Restriction map for
pFN21ASDA1562
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AvaI AviII
AvrII BalI BanII BbeI BcgI
BfrI BlnI BsaHI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoT22I FauI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MluI MluNI MroI
MscI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 2176 CGCG CG^CG
AflIII 2837 ACATGT A^C[GA][CT]GT
AhdI 1323 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1469 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2114 CCTTAGG CC^T[ACGT]AGG
AseI 388 ATTAAT AT^TAAT
AsnI 388 ATTAAT AT^TAAT
Asp700I 1878 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1323 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1202 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2283 GGTGCC G^G[CT][GA]CC
BbrPI 2434 CACGTG CAC^GTG
BciVI 2991 GGATAC GTATCC(6/5)
BglI 2247 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2911 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2911 GCTCAGC GC^T[ACGT]AGC
BsaAI 2434 CACGTG [CT]AC^GT[GA]
BsaBI 1471 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2490 GGTCTC GGTCTC(1/5)
BsaWI 2926 TCCGGT [AT]^CCGG[AT]
BspHI 1602 TCATGA T^CATGA
BspLU11I 2837 ACATGT A^CATGT
BsrFI 2375 GCCGGC [GA]^CCGG[CT]
BstAPI 2122 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3142 GGTTACC G^GT[ACGT]ACC
BstPI 3142 GGTTACC G^GT[ACGT]ACC
BstUI 2176 CGCG CG^CG
Bsu36I 2114 CCTTAGG CC^T[ACGT]AGG
CelII 2911 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2375 GCCGGC [GA]^CCGG[CT]
Eam1105I 1323 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2940 GAAGAG CTCTTC(1/4)
Eco81I 2114 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3142 GGTTACC G^GT[ACGT]ACC
HaeII 826 GGCGCT [GA]GCGC^[CT]
Ksp632I 2940 GAAGAG CTCTTC(1/4)
MamI 1471 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1848 CAGCTG C[AC]G^C[GT]G
MvnI 2176 CGCG CG^CG
NaeI 2375 GCCGGC GCC^GGC
NcoI 3263 CCATGG C^CATGG
NgoMIV 2375 GCCGGC G^CCGGC
NspI 2837 ACATGT [GA]CATG^[CT]
PflFI 1202 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2434 CACGTG CAC^GTG
PmlI 2434 CACGTG CAC^GTG
PshBI 388 ATTAAT AT^TAAT
PvuII 1848 CAGCTG CAG^CTG
RcaI 1602 TCATGA T^CATGA
SspI 2558 AATATT AAT^ATT
Tth111I 1202 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1878 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AclI 1714 AACGTT AA^CGTT
1921 AACGTT
AspHI 1687 GTGCTC G[AT]GC[AT]^C
3310 GTGCTC
BamHI 813 GGATCC G^GATCC
1116 GGATCC
BbsI 1224 GAAGAC GAAGAC(2/6)
2817 GTCTTC
BbvCI 1990 CCTCAGC CCTCAGC(-5/-2)
1309 GCTGAGG
BclI 329 TGATCA T^GATCA
1599 TGATCA
BcnI 777 CCCGG CC^[GC]GG
2252 CCCGG
BmyI 1687 GTGCTC G[AGT]GC[ACT]^C
3310 GTGCTC
BpmI 3228 CTCCAG CTGGAG(16/14)
3103 CTGGAG
BpuAI 1224 GAAGAC GAAGAC(2/6)
2817 GTCTTC
BsiHKAI 1687 GTGCTC G[AT]GC[AT]^C
3310 GTGCTC
BsmBI 2330 CGTCTC CGTCTC(1/5)
3040 CGTCTC
Bsp1286I 1687 GTGCTC G[AGT]GC[ACT]^C
3310 GTGCTC
BspMI 1864 GCAGGT ACCTGC(4/8)
2487 GCAGGT
DraI 889 TTTAAA TTT^AAA
1213 TTTAAA
EaeI 2213 TGGCCG [CT]^GGCC[GA]
2619 TGGCCG
EcoRI 2729 GAATTC G^AATTC
2857 GAATTC
EcoRV 435 GATATC GAT^ATC
1369 GATATC
EcoT14I 2084 CCTTGG C^C[AT][AT]GG
3263 CCATGG
FbaI 329 TGATCA T^GATCA
1599 TGATCA
HgaI 2908 GACGC GACGC(5/10)
3039 GCGTC
HindIII 169 AAGCTT A^AGCTT
1192 AAGCTT
MfeI 272 CAATTG C^AATTG
1814 CAATTG
MunI 272 CAATTG C^AATTG
1814 CAATTG
NciI 777 CCCGG CC^[GC]GG
2252 CCCGG
NdeI 1229 CATATG CA^TATG
1547 CATATG
PflMI 490 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1564 CCATCTAATGG
PpuMI 2768 GGGTCCT [GA]G^G[AT]CC[CT]
3117 GGGACCC
Psp1406I 1714 AACGTT AA^CGTT
1921 AACGTT
SmlI 977 CTCAAG C^T[CT][GA]AG
2899 CTTGAG
StyI 2084 CCTTGG C^C[AT][AT]GG
3263 CCATGG
Van91I 490 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1564 CCATCTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BglII
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII