Restriction map for
pFN21ASDA1605
(Linear) (Six-base) MAPSORT of: KIAA1605.seq Check: 2858 from: 1 to: 2631
KIAA1605.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 754 1071 1332 2631
Size: 754 317 261 1299
Fragments arranged by size:
1299 754 317 261
AccI GT'mk_AC
Cuts at: 0 1288 2474 2631
Size: 1288 1186 157
Fragments arranged by size:
1288 1186 157
Acc65I G'GTAC_C
Cuts at: 0 359 374 1724 2631
Size: 359 15 1350 907
Fragments arranged by size:
1350 907 359 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1084 1885 1942 2299 2498 2599 2631
Size: 113 971 801 57 357 199 101 32
Fragments arranged by size:
971 801 357 199 113 101 57 32
AflIII A'CryG_T
Cuts at: 0 1526 1928 2328 2631
Size: 1526 402 400 303
Fragments arranged by size:
1526 402 400 303
AleI CACnn'nnGTG
Cuts at: 0 2311 2631
Size: 2311 320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1439 1471 1793 1825 2631
Size: 1439 32 322 32 806
Fragments arranged by size:
1439 806 322 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1324 2631
Size: 1324 1307
AvaI C'yCGr_G
Cuts at: 0 698 1199 2631
Size: 698 501 1432
Fragments arranged by size:
1432 698 501
AvrII C'CTAG_G
Cuts at: 0 2292 2631
Size: 2292 339
BamHI G'GATC_C
Cuts at: 0 12 72 2631
Size: 12 60 2559
Fragments arranged by size:
2559 60 12
BanI G'GyrC_C
Cuts at: 0 106 240 359 374 1724 1746 2622 2631
Size: 106 134 119 15 1350 22 876 9
Fragments arranged by size:
1350 876 134 119 106 22 15 9
BanII G_rGCy'C
Cuts at: 0 926 1393 1667 2507 2564 2631
Size: 926 467 274 840 57 67
Fragments arranged by size:
926 840 467 274 67 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 152 388 406 2631
Size: 152 236 18 2225
Fragments arranged by size:
2225 236 152 18
BbvCI CC'TCA_GC
Cuts at: 0 1330 2155 2631
Size: 1330 825 476
Fragments arranged by size:
1330 825 476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1190 1224 1867 1901 2631
Size: 1190 34 643 34 730
Fragments arranged by size:
1190 730 643 34 34
BclI T'GATC_A
Cuts at: 0 419 1908 2631
Size: 419 1489 723
Fragments arranged by size:
1489 723 419
BfrBI ATG'CAT
Cuts at: 0 1822 2631
Size: 1822 809
BglI GCCn_nnn'nGGC
Cuts at: 0 2395 2631
Size: 2395 236
BlpI GC'TnA_GC
Cuts at: 0 1666 2631
Size: 1666 965
Bme1580I G_kGCm'C
Cuts at: 0 2124 2631
Size: 2124 507
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 892 2045 2631
Size: 663 229 1153 586
Fragments arranged by size:
1153 663 586 229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1392 2631
Size: 1392 1239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 643 955 1187 1237 2631
Size: 516 127 312 232 50 1394
Fragments arranged by size:
1394 516 312 232 127 50
Bpu10I CC'TnA_GC
Cuts at: 0 322 1330 2155 2631
Size: 322 1008 825 476
Fragments arranged by size:
1008 825 476 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 1513 1597 1681 2631
Size: 375 1138 84 84 950
Fragments arranged by size:
1138 950 375 84 84
BsaHI Gr'CG_yC
Cuts at: 0 35 2631
Size: 35 2596
BsaWI w'CCGG_w
Cuts at: 0 183 377 545 2631
Size: 183 194 168 2086
Fragments arranged by size:
2086 194 183 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 797 827 1460 1490 1793 1823
Size: 301 30 466 30 633 30 303 30
Cuts at: 1823 2631
Size: 808
Fragments arranged by size:
808 633 466 303 301 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1525 2535 2631
Size: 1525 1010 96
Fragments arranged by size:
1525 1010 96
BseYI C'CCAG_C
Cuts at: 0 39 1515 2555 2631
Size: 39 1476 1040 76
Fragments arranged by size:
1476 1040 76 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 998 2464 2631
Size: 139 859 1466 167
Fragments arranged by size:
1466 859 167 139
BsiEI CG_ry'CG
Cuts at: 0 1563 2631
Size: 1563 1068
BsiHKAI G_wGCw'C
Cuts at: 0 1667 2343 2507 2564 2631
Size: 1667 676 164 57 67
Fragments arranged by size:
1667 676 164 67 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 321 887 2631
Size: 160 161 566 1744
Fragments arranged by size:
1744 566 161 160
Bsp1286I G_dGCh'C
Cuts at: 0 926 1393 1667 2124 2343 2507 2564 2631
Size: 926 467 274 457 219 164 57 67
Fragments arranged by size:
926 467 457 274 219 164 67 57
BspEI T'CCGG_A
Cuts at: 0 183 2631
Size: 183 2448
BspHI T'CATG_A
Cuts at: 0 273 2631
Size: 273 2358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 371 754 1071 1332 1869 2631
Size: 111 260 383 317 261 537 762
Fragments arranged by size:
762 537 383 317 261 260 111
BsrDI GCAATG_nn'
Cuts at: 0 1160 2631
Size: 1160 1471
BsrFI r'CCGG_y
Cuts at: 0 31 2631
Size: 31 2600
BsrGI T'GTAC_A
Cuts at: 0 1598 2631
Size: 1598 1033
BssSI C'ACGA_G
Cuts at: 0 1016 2631
Size: 1016 1615
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1324 2631
Size: 1324 1307
BstEII G'GTnAC_C
Cuts at: 0 1038 2037 2631
Size: 1038 999 594
Fragments arranged by size:
1038 999 594
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1783 2263 2631
Size: 1783 480 368
Fragments arranged by size:
1783 480 368
BstYI r'GATC_y
Cuts at: 0 12 72 1092 1373 2631
Size: 12 60 1020 281 1258
Fragments arranged by size:
1258 1020 281 60 12
BstZ17I GTA'TAC
Cuts at: 0 1289 2631
Size: 1289 1342
BtgI C'CryG_G
Cuts at: 0 489 1034 1804 2390 2418 2631
Size: 489 545 770 586 28 213
Fragments arranged by size:
770 586 545 489 213 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 2631
Size: 540 2091
BtsI GCAGTG_nn'
Cuts at: 0 373 1411 2631
Size: 373 1038 1220
Fragments arranged by size:
1220 1038 373
DraIII CAC_nnn'GTG
Cuts at: 0 575 1478 2631
Size: 575 903 1153
Fragments arranged by size:
1153 903 575
EaeI y'GGCC_r
Cuts at: 0 726 1128 1302 1691 2196 2631
Size: 726 402 174 389 505 435
Fragments arranged by size:
726 505 435 402 389 174
EarI CTCTTCn'nnn_
Cuts at: 0 89 283 1361 2618 2631
Size: 89 194 1078 1257 13
Fragments arranged by size:
1257 1078 194 89 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1528 2631
Size: 1528 1103
EcoICRI GAG'CTC
Cuts at: 0 1665 2505 2562 2631
Size: 1665 840 57 69
Fragments arranged by size:
1665 840 69 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 516 643 955 1084 1187 1237 1885
Size: 113 403 127 312 129 103 50 648
Cuts at: 1885 1942 2299 2498 2599 2631
Size: 57 357 199 101 32
Fragments arranged by size:
648 403 357 312 199 129 127 113
103 101 57 50 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1507 2631
Size: 1507 1124
EcoO109I rG'GnC_Cy
Cuts at: 0 5 657 1707 1788 1854 2047 2631
Size: 5 652 1050 81 66 193 584
Fragments arranged by size:
1050 652 584 193 81 66 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2629 2631
Size: 2629 2
HaeII r_GCGC'y
Cuts at: 0 630 2631
Size: 630 2001
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 699 731 1393 1425 2094 2126 2206
Size: 29 670 32 662 32 669 32 80
Cuts at: 2206 2238 2631
Size: 32 393
Fragments arranged by size:
670 669 662 393 80 32 32 32
32 29
KpnI G_GTAC'C
Cuts at: 0 363 378 1728 2631
Size: 363 15 1350 903
Fragments arranged by size:
1350 903 363 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 2631
Size: 1003 1628
MscI TGG'CCA
Cuts at: 0 728 1130 1304 1693 2631
Size: 728 402 174 389 938
Fragments arranged by size:
938 728 402 389 174
MslI CAynn'nnrTG
Cuts at: 0 415 1622 2311 2631
Size: 415 1207 689 320
Fragments arranged by size:
1207 689 415 320
MspA1I CmG'CkG
Cuts at: 0 201 647 1113 2083 2631
Size: 201 446 466 970 548
Fragments arranged by size:
970 548 466 446 201
NcoI C'CATG_G
Cuts at: 0 1804 2390 2418 2631
Size: 1804 586 28 213
Fragments arranged by size:
1804 586 213 28
NdeI CA'TA_TG
Cuts at: 0 1608 2631
Size: 1608 1023
NsiI A_TGCA'T
Cuts at: 0 1824 2631
Size: 1824 807
NspI r_CATG'y
Cuts at: 0 1242 1530 1599 1932 2332 2631
Size: 1242 288 69 333 400 299
Fragments arranged by size:
1242 400 333 299 288 69
PasI CC'CwG_GG
Cuts at: 0 904 2631
Size: 904 1727
PciI A'CATG_T
Cuts at: 0 1526 1928 2328 2631
Size: 1526 402 400 303
Fragments arranged by size:
1526 402 400 303
PflMI CCAn_nnn'nTGG
Cuts at: 0 2331 2555 2631
Size: 2331 224 76
Fragments arranged by size:
2331 224 76
PfoI T'CCnGG_A
Cuts at: 0 2132 2631
Size: 2132 499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1793 1825 2631
Size: 1793 32 806
Fragments arranged by size:
1793 806 32
PpuMI rG'GwC_Cy
Cuts at: 0 5 1707 1788 1854 2631
Size: 5 1702 81 66 777
Fragments arranged by size:
1702 777 81 66 5
PshAI GACnn'nnGTC
Cuts at: 0 1559 2631
Size: 1559 1072
PspXI vC'TCGA_Gb
Cuts at: 0 1199 2631
Size: 1199 1432
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1279 1311 2631
Size: 1279 32 1320
Fragments arranged by size:
1320 1279 32
PstI C_TGCA'G
Cuts at: 0 368 1880 2631
Size: 368 1512 751
Fragments arranged by size:
1512 751 368
PvuII CAG'CTG
Cuts at: 0 201 647 1113 2083 2631
Size: 201 446 466 970 548
Fragments arranged by size:
970 548 466 446 201
SacI G_AGCT'C
Cuts at: 0 1667 2507 2564 2631
Size: 1667 840 57 67
Fragments arranged by size:
1667 840 67 57
SanDI GG'GwC_CC
Cuts at: 0 5 1788 2631
Size: 5 1783 843
Fragments arranged by size:
1783 843 5
SapI GCTCTTCn'nnn_
Cuts at: 0 2618 2631
Size: 2618 13
SexAI A'CCwGG_T
Cuts at: 0 236 2631
Size: 236 2395
SfcI C'TryA_G
Cuts at: 0 364 800 1278 1672 1876 2631
Size: 364 436 478 394 204 755
Fragments arranged by size:
755 478 436 394 364 204
SmlI C'TyrA_G
Cuts at: 0 354 1199 1492 1576 1660 2631
Size: 354 845 293 84 84 971
Fragments arranged by size:
971 845 354 293 84 84
StuI AGG'CCT
Cuts at: 0 2284 2374 2631
Size: 2284 90 257
Fragments arranged by size:
2284 257 90
StyI C'CwwG_G
Cuts at: 0 110 458 1804 2292 2390 2418 2631
Size: 110 348 1346 488 98 28 213
Fragments arranged by size:
1346 488 348 213 110 98 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 777 874 2025 2451 2455 2631
Size: 451 326 97 1151 426 4 176
Fragments arranged by size:
1151 451 426 326 176 97 4
TatI w'GTAC_w
Cuts at: 0 1598 2631
Size: 1598 1033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 797 829 1460 1492 2114 2146 2631
Size: 797 32 631 32 622 32 485
Fragments arranged by size:
797 631 622 485 32 32 32
XbaI T'CTAG_A
Cuts at: 0 846 2631
Size: 846 1785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 911 2026 2598 2631
Size: 911 1115 572 33
Fragments arranged by size:
1115 911 572 33
XhoI C'TCGA_G
Cuts at: 0 1199 2631
Size: 1199 1432
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AloI AlwNI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PshAI PspXI PsrI
PstI PvuII SacI SanDI SapI SexAI SfcI SmlI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI ApaI
ApaLI ApoI AscI AseI AsiSI BaeI BbeI BciVI
BglII BmgBI BmtI BsaI BsaAI BsaBI BsiWI BsmI
BsrBI BssHII BstBI Bsu36I ClaI CspCI DraI DrdI
EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PmeI PmlI PsiI PspOMI PvuI
RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XmaI XmnI ZraI