Restriction map for
pFN21ASDA1605
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BbeI
BbrPI BciVI BfrI BglII BsaAI
BsaBI BsaI BsiWI BsmI BspDI
BsrBI BssHII BstBI Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 1813 CGCG CG^CG
AccIII 183 TCCGGA T^CCGGA
AcyI 34 GGCGTC G[GA]^CG[CT]C
AlwNI 1319 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 2292 CCTAGG C^CTAGG
BglI 2389 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2292 CCTAGG C^CTAGG
BlpI 1665 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1665 GCTCAGC GC^T[ACGT]AGC
BsaHI 34 GGCGTC G[GA]^CG[CT]C
BseAI 183 TCCGGA T^CCGGA
BsiEI 1560 CGGTCG CG[GA][CT]^CG
Bsp1407I 1598 TGTACA T^GTACA
BspEI 183 TCCGGA T^CCGGA
BspHI 273 TCATGA T^CATGA
BsrDI 1161 CATTGC GCAATG(2/0)
BsrFI 31 ACCGGC [GA]^CCGG[CT]
BsrGI 1598 TGTACA T^GTACA
BssSI 1016 CACGAG CACGAG(-5/-1)
Bst1107I 1287 GTATAC GTA^TAC
BstAPI 1318 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1813 CGCG CG^CG
BstZ17I 1287 GTATAC GTA^TAC
CelII 1665 GCTCAGC GC^T[ACGT]AGC
Cfr10I 31 ACCGGC [GA]^CCGG[CT]
EcoNI 1503 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1820 ATGCAT ATGCA^T
FauI 944 GCGGG CCCGC(4/6)
HaeII 626 AGCGCC [GA]GCGC^[CT]
Hin1I 34 GGCGTC G[GA]^CG[CT]C
MroI 183 TCCGGA T^CCGGA
MvnI 1813 CGCG CG^CG
NdeI 1607 CATATG CA^TATG
NsiI 1820 ATGCAT ATGCA^T
PaeR7I 1199 CTCGAG C^TCGAG
PshAI 1555 GACTACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 273 TCATGA T^CATGA
SapI 2611 GCTCTTC GCTCTTC(1/4)
SexAI 236 ACCAGGT A^CC[AT]GGT
SspBI 1598 TGTACA T^GTACA
TfiI 1957 GAATC G^A[AT]TC
XbaI 846 TCTAGA T^CTAGA
XhoI 1199 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 1287 GTATAC GT^[AC][GT]AC
2473 GTCTAC
AvaI 698 CCCGAG C^[CT]CG[GA]G
1199 CTCGAG
BamHI 12 GGATCC G^GATCC
72 GGATCC
BbvCI 1329 CCTCAGC CCTCAGC(-5/-2)
2154 CCTCAGC
BcgI 1201 CGAGGCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1878 GCAGGTTTATCG
BclI 419 TGATCA T^GATCA
1908 TGATCA
BseRI 2544 CTCCTC GAGGAG(10/8)
1510 GAGGAG
BsoBI 698 CCCGAG C^[CT]CG[GA]G
1199 CTCGAG
BstEII 1038 GGTAACC G^GT[ACGT]ACC
2037 GGTGACC
BstPI 1038 GGTAACC G^GT[ACGT]ACC
2037 GGTGACC
BstXI 1776 CCATGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2256 CCAGTGTGCTGG
DraIII 570 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1473 CACAGTGTG
EcoO65I 1038 GGTAACC G^GT[ACGT]ACC
2037 GGTGACC
FbaI 419 TGATCA T^GATCA
1908 TGATCA
MseI 524 TTAA T^TAA
1828 TTAA
PflMI 2325 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2549 CCACTTGCTGG
PstI 364 CTGCAG CTGCA^G
1876 CTGCAG
StuI 2282 AGGCCT AGG^CCT
2372 AGGCCT
Tru9I 524 TTAA T^TAA
1828 TTAA
Tsp509I 424 AATT ^AATT
566 AATT
Van91I 2325 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2549 CCACTTGCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
Acc65I AciI AfaI AflIII AluI
AlwI Asp718I AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII