Restriction map for
pFN21ASDA1589
##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AclI AflIII AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BglII BlnI BsaAI
BsiEI BsiWI BsmBI BsmI BspLU11I
BssHII BstBI BstXI Bsu36I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1824 GACGTC GACGT^C
AccII 2115 CGCG CG^CG
AccIII 1031 TCCGGA T^CCGGA
AcyI 1824 GACGTC G[GA]^CG[CT]C
AflII 760 CTTAAG C^TTAAG
AgeI 1025 ACCGGT A^CCGGT
Alw44I 964 GTGCAC G^TGCAC
ApaLI 964 GTGCAC G^TGCAC
AspI 781 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1176 CCCGGG C^[CT]CG[GA]G
BbsI 496 GAAGAC GAAGAC(2/6)
BbvCI 1299 CCTCAGC CCTCAGC(-5/-2)
BciVI 1593 GGATAC GTATCC(6/5)
BfrI 760 CTTAAG C^TTAAG
BglI 1195 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1719 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1719 GCTTAGC GC^T[ACGT]AGC
BpuAI 496 GAAGAC GAAGAC(2/6)
BsaBI 529 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1824 GACGTC G[GA]^CG[CT]C
BseAI 1031 TCCGGA T^CCGGA
BsoBI 1176 CCCGGG C^[CT]CG[GA]G
Bsp1407I 359 TGTACA T^GTACA
BspDI 700 ATCGAT AT^CGAT
BspEI 1031 TCCGGA T^CCGGA
BspHI 1356 TCATGA T^CATGA
BsrDI 2028 CATTGC GCAATG(2/0)
BsrGI 359 TGTACA T^GTACA
BssSI 2270 CTCGTG CACGAG(-5/-1)
Bst1107I 1407 GTATAC GTA^TAC
BstEII 2323 GGTGACC G^GT[ACGT]ACC
BstPI 2323 GGTGACC G^GT[ACGT]ACC
BstUI 2115 CGCG CG^CG
BstZ17I 1407 GTATAC GTA^TAC
CelII 1719 GCTTAGC GC^T[ACGT]AGC
ClaI 700 ATCGAT AT^CGAT
DraIII 1205 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2323 GGTGACC G^GT[ACGT]ACC
HgaI 2113 GACGC GACGC(5/10)
Hin1I 1824 GACGTC G[GA]^CG[CT]C
HindIII 854 AAGCTT A^AGCTT
MamI 529 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1031 TCCGGA T^CCGGA
MslI 1387 CACCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2115 CGCG CG^CG
NspI 1620 GCATGT [GA]CATG^[CT]
PflFI 781 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 972 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1025 ACCGGT A^CCGGT
PpuMI 764 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1356 TCATGA T^CATGA
ScaI 1565 AGTACT AGT^ACT
SexAI 243 ACCAGGT A^CC[AT]GGT
SmaI 1176 CCCGGG CCC^GGG
SspBI 359 TGTACA T^GTACA
Tth111I 781 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 972 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1176 CCCGGG C^CCGGG
XmaI 1176 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 460 GTAGAC GT^[AC][GT]AC
1407 GTATAC
AcsI 476 AAATTC [GA]^AATT[CT]
832 AAATTC
AlwI 1230 GATCC GGATC(4/5)
1585 GATCC
ApaI 933 GGGCCC GGGCC^C
1929 GGGCCC
ApoI 476 AAATTC [GA]^AATT[CT]
832 AAATTC
BalI 195 TGGCCA TGG^CCA
770 TGGCCA
BclI 528 TGATCA T^GATCA
987 TGATCA
BsaWI 1025 ACCGGT [AT]^CCGG[AT]
1031 TCCGGA
BsgI 648 CTGCAC GTGCAG(16/14)
2053 GTGCAG
BsrBI 175 GAGCGG CCGCTC(-3/-3)
1918 GAGCGG
BsrFI 809 ACCGGC [GA]^CCGG[CT]
1025 ACCGGT
BstAPI 1415 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1498 GCAAAATATGC
BstYI 1229 AGATCC [GA]^GATC[CT]
1584 AGATCC
Cfr10I 809 ACCGGC [GA]^CCGG[CT]
1025 ACCGGT
DraI 622 TTTAAA TTT^AAA
1831 TTTAAA
DrdI 636 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2281 GACCATCCCGTC
DsaI 1103 CCACGG C^C[GA][CT]GG
2072 CCGTGG
EcoNI 216 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1778 CCTACTGGAGG
FbaI 528 TGATCA T^GATCA
987 TGATCA
HaeII 158 AGCGCC [GA]GCGC^[CT]
1673 AGCGCC
MaeII 1825 ACGT A^CGT
1976 ACGT
MflI 1229 AGATCC [GA]^GATC[CT]
1584 AGATCC
MluNI 195 TGGCCA TGG^CCA
770 TGGCCA
MscI 195 TGGCCA TGG^CCA
770 TGGCCA
PvuII 1302 CAGCTG CAG^CTG
1887 CAGCTG
SacI 142 GAGCTC GAGCT^C
880 GAGCTC
SmlI 760 CTTAAG C^T[CT][GA]AG
883 CTCAAG
StuI 1418 AGGCCT AGG^CCT
2122 AGGCCT
XhoII 1229 AGATCC [GA]^GATC[CT]
1584 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI