Restriction map for
pFN21ASDA1599
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AvrII BamHI BbeI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI Cfr10I
ClaI CpoI DraI DraIII DrdI
EarI Eco47III EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI RsrII SacII
SalI SapI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 147 GTATAC GT^[AC][GT]AC
AclI 630 AACGTT AA^CGTT
AflIII 113 ACATGT A^C[GA][CT]GT
AhdI 16 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 942 GTGCAC G^TGCAC
AlwNI 516 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 10 CCTGAGG CC^T[ACGT]AGG
ApaLI 942 GTGCAC G^TGCAC
Asp700I 570 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 16 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1552 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 487 GGCACC G^G[CT][GA]CC
BbvCI 505 GCTGAGG CCTCAGC(-5/-2)
BglI 1618 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1246 CTGGAG CTGGAG(16/14)
BsmAI 1631 GAGAC GTCTC(1/5)
BsmBI 1631 GAGACG CGTCTC(1/5)
BspHI 659 TCATGA T^CATGA
BspLU11I 113 ACATGT A^CATGT
BssSI 1186 CACGAG CACGAG(-5/-1)
Bst1107I 147 GTATAC GTA^TAC
BstEII 1311 GGTCACC G^GT[ACGT]ACC
BstPI 1311 GGTCACC G^GT[ACGT]ACC
BstZ17I 147 GTATAC GTA^TAC
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
EagI 1337 CGGCCG C^GGCCG
Eam1105I 16 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1337 CGGCCG C^GGCCG
Eco52I 1337 CGGCCG C^GGCCG
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
EcoNI 333 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1311 GGTCACC G^GT[ACGT]ACC
HaeII 1083 GGCGCT [GA]GCGC^[CT]
NcoI 1490 CCATGG C^CATGG
NspI 113 ACATGT [GA]CATG^[CT]
PflFI 1552 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 630 AACGTT AA^CGTT
PstI 90 CTGCAG CTGCA^G
PvuI 732 CGATCG CGAT^CG
RcaI 659 TCATGA T^CATGA
SacI 511 GAGCTC GAGCT^C
SbfI 89 CCTGCAGG CCTGCA^GG
ScaI 1358 AGTACT AGT^ACT
SfiI 1617 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1529 CCCGGG CCC^GGG
Sse8387I 89 CCTGCAGG CCTGCA^GG
Tth111I 1552 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1529 CCCGGG C^CCGGG
XmaI 1529 CCCGGG C^CCGGG
XmnI 570 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 423 GAATTC [GA]^AATT[CT]
897 GAATTC
AcyI 21 GGCGTC G[GA]^CG[CT]C
1486 GACGCC
ApoI 423 GAATTC [GA]^AATT[CT]
897 GAATTC
AspHI 511 GAGCTC G[AT]GC[AT]^C
942 GTGCAC
AvaI 1529 CCCGGG C^[CT]CG[GA]G
1628 CCCGAG
AviII 230 TGCGCA TGC^GCA
1268 TGCGCA
BalI 992 TGGCCA TGG^CCA
1322 TGGCCA
BbrPI 1318 CACGTG CAC^GTG
1603 CACGTG
BsaHI 21 GGCGTC G[GA]^CG[CT]C
1486 GACGCC
BsiEI 732 CGATCG CG[GA][CT]^CG
1337 CGGCCG
BsiHKAI 511 GAGCTC G[AT]GC[AT]^C
942 GTGCAC
BsoBI 1529 CCCGGG C^[CT]CG[GA]G
1628 CCCGAG
BstYI 1520 GGATCT [GA]^GATC[CT]
1646 AGATCC
DraII 370 GGGTCCC [GA]G^G[ACGT]CC[CT]
970 GGGACCC
EcoO109I 370 GGGTCCC [GA]G^G[ACGT]CC[CT]
970 GGGACCC
EcoRI 423 GAATTC G^AATTC
897 GAATTC
FspI 230 TGCGCA TGC^GCA
1268 TGCGCA
Hin1I 21 GGCGTC G[GA]^CG[CT]C
1486 GACGCC
HincII 295 GTTGAC GT[CT]^[GA]AC
1426 GTCAAC
HindII 295 GTTGAC GT[CT]^[GA]AC
1426 GTCAAC
MboII 663 GAAGA GAAGA(8/7)
879 GAAGA
MflI 1520 GGATCT [GA]^GATC[CT]
1646 AGATCC
MluNI 992 TGGCCA TGG^CCA
1322 TGGCCA
MscI 992 TGGCCA TGG^CCA
1322 TGGCCA
PflMI 153 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1195 CCACTGAATGG
PmaCI 1318 CACGTG CAC^GTG
1603 CACGTG
PmlI 1318 CACGTG CAC^GTG
1603 CACGTG
PpuMI 370 GGGTCCC [GA]G^G[AT]CC[CT]
970 GGGACCC
PshAI 651 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1089 GACTGCCGTC
StuI 341 AGGCCT AGG^CCT
1250 AGGCCT
Van91I 153 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1195 CCACTGAATGG
XcmI 780 CCATGACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1490 CCATGGTGGAGCTGG
XhoII 1520 GGATCT [GA]^GATC[CT]
1646 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BlpI
BmyI Bpu1102I BsaAI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I