Restriction map for
pFN21ASDA1547
(Linear) (Six-base) MAPSORT of: KIAA1547.seq Check: 2123 from: 1 to: 2280
KIAA1547.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 297 2063 2280
Size: 297 1766 217
Fragments arranged by size:
1766 297 217
AatII G_ACGT'C
Cuts at: 0 1684 2280
Size: 1684 596
AccI GT'mk_AC
Cuts at: 0 1466 2280
Size: 1466 814
AlwNI CAG_nnn'CTG
Cuts at: 0 105 1186 1791 2242 2280
Size: 105 1081 605 451 38
Fragments arranged by size:
1081 605 451 105 38
ApoI r'AATT_y
Cuts at: 0 55 94 2280
Size: 55 39 2186
Fragments arranged by size:
2186 55 39
AvaI C'yCGr_G
Cuts at: 0 37 779 1383 2280
Size: 37 742 604 897
Fragments arranged by size:
897 742 604 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 702 735 2280
Size: 702 33 1545
Fragments arranged by size:
1545 702 33
BanI G'GyrC_C
Cuts at: 0 508 988 1068 1123 1852 2280
Size: 508 480 80 55 729 428
Fragments arranged by size:
729 508 480 428 80 55
BanII G_rGCy'C
Cuts at: 0 649 944 1010 1528 2280
Size: 649 295 66 518 752
Fragments arranged by size:
752 649 518 295 66
BbeI G_GCGC'C
Cuts at: 0 512 1072 1127 2280
Size: 512 560 55 1153
Fragments arranged by size:
1153 560 512 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 2280
Size: 166 2114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1587 1621 2088 2122 2280
Size: 1587 34 467 34 158
Fragments arranged by size:
1587 467 158 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 14 2280
Size: 14 2266
BglI GCCn_nnn'nGGC
Cuts at: 0 868 1514 2280
Size: 868 646 766
Fragments arranged by size:
868 766 646
BglII A'GATC_T
Cuts at: 0 1493 1946 2280
Size: 1493 453 334
Fragments arranged by size:
1493 453 334
Bme1580I G_kGCm'C
Cuts at: 0 1349 2280
Size: 1349 931
BmgBI CAC'GTC
Cuts at: 0 1464 2280
Size: 1464 816
BmrI ACTGGGnnnn_n'
Cuts at: 0 466 514 1982 2126 2280
Size: 466 48 1468 144 154
Fragments arranged by size:
1468 466 154 144 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 436 443 968 1310 2163 2280
Size: 367 69 7 525 342 853 117
Fragments arranged by size:
853 525 367 342 117 69 7
Bpu10I CC'TnA_GC
Cuts at: 0 1009 2280
Size: 1009 1271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 928 1254 2065 2217 2280
Size: 723 205 326 811 152 63
Fragments arranged by size:
811 723 326 205 152 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 2280
Size: 454 1826
BsaHI Gr'CG_yC
Cuts at: 0 509 974 1069 1124 1361 1681 1724 2149
Size: 509 465 95 55 237 320 43 425
Cuts at: 2149 2280
Size: 131
Fragments arranged by size:
509 465 425 320 237 131 95 55
43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 913 943 1414 1444 1555 1585 2280
Size: 913 30 471 30 111 30 695
Fragments arranged by size:
913 695 471 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 2280
Size: 703 1577
BseYI C'CCAG_C
Cuts at: 0 1535 1956 2280
Size: 1535 421 324
Fragments arranged by size:
1535 421 324
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1762 2005 2035 2041 2280
Size: 1762 243 30 6 239
Fragments arranged by size:
1762 243 239 30 6
BsiEI CG_ry'CG
Cuts at: 0 1201 2280
Size: 1201 1079
BsiHKAI G_wGCw'C
Cuts at: 0 299 2075 2116 2280
Size: 299 1776 41 164
Fragments arranged by size:
1776 299 164 41
Bsp1286I G_dGCh'C
Cuts at: 0 299 649 944 1010 1349 1528 2075 2116
Size: 299 350 295 66 339 179 547 41
Cuts at: 2116 2280
Size: 164
Fragments arranged by size:
547 350 339 299 295 179 164 66
41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 297 327 1784 1910 2063 2280
Size: 297 30 1457 126 153 217
Fragments arranged by size:
1457 297 217 153 126 30
BsrBI CCG'CTC
Cuts at: 0 1571 2280
Size: 1571 709
BsrDI GCAATG_nn'
Cuts at: 0 1760 2280
Size: 1760 520
BsrFI r'CCGG_y
Cuts at: 0 1020 1132 2280
Size: 1020 112 1148
Fragments arranged by size:
1148 1020 112
BssHII G'CGCG_C
Cuts at: 0 327 2280
Size: 327 1953
BssSI C'ACGA_G
Cuts at: 0 1455 2059 2280
Size: 1455 604 221
Fragments arranged by size:
1455 604 221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1186 1344 1484 2280
Size: 1186 158 140 796
Fragments arranged by size:
1186 796 158 140
BstEII G'GTnAC_C
Cuts at: 0 339 2280
Size: 339 1941
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1819 2280
Size: 1819 461
BstYI r'GATC_y
Cuts at: 0 1493 1946 2280
Size: 1493 453 334
Fragments arranged by size:
1493 453 334
BtgI C'CryG_G
Cuts at: 0 1416 2280
Size: 1416 864
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1766 2280
Size: 1766 514
BtsI GCAGTG_nn'
Cuts at: 0 326 2280
Size: 326 1954
DraI TTT'AAA
Cuts at: 0 54 2280
Size: 54 2226
DrdI GACnn_nn'nnGTC
Cuts at: 0 1729 2280
Size: 1729 551
EaeI y'GGCC_r
Cuts at: 0 1194 1709 2280
Size: 1194 515 571
Fragments arranged by size:
1194 571 515
EarI CTCTTCn'nnn_
Cuts at: 0 241 879 2280
Size: 241 638 1401
Fragments arranged by size:
1401 638 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 2280
Size: 700 1580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 436 443 968 1310 2163 2280
Size: 367 69 7 525 342 853 117
Fragments arranged by size:
853 525 367 342 117 69 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1906 2280
Size: 1906 374
EcoO109I rG'GnC_Cy
Cuts at: 0 765 1372 1641 1773 1872 1899 2280
Size: 765 607 269 132 99 27 381
Fragments arranged by size:
765 607 381 269 132 99 27
EcoRI G'AATT_C
Cuts at: 0 94 2280
Size: 94 2186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 717 2146 2280
Size: 717 1429 134
Fragments arranged by size:
1429 717 134
FspI TGC'GCA
Cuts at: 0 1036 2280
Size: 1036 1244
HaeII r_GCGC'y
Cuts at: 0 177 512 1072 1127 1166 2280
Size: 177 335 560 55 39 1114
Fragments arranged by size:
1114 560 335 177 55 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 723 755 913 945 1633 1665
Size: 568 32 123 32 158 32 688 32
Cuts at: 1665 1666 1698 2280
Size: 1 32 582
Fragments arranged by size:
688 582 568 158 123 32 32 32
32 32 1
KasI G'GCGC_C
Cuts at: 0 508 1068 1123 2280
Size: 508 560 55 1157
Fragments arranged by size:
1157 560 508 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 2280
Size: 974 1306
MscI TGG'CCA
Cuts at: 0 1711 2280
Size: 1711 569
MslI CAynn'nnrTG
Cuts at: 0 220 1319 1817 2280
Size: 220 1099 498 463
Fragments arranged by size:
1099 498 463 220
MspA1I CmG'CkG
Cuts at: 0 1174 1186 1539 1829 2049 2215 2280
Size: 1174 12 353 290 220 166 65
Fragments arranged by size:
1174 353 290 220 166 65 12
NaeI GCC'GGC
Cuts at: 0 1022 1134 2280
Size: 1022 112 1146
Fragments arranged by size:
1146 1022 112
NarI GG'CG_CC
Cuts at: 0 509 1069 1124 2280
Size: 509 560 55 1156
Fragments arranged by size:
1156 560 509 55
NgoMIV G'CCGG_C
Cuts at: 0 1020 1132 2280
Size: 1020 112 1148
Fragments arranged by size:
1148 1020 112
PasI CC'CwG_GG
Cuts at: 0 513 937 1003 2280
Size: 513 424 66 1277
Fragments arranged by size:
1277 513 424 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 1791 1862 2280
Size: 1791 71 418
Fragments arranged by size:
1791 418 71
PfoI T'CCnGG_A
Cuts at: 0 459 1287 2280
Size: 459 828 993
Fragments arranged by size:
993 828 459
PpuMI rG'GwC_Cy
Cuts at: 0 765 1641 1773 1899 2280
Size: 765 876 132 126 381
Fragments arranged by size:
876 765 381 132 126
PshAI GACnn'nnGTC
Cuts at: 0 911 1792 2280
Size: 911 881 488
Fragments arranged by size:
911 881 488
PstI C_TGCA'G
Cuts at: 0 1182 1750 2280
Size: 1182 568 530
Fragments arranged by size:
1182 568 530
PvuI CG_AT'CG
Cuts at: 0 1201 2280
Size: 1201 1079
PvuII CAG'CTG
Cuts at: 0 1539 1829 2049 2280
Size: 1539 290 220 231
Fragments arranged by size:
1539 290 231 220
SfcI C'TryA_G
Cuts at: 0 657 1178 1746 1921 2280
Size: 657 521 568 175 359
Fragments arranged by size:
657 568 521 359 175
SfoI GGC'GCC
Cuts at: 0 510 1070 1125 2280
Size: 510 560 55 1155
Fragments arranged by size:
1155 560 510 55
SmaI CCC'GGG
Cuts at: 0 39 781 2280
Size: 39 742 1499
Fragments arranged by size:
1499 742 39
SmlI C'TyrA_G
Cuts at: 0 702 943 1269 2080 2196 2280
Size: 702 241 326 811 116 84
Fragments arranged by size:
811 702 326 241 116 84
StuI AGG'CCT
Cuts at: 0 1262 2280
Size: 1262 1018
StyI C'CwwG_G
Cuts at: 0 909 964 2280
Size: 909 55 1316
Fragments arranged by size:
1316 909 55
SwaI ATTT'AAAT
Cuts at: 0 54 2280
Size: 54 2226
TatI w'GTAC_w
Cuts at: 0 188 2280
Size: 188 2092
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1414 1446 2280
Size: 1414 32 834
Fragments arranged by size:
1414 834 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1536 1641 2280
Size: 1536 105 639
Fragments arranged by size:
1536 639 105
XmaI C'CCGG_G
Cuts at: 0 37 779 2280
Size: 37 742 1501
Fragments arranged by size:
1501 742 37
ZraI GAC'GTC
Cuts at: 0 1682 2280
Size: 1682 598
Enzymes that do cut:
AarI AatII AccI AlwNI ApoI AvaI BaeI BanI
BanII BbeI BbsI BcgI BciVI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PasI PflMI PfoI PpuMI PshAI PstI PvuI PvuII
SfcI SfoI SmaI SmlI StuI StyI SwaI TatI
TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BbvCI BclI BfrBI BlpI BmtI BplI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BstBI BstZ17I Bsu36I ClaI CspCI DraIII EagI
EcoICRI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI TaqII Tth111I XbaI XhoI XmnI