(Circular) (Six-base) MAPSORT of: pFN21ASDA1585.seq Check: 3704 from: 1 to: 6460 pFN21ASDA1585 6460 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2213 2213 Size: 6460 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3803 1937 Size: 1866 4594 Acc65I G'GTAC_C Cuts at: 3781 3781 Size: 6460 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4523 5907 6175 1632 Size: 2891 1384 268 1917 Fragments arranged by size: 2891 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6263 AflIII A'CryG_T Cuts at: 1203 5359 6336 1203 Size: 4156 977 1327 Fragments arranged by size: 4156 1327 977 AgeI A'CCGG_T Cuts at: 5291 5291 Size: 6460 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3644 1184 Size: 2460 4000 AlwNI CAG_nnn'CTG Cuts at: 4203 5775 4203 Size: 1572 4888 ApaLI G'TGCA_C Cuts at: 4517 5673 4517 Size: 1156 5304 ApoI r'AATT_y Cuts at: 1493 3159 3769 3995 4147 6256 1493 Size: 1666 610 226 152 2109 1697 Fragments arranged by size: 2109 1697 1666 610 226 152 AseI AT'TA_AT Cuts at: 160 1032 5014 160 Size: 872 3982 1606 Fragments arranged by size: 3982 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 3785 1870 Size: 73 1842 4545 Fragments arranged by size: 4545 1842 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3625 3658 3625 Size: 33 6427 BamHI G'GATC_C Cuts at: 3266 3790 4157 3266 Size: 524 367 5569 Fragments arranged by size: 5569 524 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3781 5236 618 Size: 325 217 627 1994 1455 1842 Fragments arranged by size: 1994 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3779 1391 Size: 2388 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5489 BbvCI CC'TCA_GC Cuts at: 2567 2567 Size: 6460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4651 4685 1903 Size: 34 2714 34 3678 Fragments arranged by size: 3678 2714 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2926 4352 5562 2926 Size: 1426 1210 3824 Fragments arranged by size: 3824 1426 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5071 136 Size: 107 122 71 631 605 3399 1525 Fragments arranged by size: 3399 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6455 6455 Size: 6460 BlpI GC'TnA_GC Cuts at: 3877 3877 Size: 6460 Bme1580I G_kGCm'C Cuts at: 1790 4521 5677 1790 Size: 2731 1156 2573 Fragments arranged by size: 2731 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2941 3128 3671 Size: 416 53 83 399 24 1524 187 543 Cuts at: 3671 4191 5149 442 Size: 520 958 1753 Fragments arranged by size: 1753 1524 958 543 520 416 399 187 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2829 3572 5120 1239 Size: 1590 743 1548 2579 Fragments arranged by size: 2579 1590 1548 743 Bpu10I CC'TnA_GC Cuts at: 2567 2567 Size: 6460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2517 4585 5450 5748 2517 Size: 2068 865 298 3229 Fragments arranged by size: 3229 2068 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2085 5123 882 Size: 617 586 3038 2219 Fragments arranged by size: 3038 2219 617 586 BsaAI yAC'GTr Cuts at: 493 1204 3757 4251 493 Size: 711 2553 494 2702 Fragments arranged by size: 2702 2553 711 494 BsaBI GATnn'nnATC Cuts at: 3052 6454 3052 Size: 3402 3058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4267 4649 5284 275 Size: 53 83 186 936 2734 382 635 1451 Fragments arranged by size: 2734 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4893 5291 5565 5712 6123 4893 Size: 398 274 147 411 5230 Fragments arranged by size: 5230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6235 Fragments arranged by size: 6235 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 1186 Size: 1273 5187 BseYI C'CCAG_C Cuts at: 5663 6159 5663 Size: 496 5964 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2970 1163 Size: 573 1234 4653 Fragments arranged by size: 4653 1234 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3910 4671 5699 1540 Size: 275 186 1909 761 1028 2301 Fragments arranged by size: 2301 1909 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 4521 4606 5677 2076 Size: 2445 85 1071 2859 Fragments arranged by size: 2859 2445 1071 85 BsmI GAATG_Cn' Cuts at: 2041 3971 4064 2041 Size: 1930 93 4437 Fragments arranged by size: 4437 1930 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 3779 4521 4606 5677 1391 Size: 399 286 1703 742 85 1071 2174 Fragments arranged by size: 2174 1703 1071 742 399 286 85 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 3256 4349 6079 3256 Size: 1093 1730 3637 Fragments arranged by size: 3637 1730 1093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2213 3815 844 Size: 1369 1602 3489 Fragments arranged by size: 3489 1602 1369 BsrBI CCG'CTC Cuts at: 1394 1823 4347 1394 Size: 429 2524 3507 Fragments arranged by size: 3507 2524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 3621 4955 5129 65 Size: 1183 1323 1050 1334 174 1396 Fragments arranged by size: 1396 1334 1323 1183 1050 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5104 5291 1700 Size: 110 3294 187 2869 Fragments arranged by size: 3294 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3005 3378 96 Size: 2909 373 3178 Fragments arranged by size: 3178 2909 373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 4520 5532 4520 Size: 1012 5448 BstAPI GCAn_nnn'nTGC Cuts at: 794 3489 794 Size: 2695 3765 BstBI TT'CG_AA Cuts at: 2007 5264 2007 Size: 3257 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstYI r'GATC_y Cuts at: 1972 2179 2710 3266 3790 4157 4542 4559 6011 Size: 207 531 556 524 367 385 17 1452 Cuts at: 6011 6097 6109 6455 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 556 531 524 385 367 346 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5908 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5721 Fragments arranged by size: 5721 683 56 BtsI GCAGTG_nn' Cuts at: 3976 4770 4790 3976 Size: 794 20 5646 Fragments arranged by size: 5646 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2755 2790 529 Size: 35 2191 35 4199 Fragments arranged by size: 4199 2191 35 35 DraI TTT'AAA Cuts at: 3764 3764 Size: 6460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5467 809 Size: 507 4151 1802 Fragments arranged by size: 4151 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3531 3907 4796 Size: 54 1425 50 275 18 1701 376 889 Cuts at: 4796 6425 8 Size: 1629 43 Fragments arranged by size: 1701 1629 1425 889 376 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3907 1537 Size: 275 2095 4090 Fragments arranged by size: 4090 2095 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3171 4390 1353 Size: 1818 1219 3423 Fragments arranged by size: 3423 1818 1219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5047 5421 5567 387 Size: 1064 138 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2829 3572 4523 5120 5907 6175 1239 Size: 393 1197 743 951 597 787 268 1524 Fragments arranged by size: 1524 1197 951 787 743 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3672 3672 Size: 6460 EcoRI G'AATT_C Cuts at: 3769 4147 3769 Size: 378 6082 EcoRV GAT'ATC Cuts at: 2779 2779 Size: 6460 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 5607 1678 Size: 3929 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2699 2731 3325 3357 5101 Size: 32 135 32 1127 32 594 32 1744 Cuts at: 5101 5133 5175 5207 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1744 1127 594 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2392 2695 3804 4050 669 Size: 988 281 454 303 1109 246 3079 Fragments arranged by size: 3079 1109 988 454 303 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6460 HpaI GTT'AAC Cuts at: 4050 4050 Size: 6460 KpnI G_GTAC'C Cuts at: 3785 3785 Size: 6460 MfeI C'AATT_G Cuts at: 4059 4059 Size: 6460 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2161 5574 5758 1305 Size: 856 3413 184 2007 Fragments arranged by size: 3413 2007 856 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3533 10 Size: 54 1425 343 1701 2937 Fragments arranged by size: 2937 1701 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 3261 3611 3626 4418 4777 4936 Size: 648 1152 943 350 15 792 359 159 Cuts at: 4936 518 Size: 2042 Fragments arranged by size: 2042 1152 943 792 648 359 350 159 15 MspA1I CmG'CkG Cuts at: 1606 3876 4553 5701 5946 1606 Size: 2270 677 1148 245 2120 Fragments arranged by size: 2270 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5908 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NspI r_CATG'y Cuts at: 1132 1229 3450 3818 5363 1132 Size: 97 2221 368 1545 2229 Fragments arranged by size: 2229 2221 1545 368 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 5359 5359 Size: 6460 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6460 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4529 4561 6042 6074 4529 Size: 32 1481 32 4915 Fragments arranged by size: 4915 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3672 3672 Size: 6460 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 2613 4030 2613 Size: 1417 5043 PstI C_TGCA'G Cuts at: 830 2786 2937 3812 830 Size: 1956 151 875 3478 Fragments arranged by size: 3478 1956 875 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SanDI GG'GwC_CC Cuts at: 3672 3672 Size: 6460 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 2841 4708 6420 2841 Size: 1867 1712 2881 Fragments arranged by size: 2881 1867 1712 SfcI C'TryA_G Cuts at: 826 1045 2263 2782 2933 3174 3732 3808 4943 Size: 219 1218 519 151 241 558 76 1135 Cuts at: 4943 5624 5815 826 Size: 681 191 1471 Fragments arranged by size: 1471 1218 1135 681 558 519 241 219 191 151 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2532 4564 5465 5727 820 Size: 197 926 589 2032 901 262 1553 Fragments arranged by size: 2032 1553 926 901 589 262 197 SnaBI TAC'GTA Cuts at: 493 3757 4251 493 Size: 3264 494 2702 Fragments arranged by size: 3264 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SspI AAT'ATT Cuts at: 5 52 2647 4384 5 Size: 47 2595 1737 2081 Fragments arranged by size: 2595 2081 1737 47 StuI AGG'CCT Cuts at: 1517 3578 1517 Size: 2061 4399 StyI C'CwwG_G Cuts at: 513 1065 2097 3579 3899 513 Size: 552 1032 1482 320 3074 Fragments arranged by size: 3074 1482 1032 552 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2206 4487 4504 4657 4842 5287 325 Size: 1255 626 2281 17 153 185 445 1498 Fragments arranged by size: 2281 1498 1255 626 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2839 3005 3223 Size: 274 80 33 51 1443 862 166 218 Cuts at: 3223 3378 3701 4706 6418 96 Size: 155 323 1005 1712 138 Fragments arranged by size: 1712 1443 1005 862 323 274 218 166 155 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2512 2544 6420 6452 2512 Size: 32 3876 32 2520 Fragments arranged by size: 3876 2520 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6460 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3598 3598 Size: 6460 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 3333 4589 3333 Size: 1256 5204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI