Restriction map for
pFN21ASDA1585
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbsI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 36 CGCG CG^CG
AcsI 1136 AAATTT [GA]^AATT[CT]
ApoI 1136 AAATTT [GA]^AATT[CT]
Asp700I 1306 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 49 GTGCTC G[AT]GC[AT]^C
BalI 1508 TGGCCA TGG^CCA
BamHI 1243 GGATCC G^GATCC
BbvCI 543 CCTCAGC CCTCAGC(-5/-2)
BciVI 892 GTATCC GTATCC(6/5)
BcnI 64 CCCGG CC^[GC]GG
BmyI 49 GTGCTC G[AGT]GC[ACT]^C
BsaBI 1025 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 68 GAGACC GGTCTC(1/5)
BseRI 421 GAGGAG GAGGAG(10/8)
BsgI 962 CTGCAC GTGCAG(16/14)
BsiHKAI 49 GTGCTC G[AT]GC[AT]^C
BsmI 12 GAATGC GAATGC(1/-1)
Bsp1286I 49 GTGCTC G[AGT]GC[ACT]^C
BspHI 1233 TCATGA T^CATGA
BspMI 181 ACCTGC ACCTGC(4/8)
BstAPI 1460 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 36 CGCG CG^CG
DraII 1648 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 1508 TGGCCA [CT]^GGCC[GA]
EarI 1142 CTCTTC CTCTTC(1/4)
EcoO109I 1648 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 754 GATATC GAT^ATC
HapII 65 CCGG C^CGG
HpaII 65 CCGG C^CGG
HphI 631 TCACC GGTGA(8/7)
Ksp632I 1142 CTCTTC CTCTTC(1/4)
MamI 1025 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1508 TGGCCA TGG^CCA
MscI 1508 TGGCCA TGG^CCA
MspI 65 CCGG C^CGG
MvnI 36 CGCG CG^CG
NciI 64 CCCGG CC^[GC]GG
NspI 1423 ACATGC [GA]CATG^[CT]
PpuMI 1648 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1233 TCATGA T^CATGA
ScaI 816 AGTACT AGT^ACT
SmlI 509 CTCAAG C^T[CT][GA]AG
SspI 622 AATATT AAT^ATT
StuI 1553 AGGCCT AGG^CCT
TaqI 1724 TCGA T^CGA
TthHB8I 1724 TCGA T^CGA
XcmI 1568 CCAGGCTCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1306 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 901 GGACC G^G[AT]CC
1649 GGTCC
BpmI 785 CTGGAG CTGGAG(16/14)
1528 CTGGAG
BsmFI 120 GTCCC GGGAC(10/14)
1650 GTCCC
Bsp1407I 982 TGTACA T^GTACA
1355 TGTACA
BsrDI 549 CATTGC GCAATG(2/0)
1599 CATTGC
BsrGI 982 TGTACA T^GTACA
1355 TGTACA
EcoT14I 74 CCAAGG C^C[AT][AT]GG
1556 CCTTGG
HincII 367 GTTGAC GT[CT]^[GA]AC
670 GTTGAC
HindII 367 GTTGAC GT[CT]^[GA]AC
670 GTTGAC
PleI 790 GACTC GAGTC(4/5)
1230 GAGTC
PstI 759 CTGCAG CTGCA^G
910 CTGCAG
SfaNI 290 GCATC GCATC(5/9)
1102 GCATC
SspBI 982 TGTACA T^GTACA
1355 TGTACA
StyI 74 CCAAGG C^C[AT][AT]GG
1556 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII