(Circular) (Six-base) MAPSORT of: pFN21ASDA1581.seq Check: 6527 from: 1 to: 6205 pFN21ASDA1581 6205 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3375 3548 1937 Size: 1438 173 4594 Fragments arranged by size: 4594 1438 173 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3224 4268 5652 5920 1632 Size: 1592 1044 1384 268 1917 Fragments arranged by size: 1917 1592 1384 1044 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 3063 5104 6081 1203 Size: 1860 2041 977 1327 Fragments arranged by size: 2041 1860 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 3948 5520 3948 Size: 1572 4633 ApaLI G'TGCA_C Cuts at: 3290 4262 5418 3290 Size: 972 1156 4077 Fragments arranged by size: 4077 1156 972 ApoI r'AATT_y Cuts at: 1493 2030 3514 3740 3892 6001 1493 Size: 537 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2811 3530 1870 Size: 73 868 719 4545 Fragments arranged by size: 4545 868 719 73 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 3526 4981 618 Size: 325 217 627 287 1452 1455 1842 Fragments arranged by size: 1842 1455 1452 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 2589 3317 3524 1391 Size: 1198 728 207 4072 Fragments arranged by size: 4072 1198 728 207 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3432 928 Size: 971 1533 3701 Fragments arranged by size: 3701 1533 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2345 2931 4097 5307 2345 Size: 586 1166 1210 3243 Fragments arranged by size: 3243 1210 1166 586 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2843 4816 136 Size: 107 122 71 631 605 1171 1973 1525 Fragments arranged by size: 1973 1525 1171 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 2735 3622 2735 Size: 887 5318 Bme1580I G_kGCm'C Cuts at: 1790 2079 3294 4266 5422 1790 Size: 289 1215 972 1156 2573 Fragments arranged by size: 2573 1215 1156 972 289 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3149 3936 4894 Size: 416 53 83 399 24 1732 787 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 1753 1732 958 787 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4865 1239 Size: 3626 2579 Bpu10I CC'TnA_GC Cuts at: 2535 2535 Size: 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3502 3996 493 Size: 711 2298 494 2702 Fragments arranged by size: 2702 2298 711 494 BsaBI GATnn'nnATC Cuts at: 3086 6199 3086 Size: 3113 3092 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4012 4394 5029 275 Size: 53 83 186 936 2479 382 635 1451 Fragments arranged by size: 2479 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2064 3378 4638 5036 5310 5457 5868 2064 Size: 1314 1260 398 274 147 411 2401 Fragments arranged by size: 2401 1314 1260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3392 3422 1178 Size: 30 165 30 1989 30 3961 Fragments arranged by size: 3961 1989 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3473 3503 1186 Size: 2287 30 3888 Fragments arranged by size: 3888 2287 30 BseYI C'CCAG_C Cuts at: 2704 2945 3227 5408 5904 2704 Size: 241 282 2181 496 3005 Fragments arranged by size: 3005 2181 496 282 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 2620 1163 Size: 573 621 263 4748 Fragments arranged by size: 4748 621 573 263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2966 3294 4266 4351 5422 2966 Size: 328 972 85 1071 3749 Fragments arranged by size: 3749 1071 972 328 85 BsmI GAATG_Cn' Cuts at: 2472 2582 3716 3809 2472 Size: 110 1134 93 4868 Fragments arranged by size: 4868 1134 110 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2589 2966 3294 3317 3524 4266 Size: 399 289 510 377 328 23 207 742 Cuts at: 4266 4351 5422 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 399 377 328 289 207 85 23 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 2196 4094 5824 2196 Size: 1898 1730 2577 Fragments arranged by size: 2577 1898 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2133 2457 4700 4874 65 Size: 1183 885 324 2243 174 1396 Fragments arranged by size: 2243 1396 1183 885 324 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2613 96 Size: 2517 3688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 2965 3266 4265 5277 2965 Size: 301 999 1012 3893 Fragments arranged by size: 3893 1012 999 301 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 2007 5009 2007 Size: 3002 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2895 3268 2895 Size: 373 5832 BstYI r'GATC_y Cuts at: 1972 3138 3535 3902 4287 4304 5756 5842 5854 Size: 1166 397 367 385 17 1452 86 12 Cuts at: 5854 6200 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1166 397 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3348 3414 513 Size: 552 957 1326 66 3304 Fragments arranged by size: 3304 1326 957 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2293 3241 3343 487 Size: 683 56 1067 948 102 3349 Fragments arranged by size: 3349 1067 948 683 102 56 BtsI GCAGTG_nn' Cuts at: 2853 3721 4515 4535 2853 Size: 868 794 20 4523 Fragments arranged by size: 4523 868 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 2380 2646 3189 2380 Size: 266 543 5396 Fragments arranged by size: 5396 543 266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2766 3411 Size: 54 1425 50 275 18 339 597 645 Cuts at: 3411 3652 4541 6170 8 Size: 241 889 1629 43 Fragments arranged by size: 1629 1425 889 645 597 339 275 241 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4135 1353 Size: 2782 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3224 4268 4865 5652 5920 1239 Size: 393 1592 1044 597 787 268 1524 Fragments arranged by size: 1592 1524 1044 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2213 2412 2815 2213 Size: 199 403 5603 Fragments arranged by size: 5603 403 199 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3022 3229 3022 Size: 207 5998 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2042 5352 1678 Size: 364 3310 2531 Fragments arranged by size: 3310 2531 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2767 2799 3163 3195 3392 Size: 32 135 32 1195 32 364 32 197 Cuts at: 3392 3424 4846 4878 4920 4952 1373 Size: 32 1422 32 42 32 2626 Fragments arranged by size: 2626 1422 1195 364 197 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2797 2901 3549 3795 669 Size: 988 281 859 104 648 246 3079 Fragments arranged by size: 3079 988 859 648 281 246 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3476 5319 5503 1305 Size: 2171 1843 184 2007 Fragments arranged by size: 2171 2007 1843 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 2768 10 Size: 54 1425 343 339 597 3447 Fragments arranged by size: 3447 1425 597 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 3095 4163 4522 4681 518 Size: 648 1929 1068 359 159 2042 Fragments arranged by size: 2042 1929 1068 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3621 4298 5446 5691 1606 Size: 2015 677 1148 245 2120 Fragments arranged by size: 2120 2015 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3348 513 Size: 552 957 1326 3370 Fragments arranged by size: 3370 1326 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2887 3448 3563 5108 1132 Size: 97 1658 561 115 1545 2229 Fragments arranged by size: 2229 1658 1545 561 115 97 PasI CC'CwG_GG Cuts at: 1348 2080 3304 1348 Size: 732 1224 4249 Fragments arranged by size: 4249 1224 732 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2213 2815 2213 Size: 602 5603 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PstI C_TGCA'G Cuts at: 830 2836 3557 830 Size: 2006 721 3478 Fragments arranged by size: 3478 2006 721 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SanDI GG'GwC_CC Cuts at: 2213 2213 Size: 6205 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2832 3553 4688 5369 5560 826 Size: 219 1787 721 1135 681 191 1471 Fragments arranged by size: 1787 1471 1135 721 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 2813 3532 1872 Size: 941 719 4545 Fragments arranged by size: 4545 941 719 SmlI C'TyrA_G Cuts at: 820 1017 1943 4309 5210 5472 820 Size: 197 926 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3502 3996 493 Size: 3009 494 2702 Fragments arranged by size: 3009 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 2120 1517 Size: 603 5602 StyI C'CwwG_G Cuts at: 513 1065 2022 2818 3348 3644 513 Size: 552 957 796 530 296 3074 Fragments arranged by size: 3074 957 796 552 530 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3073 4232 4249 4402 4587 5032 325 Size: 1255 1493 1159 17 153 185 445 1498 Fragments arranged by size: 1498 1493 1255 1159 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 4451 6163 Size: 274 80 33 51 1443 636 1838 1712 Cuts at: 6163 96 Size: 138 Fragments arranged by size: 1838 1712 1443 636 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6165 6197 6165 Size: 32 6173 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2647 2845 2872 2953 2179 Size: 468 198 27 81 5431 Fragments arranged by size: 5431 468 198 81 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 2811 3530 1870 Size: 941 719 4545 Fragments arranged by size: 4545 941 719 XmnI GAAnn'nnTTC Cuts at: 2204 3212 4334 2204 Size: 1008 1122 4075 Fragments arranged by size: 4075 1122 1008 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI