Restriction map for
pFN21ASDA1589
(Linear) (Six-base) MAPSORT of: KIAA1589.seq Check: 9309 from: 1 to: 2331
KIAA1589.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1291 2331
Size: 1291 1040
AatII G_ACGT'C
Cuts at: 0 1828 2331
Size: 1828 503
AccI GT'mk_AC
Cuts at: 0 461 1408 2331
Size: 461 947 923
Fragments arranged by size:
947 923 461
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1073 1203 1219 1669 2331
Size: 883 190 130 16 450 662
Fragments arranged by size:
883 662 450 190 130 16
AflII C'TTAA_G
Cuts at: 0 760 2331
Size: 760 1571
AgeI A'CCGG_T
Cuts at: 0 1025 2331
Size: 1025 1306
AlwNI CAG_nnn'CTG
Cuts at: 0 537 978 1677 1803 1915 2331
Size: 537 441 699 126 112 416
Fragments arranged by size:
699 537 441 416 126 112
ApaI G_GGCC'C
Cuts at: 0 937 1933 2331
Size: 937 996 398
Fragments arranged by size:
996 937 398
ApaLI G'TGCA_C
Cuts at: 0 964 2331
Size: 964 1367
ApoI r'AATT_y
Cuts at: 0 476 832 2331
Size: 476 356 1499
Fragments arranged by size:
1499 476 356
AvaI C'yCGr_G
Cuts at: 0 1176 2331
Size: 1176 1155
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1586 1619 2331
Size: 1586 33 712
Fragments arranged by size:
1586 712 33
BanI G'GyrC_C
Cuts at: 0 272 1002 1172 2136 2331
Size: 272 730 170 964 195
Fragments arranged by size:
964 730 272 195 170
BanII G_rGCy'C
Cuts at: 0 146 716 884 937 985 1933 2331
Size: 146 570 168 53 48 948 398
Fragments arranged by size:
948 570 398 168 146 53 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 2331
Size: 503 1828
BbvCI CC'TCA_GC
Cuts at: 0 1300 2331
Size: 1300 1031
BciVI GTATCCnnnnn_n'
Cuts at: 0 1587 2331
Size: 1587 744
BclI T'GATC_A
Cuts at: 0 528 987 2331
Size: 528 459 1344
Fragments arranged by size:
1344 528 459
BglI GCCn_nnn'nGGC
Cuts at: 0 1201 2331
Size: 1201 1130
BlpI GC'TnA_GC
Cuts at: 0 1720 2331
Size: 1720 611
Bme1580I G_kGCm'C
Cuts at: 0 9 937 968 1457 1933 2331
Size: 9 928 31 489 476 398
Fragments arranged by size:
928 489 476 398 31 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 673 940 988 2142 2331
Size: 673 267 48 1154 189
Fragments arranged by size:
1154 673 267 189 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 361 409 1803 2331
Size: 322 39 48 1394 528
Fragments arranged by size:
1394 528 322 48 39
Bpu10I CC'TnA_GC
Cuts at: 0 1300 1916 2331
Size: 1300 616 415
Fragments arranged by size:
1300 616 415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 2331
Size: 868 1463
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 406 952 1498 2331
Size: 232 174 546 546 833
Fragments arranged by size:
833 546 546 232 174
BsaBI GATnn'nnATC
Cuts at: 0 533 2331
Size: 533 1798
BsaHI Gr'CG_yC
Cuts at: 0 1825 2331
Size: 1825 506
BsaWI w'CCGG_w
Cuts at: 0 1025 1031 2331
Size: 1025 6 1300
Fragments arranged by size:
1300 1025 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1149 1179 2060 2090 2331
Size: 1149 30 881 30 241
Fragments arranged by size:
1149 881 241 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 154 424 1278 2331
Size: 151 3 270 854 1053
Fragments arranged by size:
1053 854 270 151 3
BseYI C'CCAG_C
Cuts at: 0 18 971 1038 1670 1925 2276 2331
Size: 18 953 67 632 255 351 55
Fragments arranged by size:
953 632 351 255 67 55 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 2074 2331
Size: 633 1441 257
Fragments arranged by size:
1441 633 257
BsiHKAI G_wGCw'C
Cuts at: 0 146 884 968 2245 2331
Size: 146 738 84 1277 86
Fragments arranged by size:
1277 738 146 86 84
Bsp1286I G_dGCh'C
Cuts at: 0 9 146 716 884 937 968 985 1457
Size: 9 137 570 168 53 31 17 472
Cuts at: 1457 1933 2245 2331
Size: 476 312 86
Fragments arranged by size:
570 476 472 312 168 137 86 53
31 17 9
BspEI T'CCGG_A
Cuts at: 0 1031 2331
Size: 1031 1300
BspHI T'CATG_A
Cuts at: 0 1356 2331
Size: 1356 975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 1291 1420 2331
Size: 289 1002 129 911
Fragments arranged by size:
1002 911 289 129
BsrBI CCG'CTC
Cuts at: 0 177 1920 2331
Size: 177 1743 411
Fragments arranged by size:
1743 411 177
BsrDI GCAATG_nn'
Cuts at: 0 2027 2331
Size: 2027 304
BsrFI r'CCGG_y
Cuts at: 0 809 1025 2331
Size: 809 216 1306
Fragments arranged by size:
1306 809 216
BsrGI T'GTAC_A
Cuts at: 0 359 2331
Size: 359 1972
BssSI C'ACGA_G
Cuts at: 0 2270 2331
Size: 2270 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1421 1504 2331
Size: 1421 83 827
Fragments arranged by size:
1421 827 83
BstEII G'GTnAC_C
Cuts at: 0 2323 2331
Size: 2323 8
BstYI r'GATC_y
Cuts at: 0 1229 1584 2331
Size: 1229 355 747
Fragments arranged by size:
1229 747 355
BstZ17I GTA'TAC
Cuts at: 0 1409 2331
Size: 1409 922
BtgI C'CryG_G
Cuts at: 0 1103 2072 2331
Size: 1103 969 259
Fragments arranged by size:
1103 969 259
BtsI GCAGTG_nn'
Cuts at: 0 127 701 893 1854 2157 2205 2331
Size: 127 574 192 961 303 48 126
Fragments arranged by size:
961 574 303 192 127 126 48
ClaI AT'CG_AT
Cuts at: 0 701 2331
Size: 701 1630
DraI TTT'AAA
Cuts at: 0 624 1833 2331
Size: 624 1209 498
Fragments arranged by size:
1209 624 498
DraIII CAC_nnn'GTG
Cuts at: 0 1210 2331
Size: 1210 1121
DrdI GACnn_nn'nnGTC
Cuts at: 0 642 2287 2331
Size: 642 1645 44
Fragments arranged by size:
1645 642 44
EaeI y'GGCC_r
Cuts at: 0 195 770 1906 2117 2331
Size: 195 575 1136 211 214
Fragments arranged by size:
1136 575 214 211 195
EarI CTCTTCn'nnn_
Cuts at: 0 307 392 832 2331
Size: 307 85 440 1499
Fragments arranged by size:
1499 440 307 85
EcoICRI GAG'CTC
Cuts at: 0 144 882 2331
Size: 144 738 1449
Fragments arranged by size:
1449 738 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 361 409 883 1073 1203 1219 1669
Size: 322 39 48 474 190 130 16 450
Cuts at: 1669 1803 2331
Size: 134 528
Fragments arranged by size:
528 474 450 322 190 134 130 48
39 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1782 2331
Size: 220 1562 549
Fragments arranged by size:
1562 549 220
EcoO109I rG'GnC_Cy
Cuts at: 0 30 765 934 1929 1930 2331
Size: 30 735 169 995 1 401
Fragments arranged by size:
995 735 401 169 30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 83 2331
Size: 83 2248
HaeII r_GCGC'y
Cuts at: 0 162 1677 2331
Size: 162 1515 654
Fragments arranged by size:
1515 654 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 979 1011 1515 1547 2227 2259
Size: 562 32 385 32 504 32 680 32
Cuts at: 2259 2331
Size: 72
Fragments arranged by size:
680 562 504 385 72 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 854 2331
Size: 854 1477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1293 1661 2331
Size: 1293 368 670
Fragments arranged by size:
1293 670 368
MscI TGG'CCA
Cuts at: 0 197 772 2331
Size: 197 575 1559
Fragments arranged by size:
1559 575 197
MslI CAynn'nnrTG
Cuts at: 0 1391 2331
Size: 1391 940
MspA1I CmG'CkG
Cuts at: 0 74 131 1223 1304 1373 1889 2331
Size: 74 57 1092 81 69 516 442
Fragments arranged by size:
1092 516 442 81 74 69 57
NspI r_CATG'y
Cuts at: 0 1624 2331
Size: 1624 707
PflMI CCAn_nnn'nTGG
Cuts at: 0 978 2331
Size: 978 1353
PfoI T'CCnGG_A
Cuts at: 0 377 1019 2331
Size: 377 642 1312
Fragments arranged by size:
1312 642 377
PpuMI rG'GwC_Cy
Cuts at: 0 765 2331
Size: 765 1566
PspOMI G'GGCC_C
Cuts at: 0 933 1929 2331
Size: 933 996 402
Fragments arranged by size:
996 933 402
PstI C_TGCA'G
Cuts at: 0 115 122 942 1378 2331
Size: 115 7 820 436 953
Fragments arranged by size:
953 820 436 115 7
PvuII CAG'CTG
Cuts at: 0 1304 1889 2331
Size: 1304 585 442
Fragments arranged by size:
1304 585 442
SacI G_AGCT'C
Cuts at: 0 146 884 2331
Size: 146 738 1447
Fragments arranged by size:
1447 738 146
ScaI AGT'ACT
Cuts at: 0 1567 2331
Size: 1567 764
SexAI A'CCwGG_T
Cuts at: 0 243 2331
Size: 243 2088
SfcI C'TryA_G
Cuts at: 0 111 118 938 1374 2331
Size: 111 7 820 436 957
Fragments arranged by size:
957 820 436 111 7
SmaI CCC'GGG
Cuts at: 0 1178 2331
Size: 1178 1153
SmlI C'TyrA_G
Cuts at: 0 760 883 2331
Size: 760 123 1448
Fragments arranged by size:
1448 760 123
StuI AGG'CCT
Cuts at: 0 1420 2124 2331
Size: 1420 704 207
Fragments arranged by size:
1420 704 207
StyI C'CwwG_G
Cuts at: 0 837 875 1725 1736 2331
Size: 837 38 850 11 595
Fragments arranged by size:
850 837 595 38 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 985 2124 2331
Size: 985 1139 207
Fragments arranged by size:
1139 985 207
TatI w'GTAC_w
Cuts at: 0 359 1565 2331
Size: 359 1206 766
Fragments arranged by size:
1206 766 359
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1149 1181 2060 2092 2331
Size: 1149 32 879 32 239
Fragments arranged by size:
1149 879 239 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 784 2331
Size: 784 1547
XmaI C'CCGG_G
Cuts at: 0 1176 2331
Size: 1176 1155
XmnI GAAnn'nnTTC
Cuts at: 0 476 868 2179 2331
Size: 476 392 1311 152
Fragments arranged by size:
1311 476 392 152
ZraI GAC'GTC
Cuts at: 0 1826 2331
Size: 1826 505
Enzymes that do cut:
AarI AatII AccI AcuI AflII AgeI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BciVI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstYI BstZ17I BtgI
BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NspI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI ScaI SexAI SfcI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AhdI AleI AlfI AloI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BfrBI
BglII BmgBI BmtI BplI BsaAI BsiEI BsiWI BsmI
BsmBI BssHII BstBI BstXI Bsu36I BtgZI CspCI EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XcmI XhoI