(Circular) (Six-base) MAPSORT of: pFN21ASDA1547.seq Check: 8863 from: 1 to: 7009 pFN21ASDA1547 7009 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2320 4086 2320 Size: 1766 5243 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3707 4819 278 Size: 53 83 186 936 2171 1112 2468 Fragments arranged by size: 2468 2171 1112 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3489 4352 1937 Size: 1552 863 4594 Fragments arranged by size: 4594 1552 863 Acc65I G'GTAC_C Cuts at: 4330 4330 Size: 7009 AclI AA'CG_TT Cuts at: 1563 5136 5509 1563 Size: 3573 373 3063 Fragments arranged by size: 3573 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5072 6456 6724 1632 Size: 3440 1384 268 1917 Fragments arranged by size: 3440 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6812 AflIII A'CryG_T Cuts at: 1203 5908 6885 1203 Size: 4705 977 1327 Fragments arranged by size: 4705 1327 977 AgeI A'CCGG_T Cuts at: 5840 5840 Size: 7009 AhdI GACnn_n'nnGTC Cuts at: 5738 5738 Size: 7009 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7009 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7009 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2011 2043 2011 Size: 32 6977 AlwNI CAG_nnn'CTG Cuts at: 2128 3209 3814 4265 4752 6324 2128 Size: 1081 605 451 487 1572 2813 Fragments arranged by size: 2813 1572 1081 605 487 451 ApaLI G'TGCA_C Cuts at: 5066 6222 5066 Size: 1156 5853 ApoI r'AATT_y Cuts at: 1493 2078 2117 4318 4544 4696 6805 1493 Size: 585 39 2201 226 152 2109 1697 Fragments arranged by size: 2201 2109 1697 585 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 5563 160 Size: 872 4531 1606 Fragments arranged by size: 4531 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7009 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2802 3406 4334 1870 Size: 73 117 742 604 928 4545 Fragments arranged by size: 4545 928 742 604 117 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2725 2758 2725 Size: 33 6976 BamHI G'GATC_C Cuts at: 4339 4706 4339 Size: 367 6642 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2531 3011 3091 3146 3875 Size: 325 217 627 744 480 80 55 729 Cuts at: 3875 4330 5785 618 Size: 455 1455 1842 Fragments arranged by size: 1842 1455 744 729 627 480 455 325 217 80 55 BanII G_rGCy'C Cuts at: 1391 2672 2967 3033 3551 4328 1391 Size: 1281 295 66 518 777 4072 Fragments arranged by size: 4072 1281 777 518 295 66 BbeI G_GCGC'C Cuts at: 2535 3095 3150 2535 Size: 560 55 6394 Fragments arranged by size: 6394 560 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2189 928 Size: 971 290 5748 Fragments arranged by size: 5748 971 290 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3610 3644 4111 4145 5200 5234 1903 Size: 34 1673 34 467 34 1055 34 3678 Fragments arranged by size: 3678 1673 1055 467 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2037 4901 6111 2037 Size: 2864 1210 2935 Fragments arranged by size: 2935 2864 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7009 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2891 3537 5620 Size: 107 122 71 631 605 1219 646 2083 Cuts at: 5620 136 Size: 1525 Fragments arranged by size: 2083 1525 1219 646 631 605 122 107 71 BglII A'GATC_T Cuts at: 3516 3969 7004 3516 Size: 453 3035 3521 Fragments arranged by size: 3521 3035 453 BlpI GC'TnA_GC Cuts at: 4426 4426 Size: 7009 Bme1580I G_kGCm'C Cuts at: 1790 3372 5070 6226 1790 Size: 1582 1698 1156 2573 Fragments arranged by size: 2573 1698 1582 1156 BmgBI CAC'GTC Cuts at: 1318 3487 1318 Size: 2169 4840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2489 2537 4005 Size: 416 53 83 399 24 1072 48 1468 Cuts at: 4005 4149 4740 5698 442 Size: 144 591 958 1753 Fragments arranged by size: 1753 1468 1072 958 591 416 399 144 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7009 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 2459 2466 2991 3333 4186 5669 1239 Size: 1151 69 7 525 342 853 1483 2579 Fragments arranged by size: 2579 1483 1151 853 525 342 69 7 Bpu10I CC'TnA_GC Cuts at: 3032 3032 Size: 7009 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2746 2951 3277 4088 4240 5134 5999 6297 2746 Size: 205 326 811 152 894 865 298 3458 Fragments arranged by size: 3458 894 865 811 326 298 205 152 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 5672 882 Size: 617 978 3195 2219 Fragments arranged by size: 3195 2219 978 617 BsaAI yAC'GTr Cuts at: 493 1204 4306 4800 493 Size: 711 3102 494 2702 Fragments arranged by size: 3102 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7003 7003 Size: 7009 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2532 2997 3092 3147 Size: 53 83 186 936 999 465 95 55 Cuts at: 3147 3384 3704 3747 4172 4816 5198 5833 275 Size: 237 320 43 425 644 382 635 1451 Fragments arranged by size: 1451 999 936 644 635 465 425 382 320 237 186 95 83 55 53 43 BsaWI w'CCGG_w Cuts at: 5442 5840 6114 6261 6672 5442 Size: 398 274 147 411 5779 Fragments arranged by size: 5779 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2936 2966 3437 3467 3578 Size: 30 165 30 1533 30 471 30 111 Cuts at: 3578 3608 1178 Size: 30 4579 Fragments arranged by size: 4579 1533 471 165 111 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2726 1186 Size: 1540 5469 BseYI C'CCAG_C Cuts at: 3558 3979 6212 6708 3558 Size: 421 2233 496 3859 Fragments arranged by size: 3859 2233 496 421 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3785 4028 4058 4064 1163 Size: 573 2049 243 30 6 4108 Fragments arranged by size: 4108 2049 573 243 30 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3224 4459 5220 6248 1540 Size: 275 186 1223 1235 761 1028 2301 Fragments arranged by size: 2301 1235 1223 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 4098 4139 5070 5155 6226 2322 Size: 1776 41 931 85 1071 3105 Fragments arranged by size: 3105 1776 1071 931 85 41 BsmI GAATG_Cn' Cuts at: 4520 4613 4520 Size: 93 6916 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2672 2967 3033 3372 3551 4098 Size: 399 532 350 295 66 339 179 547 Cuts at: 4098 4139 4328 5070 5155 6226 1391 Size: 41 189 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 532 399 350 339 295 189 179 85 66 41 BspEI T'CCGG_A Cuts at: 6672 6672 Size: 7009 BspHI T'CATG_A Cuts at: 4898 6628 4898 Size: 1730 5279 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2320 2350 3807 3933 4086 4364 844 Size: 1476 30 1457 126 153 278 3489 Fragments arranged by size: 3489 1476 1457 278 153 126 30 BsrBI CCG'CTC Cuts at: 1394 1823 3594 4896 1394 Size: 429 1771 1302 3507 Fragments arranged by size: 3507 1771 1302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3783 5504 5678 65 Size: 1183 2535 1721 174 1396 Fragments arranged by size: 2535 1721 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3043 3155 5653 5840 1700 Size: 110 1233 112 2498 187 2869 Fragments arranged by size: 2869 2498 1233 187 112 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7009 BssHII G'CGCG_C Cuts at: 1925 2350 1925 Size: 425 6584 BssSI C'ACGA_G Cuts at: 3478 4082 5069 6081 3478 Size: 604 987 1012 4406 Fragments arranged by size: 4406 1012 987 604 BstAPI GCAn_nnn'nTGC Cuts at: 794 3209 3367 3507 794 Size: 2415 158 140 4296 Fragments arranged by size: 4296 2415 158 140 BstBI TT'CG_AA Cuts at: 2007 5813 2007 Size: 3806 3203 BstEII G'GTnAC_C Cuts at: 1184 2362 1184 Size: 1178 5831 BstXI CCAn_nnnn'nTGG Cuts at: 3842 3842 Size: 7009 BstYI r'GATC_y Cuts at: 1972 3516 3969 4339 4706 5091 5108 6560 6646 Size: 1544 453 370 367 385 17 1452 86 Cuts at: 6646 6658 7004 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1544 1452 453 385 370 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3439 513 Size: 552 2374 4083 Fragments arranged by size: 4083 2374 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3789 487 Size: 683 56 2563 3707 Fragments arranged by size: 3707 2563 683 56 BtsI GCAGTG_nn' Cuts at: 2349 4525 5319 5339 2349 Size: 2176 794 20 4019 Fragments arranged by size: 4019 2176 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7009 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6974 DraI TTT'AAA Cuts at: 2077 4313 2077 Size: 2236 4773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3752 6016 809 Size: 507 2436 2264 1802 Fragments arranged by size: 2436 2264 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 3732 4456 Size: 54 1425 50 275 18 1387 515 724 Cuts at: 4456 5345 6974 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1387 889 724 515 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4456 1537 Size: 275 2644 4090 Fragments arranged by size: 4090 2644 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2902 4939 1353 Size: 911 638 2037 3423 Fragments arranged by size: 3423 2037 911 638 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2723 5596 5970 6116 387 Size: 1064 138 1134 2873 374 146 1280 Fragments arranged by size: 2873 1280 1134 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2459 2466 2991 3333 4186 5072 Size: 393 758 69 7 525 342 853 886 Cuts at: 5072 5669 6456 6724 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 886 853 787 758 597 525 393 342 268 69 7 EcoNI CCTnn'n_nnAGG Cuts at: 3929 3929 Size: 7009 EcoO109I rG'GnC_Cy Cuts at: 2788 3395 3664 3796 3895 3922 2788 Size: 607 269 132 99 27 5875 Fragments arranged by size: 5875 607 269 132 99 27 EcoRI G'AATT_C Cuts at: 2117 4318 4696 2117 Size: 2201 378 4430 Fragments arranged by size: 4430 2201 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 4169 2740 Size: 1429 5580 FspI TGC'GCA Cuts at: 3059 5515 3059 Size: 2456 4553 HaeII r_GCGC'y Cuts at: 1678 2200 2535 3095 3150 3189 6156 1678 Size: 522 335 560 55 39 2967 2531 Fragments arranged by size: 2967 2531 560 522 335 55 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 2746 2778 2936 Size: 32 135 32 1019 32 123 32 158 Cuts at: 2936 2968 3656 3688 3689 3721 5650 5682 5724 Size: 32 688 32 1 32 1929 32 42 Cuts at: 5724 5756 1373 Size: 32 2626 Fragments arranged by size: 2626 1929 1019 688 158 135 123 42 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 4353 4599 669 Size: 988 281 2415 246 3079 Fragments arranged by size: 3079 2415 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7009 HpaI GTT'AAC Cuts at: 4599 4599 Size: 7009 KasI G'GCGC_C Cuts at: 2531 3091 3146 2531 Size: 560 55 6394 Fragments arranged by size: 6394 560 55 KpnI G_GTAC'C Cuts at: 4334 4334 Size: 7009 MfeI C'AATT_G Cuts at: 4608 4608 Size: 7009 MluI A'CGCG_T Cuts at: 6885 6885 Size: 7009 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2997 6123 6307 1305 Size: 1692 3126 184 2007 Fragments arranged by size: 3126 2007 1692 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3734 10 Size: 54 1425 343 1902 3285 Fragments arranged by size: 3285 1902 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 3342 3840 4967 5326 5485 518 Size: 648 1077 1099 498 1127 359 159 2042 Fragments arranged by size: 2042 1127 1099 1077 648 498 359 159 MspA1I CmG'CkG Cuts at: 1606 3197 3209 3562 3852 4072 4238 4425 5102 Size: 1591 12 353 290 220 166 187 677 Cuts at: 5102 6250 6495 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1591 1148 677 353 290 245 220 187 166 12 NaeI GCC'GGC Cuts at: 3045 3157 3045 Size: 112 6897 NarI GG'CG_CC Cuts at: 2532 3092 3147 2532 Size: 560 55 6394 Fragments arranged by size: 6394 560 55 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6457 NdeI CA'TA_TG Cuts at: 387 387 Size: 7009 NgoMIV G'CCGG_C Cuts at: 3043 3155 3043 Size: 112 6897 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7009 NotI GC'GGCC_GC Cuts at: 4456 4456 Size: 7009 NspI r_CATG'y Cuts at: 1132 1229 4367 5912 1132 Size: 97 3138 1545 2229 Fragments arranged by size: 3138 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2536 2960 3026 1348 Size: 1188 424 66 5331 Fragments arranged by size: 5331 1188 424 66 PciI A'CATG_T Cuts at: 5908 5908 Size: 7009 PflMI CCAn_nnn'nTGG Cuts at: 1108 3814 3885 1108 Size: 2706 71 4232 Fragments arranged by size: 4232 2706 71 PfoI T'CCnGG_A Cuts at: 2482 3310 2482 Size: 828 6181 PmeI GTTT'AAAC Cuts at: 4313 4313 Size: 7009 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5078 5110 6591 6623 5078 Size: 32 1481 32 5464 Fragments arranged by size: 5464 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 3664 3796 3922 2788 Size: 876 132 126 5875 Fragments arranged by size: 5875 876 132 126 PshAI GACnn'nnGTC Cuts at: 1613 2934 3815 1613 Size: 1321 881 4807 Fragments arranged by size: 4807 1321 881 PsiI TTA'TAA Cuts at: 4579 4579 Size: 7009 PstI C_TGCA'G Cuts at: 830 3205 3773 4361 830 Size: 2375 568 588 3478 Fragments arranged by size: 3478 2375 588 568 PvuI CG_AT'CG Cuts at: 2001 3224 2001 Size: 1223 5786 PvuII CAG'CTG Cuts at: 3562 3852 4072 3562 Size: 290 220 6499 Fragments arranged by size: 6499 290 220 SalI G'TCGA_C Cuts at: 1936 4351 1936 Size: 2415 4594 SbfI CC_TGCA'GG Cuts at: 4361 4361 Size: 7009 ScaI AGT'ACT Cuts at: 5257 6969 5257 Size: 1712 5297 SfcI C'TryA_G Cuts at: 826 1045 2680 3201 3769 3944 4357 5492 6173 Size: 219 1635 521 568 175 413 1135 681 Cuts at: 6173 6364 826 Size: 191 1471 Fragments arranged by size: 1635 1471 1135 681 568 521 413 219 191 175 SfoI GGC'GCC Cuts at: 2533 3093 3148 2533 Size: 560 55 6394 Fragments arranged by size: 6394 560 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7009 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7009 SmaI CCC'GGG Cuts at: 1872 2062 2804 4336 1872 Size: 190 742 1532 4545 Fragments arranged by size: 4545 1532 742 190 SmlI C'TyrA_G Cuts at: 820 1017 1943 2725 2966 3292 4103 4219 5113 Size: 197 926 782 241 326 811 116 894 Cuts at: 5113 6014 6276 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 894 811 782 326 262 241 197 116 SnaBI TAC'GTA Cuts at: 493 4306 4800 493 Size: 3813 494 2702 Fragments arranged by size: 3813 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7009 SphI G_CATG'C Cuts at: 1132 4367 1132 Size: 3235 3774 SspI AAT'ATT Cuts at: 5 52 4933 5 Size: 47 4881 2081 Fragments arranged by size: 4881 2081 47 StuI AGG'CCT Cuts at: 1517 3285 1517 Size: 1768 5241 StyI C'CwwG_G Cuts at: 513 1065 2932 2987 4448 513 Size: 552 1867 55 1461 3074 Fragments arranged by size: 3074 1867 1461 552 55 SwaI ATTT'AAAT Cuts at: 2077 2077 Size: 7009 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5036 5053 5206 5391 5836 325 Size: 1255 3456 17 153 185 445 1498 Fragments arranged by size: 3456 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 5255 6967 Size: 274 80 33 51 1443 234 3044 1712 Cuts at: 6967 96 Size: 138 Fragments arranged by size: 3044 1712 1443 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3437 3469 6969 7001 3437 Size: 32 3500 32 3445 Fragments arranged by size: 3500 3445 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7009 XbaI T'CTAG_A Cuts at: 4345 4345 Size: 7009 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3559 3664 3559 Size: 105 6904 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7009 XmaI C'CCGG_G Cuts at: 1870 2060 2802 4334 1870 Size: 190 742 1532 4545 Fragments arranged by size: 4545 1532 742 190 XmnI GAAnn'nnTTC Cuts at: 5138 5138 Size: 7009 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3705 4817 276 Size: 53 83 186 936 2171 1112 2468 Fragments arranged by size: 2468 2171 1112 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI