(Circular) (Six-base) MAPSORT of: pFN21ASDA1605.seq Check: 8646 from: 1 to: 7360 pFN21ASDA1605 7360 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2777 3094 3355 2777 Size: 317 261 6782 Fragments arranged by size: 6782 317 261 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5170 278 Size: 53 83 186 936 3634 2468 Fragments arranged by size: 3634 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 4497 4703 1937 Size: 1374 1186 206 4594 Fragments arranged by size: 4594 1374 1186 206 Acc65I G'GTAC_C Cuts at: 2382 2397 3747 4681 2382 Size: 15 1350 934 5061 Fragments arranged by size: 5061 1350 934 15 AclI AA'CG_TT Cuts at: 1563 5487 5860 1563 Size: 3924 373 3063 Fragments arranged by size: 3924 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 3107 3908 3965 4322 4521 4622 5423 Size: 504 971 801 57 357 199 101 801 Cuts at: 5423 6807 7075 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 971 801 801 504 357 268 199 101 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7163 AflIII A'CryG_T Cuts at: 1203 3549 3951 4351 6259 7236 1203 Size: 2346 402 400 1908 977 1327 Fragments arranged by size: 2346 1908 1327 977 402 400 AgeI A'CCGG_T Cuts at: 6191 6191 Size: 7360 AhdI GACnn_n'nnGTC Cuts at: 6089 6089 Size: 7360 AleI CACnn'nnGTG Cuts at: 1166 4334 1166 Size: 3168 4192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7360 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3462 3494 3816 3848 3462 Size: 32 322 32 6974 Fragments arranged by size: 6974 322 32 32 AlwNI CAG_nnn'CTG Cuts at: 3347 5103 6675 3347 Size: 1756 1572 4032 Fragments arranged by size: 4032 1756 1572 ApaLI G'TGCA_C Cuts at: 5417 6573 5417 Size: 1156 6204 ApoI r'AATT_y Cuts at: 1493 4669 4895 5047 7156 1493 Size: 3176 226 152 2109 1697 Fragments arranged by size: 3176 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5914 160 Size: 872 4882 1606 Fragments arranged by size: 4882 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7360 AvaI C'yCGr_G Cuts at: 1870 1943 2721 3222 4685 1870 Size: 73 778 501 1463 4545 Fragments arranged by size: 4545 1463 778 501 73 AvrII C'CTAG_G Cuts at: 4315 4315 Size: 7360 BamHI G'GATC_C Cuts at: 2035 2095 4690 5057 2035 Size: 60 2595 367 4338 Fragments arranged by size: 4338 2595 367 60 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2263 2382 2397 3747 Size: 325 217 627 342 134 119 15 1350 Cuts at: 3747 3769 4645 4681 6136 618 Size: 22 876 36 1455 1842 Fragments arranged by size: 1842 1455 1350 876 627 342 325 217 134 119 36 22 15 BanII G_rGCy'C Cuts at: 1391 2949 3416 3690 4530 4587 4679 1391 Size: 1558 467 274 840 57 92 4072 Fragments arranged by size: 4072 1558 840 467 274 92 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2175 2411 2429 928 Size: 971 276 236 18 5859 Fragments arranged by size: 5859 971 276 236 18 BbvCI CC'TCA_GC Cuts at: 3353 4178 3353 Size: 825 6535 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3213 3247 3890 3924 5551 5585 1903 Size: 34 1276 34 643 34 1627 34 3678 Fragments arranged by size: 3678 1627 1276 643 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5252 6462 5252 Size: 1210 6150 BclI T'GATC_A Cuts at: 1548 2442 3931 1548 Size: 894 1489 4977 Fragments arranged by size: 4977 1489 894 BfrBI ATG'CAT Cuts at: 3845 3845 Size: 7360 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4418 5971 136 Size: 107 122 71 631 605 2746 1553 1525 Fragments arranged by size: 2746 1553 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7355 7355 Size: 7360 BlpI GC'TnA_GC Cuts at: 3689 4777 3689 Size: 1088 6272 Bme1580I G_kGCm'C Cuts at: 1790 4147 5421 6577 1790 Size: 2357 1274 1156 2573 Fragments arranged by size: 2573 2357 1274 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 2915 4068 Size: 416 53 83 399 24 1269 229 1153 Cuts at: 4068 5091 6049 442 Size: 1023 958 1753 Fragments arranged by size: 1753 1269 1153 1023 958 416 399 229 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7360 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3415 3415 Size: 7360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2539 2666 2978 3210 3260 6020 1239 Size: 1300 127 312 232 50 2760 2579 Fragments arranged by size: 2760 2579 1300 312 232 127 50 Bpu10I CC'TnA_GC Cuts at: 2345 3353 4178 2345 Size: 1008 825 5527 Fragments arranged by size: 5527 1008 825 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2398 3536 3620 3704 5485 6350 6648 2398 Size: 1138 84 84 1781 865 298 3110 Fragments arranged by size: 3110 1781 1138 865 298 84 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6023 882 Size: 617 4524 2219 Fragments arranged by size: 4524 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4657 5151 493 Size: 711 3453 494 2702 Fragments arranged by size: 3453 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7354 7354 Size: 7360 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 5167 5549 6184 Size: 53 83 186 936 525 3109 382 635 Cuts at: 6184 275 Size: 1451 Fragments arranged by size: 3109 1451 936 635 525 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2206 2400 2568 5793 6191 6465 6612 7023 2206 Size: 194 168 3225 398 274 147 411 2543 Fragments arranged by size: 3225 2543 411 398 274 194 168 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2354 2820 2850 3483 Size: 30 165 30 921 30 466 30 633 Cuts at: 3483 3513 3816 3846 1178 Size: 30 303 30 4692 Fragments arranged by size: 4692 921 633 466 303 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3548 4558 1186 Size: 2362 1010 3988 Fragments arranged by size: 3988 2362 1010 BseYI C'CCAG_C Cuts at: 2062 3538 4578 6563 7059 2062 Size: 1476 1040 1985 496 2363 Fragments arranged by size: 2363 1985 1476 1040 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 3021 4487 1163 Size: 573 426 859 1466 4036 Fragments arranged by size: 4036 1466 859 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4810 5571 6599 1540 Size: 275 186 1585 1224 761 1028 2301 Fragments arranged by size: 2301 1585 1224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3690 4366 4530 4587 5421 5506 6577 3690 Size: 676 164 57 834 85 1071 4473 Fragments arranged by size: 4473 1071 834 676 164 85 57 BsmI GAATG_Cn' Cuts at: 4871 4964 4871 Size: 93 7267 BsmBI CGTCTCn'nnnn_ Cuts at: 2183 2344 2910 2183 Size: 161 566 6633 Fragments arranged by size: 6633 566 161 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2949 3416 3690 4147 4366 4530 4587 Size: 399 1159 467 274 457 219 164 57 Cuts at: 4587 4679 5421 5506 6577 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1159 1071 742 467 457 399 274 219 164 92 85 57 BspEI T'CCGG_A Cuts at: 2206 7023 2206 Size: 4817 2543 BspHI T'CATG_A Cuts at: 2296 5249 6979 2296 Size: 2953 1730 2677 Fragments arranged by size: 2953 2677 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 2394 2777 3094 3355 3892 4715 844 Size: 1290 260 383 317 261 537 823 3489 Fragments arranged by size: 3489 1290 823 537 383 317 261 260 BsrBI CCG'CTC Cuts at: 1394 1823 5247 1394 Size: 429 3424 3507 Fragments arranged by size: 3507 3424 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3183 5855 6029 65 Size: 1183 1935 2672 174 1396 Fragments arranged by size: 2672 1935 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 6004 6191 1700 Size: 110 244 3950 187 2869 Fragments arranged by size: 3950 2869 244 187 110 BsrGI T'GTAC_A Cuts at: 96 3621 96 Size: 3525 3835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7360 BssSI C'ACGA_G Cuts at: 3039 5420 6432 3039 Size: 2381 1012 3967 Fragments arranged by size: 3967 2381 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3347 794 Size: 2553 4807 BstBI TT'CG_AA Cuts at: 2007 6164 2007 Size: 4157 3203 BstEII G'GTnAC_C Cuts at: 1184 3061 4060 1184 Size: 1877 999 4484 Fragments arranged by size: 4484 1877 999 BstXI CCAn_nnnn'nTGG Cuts at: 3806 4286 3806 Size: 480 6880 BstYI r'GATC_y Cuts at: 1972 2035 2095 3115 3396 4690 5057 5442 5459 Size: 63 60 1020 281 1294 367 385 17 Cuts at: 5459 6911 6997 7009 7355 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1294 1020 385 367 346 281 86 63 60 17 12 BstZ17I GTA'TAC Cuts at: 3312 3312 Size: 7360 BtgI C'CryG_G Cuts at: 513 1065 2022 2512 3057 3827 4413 4441 513 Size: 552 957 490 545 770 586 28 3432 Fragments arranged by size: 3432 957 770 586 552 545 490 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2563 487 Size: 683 56 1337 5284 Fragments arranged by size: 5284 1337 683 56 BtsI GCAGTG_nn' Cuts at: 2396 3434 4876 5670 5690 2396 Size: 1038 1442 794 20 4066 Fragments arranged by size: 4066 1442 1038 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7360 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7325 DraI TTT'AAA Cuts at: 4664 4664 Size: 7360 DraIII CAC_nnn'GTG Cuts at: 2598 3501 2598 Size: 903 6457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6367 809 Size: 507 5051 1802 Fragments arranged by size: 5051 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3151 3325 Size: 54 1425 50 275 18 919 402 174 Cuts at: 3325 3714 4219 4807 5696 7325 8 Size: 389 505 588 889 1629 43 Fragments arranged by size: 1629 1425 919 889 588 505 402 389 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4807 1537 Size: 275 2995 4090 Fragments arranged by size: 4090 2995 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2306 3384 4641 5290 1353 Size: 759 194 1078 1257 649 3423 Fragments arranged by size: 3423 1257 1078 759 649 194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3551 5947 6321 6467 387 Size: 1064 138 1962 2396 374 146 1280 Fragments arranged by size: 2396 1962 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3688 4528 4585 3688 Size: 840 57 6463 Fragments arranged by size: 6463 840 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2539 2666 2978 3107 3210 3260 Size: 393 504 403 127 312 129 103 50 Cuts at: 3260 3908 3965 4322 4521 4622 5423 6020 6807 Size: 648 57 357 199 101 801 597 787 Cuts at: 6807 7075 1239 Size: 268 1524 Fragments arranged by size: 1524 801 787 648 597 504 403 393 357 312 268 199 129 127 103 101 57 50 EcoNI CCTnn'n_nnAGG Cuts at: 3530 3530 Size: 7360 EcoO109I rG'GnC_Cy Cuts at: 2028 2680 3730 3811 3877 4070 2028 Size: 652 1050 81 66 193 5318 Fragments arranged by size: 5318 1050 652 193 81 66 EcoRI G'AATT_C Cuts at: 4669 5047 4669 Size: 378 6982 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4652 4652 Size: 7360 FspI TGC'GCA Cuts at: 5866 5866 Size: 7360 HaeII r_GCGC'y Cuts at: 1678 2653 6507 1678 Size: 975 3854 2531 Fragments arranged by size: 3854 2531 975 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 2722 2754 3416 Size: 32 135 32 448 32 670 32 662 Cuts at: 3416 3448 4117 4149 4229 4261 6001 6033 6075 Size: 32 669 32 80 32 1740 32 42 Cuts at: 6075 6107 1373 Size: 32 2626 Fragments arranged by size: 2626 1740 670 669 662 448 135 80 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4704 4950 669 Size: 988 281 2766 246 3079 Fragments arranged by size: 3079 2766 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7360 HpaI GTT'AAC Cuts at: 4950 4950 Size: 7360 KpnI G_GTAC'C Cuts at: 2386 2401 3751 4685 2386 Size: 15 1350 934 5061 Fragments arranged by size: 5061 1350 934 15 MfeI C'AATT_G Cuts at: 4959 4959 Size: 7360 MluI A'CGCG_T Cuts at: 7236 7236 Size: 7360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3026 6474 6658 1305 Size: 1721 3448 184 2007 Fragments arranged by size: 3448 2007 1721 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2751 3153 3327 3716 10 Size: 54 1425 343 919 402 174 389 3654 Fragments arranged by size: 3654 1425 919 402 389 343 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 3645 4334 5318 5677 5836 518 Size: 648 1272 1207 689 984 359 159 2042 Fragments arranged by size: 2042 1272 1207 984 689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2224 2670 3136 4106 4776 5453 6601 6846 Size: 618 446 466 970 670 677 1148 245 Cuts at: 6846 1606 Size: 2120 Fragments arranged by size: 2120 1148 970 677 670 618 466 446 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3827 4413 4441 513 Size: 552 957 1805 586 28 3432 Fragments arranged by size: 3432 1805 957 586 552 28 NdeI CA'TA_TG Cuts at: 387 3631 387 Size: 3244 4116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7360 NotI GC'GGCC_GC Cuts at: 4807 4807 Size: 7360 NsiI A_TGCA'T Cuts at: 3847 3847 Size: 7360 NspI r_CATG'y Cuts at: 1132 1229 3265 3553 3622 3955 4355 4718 6263 Size: 97 2036 288 69 333 400 363 1545 Cuts at: 6263 1132 Size: 2229 Fragments arranged by size: 2229 2036 1545 400 363 333 288 97 69 PasI CC'CwG_GG Cuts at: 1348 2927 1348 Size: 1579 5781 PciI A'CATG_T Cuts at: 3549 3951 4351 6259 3549 Size: 402 400 1908 4650 Fragments arranged by size: 4650 1908 402 400 PflMI CCAn_nnn'nTGG Cuts at: 1108 4354 4578 1108 Size: 3246 224 3890 Fragments arranged by size: 3890 3246 224 PfoI T'CCnGG_A Cuts at: 4155 4155 Size: 7360 PmeI GTTT'AAAC Cuts at: 4664 4664 Size: 7360 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3816 3848 5429 5461 6942 6974 3816 Size: 32 1581 32 1481 32 4202 Fragments arranged by size: 4202 1581 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2028 3730 3811 3877 2028 Size: 1702 81 66 5511 Fragments arranged by size: 5511 1702 81 66 PshAI GACnn'nnGTC Cuts at: 1613 3582 1613 Size: 1969 5391 PsiI TTA'TAA Cuts at: 4930 4930 Size: 7360 PspXI vC'TCGA_Gb Cuts at: 3222 3222 Size: 7360 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3302 3334 3302 Size: 32 7328 PstI C_TGCA'G Cuts at: 830 2391 3903 4712 830 Size: 1561 1512 809 3478 Fragments arranged by size: 3478 1561 1512 809 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7360 PvuII CAG'CTG Cuts at: 2224 2670 3136 4106 2224 Size: 446 466 970 5478 Fragments arranged by size: 5478 970 466 446 SacI G_AGCT'C Cuts at: 3690 4530 4587 3690 Size: 840 57 6463 Fragments arranged by size: 6463 840 57 SalI G'TCGA_C Cuts at: 1936 4702 1936 Size: 2766 4594 SanDI GG'GwC_CC Cuts at: 2028 3811 2028 Size: 1783 5577 SapI GCTCTTCn'nnn_ Cuts at: 4641 4641 Size: 7360 SbfI CC_TGCA'GG Cuts at: 4712 4712 Size: 7360 ScaI AGT'ACT Cuts at: 5608 7320 5608 Size: 1712 5648 SexAI A'CCwGG_T Cuts at: 2259 2259 Size: 7360 SfcI C'TryA_G Cuts at: 826 1045 2387 2823 3301 3695 3899 4708 5843 Size: 219 1342 436 478 394 204 809 1135 Cuts at: 5843 6524 6715 826 Size: 681 191 1471 Fragments arranged by size: 1471 1342 1135 809 681 478 436 394 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7360 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7360 SmaI CCC'GGG Cuts at: 1872 4687 1872 Size: 2815 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2377 3222 3515 3599 3683 5464 Size: 197 926 434 845 293 84 84 1781 Cuts at: 5464 6365 6627 820 Size: 901 262 1553 Fragments arranged by size: 1781 1553 926 901 845 434 293 262 197 84 84 SnaBI TAC'GTA Cuts at: 493 4657 5151 493 Size: 4164 494 2702 Fragments arranged by size: 4164 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7360 SphI G_CATG'C Cuts at: 1132 4718 1132 Size: 3586 3774 SspI AAT'ATT Cuts at: 5 52 5284 5 Size: 47 5232 2081 Fragments arranged by size: 5232 2081 47 StuI AGG'CCT Cuts at: 1517 4307 4397 1517 Size: 2790 90 4480 Fragments arranged by size: 4480 2790 90 StyI C'CwwG_G Cuts at: 513 1065 2022 2133 2481 3827 4315 4413 4441 Size: 552 957 111 348 1346 488 98 28 Cuts at: 4441 4799 513 Size: 358 3074 Fragments arranged by size: 3074 1346 957 552 488 358 348 111 98 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2474 2800 2897 4048 4474 4478 5387 Size: 1255 894 326 97 1151 426 4 909 Cuts at: 5387 5404 5557 5742 6187 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1151 909 894 445 426 326 185 153 97 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3621 5606 7318 Size: 274 80 33 51 1443 1644 1985 1712 Cuts at: 7318 96 Size: 138 Fragments arranged by size: 1985 1712 1644 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2820 2852 3483 3515 4137 4169 7320 7352 2820 Size: 32 631 32 622 32 3151 32 2828 Fragments arranged by size: 3151 2828 631 622 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7360 XbaI T'CTAG_A Cuts at: 2869 4696 2869 Size: 1827 5533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2934 4049 4621 2934 Size: 1115 572 5673 Fragments arranged by size: 5673 1115 572 XhoI C'TCGA_G Cuts at: 1943 3222 1943 Size: 1279 6081 XmaI C'CCGG_G Cuts at: 1870 4685 1870 Size: 2815 4545 XmnI GAAnn'nnTTC Cuts at: 5489 5489 Size: 7360 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5168 276 Size: 53 83 186 936 3634 2468 Fragments arranged by size: 3634 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII SfiI SfoI SrfI SwaI