(Circular) (Six-base) MAPSORT of: pFN21ASDA1562.seq Check: 4983 from: 1 to: 8134 pFN21ASDA1562 8134 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5944 278 Size: 53 83 186 936 4408 2468 Fragments arranged by size: 4408 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5477 1937 Size: 3540 4594 Acc65I G'GTAC_C Cuts at: 5455 5455 Size: 8134 AclI AA'CG_TT Cuts at: 1563 3738 3945 6261 6634 1563 Size: 2175 207 2316 373 3063 Fragments arranged by size: 3063 2316 2175 373 207 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2648 2666 2860 3227 4058 4074 6197 7581 Size: 1016 18 194 367 831 16 2123 1384 Cuts at: 7581 7849 1632 Size: 268 1917 Fragments arranged by size: 2123 1917 1384 1016 831 367 268 194 18 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7937 AflIII A'CryG_T Cuts at: 1203 4860 7033 8010 1203 Size: 3657 2173 977 1327 Fragments arranged by size: 3657 2173 1327 977 AgeI A'CCGG_T Cuts at: 6965 6965 Size: 8134 AhdI GACnn_n'nnGTC Cuts at: 3351 6863 3351 Size: 3512 4622 AleI CACnn'nnGTG Cuts at: 1166 3650 3707 4304 5253 1166 Size: 2484 57 597 949 4047 Fragments arranged by size: 4047 2484 949 597 57 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8134 AlwNI CAG_nnn'CTG Cuts at: 3497 5877 7449 3497 Size: 2380 1572 4182 Fragments arranged by size: 4182 2380 1572 ApaLI G'TGCA_C Cuts at: 6191 7347 6191 Size: 1156 6978 ApoI r'AATT_y Cuts at: 1493 2107 2239 2527 3016 3434 4468 4622 4752 Size: 614 132 288 489 418 1034 154 130 Cuts at: 4752 4880 5443 5669 5821 7930 1493 Size: 128 563 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 614 563 489 418 288 226 154 152 132 130 128 AseI AT'TA_AT Cuts at: 160 1032 2412 6688 160 Size: 872 1380 4276 1606 Fragments arranged by size: 4276 1606 1380 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8134 AvaI C'yCGr_G Cuts at: 1870 1943 5459 1870 Size: 73 3516 4545 Fragments arranged by size: 4545 3516 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3141 3174 3141 Size: 33 8101 BamHI G'GATC_C Cuts at: 2836 3139 5464 5831 2836 Size: 303 2325 367 5139 Fragments arranged by size: 5139 2325 367 303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4306 5455 6910 618 Size: 325 217 627 2519 1149 1455 1842 Fragments arranged by size: 2519 1842 1455 1149 627 325 217 BanII G_rGCy'C Cuts at: 1391 5453 1391 Size: 4062 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3254 4833 928 Size: 971 1355 1579 4229 Fragments arranged by size: 4229 1579 1355 971 BbvCI CC'TCA_GC Cuts at: 3333 4014 3333 Size: 681 7453 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6325 6359 1903 Size: 34 4388 34 3678 Fragments arranged by size: 4388 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5008 6026 7236 5008 Size: 1018 1210 5906 Fragments arranged by size: 5906 1210 1018 BclI T'GATC_A Cuts at: 1548 2352 3622 1548 Size: 804 1270 6060 Fragments arranged by size: 6060 1270 804 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 6745 136 Size: 107 122 71 631 605 2604 2469 1525 Fragments arranged by size: 2604 2469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2438 2821 3305 4337 8129 2438 Size: 383 484 1032 3792 2443 Fragments arranged by size: 3792 2443 1032 484 383 BlpI GC'TnA_GC Cuts at: 4935 5551 4935 Size: 616 7518 Bme1580I G_kGCm'C Cuts at: 1790 6195 7351 1790 Size: 4405 1156 2573 Fragments arranged by size: 4405 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8134 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 2422 3345 Size: 416 53 83 399 24 691 314 923 Cuts at: 3345 5019 5865 6823 442 Size: 1674 846 958 1753 Fragments arranged by size: 1753 1674 958 923 846 691 416 399 314 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8134 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4489 4531 4489 Size: 42 8092 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5147 5236 6794 1239 Size: 3908 89 1558 2579 Fragments arranged by size: 3908 2579 1558 89 Bpu10I CC'TnA_GC Cuts at: 3333 4014 3333 Size: 681 7453 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2985 4943 6259 7124 7422 2985 Size: 1958 1316 865 298 3697 Fragments arranged by size: 3697 1958 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4519 6797 882 Size: 617 3020 2278 2219 Fragments arranged by size: 3020 2278 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4459 5431 5925 493 Size: 711 3255 972 494 2702 Fragments arranged by size: 3255 2702 972 711 494 BsaBI GATnn'nnATC Cuts at: 3498 8128 3498 Size: 4630 3504 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5941 6323 6958 275 Size: 53 83 186 936 4408 382 635 1451 Fragments arranged by size: 4408 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4949 6567 6965 7239 7386 7797 4949 Size: 1618 398 274 147 411 5286 Fragments arranged by size: 5286 1618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2633 2663 3192 3222 1178 Size: 30 165 30 1230 30 529 30 6090 Fragments arranged by size: 6090 1230 529 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2235 3911 4796 5306 1186 Size: 1049 1676 885 510 4014 Fragments arranged by size: 4014 1676 1049 885 510 BseYI C'CCAG_C Cuts at: 3025 4896 7337 7833 3025 Size: 1871 2441 496 3326 Fragments arranged by size: 3326 2441 1871 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2490 4173 4759 4807 5279 1163 Size: 573 754 1683 586 48 472 4018 Fragments arranged by size: 4018 1683 754 586 573 472 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5584 6345 7373 1540 Size: 275 186 3583 761 1028 2301 Fragments arranged by size: 3583 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3714 5337 6195 6280 7351 3714 Size: 1623 858 85 1071 4497 Fragments arranged by size: 4497 1623 1071 858 85 BsmI GAATG_Cn' Cuts at: 2041 2487 2833 3865 5102 5645 5738 2041 Size: 446 346 1032 1237 543 93 4437 Fragments arranged by size: 4437 1237 1032 543 446 346 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4359 5069 4359 Size: 710 7424 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3714 5337 5453 6195 6280 7351 1391 Size: 399 1924 1623 116 742 85 1071 2174 Fragments arranged by size: 2174 1924 1623 1071 742 399 116 85 BspEI T'CCGG_A Cuts at: 7797 7797 Size: 8134 BspHI T'CATG_A Cuts at: 3625 6023 7753 3625 Size: 2398 1730 4006 Fragments arranged by size: 4006 2398 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3878 4501 5489 844 Size: 3034 623 988 3489 Fragments arranged by size: 3489 3034 988 623 BsrBI CCG'CTC Cuts at: 1394 1823 6021 1394 Size: 429 4198 3507 Fragments arranged by size: 4198 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3031 3761 4089 6629 6803 65 Size: 1183 1783 730 328 2540 174 1396 Fragments arranged by size: 2540 1783 1396 1183 730 328 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4398 6778 6965 1700 Size: 110 2588 2380 187 2869 Fragments arranged by size: 2869 2588 2380 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8134 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8134 BssSI C'ACGA_G Cuts at: 6194 7206 6194 Size: 1012 7122 BstAPI GCAn_nnn'nTGC Cuts at: 794 4151 794 Size: 3357 4777 BstBI TT'CG_AA Cuts at: 2007 6938 2007 Size: 4931 3203 BstEII G'GTnAC_C Cuts at: 1184 5165 1184 Size: 3981 4153 BstXI CCAn_nnnn'nTGG Cuts at: 2902 5125 5161 5383 2902 Size: 2223 36 222 5653 Fragments arranged by size: 5653 2223 222 36 BstYI r'GATC_y Cuts at: 1972 2438 2821 2836 3139 3305 3493 4217 4337 Size: 466 383 15 303 166 188 724 120 Cuts at: 4337 5295 5464 5831 6216 6233 7685 7771 7783 Size: 958 169 367 385 17 1452 86 12 Cuts at: 7783 8129 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 958 724 466 385 383 367 346 303 188 169 166 120 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 4138 4138 Size: 8134 BtgI C'CryG_G Cuts at: 513 1065 2832 4645 5286 513 Size: 552 1767 1813 641 3361 Fragments arranged by size: 3361 1813 1767 641 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7395 Fragments arranged by size: 7395 683 56 BtsI GCAGTG_nn' Cuts at: 2670 4161 4826 5056 5107 5650 6444 6464 2670 Size: 1491 665 230 51 543 794 20 4340 Fragments arranged by size: 4340 1491 794 665 543 230 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8134 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8099 DraI TTT'AAA Cuts at: 2914 3238 5438 2914 Size: 324 2200 5610 Fragments arranged by size: 5610 2200 324 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7141 809 Size: 507 5825 1802 Fragments arranged by size: 5825 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4236 4642 5581 Size: 54 1425 50 275 18 2406 406 939 Cuts at: 5581 6470 8099 8 Size: 889 1629 43 Fragments arranged by size: 2406 1629 1425 939 889 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5581 1537 Size: 275 3769 4090 Fragments arranged by size: 4090 3769 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4958 6064 1353 Size: 3605 1106 3423 Fragments arranged by size: 3605 3423 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5408 6721 7095 7241 387 Size: 1064 138 3819 1313 374 146 1280 Fragments arranged by size: 3819 1313 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2648 2666 2860 3227 4058 4074 5147 Size: 393 1016 18 194 367 831 16 1073 Cuts at: 5147 5236 6197 6794 7581 7849 1239 Size: 89 961 597 787 268 1524 Fragments arranged by size: 1524 1073 1016 961 831 787 597 393 367 268 194 89 18 16 EcoO109I rG'GnC_Cy Cuts at: 3755 4792 5141 3755 Size: 1037 349 6748 Fragments arranged by size: 6748 1037 349 EcoRI G'AATT_C Cuts at: 4752 4880 5443 5821 4752 Size: 128 563 378 7065 Fragments arranged by size: 7065 563 378 128 EcoRV GAT'ATC Cuts at: 2460 3394 2460 Size: 934 7200 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5161 5161 Size: 8134 FspI TGC'GCA Cuts at: 6640 6640 Size: 8134 HaeII r_GCGC'y Cuts at: 1678 2853 7281 1678 Size: 1175 4428 2531 Fragments arranged by size: 4428 2531 1175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2665 2753 2785 3337 Size: 32 135 32 1061 32 88 32 552 Cuts at: 3337 3369 3833 3865 3908 3940 4202 4234 6775 Size: 32 464 32 43 32 262 32 2541 Cuts at: 6775 6807 6849 6881 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2541 1061 552 464 262 135 88 43 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5478 5724 669 Size: 988 281 3540 246 3079 Fragments arranged by size: 3540 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2192 3215 748 Size: 1444 1023 5667 Fragments arranged by size: 5667 1444 1023 HpaI GTT'AAC Cuts at: 5724 5724 Size: 8134 KpnI G_GTAC'C Cuts at: 5459 5459 Size: 8134 MfeI C'AATT_G Cuts at: 2295 3837 5733 2295 Size: 1542 1896 4696 Fragments arranged by size: 4696 1896 1542 MluI A'CGCG_T Cuts at: 8010 8010 Size: 8134 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2122 5142 7248 7432 1305 Size: 817 3020 2106 184 2007 Fragments arranged by size: 3020 2106 2007 817 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6312 Fragments arranged by size: 6312 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3225 3650 3707 3925 4304 5253 5346 Size: 648 2059 425 57 218 379 949 93 Cuts at: 5346 6092 6451 6610 518 Size: 746 359 159 2042 Fragments arranged by size: 2059 2042 949 746 648 425 379 359 218 159 93 57 MspA1I CmG'CkG Cuts at: 1606 3873 5550 6227 7375 7620 1606 Size: 2267 1677 677 1148 245 2120 Fragments arranged by size: 2267 2120 1677 1148 677 245 NaeI GCC'GGC Cuts at: 4400 4400 Size: 8134 NcoI C'CATG_G Cuts at: 513 1065 5286 513 Size: 552 4221 3361 Fragments arranged by size: 4221 3361 552 NdeI CA'TA_TG Cuts at: 387 3253 3571 387 Size: 2866 318 4950 Fragments arranged by size: 4950 2866 318 NgoMIV G'CCGG_C Cuts at: 4398 4398 Size: 8134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8134 NotI GC'GGCC_GC Cuts at: 5581 5581 Size: 8134 NspI r_CATG'y Cuts at: 1132 1229 4864 5492 7037 1132 Size: 97 3635 628 1545 2229 Fragments arranged by size: 3635 2229 1545 628 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8134 PciI A'CATG_T Cuts at: 4860 7033 4860 Size: 2173 5961 PflMI CCAn_nnn'nTGG Cuts at: 1108 2519 3593 1108 Size: 1411 1074 5649 Fragments arranged by size: 5649 1411 1074 PfoI T'CCnGG_A Cuts at: 4586 4586 Size: 8134 PmeI GTTT'AAAC Cuts at: 5438 5438 Size: 8134 PmlI CAC'GTG Cuts at: 4459 4459 Size: 8134 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6203 6235 7716 7748 6203 Size: 32 1481 32 6589 Fragments arranged by size: 6589 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4792 5141 4792 Size: 349 7785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8134 PsiI TTA'TAA Cuts at: 3044 5704 3044 Size: 2660 5474 PstI C_TGCA'G Cuts at: 830 2379 4232 4945 5486 830 Size: 1549 1853 713 541 3478 Fragments arranged by size: 3478 1853 1549 713 541 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8134 PvuII CAG'CTG Cuts at: 3873 3873 Size: 8134 SalI G'TCGA_C Cuts at: 1936 5476 1936 Size: 3540 4594 SanDI GG'GwC_CC Cuts at: 5141 5141 Size: 8134 SbfI CC_TGCA'GG Cuts at: 5486 5486 Size: 8134 ScaI AGT'ACT Cuts at: 6382 8094 6382 Size: 1712 6422 SfcI C'TryA_G Cuts at: 826 1045 2375 3360 4228 4594 4941 5482 6617 Size: 219 1330 985 868 366 347 541 1135 Cuts at: 6617 7298 7489 826 Size: 681 191 1471 Fragments arranged by size: 1471 1330 1135 985 868 681 541 366 347 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8134 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8134 SmaI CCC'GGG Cuts at: 1872 5461 1872 Size: 3589 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3000 4922 6238 7139 7401 820 Size: 197 926 1057 1922 1316 901 262 1553 Fragments arranged by size: 1922 1553 1316 1057 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5431 5925 493 Size: 4938 494 2702 Fragments arranged by size: 4938 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8134 SphI G_CATG'C Cuts at: 1132 5492 1132 Size: 4360 3774 SspI AAT'ATT Cuts at: 5 52 4583 6058 5 Size: 47 4531 1475 2081 Fragments arranged by size: 4531 2081 1475 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8134 StyI C'CwwG_G Cuts at: 513 1065 4107 5286 5573 513 Size: 552 3042 1179 287 3074 Fragments arranged by size: 3074 3042 1179 552 287 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3364 4275 4502 4703 5103 6161 6178 Size: 1255 1784 911 227 201 400 1058 17 Cuts at: 6178 6331 6516 6961 325 Size: 153 185 445 1498 Fragments arranged by size: 1784 1498 1255 1058 911 445 400 227 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2095 4076 6380 Size: 274 80 33 51 1443 118 1981 2304 Cuts at: 6380 8092 96 Size: 1712 138 Fragments arranged by size: 2304 1981 1712 1443 274 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3399 3431 8094 8126 3399 Size: 32 4663 32 3407 Fragments arranged by size: 4663 3407 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3228 1316 Size: 1912 6222 XbaI T'CTAG_A Cuts at: 5470 5470 Size: 8134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3949 5348 5383 3949 Size: 1399 35 6700 Fragments arranged by size: 6700 1399 35 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8134 XmaI C'CCGG_G Cuts at: 1870 5459 1870 Size: 3589 4545 XmnI GAAnn'nnTTC Cuts at: 3905 6263 3905 Size: 2358 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5942 276 Size: 53 83 186 936 4408 2468 Fragments arranged by size: 4408 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI