size: 1413 6886 SalI G'TCGA_C Cuts at: 1936 5641 1936 Size: 3705 4594 SanDI GG'GwC_CC Cuts at: 3253 4181 3253 Size: 928 7371 SapI GCTCTTCn'nnn_ Cuts at: 5054 5054 Size: 8299 SbfI CC_TGCA'GG Cuts at: 5651 5651 Size: 8299 ScaI AGT'ACT Cuts at: 2558 6547 8259 2558 Size: 3989 1712 2598 Fragments arranged by size: 3989 2598 1712 SexAI A'CCwGG_T Cuts at: 5271 5271 Size: 8299 SfcI C'TryA_G Cuts at: 826 1045 2933 3146 3274 3425 3774 3883 4688 Size: 219 1888 213 128 151 349 109 805 Cuts at: 4688 4741 4898 5101 5423 5429 5647 6782 7463 Size: 53 157 203 322 6 218 1135 681 Cuts at: 7463 7654 826 Size: 191 1471 Fragments arranged by size: 1888 1471 1135 805 681 349 322 219 218 213 203 191 157 151 128 109 53 6 SfoI GGC'GCC Cuts at: 3681 4443 5483 3681 Size: 762 1040 6497 Fragments arranged by size: 6497 1040 762 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8299 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8299 SmaI CCC'GGG Cuts at: 1872 3251 5626 1872 Size: 1379 2375 4545 Fragments arranged by size: 4545 2375 1379 SmlI C'TyrA_G Cuts at: 820 1017 1943 3984 6403 7304 7566 820 Size: 197 926 2041 2419 901 262 1553 Fragments arranged by size: 2419 2041 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5596 6090 493 Size: 5103 494 2702 Fragments arranged by size: 5103 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8299 SphI G_CATG'C Cuts at: 1132 5657 1132 Size: 4525 3774 SspI AAT'ATT Cuts at: 5 52 6223 5 Size: 47 6171 2081 Fragments arranged by size: 6171 2081 47 StuI AGG'CCT Cuts at: 1517 3635 3982 1517 Size: 2118 347 5834 Fragments arranged by size: 5834 2118 347 StyI C'CwwG_G Cuts at: 513 1065 2022 2071 2277 2662 2729 3943 4025 Size: 552 957 49 206 385 67 1214 82 Cuts at: 4025 4055 5521 5738 513 Size: 30 1466 217 3074 Fragments arranged by size: 3074 1466 1214 957 552 385 217 206 82 67 49 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2828 3731 3905 4511 4650 5345 6326 Size: 1255 1248 903 174 606 139 695 981 Cuts at: 6326 6343 6496 6681 7126 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1248 981 903 695 606 445 185 174 153 139 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2245 2556 6545 Size: 274 80 33 51 1443 268 311 3989 Cuts at: 6545 8257 96 Size: 1712 138 Fragments arranged by size: 3989 1712 1443 311 274 268 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3750 3782 3813 3845 3880 3912 3983 4015 4914 Size: 32 31 32 35 32 71 32 899 Cuts at: 4914 4946 8259 8291 3750 Size: 32 3313 32 3758 Fragments arranged by size: 3758 3313 899 71 35 32 32 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 4909 1316 Size: 3593 4706 XbaI T'CTAG_A Cuts at: 5635 5635 Size: 8299 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2592 3719 4193 4401 4799 5269 5300 2592 Size: 1127 474 208 398 470 31 5591 Fragments arranged by size: 5591 1127 474 470 398 208 31 XhoI C'TCGA_G Cuts at: 1943 3984 1943 Size: 2041 6258 XmaI C'CCGG_G Cuts at: 1870 3249 5624 1870 Size: 1379 2375 4545 Fragments arranged by size: 4545 2375 1379 XmnI GAAnn'nnTTC Cuts at: 6428 6428 Size: 8299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5142 6107 276 Size: 53 83 186 936 3608 965 2468 Fragments arranged by size: 3608 2468 965 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PsrI RsrII SfiI SrfI SwaI