Restriction map for
pFN21ASDA1599
(Linear) (Six-base) MAPSORT of: KIAA1599.seq Check: 3306 from: 1 to: 1779
KIAA1599.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 148 1779
Size: 148 1631
AclI AA'CG_TT
Cuts at: 0 631 1779
Size: 631 1148
AflIII A'CryG_T
Cuts at: 0 113 1779
Size: 113 1666
AhdI GACnn_n'nnGTC
Cuts at: 0 21 1779
Size: 21 1758
AleI CACnn'nnGTG
Cuts at: 0 70 1318 1779
Size: 70 1248 461
Fragments arranged by size:
1248 461 70
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 566 1621 1779
Size: 460 106 1055 158
Fragments arranged by size:
1055 460 158 106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 616 648 1779
Size: 616 32 1131
Fragments arranged by size:
1131 616 32
AlwNI CAG_nnn'CTG
Cuts at: 0 521 1779
Size: 521 1258
ApaLI G'TGCA_C
Cuts at: 0 942 1779
Size: 942 837
ApoI r'AATT_y
Cuts at: 0 423 897 1779
Size: 423 474 882
Fragments arranged by size:
882 474 423
AvaI C'yCGr_G
Cuts at: 0 1529 1628 1779
Size: 1529 99 151
Fragments arranged by size:
1529 151 99
BanI G'GyrC_C
Cuts at: 0 487 1779
Size: 487 1292
BanII G_rGCy'C
Cuts at: 0 515 714 1057 1779
Size: 515 199 343 722
Fragments arranged by size:
722 515 343 199
BbvCI CC'TCA_GC
Cuts at: 0 506 1779
Size: 506 1273
BglI GCCn_nnn'nGGC
Cuts at: 0 1624 1779
Size: 1624 155
BlpI GC'TnA_GC
Cuts at: 0 28 514 1609 1779
Size: 28 486 1095 170
Fragments arranged by size:
1095 486 170 28
Bme1580I G_kGCm'C
Cuts at: 0 946 1779
Size: 946 833
BmgBI CAC'GTC
Cuts at: 0 1515 1635 1779
Size: 1515 120 144
Fragments arranged by size:
1515 144 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 771 1350 1779
Size: 771 579 429
Fragments arranged by size:
771 579 429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 1236 1779
Size: 57 1179 543
Fragments arranged by size:
1179 543 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1779
Size: 1267 512
Bpu10I CC'TnA_GC
Cuts at: 0 506 1779
Size: 506 1273
BsaAI yAC'GTr
Cuts at: 0 894 1320 1584 1605 1779
Size: 894 426 264 21 174
Fragments arranged by size:
894 426 264 174 21
BsaHI Gr'CG_yC
Cuts at: 0 22 1487 1779
Size: 22 1465 292
Fragments arranged by size:
1465 292 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 350 380 925 955 1779
Size: 350 30 545 30 824
Fragments arranged by size:
824 545 350 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 523 1515 1779
Size: 274 249 992 264
Fragments arranged by size:
992 274 264 249
BseYI C'CCAG_C
Cuts at: 0 1737 1779
Size: 1737 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 1027 1297 1363 1750 1779
Size: 333 694 270 66 387 29
Fragments arranged by size:
694 387 333 270 66 29
BsiEI CG_ry'CG
Cuts at: 0 735 1340 1779
Size: 735 605 439
Fragments arranged by size:
735 605 439
BsiHKAI G_wGCw'C
Cuts at: 0 515 946 1779
Size: 515 431 833
Fragments arranged by size:
833 515 431
BsmI GAATG_Cn'
Cuts at: 0 412 1335 1685 1779
Size: 412 923 350 94
Fragments arranged by size:
923 412 350 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1625 1779
Size: 1625 154
Bsp1286I G_dGCh'C
Cuts at: 0 515 714 946 1057 1779
Size: 515 199 232 111 722
Fragments arranged by size:
722 515 232 199 111
BspHI T'CATG_A
Cuts at: 0 659 1779
Size: 659 1120
BssSI C'ACGA_G
Cuts at: 0 1186 1779
Size: 1186 593
BstEII G'GTnAC_C
Cuts at: 0 1311 1779
Size: 1311 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 561 747 1779
Size: 72 489 186 1032
Fragments arranged by size:
1032 489 186 72
BstYI r'GATC_y
Cuts at: 0 1520 1646 1779
Size: 1520 126 133
Fragments arranged by size:
1520 133 126
BstZ17I GTA'TAC
Cuts at: 0 149 1779
Size: 149 1630
Bsu36I CC'TnA_GG
Cuts at: 0 11 1779
Size: 11 1768
BtgI C'CryG_G
Cuts at: 0 825 1307 1490 1779
Size: 825 482 183 289
Fragments arranged by size:
825 482 289 183
BtsI GCAGTG_nn'
Cuts at: 0 178 1004 1779
Size: 178 826 775
Fragments arranged by size:
826 775 178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1281 1316 1779
Size: 1281 35 463
Fragments arranged by size:
1281 463 35
EaeI y'GGCC_r
Cuts at: 0 62 992 1322 1337 1779
Size: 62 930 330 15 442
Fragments arranged by size:
930 442 330 62 15
EagI C'GGCC_G
Cuts at: 0 1337 1779
Size: 1337 442
EcoICRI GAG'CTC
Cuts at: 0 513 1779
Size: 513 1266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1779
Size: 1267 512
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 1779
Size: 337 1442
EcoO109I rG'GnC_Cy
Cuts at: 0 371 971 1779
Size: 371 600 808
Fragments arranged by size:
808 600 371
EcoRI G'AATT_C
Cuts at: 0 423 897 1779
Size: 423 474 882
Fragments arranged by size:
882 474 423
FspI TGC'GCA
Cuts at: 0 232 1270 1779
Size: 232 1038 509
Fragments arranged by size:
1038 509 232
HaeII r_GCGC'y
Cuts at: 0 1087 1779
Size: 1087 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 283 315 1091 1123 1779
Size: 7 32 244 32 776 32 656
Fragments arranged by size:
776 656 244 32 32 32 7
HincII GTy'rAC
Cuts at: 0 297 1428 1779
Size: 297 1131 351
Fragments arranged by size:
1131 351 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 482 1077 1779
Size: 152 330 595 702
Fragments arranged by size:
702 595 330 152
MscI TGG'CCA
Cuts at: 0 994 1324 1779
Size: 994 330 455
Fragments arranged by size:
994 455 330
MslI CAynn'nnrTG
Cuts at: 0 70 703 745 1318 1779
Size: 70 633 42 573 461
Fragments arranged by size:
633 573 461 70 42
MspA1I CmG'CkG
Cuts at: 0 451 505 1065 1281 1336 1779
Size: 451 54 560 216 55 443
Fragments arranged by size:
560 451 443 216 55 54
NcoI C'CATG_G
Cuts at: 0 1490 1779
Size: 1490 289
NspI r_CATG'y
Cuts at: 0 117 1779
Size: 117 1662
PasI CC'CwG_GG
Cuts at: 0 376 1143 1779
Size: 376 767 636
Fragments arranged by size:
767 636 376
PciI A'CATG_T
Cuts at: 0 113 1779
Size: 113 1666
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 1201 1779
Size: 159 1042 578
Fragments arranged by size:
1042 578 159
PfoI T'CCnGG_A
Cuts at: 0 101 427 1244 1574 1779
Size: 101 326 817 330 205
Fragments arranged by size:
817 330 326 205 101
PmlI CAC'GTG
Cuts at: 0 1320 1605 1779
Size: 1320 285 174
Fragments arranged by size:
1320 285 174
PpuMI rG'GwC_Cy
Cuts at: 0 371 971 1779
Size: 371 600 808
Fragments arranged by size:
808 600 371
PshAI GACnn'nnGTC
Cuts at: 0 655 1093 1779
Size: 655 438 686
Fragments arranged by size:
686 655 438
PstI C_TGCA'G
Cuts at: 0 94 1779
Size: 94 1685
PvuI CG_AT'CG
Cuts at: 0 735 1779
Size: 735 1044
PvuII CAG'CTG
Cuts at: 0 451 1065 1281 1779
Size: 451 614 216 498
Fragments arranged by size:
614 498 451 216
SacI G_AGCT'C
Cuts at: 0 515 1779
Size: 515 1264
SanDI GG'GwC_CC
Cuts at: 0 371 971 1779
Size: 371 600 808
Fragments arranged by size:
808 600 371
SbfI CC_TGCA'GG
Cuts at: 0 94 1779
Size: 94 1685
ScaI AGT'ACT
Cuts at: 0 1360 1779
Size: 1360 419
SfcI C'TryA_G
Cuts at: 0 90 522 609 1779
Size: 90 432 87 1170
Fragments arranged by size:
1170 432 90 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1624 1779
Size: 1624 155
SmaI CCC'GGG
Cuts at: 0 1531 1779
Size: 1531 248
StuI AGG'CCT
Cuts at: 0 343 1252 1779
Size: 343 909 527
Fragments arranged by size:
909 527 343
StyI C'CwwG_G
Cuts at: 0 44 68 153 354 1412 1490 1779
Size: 44 24 85 201 1058 78 289
Fragments arranged by size:
1058 289 201 85 78 44 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 1330 1779
Size: 667 663 449
Fragments arranged by size:
667 663 449
TatI w'GTAC_w
Cuts at: 0 1358 1779
Size: 1358 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 350 382 660 692 925 957
Size: 201 32 117 32 278 32 233 32
Cuts at: 957 1779
Size: 822
Fragments arranged by size:
822 278 233 201 117 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1555 1779
Size: 1555 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 787 1497 1779
Size: 787 710 282
Fragments arranged by size:
787 710 282
XmaI C'CCGG_G
Cuts at: 0 1529 1779
Size: 1529 250
XmnI GAAnn'nnTTC
Cuts at: 0 574 1779
Size: 574 1205
Enzymes that do cut:
AccI AclI AflIII AhdI AleI AlfI AloI AlwNI
ApaLI ApoI AvaI BanI BanII BbvCI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BssSI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI CspCI EaeI EagI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PmlI PpuMI PshAI PstI PvuI PvuII SacI SanDI
SbfI ScaI SfcI SfiI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AgeI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BciVI BclI BfrBI BglII BmtI BpuEI BsaI
BsaBI BsaWI BsiWI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BtgZI ClaI DraI DraIII
DrdI EarI EciI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI
PsiI PspOMI PspXI PsrI RsrII SacII SalI SapI
SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI ZraI