(Circular) (Six-base) MAPSORT of: pFN21ASDA1599.seq Check: 2319 from: 1 to: 6508 pFN21ASDA1599 6508 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2171 3851 1937 Size: 234 1680 4594 Fragments arranged by size: 4594 1680 234 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 2654 4635 5008 1563 Size: 1091 1981 373 3063 Fragments arranged by size: 3063 1981 1091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4571 5955 6223 1632 Size: 2939 1384 268 1917 Fragments arranged by size: 2939 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 2136 5407 6384 1203 Size: 933 3271 977 1327 Fragments arranged by size: 3271 1327 977 933 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 2044 5237 2044 Size: 3193 3315 AleI CACnn'nnGTG Cuts at: 1166 2093 3341 1166 Size: 927 1248 4333 Fragments arranged by size: 4333 1248 927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 2589 3644 1184 Size: 1299 106 1055 4048 Fragments arranged by size: 4048 1299 1055 106 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2639 2671 2639 Size: 32 6476 AlwNI CAG_nnn'CTG Cuts at: 2544 4251 5823 2544 Size: 1707 1572 3229 Fragments arranged by size: 3229 1707 1572 ApaLI G'TGCA_C Cuts at: 2965 4565 5721 2965 Size: 1600 1156 3752 Fragments arranged by size: 3752 1600 1156 ApoI r'AATT_y Cuts at: 1493 2446 2920 3817 4043 4195 6304 1493 Size: 953 474 897 226 152 2109 1697 Fragments arranged by size: 2109 1697 953 897 474 226 152 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 3552 3651 3833 1870 Size: 73 1609 99 182 4545 Fragments arranged by size: 4545 1609 182 99 73 BamHI G'GATC_C Cuts at: 3838 4205 3838 Size: 367 6141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2510 3829 5284 618 Size: 325 217 627 723 1319 1455 1842 Fragments arranged by size: 1842 1455 1319 723 627 325 217 BanII G_rGCy'C Cuts at: 1391 2538 2737 3080 3827 1391 Size: 1147 199 343 747 4072 Fragments arranged by size: 4072 1147 747 343 199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5537 BbvCI CC'TCA_GC Cuts at: 2529 2529 Size: 6508 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 1548 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3647 5119 136 Size: 107 122 71 631 605 1975 1472 1525 Fragments arranged by size: 1975 1525 1472 631 605 122 107 71 BglII A'GATC_T Cuts at: 6503 6503 Size: 6508 BlpI GC'TnA_GC Cuts at: 2051 2537 3632 3925 2051 Size: 486 1095 293 4634 Fragments arranged by size: 4634 1095 486 293 Bme1580I G_kGCm'C Cuts at: 1790 2969 4569 5725 1790 Size: 1179 1600 1156 2573 Fragments arranged by size: 2573 1600 1179 1156 BmgBI CAC'GTC Cuts at: 1318 3538 3658 1318 Size: 2220 120 4168 Fragments arranged by size: 4168 2220 120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2794 3373 4239 Size: 416 53 83 399 24 1377 579 866 Cuts at: 4239 5197 442 Size: 958 1753 Fragments arranged by size: 1753 1377 958 866 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2080 3259 2080 Size: 1179 5329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3290 5168 1239 Size: 2051 1878 2579 Fragments arranged by size: 2579 2051 1878 Bpu10I CC'TnA_GC Cuts at: 2529 2529 Size: 6508 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4633 5498 5796 4633 Size: 865 298 5345 Fragments arranged by size: 5345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5171 882 Size: 617 3672 2219 Fragments arranged by size: 3672 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2917 3343 3607 3628 3805 4299 493 Size: 711 1713 426 264 21 177 494 2702 Fragments arranged by size: 2702 1713 711 494 426 264 177 21 BsaBI GATnn'nnATC Cuts at: 6502 6502 Size: 6508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 3510 4315 4697 Size: 53 83 186 936 512 1465 805 382 Cuts at: 4697 5332 275 Size: 635 1451 Fragments arranged by size: 1465 1451 936 805 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4941 5339 5613 5760 6171 4941 Size: 398 274 147 411 5278 Fragments arranged by size: 5278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2373 2403 2948 2978 1178 Size: 30 165 30 970 30 545 30 4708 Fragments arranged by size: 4708 970 545 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2546 3538 1186 Size: 1111 249 992 4156 Fragments arranged by size: 4156 1111 992 249 BseYI C'CCAG_C Cuts at: 3760 5711 6207 3760 Size: 1951 496 4061 Fragments arranged by size: 4061 1951 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 3050 3320 3386 3773 1163 Size: 573 620 694 270 66 387 3898 Fragments arranged by size: 3898 694 620 573 387 270 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2758 3363 3958 4719 5747 1540 Size: 275 186 757 605 595 761 1028 2301 Fragments arranged by size: 2301 1028 761 757 605 595 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 2969 4569 4654 5725 2538 Size: 431 1600 85 1071 3321 Fragments arranged by size: 3321 1600 1071 431 85 BsmI GAATG_Cn' Cuts at: 2435 3358 3708 4019 4112 2435 Size: 923 350 311 93 4831 Fragments arranged by size: 4831 923 350 311 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3648 3648 Size: 6508 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2538 2737 2969 3080 3827 4569 4654 Size: 399 748 199 232 111 747 742 85 Cuts at: 4654 5725 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 748 747 742 399 232 199 111 85 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 2682 4397 6127 2682 Size: 1715 1730 3063 Fragments arranged by size: 3063 1730 1715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3863 844 Size: 3019 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4395 1394 Size: 429 2572 3507 Fragments arranged by size: 3507 2572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5003 5177 65 Size: 1183 3755 174 1396 Fragments arranged by size: 3755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5152 5339 1700 Size: 110 3342 187 2869 Fragments arranged by size: 3342 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6508 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 3209 4568 5580 3209 Size: 1359 1012 4137 Fragments arranged by size: 4137 1359 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6508 BstBI TT'CG_AA Cuts at: 2007 5312 2007 Size: 3305 3203 BstEII G'GTnAC_C Cuts at: 1184 3334 1184 Size: 2150 4358 BstXI CCAn_nnnn'nTGG Cuts at: 2095 2584 2770 2095 Size: 489 186 5833 Fragments arranged by size: 5833 489 186 BstYI r'GATC_y Cuts at: 1972 3543 3669 3838 4205 4590 4607 6059 6145 Size: 1571 126 169 367 385 17 1452 86 Cuts at: 6145 6157 6503 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1571 1452 385 367 346 169 126 86 17 12 BstZ17I GTA'TAC Cuts at: 2172 2172 Size: 6508 Bsu36I CC'TnA_GG Cuts at: 2034 2034 Size: 6508 BtgI C'CryG_G Cuts at: 513 1065 2022 2848 3330 3513 513 Size: 552 957 826 482 183 3508 Fragments arranged by size: 3508 957 826 552 482 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 2201 3027 4024 4818 4838 2201 Size: 826 997 794 20 3871 Fragments arranged by size: 3871 997 826 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3304 3339 529 Size: 35 2740 35 3698 Fragments arranged by size: 3698 2740 35 35 DraI TTT'AAA Cuts at: 3812 3812 Size: 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 3015 3345 Size: 54 1425 50 275 18 255 930 330 Cuts at: 3345 3360 3955 4844 6473 8 Size: 15 595 889 1629 43 Fragments arranged by size: 1629 1425 930 889 595 330 275 255 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3360 3955 1537 Size: 275 1548 595 4090 Fragments arranged by size: 4090 1548 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4438 1353 Size: 3085 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5095 5469 5615 387 Size: 1064 138 3506 374 146 1280 Fragments arranged by size: 3506 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2536 2536 Size: 6508 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3290 4571 5168 5955 6223 1239 Size: 393 1658 1281 597 787 268 1524 Fragments arranged by size: 1658 1524 1281 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 6508 EcoO109I rG'GnC_Cy Cuts at: 2394 2994 2394 Size: 600 5908 EcoRI G'AATT_C Cuts at: 2446 2920 3817 4195 2446 Size: 474 897 378 4759 Fragments arranged by size: 4759 897 474 378 FspI TGC'GCA Cuts at: 2255 3293 5014 2255 Size: 1038 1721 3749 Fragments arranged by size: 3749 1721 1038 HaeII r_GCGC'y Cuts at: 1678 3110 5655 1678 Size: 1432 2545 2531 Fragments arranged by size: 2545 2531 1432 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2306 2338 3114 Size: 32 135 32 458 32 244 32 776 Cuts at: 3114 3146 5149 5181 5223 5255 1373 Size: 32 2003 32 42 32 2626 Fragments arranged by size: 2626 2003 776 458 244 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 3451 3852 4098 669 Size: 988 281 382 1131 401 246 3079 Fragments arranged by size: 3079 1131 988 401 382 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6508 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 4107 4107 Size: 6508 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2175 2505 3100 5622 5806 1305 Size: 870 330 595 2522 184 2007 Fragments arranged by size: 2522 2007 870 595 330 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 3347 10 Size: 54 1425 343 1185 330 3171 Fragments arranged by size: 3171 1425 1185 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2726 2768 3341 4466 4825 4984 Size: 648 927 633 42 573 1125 359 159 Cuts at: 4984 518 Size: 2042 Fragments arranged by size: 2042 1125 927 648 633 573 359 159 42 MspA1I CmG'CkG Cuts at: 1606 2474 2528 3088 3304 3359 3924 4601 5749 Size: 868 54 560 216 55 565 677 1148 Cuts at: 5749 5994 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 868 677 565 560 245 216 55 54 NcoI C'CATG_G Cuts at: 513 1065 2022 3513 513 Size: 552 957 1491 3508 Fragments arranged by size: 3508 1491 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 2140 3866 5411 1132 Size: 97 911 1726 1545 2229 Fragments arranged by size: 2229 1726 1545 911 97 PasI CC'CwG_GG Cuts at: 1348 2399 3166 1348 Size: 1051 767 4690 Fragments arranged by size: 4690 1051 767 PciI A'CATG_T Cuts at: 2136 5407 2136 Size: 3271 3237 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 3224 1108 Size: 1074 1042 4392 Fragments arranged by size: 4392 1074 1042 PfoI T'CCnGG_A Cuts at: 2124 2450 3267 3597 2124 Size: 326 817 330 5035 Fragments arranged by size: 5035 817 330 326 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PmlI CAC'GTG Cuts at: 3343 3628 3343 Size: 285 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 6090 6122 4577 Size: 32 1481 32 4963 Fragments arranged by size: 4963 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2394 2994 2394 Size: 600 5908 PshAI GACnn'nnGTC Cuts at: 1613 2678 3116 1613 Size: 1065 438 5005 Fragments arranged by size: 5005 1065 438 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PstI C_TGCA'G Cuts at: 830 2117 3860 830 Size: 1287 1743 3478 Fragments arranged by size: 3478 1743 1287 PvuI CG_AT'CG Cuts at: 2001 2758 2001 Size: 757 5751 PvuII CAG'CTG Cuts at: 2474 3088 3304 2474 Size: 614 216 5678 Fragments arranged by size: 5678 614 216 SacI G_AGCT'C Cuts at: 2538 2538 Size: 6508 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SanDI GG'GwC_CC Cuts at: 2394 2994 2394 Size: 600 5908 SbfI CC_TGCA'GG Cuts at: 2117 3860 2117 Size: 1743 4765 ScaI AGT'ACT Cuts at: 3383 4756 6468 3383 Size: 1373 1712 3423 Fragments arranged by size: 3423 1712 1373 SfcI C'TryA_G Cuts at: 826 1045 2113 2545 2632 3856 4991 5672 5863 Size: 219 1068 432 87 1224 1135 681 191 Cuts at: 5863 826 Size: 1471 Fragments arranged by size: 1471 1224 1135 1068 681 432 219 191 87 SfiI GGCCn_nnn'nGGCC Cuts at: 3647 3647 Size: 6508 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3554 3835 1872 Size: 1682 281 4545 Fragments arranged by size: 4545 1682 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 4612 5513 5775 820 Size: 197 926 2669 901 262 1553 Fragments arranged by size: 2669 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3805 4299 493 Size: 3312 494 2702 Fragments arranged by size: 3312 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 4432 5 Size: 47 4380 2081 Fragments arranged by size: 4380 2081 47 StuI AGG'CCT Cuts at: 1517 2366 3275 1517 Size: 849 909 4750 Fragments arranged by size: 4750 909 849 StyI C'CwwG_G Cuts at: 513 1065 2022 2067 2091 2176 2377 3435 3513 Size: 552 957 45 24 85 201 1058 78 Cuts at: 3513 3947 513 Size: 434 3074 Fragments arranged by size: 3074 1058 957 552 434 201 85 78 45 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2690 3353 4535 4552 4705 4890 5335 Size: 1255 1110 663 1182 17 153 185 445 Cuts at: 5335 325 Size: 1498 Fragments arranged by size: 1498 1255 1182 1110 663 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3381 4754 6466 Size: 274 80 33 51 1443 1404 1373 1712 Cuts at: 6466 96 Size: 138 Fragments arranged by size: 1712 1443 1404 1373 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2224 2256 2373 2405 2683 2715 2948 2980 6468 Size: 32 117 32 278 32 233 32 3488 Cuts at: 6468 6500 2224 Size: 32 2232 Fragments arranged by size: 3488 2232 278 233 117 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3578 1316 Size: 2262 4246 XbaI T'CTAG_A Cuts at: 3844 3844 Size: 6508 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2810 3520 2810 Size: 710 5798 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6508 XmaI C'CCGG_G Cuts at: 1870 3552 3833 1870 Size: 1682 281 4545 Fragments arranged by size: 4545 1682 281 XmnI GAAnn'nnTTC Cuts at: 2597 4637 2597 Size: 2040 4468 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI