(Circular) (Six-base) MAPSORT of: pFN21ASDA1589.seq Check: 5421 from: 1 to: 7060 pFN21ASDA1589 7060 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3314 3314 Size: 7060 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3851 4870 278 Size: 53 83 186 936 2315 1019 2468 Fragments arranged by size: 2468 2315 1019 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2484 3431 4403 1937 Size: 547 947 972 4594 Fragments arranged by size: 4594 972 947 547 Acc65I G'GTAC_C Cuts at: 4381 4381 Size: 7060 AclI AA'CG_TT Cuts at: 1563 5187 5560 1563 Size: 3624 373 3063 Fragments arranged by size: 3624 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 3096 3226 3242 3692 5123 6507 6775 Size: 1274 190 130 16 450 1431 1384 268 Cuts at: 6775 1632 Size: 1917 Fragments arranged by size: 1917 1431 1384 1274 450 268 190 130 16 AflII C'TTAA_G Cuts at: 820 1017 2783 820 Size: 197 1766 5097 Fragments arranged by size: 5097 1766 197 AflIII A'CryG_T Cuts at: 1203 5959 6936 1203 Size: 4756 977 1327 Fragments arranged by size: 4756 1327 977 AgeI A'CCGG_T Cuts at: 3048 5891 3048 Size: 2843 4217 AhdI GACnn_n'nnGTC Cuts at: 5789 5789 Size: 7060 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7060 AlwNI CAG_nnn'CTG Cuts at: 2560 3001 3700 3826 3938 4803 6375 2560 Size: 441 699 126 112 865 1572 3245 Fragments arranged by size: 3245 1572 865 699 441 126 112 ApaI G_GGCC'C Cuts at: 2960 3956 2960 Size: 996 6064 ApaLI G'TGCA_C Cuts at: 2987 5117 6273 2987 Size: 2130 1156 3774 Fragments arranged by size: 3774 2130 1156 ApoI r'AATT_y Cuts at: 1493 2499 2855 4369 4595 4747 6856 1493 Size: 1006 356 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 1006 356 226 152 AseI AT'TA_AT Cuts at: 160 1032 5614 160 Size: 872 4582 1606 Fragments arranged by size: 4582 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 AvaI C'yCGr_G Cuts at: 1870 1943 3199 4385 1870 Size: 73 1256 1186 4545 Fragments arranged by size: 4545 1256 1186 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3609 3642 3609 Size: 33 7027 BamHI G'GATC_C Cuts at: 4390 4757 4390 Size: 367 6693 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 3025 3195 4159 4381 Size: 325 217 627 508 730 170 964 222 Cuts at: 4381 5836 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 964 730 627 508 325 222 217 170 BanII G_rGCy'C Cuts at: 1391 2169 2739 2907 2960 3008 3956 4379 1391 Size: 778 570 168 53 48 948 423 4072 Fragments arranged by size: 4072 948 778 570 423 168 53 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2526 928 Size: 971 627 5462 Fragments arranged by size: 5462 971 627 BbvCI CC'TCA_GC Cuts at: 3323 3323 Size: 7060 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5251 5285 1903 Size: 34 3314 34 3678 Fragments arranged by size: 3678 3314 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3610 4952 6162 3610 Size: 1342 1210 4508 Fragments arranged by size: 4508 1342 1210 BclI T'GATC_A Cuts at: 1548 2551 3010 1548 Size: 1003 459 5598 Fragments arranged by size: 5598 1003 459 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3224 5671 136 Size: 107 122 71 631 605 1552 2447 1525 Fragments arranged by size: 2447 1552 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7055 7055 Size: 7060 BlpI GC'TnA_GC Cuts at: 3743 4477 3743 Size: 734 6326 Bme1580I G_kGCm'C Cuts at: 1790 2032 2960 2991 3480 3956 5121 6277 1790 Size: 242 928 31 489 476 1165 1156 2573 Fragments arranged by size: 2573 1165 1156 928 489 476 242 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2696 2963 3011 Size: 416 53 83 399 24 1279 267 48 Cuts at: 3011 4165 4791 5749 442 Size: 1154 626 958 1753 Fragments arranged by size: 1753 1279 1154 958 626 416 399 267 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2384 2432 3826 5720 1239 Size: 1106 39 48 1394 1894 2579 Fragments arranged by size: 2579 1894 1394 1106 48 39 Bpu10I CC'TnA_GC Cuts at: 3323 3939 3323 Size: 616 6444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2891 5185 6050 6348 2891 Size: 2294 865 298 3603 Fragments arranged by size: 3603 2294 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 2429 2975 3521 5723 882 Size: 617 756 174 546 546 2202 2219 Fragments arranged by size: 2219 2202 756 617 546 546 174 BsaAI yAC'GTr Cuts at: 493 1204 4357 4851 493 Size: 711 3153 494 2702 Fragments arranged by size: 3153 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2556 7054 2556 Size: 4498 2562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3848 4867 5249 5884 Size: 53 83 186 936 2315 1019 382 635 Cuts at: 5884 275 Size: 1451 Fragments arranged by size: 2315 1451 1019 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3048 3054 5493 5891 6165 6312 6723 3048 Size: 6 2439 398 274 147 411 3385 Fragments arranged by size: 3385 2439 411 398 274 147 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3172 3202 4083 4113 1178 Size: 30 165 30 1769 30 881 30 4125 Fragments arranged by size: 4125 1769 881 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2177 2447 3301 1186 Size: 988 3 270 854 4945 Fragments arranged by size: 4945 988 854 270 3 BseYI C'CCAG_C Cuts at: 2041 2994 3061 3693 3948 4299 6263 6759 2041 Size: 953 67 632 255 351 1964 496 2342 Fragments arranged by size: 2342 1964 953 632 496 351 255 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2656 4097 1163 Size: 573 920 1441 4126 Fragments arranged by size: 4126 1441 920 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4510 5271 6299 1540 Size: 275 186 2509 761 1028 2301 Fragments arranged by size: 2509 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2907 2991 4268 5121 5206 6277 2169 Size: 738 84 1277 853 85 1071 2952 Fragments arranged by size: 2952 1277 1071 853 738 85 84 BsmI GAATG_Cn' Cuts at: 4571 4664 4571 Size: 93 6967 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2169 2739 2907 2960 2991 3008 Size: 399 242 137 570 168 53 31 17 Cuts at: 3008 3480 3956 4268 4379 5121 5206 6277 1391 Size: 472 476 312 111 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 476 472 399 312 242 168 137 111 85 53 31 17 BspEI T'CCGG_A Cuts at: 3054 6723 3054 Size: 3669 3391 BspHI T'CATG_A Cuts at: 3379 4949 6679 3379 Size: 1570 1730 3760 Fragments arranged by size: 3760 1730 1570 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2312 3314 3443 4415 844 Size: 1468 1002 129 972 3489 Fragments arranged by size: 3489 1468 1002 972 129 BsrBI CCG'CTC Cuts at: 1394 1823 2200 3943 4947 1394 Size: 429 377 1743 1004 3507 Fragments arranged by size: 3507 1743 1004 429 377 BsrDI GCAATG_nn' Cuts at: 65 1248 4050 5555 5729 65 Size: 1183 2802 1505 174 1396 Fragments arranged by size: 2802 1505 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2832 3048 5704 5891 1700 Size: 110 1022 216 2656 187 2869 Fragments arranged by size: 2869 2656 1022 216 187 110 BsrGI T'GTAC_A Cuts at: 96 2382 96 Size: 2286 4774 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7060 BssSI C'ACGA_G Cuts at: 4293 5120 6132 4293 Size: 827 1012 5221 Fragments arranged by size: 5221 1012 827 BstAPI GCAn_nnn'nTGC Cuts at: 794 3444 3527 794 Size: 2650 83 4327 Fragments arranged by size: 4327 2650 83 BstBI TT'CG_AA Cuts at: 2007 5864 2007 Size: 3857 3203 BstEII G'GTnAC_C Cuts at: 1184 4346 1184 Size: 3162 3898 BstYI r'GATC_y Cuts at: 1972 3252 3607 4390 4757 5142 5159 6611 6697 Size: 1280 355 783 367 385 17 1452 86 Cuts at: 6697 6709 7055 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1280 783 385 367 355 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3432 3432 Size: 7060 BtgI C'CryG_G Cuts at: 513 1065 3126 4095 513 Size: 552 2061 969 3478 Fragments arranged by size: 3478 2061 969 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6321 Fragments arranged by size: 6321 683 56 BtsI GCAGTG_nn' Cuts at: 2150 2724 2916 3877 4180 4228 4576 5370 5390 Size: 574 192 961 303 48 348 794 20 Cuts at: 5390 2150 Size: 3820 Fragments arranged by size: 3820 961 794 574 348 303 192 48 20 ClaI AT'CG_AT Cuts at: 1554 2724 1554 Size: 1170 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7025 DraI TTT'AAA Cuts at: 2647 3856 4364 2647 Size: 1209 508 5343 Fragments arranged by size: 5343 1209 508 DraIII CAC_nnn'GTG Cuts at: 3233 3233 Size: 7060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2665 4310 6067 809 Size: 507 1349 1645 1757 1802 Fragments arranged by size: 1802 1757 1645 1349 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 2793 3929 Size: 54 1425 50 275 18 388 575 1136 Cuts at: 3929 4140 4507 5396 7025 8 Size: 211 367 889 1629 43 Fragments arranged by size: 1629 1425 1136 889 575 388 367 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4507 1537 Size: 275 2695 4090 Fragments arranged by size: 4090 2695 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2415 2855 4990 1353 Size: 977 85 440 2135 3423 Fragments arranged by size: 3423 2135 977 440 85 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5647 6021 6167 387 Size: 1064 138 4058 374 146 1280 Fragments arranged by size: 4058 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2167 2905 2167 Size: 738 6322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2384 2432 2906 3096 3226 3242 Size: 393 713 39 48 474 190 130 16 Cuts at: 3242 3692 3826 5123 5720 6507 6775 1239 Size: 450 134 1297 597 787 268 1524 Fragments arranged by size: 1524 1297 787 713 597 474 450 393 268 190 134 130 48 39 16 EcoNI CCTnn'n_nnAGG Cuts at: 2243 3805 2243 Size: 1562 5498 EcoO109I rG'GnC_Cy Cuts at: 2053 2788 2957 3952 3953 2053 Size: 735 169 995 1 5160 Fragments arranged by size: 5160 995 735 169 1 EcoRI G'AATT_C Cuts at: 4369 4747 4369 Size: 378 6682 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2106 2106 Size: 7060 FspI TGC'GCA Cuts at: 5566 5566 Size: 7060 HaeII r_GCGC'y Cuts at: 1678 2185 3700 6207 1678 Size: 507 1515 2507 2531 Fragments arranged by size: 2531 2507 1515 507 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 3002 3034 3538 Size: 32 135 32 1013 32 385 32 504 Cuts at: 3538 3570 4250 4282 5701 5733 5775 5807 1373 Size: 32 680 32 1419 32 42 32 2626 Fragments arranged by size: 2626 1419 1013 680 504 385 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4404 4650 669 Size: 988 281 2466 246 3079 Fragments arranged by size: 3079 2466 988 281 246 HindIII A'AGCT_T Cuts at: 748 2877 748 Size: 2129 4931 HpaI GTT'AAC Cuts at: 4650 4650 Size: 7060 KpnI G_GTAC'C Cuts at: 4385 4385 Size: 7060 MfeI C'AATT_G Cuts at: 4659 4659 Size: 7060 MluI A'CGCG_T Cuts at: 6936 6936 Size: 7060 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3316 3684 6174 6358 1305 Size: 2011 368 2490 184 2007 Fragments arranged by size: 2490 2011 2007 368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2220 2795 10 Size: 54 1425 343 388 575 4275 Fragments arranged by size: 4275 1425 575 388 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3414 5018 5377 5536 518 Size: 648 2248 1604 359 159 2042 Fragments arranged by size: 2248 2042 1604 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2154 3246 3327 3396 3912 4476 5153 Size: 491 57 1092 81 69 516 564 677 Cuts at: 5153 6301 6546 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 564 516 491 245 81 69 57 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6508 NdeI CA'TA_TG Cuts at: 387 387 Size: 7060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7060 NotI GC'GGCC_GC Cuts at: 4507 4507 Size: 7060 NspI r_CATG'y Cuts at: 1132 1229 3647 4418 5963 1132 Size: 97 2418 771 1545 2229 Fragments arranged by size: 2418 2229 1545 771 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7060 PciI A'CATG_T Cuts at: 5959 5959 Size: 7060 PflMI CCAn_nnn'nTGG Cuts at: 1108 3001 1108 Size: 1893 5167 PfoI T'CCnGG_A Cuts at: 2400 3042 2400 Size: 642 6418 PmeI GTTT'AAAC Cuts at: 4364 4364 Size: 7060 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5129 5161 6642 6674 5129 Size: 32 1481 32 5515 Fragments arranged by size: 5515 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 2788 Size: 7060 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7060 PsiI TTA'TAA Cuts at: 4630 4630 Size: 7060 PspOMI G'GGCC_C Cuts at: 2956 3952 2956 Size: 996 6064 PstI C_TGCA'G Cuts at: 830 2138 2145 2965 3401 4412 830 Size: 1308 7 820 436 1011 3478 Fragments arranged by size: 3478 1308 1011 820 436 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7060 PvuII CAG'CTG Cuts at: 3327 3912 3327 Size: 585 6475 SacI G_AGCT'C Cuts at: 2169 2907 2169 Size: 738 6322 SalI G'TCGA_C Cuts at: 1936 4402 1936 Size: 2466 4594 SbfI CC_TGCA'GG Cuts at: 4412 4412 Size: 7060 ScaI AGT'ACT Cuts at: 3590 5308 7020 3590 Size: 1718 1712 3630 Fragments arranged by size: 3630 1718 1712 SexAI A'CCwGG_T Cuts at: 2266 2266 Size: 7060 SfcI C'TryA_G Cuts at: 826 1045 2134 2141 2961 3397 4408 5543 6224 Size: 219 1089 7 820 436 1011 1135 681 Cuts at: 6224 6415 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1089 1011 820 681 436 219 191 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7060 SmaI CCC'GGG Cuts at: 1872 3201 4387 1872 Size: 1329 1186 4545 Fragments arranged by size: 4545 1329 1186 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 2906 5164 6065 6327 820 Size: 197 926 840 123 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 840 262 197 123 SnaBI TAC'GTA Cuts at: 493 4357 4851 493 Size: 3864 494 2702 Fragments arranged by size: 3864 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7060 SphI G_CATG'C Cuts at: 1132 4418 1132 Size: 3286 3774 SspI AAT'ATT Cuts at: 5 52 4984 5 Size: 47 4932 2081 Fragments arranged by size: 4932 2081 47 StuI AGG'CCT Cuts at: 1517 3443 4147 1517 Size: 1926 704 4430 Fragments arranged by size: 4430 1926 704 StyI C'CwwG_G Cuts at: 513 1065 2860 2898 3748 3759 4499 513 Size: 552 1795 38 850 11 740 3074 Fragments arranged by size: 3074 1795 850 740 552 38 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3008 4147 5087 5104 5257 5442 5887 Size: 1255 1428 1139 940 17 153 185 445 Cuts at: 5887 325 Size: 1498 Fragments arranged by size: 1498 1428 1255 1139 940 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 3588 5306 Size: 274 80 33 51 1443 405 1206 1718 Cuts at: 5306 7018 96 Size: 1712 138 Fragments arranged by size: 1718 1712 1443 1206 405 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3172 3204 4083 4115 7020 7052 3172 Size: 32 879 32 2905 32 3180 Fragments arranged by size: 3180 2905 879 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2807 1316 Size: 1491 5569 XbaI T'CTAG_A Cuts at: 4396 4396 Size: 7060 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7060 XmaI C'CCGG_G Cuts at: 1870 3199 4385 1870 Size: 1329 1186 4545 Fragments arranged by size: 4545 1329 1186 XmnI GAAnn'nnTTC Cuts at: 2499 2891 4202 5189 2499 Size: 392 1311 987 4370 Fragments arranged by size: 4370 1311 987 392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3849 4868 276 Size: 53 83 186 936 2315 1019 2468 Fragments arranged by size: 2468 2315 1019 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI