(Circular) (Six-base) MAPSORT of: pFN21ASDA1572.seq Check: 3316 from: 1 to: 6052 pFN21ASDA1572 6052 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2817 2923 3395 1937 Size: 880 106 472 4594 Fragments arranged by size: 4594 880 472 106 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 2550 4115 5499 5767 1632 Size: 704 214 1565 1384 268 1917 Fragments arranged by size: 1917 1565 1384 704 268 214 AflII C'TTAA_G Cuts at: 820 1017 2194 3319 820 Size: 197 1177 1125 3553 Fragments arranged by size: 3553 1177 1125 197 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 4781 4781 Size: 6052 AleI CACnn'nnGTG Cuts at: 1166 2973 1166 Size: 1807 4245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2499 1184 Size: 1315 4737 AlwNI CAG_nnn'CTG Cuts at: 2389 3283 3795 5367 2389 Size: 894 512 1572 3074 Fragments arranged by size: 3074 1572 894 512 ApaLI G'TGCA_C Cuts at: 2377 4109 5265 2377 Size: 1732 1156 3164 Fragments arranged by size: 3164 1732 1156 ApoI r'AATT_y Cuts at: 1493 2114 2303 2686 3361 3587 3739 5848 1493 Size: 621 189 383 675 226 152 2109 1697 Fragments arranged by size: 2109 1697 675 621 383 226 189 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 3377 1870 Size: 73 1434 4545 Fragments arranged by size: 4545 1434 73 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 4828 618 Size: 325 217 627 1586 1455 1842 Fragments arranged by size: 1842 1586 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2670 3371 1391 Size: 1279 701 4072 Fragments arranged by size: 4072 1279 701 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2538 928 Size: 971 639 4442 Fragments arranged by size: 4442 971 639 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4663 136 Size: 107 122 71 631 605 2991 1525 Fragments arranged by size: 2991 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2038 6047 2038 Size: 4009 2043 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2381 2849 4113 5269 1790 Size: 591 468 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 591 468 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2455 3783 4741 Size: 416 53 83 399 24 1038 1328 958 Cuts at: 4741 442 Size: 1753 Fragments arranged by size: 1753 1328 1038 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2472 2472 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2897 4712 1239 Size: 1658 1815 2579 Fragments arranged by size: 2579 1815 1658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4177 5042 5340 4177 Size: 865 298 4889 Fragments arranged by size: 4889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2193 4715 882 Size: 617 694 2522 2219 Fragments arranged by size: 2522 2219 694 617 BsaAI yAC'GTr Cuts at: 493 1204 3349 3843 493 Size: 711 2145 494 2702 Fragments arranged by size: 2702 2145 711 494 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4485 4883 5157 5304 5715 4485 Size: 398 274 147 411 4822 Fragments arranged by size: 4822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5827 Fragments arranged by size: 5827 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6052 BseYI C'CCAG_C Cuts at: 2670 5255 5751 2670 Size: 2585 496 2971 Fragments arranged by size: 2971 2585 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5479 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2381 4113 4198 5269 2381 Size: 1732 85 1071 3164 Fragments arranged by size: 3164 1732 1071 85 BsmI GAATG_Cn' Cuts at: 3563 3656 3563 Size: 93 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2381 2670 2849 3371 4113 4198 5269 Size: 399 591 289 179 522 742 85 1071 Cuts at: 5269 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 591 522 399 289 179 85 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2171 3281 3407 844 Size: 1327 1110 126 3489 Fragments arranged by size: 3489 1327 1110 126 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4696 4883 1700 Size: 110 2886 187 2869 Fragments arranged by size: 2886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3190 3227 96 Size: 3094 37 2921 Fragments arranged by size: 3094 2921 37 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 4112 5124 4112 Size: 1012 5040 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6052 BstBI TT'CG_AA Cuts at: 2007 4856 2007 Size: 2849 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2038 3382 3749 4134 4151 5603 5689 5701 Size: 66 1344 367 385 17 1452 86 12 Cuts at: 5701 6047 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1344 385 367 346 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5500 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 2646 2767 3568 4362 4382 2646 Size: 121 801 794 20 4316 Fragments arranged by size: 4316 801 794 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 2898 3356 2898 Size: 458 5594 DraIII CAC_nnn'GTG Cuts at: 2450 2450 Size: 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5059 809 Size: 507 3743 1802 Fragments arranged by size: 3743 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3499 4388 6017 Size: 54 1425 50 275 18 1669 889 1629 Cuts at: 6017 8 Size: 43 Fragments arranged by size: 1669 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 3982 1353 Size: 747 1882 3423 Fragments arranged by size: 3423 1882 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4639 5013 5159 387 Size: 1064 138 3050 374 146 1280 Fragments arranged by size: 3050 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2550 2897 4115 4712 5499 5767 Size: 393 704 214 347 1218 597 787 268 Cuts at: 5767 1239 Size: 1524 Fragments arranged by size: 1524 1218 787 704 597 393 347 268 214 EcoO109I rG'GnC_Cy Cuts at: 2386 3280 2386 Size: 894 5158 EcoRI G'AATT_C Cuts at: 2303 3361 3739 2303 Size: 1058 378 4616 Fragments arranged by size: 4616 1058 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2975 3058 2975 Size: 83 5969 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 5199 1678 Size: 3521 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2259 2291 2929 Size: 32 135 32 453 32 202 32 638 Cuts at: 2929 2961 4693 4725 4767 4799 1373 Size: 32 1732 32 42 32 2626 Fragments arranged by size: 2626 1732 638 453 202 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 2270 3651 2270 Size: 1381 4671 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5166 5350 1305 Size: 3861 184 2007 Fragments arranged by size: 3861 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4230 Fragments arranged by size: 4230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2739 2774 2973 2975 4010 4369 4528 Size: 648 1573 35 199 2 1035 359 159 Cuts at: 4528 518 Size: 2042 Fragments arranged by size: 2042 1573 1035 648 359 199 159 35 2 MspA1I CmG'CkG Cuts at: 1606 3148 3468 4145 5293 5538 1606 Size: 1542 320 677 1148 245 2120 Fragments arranged by size: 2120 1542 1148 677 320 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5500 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2974 2984 3228 3410 4955 1132 Size: 97 1745 10 244 182 1545 2229 Fragments arranged by size: 2229 1745 1545 244 182 97 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6052 PfoI T'CCnGG_A Cuts at: 3282 3282 Size: 6052 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3280 3280 Size: 6052 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 2402 3622 2402 Size: 1220 4832 PstI C_TGCA'G Cuts at: 830 2182 3404 830 Size: 1352 1222 3478 Fragments arranged by size: 3478 1352 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 3148 3148 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 3334 4300 6012 3334 Size: 966 1712 3374 Fragments arranged by size: 3374 1712 966 SfcI C'TryA_G Cuts at: 826 1045 2178 2828 3400 4535 5216 5407 826 Size: 219 1133 650 572 1135 681 191 1471 Fragments arranged by size: 1471 1135 1133 681 650 572 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 3379 1872 Size: 1507 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2194 3319 4156 5057 5319 820 Size: 197 926 251 1125 837 901 262 1553 Fragments arranged by size: 1553 1125 926 901 837 262 251 197 SnaBI TAC'GTA Cuts at: 493 3349 3843 493 Size: 2856 494 2702 Fragments arranged by size: 2856 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 2984 3410 1132 Size: 1852 426 3774 Fragments arranged by size: 3774 1852 426 SspI AAT'ATT Cuts at: 5 52 2806 3976 5 Size: 47 2754 1170 2081 Fragments arranged by size: 2754 2081 1170 47 StuI AGG'CCT Cuts at: 1517 2826 1517 Size: 1309 4743 StyI C'CwwG_G Cuts at: 513 1065 3491 513 Size: 552 2426 3074 Fragments arranged by size: 3074 2426 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4079 4096 4249 4434 4879 325 Size: 1255 2499 17 153 185 445 1498 Fragments arranged by size: 2499 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3190 3227 3332 Size: 274 80 33 51 1443 1213 37 105 Cuts at: 3332 4298 6010 96 Size: 966 1712 138 Fragments arranged by size: 1712 1443 1213 966 274 138 105 80 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6012 6044 6012 Size: 32 6020 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 3377 1870 Size: 1507 4545 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI