Restriction map for
pFN21ASDA1562
(Linear) (Six-base) MAPSORT of: KIAA1562.seq Check: 9742 from: 1 to: 3405
KIAA1562.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AclI AA'CG_TT
Cuts at: 0 1715 1922 3405
Size: 1715 207 1483
Fragments arranged by size:
1715 1483 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 643 837 1204 2035 2051 3405
Size: 625 18 194 367 831 16 1354
Fragments arranged by size:
1354 831 625 367 194 18 16
AflIII A'CryG_T
Cuts at: 0 2837 3405
Size: 2837 568
AhdI GACnn_n'nnGTC
Cuts at: 0 1328 3405
Size: 1328 2077
AleI CACnn'nnGTG
Cuts at: 0 1627 1684 2281 3230 3405
Size: 1627 57 597 949 175
Fragments arranged by size:
1627 949 597 175 57
AlwNI CAG_nnn'CTG
Cuts at: 0 1474 3405
Size: 1474 1931
ApoI r'AATT_y
Cuts at: 0 84 216 504 993 1411 2445 2599 2729
Size: 84 132 288 489 418 1034 154 130
Cuts at: 2729 2857 3405
Size: 128 548
Fragments arranged by size:
1034 548 489 418 288 154 132 130
128 84
AseI AT'TA_AT
Cuts at: 0 389 3405
Size: 389 3016
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1118 1151 3405
Size: 1118 33 2254
Fragments arranged by size:
2254 1118 33
BamHI G'GATC_C
Cuts at: 0 813 1116 3405
Size: 813 303 2289
Fragments arranged by size:
2289 813 303
BanI G'GyrC_C
Cuts at: 0 2283 3405
Size: 2283 1122
BbsI GAAGACnn'nnnn_
Cuts at: 0 1231 2810 3405
Size: 1231 1579 595
Fragments arranged by size:
1579 1231 595
BbvCI CC'TCA_GC
Cuts at: 0 1310 1991 3405
Size: 1310 681 1414
Fragments arranged by size:
1414 1310 681
BciVI GTATCCnnnnn_n'
Cuts at: 0 2985 3405
Size: 2985 420
BclI T'GATC_A
Cuts at: 0 329 1599 3405
Size: 329 1270 1806
Fragments arranged by size:
1806 1270 329
BglI GCCn_nnn'nGGC
Cuts at: 0 2253 3405
Size: 2253 1152
BglII A'GATC_T
Cuts at: 0 415 798 1282 2314 3405
Size: 415 383 484 1032 1091
Fragments arranged by size:
1091 1032 484 415 383
BlpI GC'TnA_GC
Cuts at: 0 2912 3405
Size: 2912 493
BmrI ACTGGGnnnn_n'
Cuts at: 0 85 399 1322 2996 3405
Size: 85 314 923 1674 409
Fragments arranged by size:
1674 923 409 314 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2466 2508 3405
Size: 2466 42 897
Fragments arranged by size:
2466 897 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3124 3213 3405
Size: 3124 89 192
Fragments arranged by size:
3124 192 89
Bpu10I CC'TnA_GC
Cuts at: 0 1310 1991 3405
Size: 1310 681 1414
Fragments arranged by size:
1414 1310 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 2920 3405
Size: 962 1958 485
Fragments arranged by size:
1958 962 485
BsaI GGTCTCn'nnnn_
Cuts at: 0 2496 3405
Size: 2496 909
BsaAI yAC'GTr
Cuts at: 0 2436 3405
Size: 2436 969
BsaBI GATnn'nnATC
Cuts at: 0 1475 3405
Size: 1475 1930
BsaWI w'CCGG_w
Cuts at: 0 2926 3405
Size: 2926 479
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 610 640 1169 1199 3405
Size: 610 30 529 30 2206
Fragments arranged by size:
2206 610 529 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 212 1888 2773 3283 3405
Size: 212 1676 885 510 122
Fragments arranged by size:
1676 885 510 212 122
BseYI C'CCAG_C
Cuts at: 0 1002 2873 3405
Size: 1002 1871 532
Fragments arranged by size:
1871 1002 532
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 2150 2736 2784 3256 3405
Size: 467 1683 586 48 472 149
Fragments arranged by size:
1683 586 472 467 149 48
BsiHKAI G_wGCw'C
Cuts at: 0 1691 3314 3405
Size: 1691 1623 91
Fragments arranged by size:
1691 1623 91
BsmI GAATG_Cn'
Cuts at: 0 18 464 810 1842 3079 3405
Size: 18 446 346 1032 1237 326
Fragments arranged by size:
1237 1032 446 346 326 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2336 3046 3405
Size: 2336 710 359
Fragments arranged by size:
2336 710 359
Bsp1286I G_dGCh'C
Cuts at: 0 1691 3314 3405
Size: 1691 1623 91
Fragments arranged by size:
1691 1623 91
BspHI T'CATG_A
Cuts at: 0 1602 3405
Size: 1602 1803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1855 2478 3405
Size: 1855 623 927
Fragments arranged by size:
1855 927 623
BsrDI GCAATG_nn'
Cuts at: 0 1008 1738 2066 3405
Size: 1008 730 328 1339
Fragments arranged by size:
1339 1008 730 328
BsrFI r'CCGG_y
Cuts at: 0 2375 3405
Size: 2375 1030
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2128 3405
Size: 2128 1277
BstEII G'GTnAC_C
Cuts at: 0 3142 3405
Size: 3142 263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 879 3102 3138 3360 3405
Size: 879 2223 36 222 45
Fragments arranged by size:
2223 879 222 45 36
BstYI r'GATC_y
Cuts at: 0 415 798 813 1116 1282 1470 2194 2314
Size: 415 383 15 303 166 188 724 120
Cuts at: 2314 3272 3405
Size: 958 133
Fragments arranged by size:
958 724 415 383 303 188 166 133
120 15
Bsu36I CC'TnA_GG
Cuts at: 0 2115 3405
Size: 2115 1290
BtgI C'CryG_G
Cuts at: 0 809 2622 3263 3405
Size: 809 1813 641 142
Fragments arranged by size:
1813 809 641 142
BtsI GCAGTG_nn'
Cuts at: 0 647 2138 2803 3033 3084 3405
Size: 647 1491 665 230 51 321
Fragments arranged by size:
1491 665 647 321 230 51
DraI TTT'AAA
Cuts at: 0 891 1215 3405
Size: 891 324 2190
Fragments arranged by size:
2190 891 324
EaeI y'GGCC_r
Cuts at: 0 2213 2619 3405
Size: 2213 406 786
Fragments arranged by size:
2213 786 406
EarI CTCTTCn'nnn_
Cuts at: 0 2935 3405
Size: 2935 470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3385 3405
Size: 3385 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 643 837 1204 2035 2051 3124 3213
Size: 625 18 194 367 831 16 1073 89
Cuts at: 3213 3405
Size: 192
Fragments arranged by size:
1073 831 625 367 194 192 89 18
16
EcoO109I rG'GnC_Cy
Cuts at: 0 1732 2769 3118 3405
Size: 1732 1037 349 287
Fragments arranged by size:
1732 1037 349 287
EcoRI G'AATT_C
Cuts at: 0 2729 2857 3405
Size: 2729 128 548
Fragments arranged by size:
2729 548 128
EcoRV GAT'ATC
Cuts at: 0 437 1371 3405
Size: 437 934 2034
Fragments arranged by size:
2034 934 437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3138 3405
Size: 3138 267
HaeII r_GCGC'y
Cuts at: 0 830 3405
Size: 830 2575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 730 762 1314 1346 1810 1842
Size: 610 32 88 32 552 32 464 32
Cuts at: 1842 1885 1917 2179 2211 3405
Size: 43 32 262 32 1194
Fragments arranged by size:
1194 610 552 464 262 88 43 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 169 1192 3405
Size: 169 1023 2213
Fragments arranged by size:
2213 1023 169
MfeI C'AATT_G
Cuts at: 0 272 1814 3405
Size: 272 1542 1591
Fragments arranged by size:
1591 1542 272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 3119 3405
Size: 99 3020 286
Fragments arranged by size:
3020 286 99
MslI CAynn'nnrTG
Cuts at: 0 1202 1627 1684 1902 2281 3230 3323 3405
Size: 1202 425 57 218 379 949 93 82
Fragments arranged by size:
1202 949 425 379 218 93 82 57
MspA1I CmG'CkG
Cuts at: 0 1850 3405
Size: 1850 1555
NaeI GCC'GGC
Cuts at: 0 2377 3405
Size: 2377 1028
NcoI C'CATG_G
Cuts at: 0 3263 3405
Size: 3263 142
NdeI CA'TA_TG
Cuts at: 0 1230 1548 3405
Size: 1230 318 1857
Fragments arranged by size:
1857 1230 318
NgoMIV G'CCGG_C
Cuts at: 0 2375 3405
Size: 2375 1030
NspI r_CATG'y
Cuts at: 0 2841 3405
Size: 2841 564
PciI A'CATG_T
Cuts at: 0 2837 3405
Size: 2837 568
PflMI CCAn_nnn'nTGG
Cuts at: 0 496 1570 3405
Size: 496 1074 1835
Fragments arranged by size:
1835 1074 496
PfoI T'CCnGG_A
Cuts at: 0 2563 3405
Size: 2563 842
PmlI CAC'GTG
Cuts at: 0 2436 3405
Size: 2436 969
PpuMI rG'GwC_Cy
Cuts at: 0 2769 3118 3405
Size: 2769 349 287
Fragments arranged by size:
2769 349 287
PsiI TTA'TAA
Cuts at: 0 1021 3405
Size: 1021 2384
PstI C_TGCA'G
Cuts at: 0 356 2209 2922 3405
Size: 356 1853 713 483
Fragments arranged by size:
1853 713 483 356
PvuII CAG'CTG
Cuts at: 0 1850 3405
Size: 1850 1555
SanDI GG'GwC_CC
Cuts at: 0 3118 3405
Size: 3118 287
SfcI C'TryA_G
Cuts at: 0 352 1337 2205 2571 2918 3405
Size: 352 985 868 366 347 487
Fragments arranged by size:
985 868 487 366 352 347
SmlI C'TyrA_G
Cuts at: 0 977 2899 3405
Size: 977 1922 506
Fragments arranged by size:
1922 977 506
SspI AAT'ATT
Cuts at: 0 2560 3405
Size: 2560 845
StyI C'CwwG_G
Cuts at: 0 2084 3263 3405
Size: 2084 1179 142
Fragments arranged by size:
2084 1179 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1341 2252 2479 2680 3080 3405
Size: 1341 911 227 201 400 325
Fragments arranged by size:
1341 911 400 325 227 201
TatI w'GTAC_w
Cuts at: 0 72 2053 3405
Size: 72 1981 1352
Fragments arranged by size:
1981 1352 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1376 1408 3405
Size: 1376 32 1997
Fragments arranged by size:
1997 1376 32
Tth111I GACn'n_nGTC
Cuts at: 0 1205 3405
Size: 1205 2200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1926 3325 3360 3405
Size: 1926 1399 35 45
Fragments arranged by size:
1926 1399 45 35
XmnI GAAnn'nnTTC
Cuts at: 0 1882 3405
Size: 1882 1523
Enzymes that do cut:
AclI AcuI AflIII AhdI AleI AlwNI ApoI AseI
BaeI BamHI BanI BbsI BbvCI BciVI BclI BglI
BglII BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HindIII MfeI MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI
PsiI PstI PvuII SanDI SfcI SmlI SspI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BanII
BbeI BcgI BfrBI Bme1580I BmgBI BmtI BsaHI BsiEI
BsiWI BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MscI NarI NheI NotI NruI NsiI PacI PasI
PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI XbaI XhoI XmaI ZraI