(Circular) (Six-base) MAPSORT of: pFN21ASDA1576.seq Check: 9392 from: 1 to: 5986 pFN21ASDA1576 5986 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2902 2902 Size: 5986 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2460 3329 1937 Size: 523 869 4594 Fragments arranged by size: 4594 869 523 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 2775 2872 4049 5433 5701 1632 Size: 550 593 97 1177 1384 268 1917 Fragments arranged by size: 1917 1384 1177 593 550 268 97 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 2447 1166 Size: 1281 4705 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 3729 5301 3729 Size: 1572 4414 ApaLI G'TGCA_C Cuts at: 2579 4043 5199 2579 Size: 1464 1156 3366 Fragments arranged by size: 3366 1464 1156 ApoI r'AATT_y Cuts at: 1493 3295 3521 3673 5782 1493 Size: 1802 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2129 2213 3311 1870 Size: 73 186 84 1098 4545 Fragments arranged by size: 4545 1098 186 84 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2323 3307 4762 618 Size: 325 217 627 536 984 1455 1842 Fragments arranged by size: 1842 1455 984 627 536 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2235 3045 3091 3305 1391 Size: 790 54 810 46 214 4072 Fragments arranged by size: 4072 810 790 214 54 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2807 928 Size: 971 908 4107 Fragments arranged by size: 4107 971 908 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2480 2514 4177 4211 1903 Size: 34 543 34 1663 34 3678 Fragments arranged by size: 3678 1663 543 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3006 4597 136 Size: 107 122 71 631 605 1334 1591 1525 Fragments arranged by size: 1591 1525 1334 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 2616 3403 2616 Size: 787 5199 Bme1580I G_kGCm'C Cuts at: 1790 2583 4047 5203 1790 Size: 793 1464 1156 2573 Fragments arranged by size: 2573 1464 1156 793 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2889 3113 4646 1239 Size: 1650 224 1533 2579 Fragments arranged by size: 2579 1650 1533 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 4111 4976 5274 2219 Size: 1892 865 298 2931 Fragments arranged by size: 2931 1892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2385 2813 2867 3197 4649 882 Size: 617 886 428 54 330 1452 2219 Fragments arranged by size: 2219 1452 886 617 428 330 54 BsaAI yAC'GTr Cuts at: 493 1204 2728 3283 3777 493 Size: 711 1524 555 494 2702 Fragments arranged by size: 2702 1524 711 555 494 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 3793 4175 4810 Size: 53 83 186 936 603 1657 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1657 1451 936 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2382 2412 3160 3190 1178 Size: 30 165 30 979 30 748 30 3974 Fragments arranged by size: 3974 979 748 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2960 3110 3239 3242 1186 Size: 880 894 150 129 3 3930 Fragments arranged by size: 3930 894 880 150 129 3 BseYI C'CCAG_C Cuts at: 5189 5685 5189 Size: 496 5490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2425 1163 Size: 573 689 4724 Fragments arranged by size: 4724 689 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3436 4197 5225 1540 Size: 275 186 1129 306 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 306 275 186 BsiHKAI G_wGCw'C Cuts at: 2235 2577 2583 4047 4132 5203 2235 Size: 342 6 1464 85 1071 3018 Fragments arranged by size: 3018 1464 1071 342 85 6 BsmI GAATG_Cn' Cuts at: 2501 3497 3590 2501 Size: 996 93 4897 Fragments arranged by size: 4897 996 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2048 2048 Size: 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2235 2577 2583 3045 3091 3305 Size: 399 391 54 342 6 462 46 214 Cuts at: 3305 4047 4132 5203 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 462 399 391 342 214 85 54 46 6 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2902 3341 844 Size: 2058 439 3489 Fragments arranged by size: 3489 2058 439 BsrBI CCG'CTC Cuts at: 1394 1823 3262 3873 1394 Size: 429 1439 611 3507 Fragments arranged by size: 3507 1439 611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 4630 4817 1700 Size: 110 282 2538 187 2869 Fragments arranged by size: 2869 2538 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 2149 1925 Size: 224 5762 BssSI C'ACGA_G Cuts at: 2576 3236 4046 5058 2576 Size: 660 810 1012 3504 Fragments arranged by size: 3504 1012 810 660 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 2007 4790 2007 Size: 2783 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2238 2466 3316 3683 4068 4085 5537 5623 Size: 266 228 850 367 385 17 1452 86 Cuts at: 5623 5635 5981 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 850 385 367 346 266 228 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2220 2220 Size: 5986 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4477 Fragments arranged by size: 4477 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2944 487 Size: 683 56 1718 3529 Fragments arranged by size: 3529 1718 683 56 BtsI GCAGTG_nn' Cuts at: 3502 4296 4316 3502 Size: 794 20 5172 Fragments arranged by size: 5172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2236 2271 529 Size: 35 1672 35 4244 Fragments arranged by size: 4244 1672 35 35 DraI TTT'AAA Cuts at: 2979 3290 2979 Size: 311 5675 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 3186 3433 Size: 54 1425 50 275 18 195 1161 247 Cuts at: 3433 4322 5951 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1161 889 275 247 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2993 3216 3916 1353 Size: 848 792 223 700 3423 Fragments arranged by size: 3423 848 792 700 223 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 2111 4573 4947 5093 387 Size: 1064 138 474 48 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 474 374 146 138 48 EcoICRI GAG'CTC Cuts at: 2233 2233 Size: 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2775 2872 2889 3113 4049 4646 Size: 393 550 593 97 17 224 936 597 Cuts at: 4646 5433 5701 1239 Size: 787 268 1524 Fragments arranged by size: 1524 936 787 597 593 550 393 268 224 97 17 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2902 2902 Size: 5986 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2382 2414 3160 3192 4627 Size: 32 135 32 810 32 746 32 1435 Cuts at: 4627 4659 4701 4733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1435 810 746 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2880 5100 5284 1305 Size: 1575 2220 184 2007 Fragments arranged by size: 2220 2007 1575 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 3188 10 Size: 54 1425 343 195 1161 2808 Fragments arranged by size: 2808 1425 1161 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 3944 4303 4462 518 Size: 648 1281 1497 359 159 2042 Fragments arranged by size: 2042 1497 1281 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2588 2620 3118 3402 4079 5227 5472 1606 Size: 982 32 498 284 677 1148 245 2120 Fragments arranged by size: 2120 1148 982 677 498 284 245 32 NaeI GCC'GGC Cuts at: 2094 2094 Size: 5986 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4477 Fragments arranged by size: 4477 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NgoMIV G'CCGG_C Cuts at: 2092 2092 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 2827 1348 Size: 1479 4507 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PfoI T'CCnGG_A Cuts at: 2469 2556 2469 Size: 87 5899 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2647 2679 2647 Size: 32 5954 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2588 2620 2588 Size: 32 5954 SacI G_AGCT'C Cuts at: 2235 2235 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SapI GCTCTTCn'nnn_ Cuts at: 2201 2993 2201 Size: 792 5194 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 3334 4469 5150 5341 826 Size: 219 2289 1135 681 191 1471 Fragments arranged by size: 2289 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3006 3006 Size: 5986 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 4090 4991 5253 820 Size: 197 926 291 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 3283 3777 493 Size: 2790 494 2702 Fragments arranged by size: 2790 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 2296 3910 5 Size: 47 2244 1614 2081 Fragments arranged by size: 2244 2081 1614 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2022 2028 3425 513 Size: 552 957 6 1397 3074 Fragments arranged by size: 3074 1397 957 552 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3144 4013 4030 4183 4368 4813 325 Size: 1255 1564 869 17 153 185 445 1498 Fragments arranged by size: 1564 1498 1255 869 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3068 3100 5946 5978 3068 Size: 32 2846 32 3076 Fragments arranged by size: 3076 2846 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2396 1316 Size: 1080 4906 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2317 2035 Size: 282 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfoI SrfI SwaI