(Circular) (Six-base) MAPSORT of: pFN21ASDA1609.seq Check: 4387 from: 1 to: 6097 pFN21ASDA1609 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2500 2500 Size: 6097 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2241 3440 1937 Size: 304 1199 4594 Fragments arranged by size: 4594 1199 304 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 4160 5544 5812 1632 Size: 551 1977 1384 268 1917 Fragments arranged by size: 1977 1917 1384 551 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 2277 3057 4996 5973 1203 Size: 1074 780 1939 977 1327 Fragments arranged by size: 1939 1327 1074 977 780 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 2606 2638 2044 Size: 32 530 32 5503 Fragments arranged by size: 5503 530 32 32 AlwNI CAG_nnn'CTG Cuts at: 2461 2515 2581 3328 3840 5412 2461 Size: 54 66 747 512 1572 3146 Fragments arranged by size: 3146 1572 747 512 66 54 ApaI G_GGCC'C Cuts at: 2496 2574 2895 2496 Size: 78 321 5698 Fragments arranged by size: 5698 321 78 ApaLI G'TGCA_C Cuts at: 2435 3181 4154 5310 2435 Size: 746 973 1156 3222 Fragments arranged by size: 3222 1156 973 746 ApoI r'AATT_y Cuts at: 1493 3406 3632 3784 5893 1493 Size: 1913 226 152 2109 1697 Fragments arranged by size: 2109 1913 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2502 2799 2909 3422 1870 Size: 73 559 297 110 513 4545 Fragments arranged by size: 4545 559 513 297 110 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2811 2820 3418 4873 618 Size: 325 217 627 1024 9 598 1455 1842 Fragments arranged by size: 1842 1455 1024 627 598 325 217 9 BanII G_rGCy'C Cuts at: 1391 2496 2574 2844 2895 3369 3416 1391 Size: 1105 78 270 51 474 47 4072 Fragments arranged by size: 4072 1105 474 270 78 51 47 BbeI G_GCGC'C Cuts at: 2824 2824 Size: 6097 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 2095 1548 Size: 547 5550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2578 4708 136 Size: 107 122 71 631 605 906 2130 1525 Fragments arranged by size: 2130 1525 906 631 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 2444 2523 3514 2444 Size: 79 991 5027 Fragments arranged by size: 5027 991 79 Bme1580I G_kGCm'C Cuts at: 1790 2439 2496 2574 2895 3185 4158 5314 1790 Size: 649 57 78 321 290 973 1156 2573 Fragments arranged by size: 2573 1156 973 649 321 290 78 57 BmgBI CAC'GTC Cuts at: 1318 2182 1318 Size: 864 5233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2262 2484 2569 Size: 416 53 83 399 24 845 222 85 Cuts at: 2569 2626 3107 3828 4786 442 Size: 57 481 721 958 1753 Fragments arranged by size: 1753 958 845 721 481 416 399 222 85 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3353 4757 1239 Size: 2114 1404 2579 Fragments arranged by size: 2579 2114 1404 Bpu10I CC'TnA_GC Cuts at: 2718 3213 2718 Size: 495 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3252 4760 882 Size: 617 1753 1508 2219 Fragments arranged by size: 2219 1753 1508 617 BsaAI yAC'GTr Cuts at: 493 1204 2440 3180 3189 3394 3888 493 Size: 711 1236 740 9 205 494 2702 Fragments arranged by size: 2702 1236 740 711 494 205 9 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2821 3904 4286 4921 Size: 53 83 186 936 1288 1083 382 635 Cuts at: 4921 275 Size: 1451 Fragments arranged by size: 1451 1288 1083 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2044 2074 2843 2873 1178 Size: 30 165 30 641 30 769 30 4402 Fragments arranged by size: 4402 769 641 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 3284 1186 Size: 1162 936 3999 Fragments arranged by size: 3999 1162 936 BseYI C'CCAG_C Cuts at: 2790 2862 3014 5300 5796 2790 Size: 72 152 2286 496 3091 Fragments arranged by size: 3091 2286 496 152 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 1163 Size: 573 792 4732 Fragments arranged by size: 4732 792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 2690 2844 3185 4158 4243 5314 2439 Size: 251 154 341 973 85 1071 3222 Fragments arranged by size: 3222 1071 973 341 251 154 85 BsmI GAATG_Cn' Cuts at: 3346 3608 3701 3346 Size: 262 93 5742 Fragments arranged by size: 5742 262 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2697 2697 Size: 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2496 2574 2690 2844 2895 3185 Size: 399 649 57 78 116 154 51 290 Cuts at: 3185 3369 3416 4158 4243 5314 1391 Size: 184 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 399 290 184 154 116 85 78 57 51 47 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 2346 3986 5716 2346 Size: 1640 1730 2727 Fragments arranged by size: 2727 1730 1640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2500 3452 844 Size: 1656 952 3489 Fragments arranged by size: 3489 1656 952 BsrBI CCG'CTC Cuts at: 1394 1823 3984 1394 Size: 429 2161 3507 Fragments arranged by size: 3507 2161 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2778 3027 96 Size: 2682 249 3166 Fragments arranged by size: 3166 2682 249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 2007 4901 2007 Size: 2894 3203 BstEII G'GTnAC_C Cuts at: 1184 2209 1184 Size: 1025 5072 BstXI CCAn_nnnn'nTGG Cuts at: 2870 2870 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2416 3427 3794 4179 4196 5648 5734 5746 Size: 444 1011 367 385 17 1452 86 12 Cuts at: 5746 6092 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1011 444 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2076 3315 2076 Size: 1239 4858 BtgI C'CryG_G Cuts at: 513 1065 2022 2845 3070 513 Size: 552 957 823 225 3540 Fragments arranged by size: 3540 957 823 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2640 2872 3279 Size: 54 1425 50 275 18 810 232 407 Cuts at: 3279 3544 4433 6062 8 Size: 265 889 1629 43 Fragments arranged by size: 1629 1425 889 810 407 275 265 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2333 4027 1353 Size: 846 134 1694 3423 Fragments arranged by size: 3423 1694 846 134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 4684 5058 5204 387 Size: 1064 138 476 2619 374 146 1280 Fragments arranged by size: 2619 1280 1064 476 374 146 138 EcoICRI GAG'CTC Cuts at: 2842 2842 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 3353 4160 4757 5544 5812 1239 Size: 393 551 1170 807 597 787 268 1524 Fragments arranged by size: 1524 1170 807 787 597 551 393 268 EcoO109I rG'GnC_Cy Cuts at: 2264 2378 2492 2570 2571 2629 2891 2892 3325 Size: 114 114 78 1 58 262 1 433 Cuts at: 3325 2264 Size: 5036 Fragments arranged by size: 5036 433 262 114 114 78 58 1 1 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2824 5244 1678 Size: 1146 2420 2531 Fragments arranged by size: 2531 2420 1146 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 2264 2296 2592 Size: 32 135 32 527 32 133 32 296 Cuts at: 2592 2624 2753 2785 4738 4770 4812 4844 1373 Size: 32 129 32 1953 32 42 32 2626 Fragments arranged by size: 2626 1953 527 296 135 133 129 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 3441 3687 669 Size: 988 281 304 1199 246 3079 Fragments arranged by size: 3079 1199 988 304 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KasI G'GCGC_C Cuts at: 2820 2820 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 2099 3696 2099 Size: 1597 4500 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3235 5211 5395 1305 Size: 1930 1976 184 2007 Fragments arranged by size: 2007 1976 1930 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2642 2874 3281 10 Size: 54 1425 343 810 232 407 2826 Fragments arranged by size: 2826 1425 810 407 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 3023 4055 4414 4573 518 Size: 648 1185 672 1032 359 159 2042 Fragments arranged by size: 2042 1185 1032 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2527 2794 2809 2817 3205 3513 4190 5338 Size: 921 267 15 8 388 308 677 1148 Cuts at: 5338 5583 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 921 677 388 308 267 245 15 8 NarI GG'CG_CC Cuts at: 2821 2821 Size: 6097 NcoI C'CATG_G Cuts at: 513 1065 2022 2845 3070 513 Size: 552 957 823 225 3540 Fragments arranged by size: 3540 957 823 552 225 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2233 2930 3061 3455 5000 1132 Size: 97 1004 697 131 394 1545 2229 Fragments arranged by size: 2229 1545 1004 697 394 131 97 PasI CC'CwG_GG Cuts at: 1348 2489 1348 Size: 1141 4956 PciI A'CATG_T Cuts at: 3057 4996 3057 Size: 1939 4158 PflMI CCAn_nnn'nTGG Cuts at: 1108 2581 1108 Size: 1473 4624 PfoI T'CCnGG_A Cuts at: 2757 3300 3369 3378 2757 Size: 543 69 9 5476 Fragments arranged by size: 5476 543 69 9 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PmlI CAC'GTG Cuts at: 2440 3180 2440 Size: 740 5357 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2044 2076 4166 4198 5679 5711 2044 Size: 32 2090 32 1481 32 2430 Fragments arranged by size: 2430 2090 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2264 2378 2629 2264 Size: 114 251 5732 Fragments arranged by size: 5732 251 114 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2492 2570 2891 2492 Size: 78 321 5698 Fragments arranged by size: 5698 321 78 PspXI vC'TCGA_Gb Cuts at: 2909 2909 Size: 6097 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2527 2794 3205 2527 Size: 267 411 5419 Fragments arranged by size: 5419 411 267 SacI G_AGCT'C Cuts at: 2844 2844 Size: 6097 SalI G'TCGA_C Cuts at: 1936 2240 3439 1936 Size: 304 1199 4594 Fragments arranged by size: 4594 1199 304 SanDI GG'GwC_CC Cuts at: 2264 2629 2264 Size: 365 5732 SapI GCTCTTCn'nnn_ Cuts at: 2199 2199 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2171 3445 4580 5261 5452 826 Size: 219 1126 1274 1135 681 191 1471 Fragments arranged by size: 1471 1274 1135 1126 681 219 191 SfoI GGC'GCC Cuts at: 2822 2822 Size: 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 2504 3424 1872 Size: 632 920 4545 Fragments arranged by size: 4545 920 632 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 4201 5102 5364 820 Size: 197 926 966 1292 901 262 1553 Fragments arranged by size: 1553 1292 966 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3394 3888 493 Size: 2901 494 2702 Fragments arranged by size: 2901 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 2233 3455 1132 Size: 1101 1222 3774 Fragments arranged by size: 3774 1222 1101 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2022 2845 3070 3282 3536 513 Size: 552 957 823 225 212 254 3074 Fragments arranged by size: 3074 957 823 552 254 225 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2608 2613 3124 4124 4141 4294 4479 Size: 1255 1028 5 511 1000 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1028 1000 511 445 185 153 17 5 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2778 3027 3060 Size: 274 80 33 51 1443 801 249 33 Cuts at: 3060 4343 6055 96 Size: 1283 1712 138 Fragments arranged by size: 1712 1443 1283 801 274 249 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2362 2394 2843 2875 6057 6089 2362 Size: 32 449 32 3182 32 2370 Fragments arranged by size: 3182 2370 449 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 2909 1943 Size: 966 5131 XmaI C'CCGG_G Cuts at: 1870 2502 3422 1870 Size: 632 920 4545 Fragments arranged by size: 4545 920 632 XmnI GAAnn'nnTTC Cuts at: 2193 4226 2193 Size: 2033 4064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI